• Proceedings of the KSME Conference
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• 2008.11a
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• pp.1690-1691
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• 2008

Micro cell stimulation device is interested in many researchers because it has several advantages such as saving time and reagents. We introduce new micro-bioreactor using micro bead and conduct cell stimulation experiments to verify effective time because cell have operated by cell-cycle (G1, S, G2, and M phase). Micro-bioreactor was made by soft lithography and CAPE (calf pulmonary artery endothelial cell) was cultured in PDMS (polydimethylsiloxane) micro device for 12 hour and cell starvation process was performed for 24 hours. Micro glass beads were rolled only by slating device every hour during 15 hour because of minimizing other stimulation force like flow and pressure. The result represents that cells under exposed under micro bead stimulation show higher growth rate than normal condition and earlier and later stimulation time are more effective.

• Journal of Sericultural and Entomological Science
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• v.53 no.2
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• pp.118-123
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• 2015

The structural transition of silk protein has been induced by various method including alcohol treatment. To know the effect of alcohol concentration on silk beads conformation, silk beads were prepared in different alcohol concentration (100%, 70%, and 50%) and then examined the infrared spectra of silk beads. Silk beads prepared in 100% alcohol showed at $1265cm^{-1}$ attributed ${\beta}-sheet$ conformation and did not showed a characteristic absorption peak from model drug. However, silk beads in 70% and 50% alcohol showed some peaks originated from model drug including $2933cm^{-1}$, $1069cm^{-1}$, and $973cm^{-1}$. These results means that the micro-environment of silk beads was affected by alcohol concentration.

• YAKHAK HOEJI
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• v.45 no.5
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• pp.536-544
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• 2001

increase the viability of oral typhoid vaccine during the passage through the castro-intes-tidal tract, numerous attempts have been made including the vaccine coating. However problems such as high death rate during the coating process and its instability in the gastric juice still remain to be solved. In this study, the oral vaccine was made as the micro-enteric beads by adding Salmomella typhi Ty21a cells to sodium alginate solution and spraying onto calcium chloride solution (ionotropic relation method). The vaccine showed more than 90% of its original viability after treating it for 1 hour in the artificial gastric juice (37$^{\circ}C$, 300 rpm). The clearance rate of the Ty21a in the liver and spleen of the mice orally administrated with coated Ty21a was similar to that of the mice intraperitoneally administrated with uncoated Ty21a. The peripheral blood lymphocytes (PBL) isolated from the mice orally administered with this vaccine produced 15.5 fold higher specific IgA antibody titer than that from the control mice administerd with saline solution. furthermore, the mice treated with the coated Ty21a had higher survival rates (50~87%) than the control mice treated with saline solution (0~10%) in the intraperitoneal challenge test with wild type S. typhi Ty21a cells. These results suggest that the alginate-based coating technique is effective to protect live Ty21a from acidic environments, and produces better intestinal immune responses thereby providing a potentially excellent oral typhoid vaccine.

• 한국정보디스플레이학회:학술대회논문집
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• 2009.10a
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• pp.711-712
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• 2009

The fabrication of micro-scale irregular holes by using PMMA (Poly Methyl Methacrylate) beads in the front dielectric layer was proposed to improve luminous efficacy of an ac plasma display panel. Through the firing process of front dielectric layer, bowl shaped holes with a depth of $2.5{\mu}m$, a top diameter of $8-12{\mu}m$, and a bottom diameter of $4-7{\mu}m$ were fabricated. The proposed ac plasma display panel with the irregular micro-scale holes improved the luminous efficacy by 18 % due to the decrease in the minimum sustain voltage.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.25 no.8
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• pp.657-663
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• 2012

The spherical mesoporous silica is synthesized and incorporated with CdSe/ZnS quantum dots(QDs) for preparing micro beads to detect toxic and bio-materials with high sensitivity. The spherical silica beads with the brunauer-emmett-telle(BET) average pore size of 15 nm were prepared with a ratio 1, 3, 5-trimethylbenzen, as a swelling agent, to the block-copolymer template surfactant of over 1 and under vigorous mixing condition. The surface of spherical mesoporous silica is modified using octadecylsilane for incorporating QDs. Based on photoluminescence(PL) spectra, the relative brightness of mesoporous silica beads incorporated with 10 nM of QDs is 79,000 times brighter than that of Rodamine 6 G.

• Proceedings of the KIEE Conference
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• 2002.11a
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• pp.60-63
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• 2002

This paper presents bead-based microbiocihps to detect and separate target proteins. Micro beads coated with capture proteins were introduced into a microchamber, and target proteins flowing across the chamber were bound and concentrated. The chip was connected with an external fluid system. Bead surfaces were double-coated with photo-cleavable linkers and capture proteins. The proteins bound on the beads were photo-separated under UV irradiation, and excited to be measured in fluorescence. $38{\sim}50{\mu}m$ sized polystyrene beads were used. SOGs(silicon-on-glass) were used to fabricate the microchip having glasses bonded on both sides. 100 ${\mu}m$ thick silicon channel was formed through silicon deep RIE process. The upper glass cover had holed through to have inlets and outlets fabricated by powder-blastings. In this study, biotin and streptavidin were used as capture proteins and detection proteins, respectively. The protein mixtures of streptavidin, HSA(human serum albumin) and ovalbumin were applied for selective detection test.

• International Journal of Industrial Entomology and Biomaterials
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• v.44 no.2
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• pp.29-36
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• 2022

Over the past decades, silk sericin (SS) received increasing attention in the academic and industrial fields. In nature, SS acts as a glue that holds the two strands of silk fibrils together. However, recent works suggest that SS might have a more diverse role during the silk spinning process, such as stabilizing the SF in the silk gland. On the other hand, the sericulture industry has been trying to find novel applications for SS discarded from the silk fabric manufacturing process. Recovery and refining of SS would be the first step of the recycling of SS. Using a proper solvent SS could be shaped into various forms, such as spherical beads, microparticles, fibers, and films. Moreover, the applicability of these SS materials has been investigated in various fields such as cosmetics, templates for nanomaterials, drug delivery, heavy metal adsorption, and enzyme immobilization.

• Journal of the Korean Geotechnical Society
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• v.27 no.11
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• pp.83-92
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• 2011

The random and heterogeneous pore structure is a significant factor that dominates physical and mechanical behaviors of soils such as fluid flow and geomechanical responses driven by loading. The characterization method using non-destructive testing such as micro X-ray CT technique which has a high resolution with micrometer unit allows to observe internal structure of soils. However, the application has been limited to qualitatively observe 2D and 3D CT images and to obtain the void ratio at macro-scale although the CT images contain enormous information of materials of interests. In this study, we constructed the 3D particle and pore structures based on sequentially taken 2D images of glass beads and quantitatively defined complex pore structure with void cell and void channel. This approach was enabled by implementing image processing techniques that include coordinate transformation, binarization, Delaunay Triangulation, and Euclidean Distance Transform. It was confirmed that the suggested algorithm allows to quantitatively evaluate the distribution of void cells and their connectivity of heterogeneous pore structures for glass beads.

• Proceedings of the Korean Vacuum Society Conference
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• 2012.08a
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• pp.386-386
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• 2012

For white light emitting diode (LED) applications, it has been reported that Y3Al5O12:Ce3+ (YAG:Ce) in nano-sized phosphor performs better than it does in micro-sized particles. This is because nano-sized YAG:Ce can reduce internal light scattering when coated onto a blue LED surface. Recently, there have been many reports on the synthesis of nano-sized YAG particles using bottom-up method, such as co-precipitation method, sol-gel process, hydrothermal method, solvothermal method, and glycothermal method. However, there has been no report using top-down method. Top-down method has advantages than bottom-up method, such as large scale production and easy control of doping concentration and particle size. Therefore, in this study, nano-sized YAG:Ce phosphors were synthesized by a high energy beads milling process with varying beads size, milling time and milling steps. The beads milling process was performed by Laboratory Mill MINICER with ZrO2 beads. The phase identity and morphology of nano-sized YAG:Ce were characterized by X-ray powder diffraction (XRD) and field-emission scanning electron microscopy (FESEM), respectively. By controlling beads size, milling time and milling steps, we synthesized a size-tunable and uniform nano-sized YAG:Ce phosphors which average diameters were 100, 85 and 40 nm, respectively. After milling, there was no impurity and all of the peaks were in good agreement with YAG (JCPDS No. 33-0040). Luminescence and quantum efficiency (QE) of nano-sized YAG:Ce phosphors were measured by fluorescence spectrometer and QE measuring instrument, respectively. The synthesized YAG:Ce absorbed light efficiently in the visible region of 400-500 nm, and showed single broadband emission peaked at 550 nm with 50% of QE. As a result, by considering above results, high energy beads milling process could be a facile and reproducible synthesis method for nano-sized YAG:Ce phosphors.

• Polymer(Korea)
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• v.32 no.4
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• pp.377-384
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• 2008

In this study, the microsphere silicone polymer beads were synthesized by UV irradiation and alkoxy hydrolysis. The coefficient of variation (CV) of microsphere silicone polymer beads were decreased with increasing UV intensity, reaction time. The mean particle diameter, refractive index, and pH value were $4.1{\mu}m$, 1.43 and 7.5, respectively. Also, the true and bulk specific gravity, moisture content were 1.30, and 0.40, below 2%. The mean particle diameter and CV were the lowest at 0.1 wt% hexamethyldisilazane (HMDS) and their roundnesses were $0.95{\sim}0.98{\mu}m$ values. The particle dispersion index of microsphere silicone polymer beads was 4.92 at 450 W, 90 min and the yield was increased to 11.3% at 20 wt% methyltrimethoxysilane (MTMS). The mean particle diameter was decreased with increasing the stirring rate and reaction temperature.