• Title/Summary/Keyword: miR-29a

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A study on patients' satisfaction of physical therapy services in public health center in the point of view of the elderly welfare policy (서울시 보건소 물리치료실 이용자의 만족도 조사 연구 - 노인복지 정책측면에서 -)

  • Yoon, Bum-Chul;Lee, Young-Jin;Bae, Jung-Mi;Kim, Nan-Su
    • Journal of Korean Physical Therapy Science
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    • v.5 no.1
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    • pp.557-571
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    • 1998
  • The purpose of the study is (i) to investigate the operating situation of physical therapy department in public health centers, (ii) to analyze the difference of elderly satisfaction from non-elderly satisfaction of physical therapy services among the public health center visitors, and finally (iii) to suggest facilitating improvement on programs that can supply the elderly health service through public health centers. A questionnaire survey was carried out 212 patients of 25 public health center in Seoul, Korea from 15 July to 10 August 1996. Major results of the study are as follows : 1. Among the respondents. the largest group was the elderly who are over 65 years old (72.6%). 2. The averge of general patients' satisfaction for physical therapy services was 3.98, especially fee of treatment(4.76), attitude of physical therapist(4.33), and reliance of physical therapist(4.20) were higher by 5-score Likert scale. 3. The correlation factors with general patients' satisfaction were process of treatment (r =.6301). results of treatment(r=. 6186), and guide facilities(r=.5999). 4. The results of multiple-regression analysis between general patients' satisfaction and affecting factors identify following variables as the statically significant determinants : general stisfaction comparing with other physical therapy services, physical therapy services using forwardly, fee of treatment. 5. There was no significant defference between elderly and non-elderly patients' satisfaction of physical therapy service, except attitude of physical therapist(t=-2.07), explanation of treatment for disease(t=-2.19), fee of treatment(t=6.29).

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Age and Growth of Brown Croaker Miichthys miiuy in the South-western Water of Korea (한국 서남해 민어(Miichthys miiuy)의 연령과 성장)

  • Lee, Seung Hwan;Chung, Sang Deok;Yoo, Joon-Taek;Kim, Yeong Hye
    • Korean Journal of Ichthyology
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    • v.29 no.1
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    • pp.69-74
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    • 2017
  • Age and growth of Brown croaker Miichthys miiuy were estimated using right sagittal otoliths of 327 fish specimens from August 2015 to July 2016 in Korea. Annual rings in otoliths were formed from March to June once a year. The oldest specimens were 11 yr in female and 9 yr in male. Brown croaker began spawning in September and showed a peak in October. Total length and body weight relationships showed no significant difference between females and males (P>0.05), and can be expressed as $BW=1.15{\times}10^{-5}{\times}TL^{2928}$ ($R^2=0.98$). Estimated von Bertalanffy growth curve was $L_t=182.4(1-e^{-0.067(t+1.754)})$ ($R^2=0.96$).

DENTAL CARE FORE MULTIPLE ROOTLESS TEETH : A CASE REPORT (다발성 무치근 치아에 대한 치과적 처치)

  • Lee, Mi-Sook;Lee, Keung-Ho;Choi, Yeong-Chul
    • Journal of the korean academy of Pediatric Dentistry
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    • v.28 no.2
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    • pp.316-322
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    • 2001
  • Chemotherapy and radiotherapy used on pediatric oncology patients often causes dentofacial anomalies. Defects noted include tooth and root agenesis, root thinning, root shortening, localized enamel defect and maxillofacial underdevelopment. The effect of radiotherapy usually is confined to the radiation site but the effect of chemotherapy may be more wide spread becuase of its systemic distribution. Many pediatric cancers are treated with a combination of radiation and multiagent chemotherapy. Dental treatment affected by chemotherapy and radiation therapy damage to developing teeth and maxilloface includes retention of teeth, space maintenance, prosthetic considerations, requirements for oral hygiene. The following case related to multiple rootless teeth.

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Development of high performance liquid chromatography assay method of diosmin capsules (디오스민 캡슐의 HPLC 분석법의 개발)

  • Shim, Dae Hyun;Shin, Dong Han;Truong, Quoc Ky;Mai, Xuan Lan;Kang, Jong-Seong;Woo, Mi Hee;Na, Dong-Hee;Chun, In-Koo;Kim, Kyeong Ho
    • Analytical Science and Technology
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    • v.29 no.6
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    • pp.277-282
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    • 2016
  • British Pharmacopoeia (BP 2013), the United States Pharmacopoeia (USP 39) and the Korean Pharmacopoeia (KP XI) contain monographs for the quality control of raw diosmin using high performance liquid chromatography (HPLC). However, official monographs detailing pharmaceutical formulations for diosmin are not available in foreign pharmacopoeias. In the KP XI, ultraviolet-visible (UV-Vis) spectroscopy-which is less specific than HPLC-is reported for the testing of diosmin capsules. In this study, we present an alternative HPLC assay for such testing that is more specific than UV-Vis methods. Method validation was performed to determine linearity, precision, accuracy, system suitability, and robustness. The linearity of calibration curves in the desired concentration range was high ($r^2$>0.999), while the RSDs for intra- and inter-day precision were 0.15-0.29 % and 1.05-1.74%, respectively. Accuracies ranged from 101.2-103.2 %, while the retention time and peak area RSDs were 0.37 % and 0.06 %, respectively. Additionally, the plate number and asymmetry factor values for diosmin were 3591.293 and 1.35, respectively. Since the intermediate-precision and robustness of the assay were satisfactory, this method will be a valuable addition to the Korean Pharmacopoeia (KP XI).

Identification of Marker Nucleotides for the Molecular Authentication of Arisaematis Rhizoma Based on the DNA Barcode Sequences (천남성(天南星) 유전자 감별을 위한 DNA 바코드 분석 및 Marker Nucleotide 발굴)

  • Kim, Wook Jin;Lee, Young Mi;Ji, Yunui;Kang, Young Min;Choi, Goya;Kim, Ho Kyoung;Moon, Byeong Cheol
    • The Korea Journal of Herbology
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    • v.29 no.6
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    • pp.35-43
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    • 2014
  • Objectives : Official Arisaematis Rhizoma is described only three species, Arisaema amurnse, Arisaema erubescens, and Arisaema heterophyllum, in national Pharmacopoeia. However, other Arisaema species, Arisaema ringens, Arisaema takesimense and Arisaema serratum, also have been distributed as an inauthentic Arisaematis Rhizoma in the herbal market. To develop a reliable molecular authentication method for Arisaematis Rhizoma in species level, we analyzed DNA barcode regions using six Arisaema species. Methods : Thirty-eight samples of six Arisaema plants species (A. amurense, A. amurense f. serratum, A. heterophyllum, A. takesimense, and A. serratum) were collected from different habitate and nucleotide sequences of DNA barcode regions (rDNA-ITS, matK, and rbcL gene) were analyzed after PCR amplification. The species-specific sequences and phylogenetic relations were estimated using entire sequences of three DNA barcodes based on the analysis of ClastalW and UPGMA, respectively. Results : The comparative analysis of DNA barcode sequences were revealed inter-species specific nucleotides to distinguish the medicinal plant of Arisaema Rhizoma in species levels excluding between A. amurense and its subspecies (A. amurense f. serratum) and A. takesimense and A. serratum, respectively. However, we obtained sequence differences enough to discriminate authentic and inauthentic Arisaematis Rhizoma. Therefore, we suggest that these SNP type molecular genetic markers were an reliable method avaliable to identify official herbal medicines. Conclusions : These marker nucleotides could be useful to identify the official herbal medicines by providing definitive information that can identify original medicinal plant and distinguish from inauthentic adulterants and substitutes.

Quantitative Determination of Bioactive Compounds in Some Artemisia capillaris by High-Performance Liquid Chromatography

  • Kim, Sang-Won;Kim, Hyeong-Woo;Woo, Mi-Hee;Lee, Jae-Hyun;Choi, Jae-Sue;Min, Byung-Sun
    • Natural Product Sciences
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    • v.16 no.4
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    • pp.233-238
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    • 2010
  • In order to facilitate the quality control of Artemisia capillaris, a simple, accurate and reliable HPLC method was developed for the simultaneous determination of the six bioactive compounds: scopolin (1), chlorogenic acid (2), 2,4-dihydroxy-6-methoxyacetophenone 4-glycoside (3), hyperoside (4), isorhamnetin 3-Orobinobioside (5), and scoparone (6), which were selected as the chemical markers of A. capillaris. Separation was achieved on an Agilent Eclipse XDB-C18 column with a gradient solvent system of 0.1% trifluoroacetic acid aqueous-acetonitrile at a flow-rate of 1.0 mL/min and detected at 254 nm. All six calibration curves showed good linearity ($R^2$ > 0.998). A simple reversed phase HPLC method was developed for extracting pharmacologically active compounds scopolin, chlorogenic acid, 2,4-dihydroxy-6-methoxyacetophenone 4-glycoside, hyperoside, isorhamnetin 3-O-robinobioside, and scoparone from A. capillaris using a binary gradient of acetonitrile : 0.1% trifluoroacetic acid with UV detection at 254 nm. The scopolin (1), chlorogenic acid (2), 2,4-dihydroxy-6-methoxyacetophenone 4-glycoside (3), hyperoside (4), isorhamnetin 3-O-robinobioside (5), and scoparone (6) contents of the herb of A. capillaris collected from fifteen district markets in Korea were 0.00~0.90 mg/g, 0.06~7.29 mg/g, 0.06~0.91 mg/g, 0.07~5.05 mg/g, 0.42~13.11 mg/g, and 1.11~29.82 mg/g, respectively. The results demonstrated that this method is simple and reliable for the quality control of A. capillaris.

Self Care Behavior of Hemodialysis Patients (혈액투석환자의 자가간호행위)

  • Choi, Mi-Kyoung;Choe, Myoung-Ae
    • Journal of Korean Biological Nursing Science
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    • v.9 no.2
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    • pp.105-117
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    • 2007
  • Purpose: The purpose of this study was two folds: first, to identify the level of self care behavior of the hemodialysis patients and second, to find the correlation between the self care behavior and the physiologic indices. Method: The subjects were 52 hemodialysis patients, male and female, who have regularly received hemodialysis dialysis at the Dialysis Room in a leading teaching hospital, Seoul. The patients responded to the self care behavior questionnaires including their socio-demographic characteristics. The respondents have regularly recorded the self care log book. The physiologic indices, clinical characteristics related to the disease and hemodialysis were collected by the chart review. Result: The mean score of the self care behavior was 3.46. The mean score of the self care behavior on categories demonstrated as follows: medication 4.29, fistula management 4.13, management of physical problem 3.71, diet 3.28, exercise and rest 3.22, blood pressure and body weight management 2.97 and social adjustment 2.05 in order. Thirty patients managed discomfort of their fistula. Eleven patients took exercise for 0.5-1 hr/week. Thirty patients measured their body weight daily and thirty two measured their blood pressure daily. The score of self care behavior was significantly correlated with the mean weight gains between the dialysis sessions(r=-.312, p=.05). The mean weight gains between dialysis sessions was found to be high as the level of serum phosphorus and potassium increased(r=-.316, p=.05, r=-.465, p=.01). Conclusion: The result suggests that nursing intervention to the hemodialysis patients to improve self care behavior should be encouraged and further developed.

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Antibiotic Susceptibility of Bacteria Isolated from Infected Root Canals (감염근관에서 분리 배양한 세균의 수종 항생제에 대한 감수성 조사)

  • Lim, Sang-Soo;Kim, Mi-Kwang;Min, Jeong-Beom;Kim, Min-Jung;Park, Soon-Nang;Hwang, Ho-Keel;Kook, Joong-Ki
    • Korean Journal of Microbiology
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    • v.42 no.3
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    • pp.185-194
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    • 2006
  • The aim of this study was to identify the bacteria isolated from endodontic lesions by cell culture and to determine the antimicrobial susceptibility of them against 8 antibiotics. The necrotic pulpal tissues were collected from 27 infected root canals, which were diagnosed as endodontic infection. Samples were collected aseptically from the infected pulpal tissue of the infected root canals using a barbed broach and a paper point. The cut barbed broaches and paper points were transferred to an eppendorf tube containing $500{\mu}l\;of\;1{\times}PBS$. The sample solution was briefly mixed and plated onto a BHI-agar plate containing 5% sheep blood. The agar plates were incubated in a $37^{\circ}C$ anaerobic chamber for 2 to 5 days. The bacteria grown on the agar plates were identified by comparison of 16S rRNA gene (rDNA) sequencing method at the species level. To test the sensitivity of the bacteria isolated from the infected root canals against 8 antibiotics, minimum inhibitory concentrations (MIC) were determined using broth dilution assay. The data showed that 101 bacterial strains were isolated and were identified. Streptococcus spp. (29.7%) and Actinomyces spp. (21.8%) were predominantly isolated. The 9 strains were excluded in antimicrobial susceptibility test because they were lost during the experiment or were not grown in broth culture. The percentage of bacteria susceptible for each antibiotic in this study was clindamycin, 87.0% (80 of 92); tetracycline, 75.0% (69 of 92); cefuroxime axetil, 75.0% (69 of 92); amoxicillin + clavulanic acid (5:1), 71.7% (66 of 92); penicillin G, 66.3% (61 of 92); erythromycin, 66.3% (61 of 92); amoxicillin, 44.6% (41 of 92); and ciprofloxacin, 31.5% (29 of 92). The susceptibility pattern of 8 antibiotics was dependent on the host of the bacteria strains rather than the kinds of bacterial species. These results indicate that antibiotic susceptibility test should be performed when antibiotics are needed for the treatment of infected root canals.

The Crystal and Molecular Structure of the 4-Chloro-4'-Methoxy-2-NitroDi phenylamino $(C_{13}H_{11}N_2O_3CL)$ (4-크로로-4'-메톡시-2-니트로디페닐아민의 X-선 결정 및 분자구조 결정)

  • Nam, Gung-Hae;Yu, Jae-Hyeok;Lee, Hyeon-Mi
    • Korean Journal of Crystallography
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    • v.2 no.2
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    • pp.28-31
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    • 1991
  • 4-크로로-4'-메톡시-2-니트로디페닐아민, (C12H11N2O3CL, FW=278.70)의 단것세포 상수는 a=8,169(3), b=8.883(1), c=9.150(1) h, α =82. 98(1), β=104.80(2), y=101.43(2)", V=627.3 A3, F(000)=288.0, Dc=1.48g/cm3, u=3.06cm-1, 7=295" K, 공간군 Pi, 번호 2, 삼사 정계 이며, Z=2이다. λ (Mo-Ka)=0.7107A을 사용 하여 수집한 독립적인 회절 반점 1541개로 구조분 석한 최종 신뢰도 값은 각각 R=0.032, Rw=0.033 이며, S=0.46이다. 본 화합물은 암모니아의 두개 의 수소 대신에 4-크로로-페닐기와 4-메톡시-페닐기로 치환된 물질로써, 질소와 두 페닐기 사이의 각과 결합거리 들은 각각 125.42", 1.362 및 1.428 A 인바 수소와 함께 SP2_혼성결합을 하고있다. 질소를 포함한 두 면간 각은 63.29"이다. 분자간 어 떠 한 수소결합을 하고 지지 않다.

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Comparision for Hospital P. R. Activities among Large General Hospitals (병원홍보실태에 대한 비교연구)

  • Lee, Hae-Jong;Na, Yu-Mi;Shin, Hee-Young
    • Korea Journal of Hospital Management
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    • v.1 no.1
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    • pp.1-20
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    • 1996
  • This study is purposed to compare PR activities among the 5 large hospitals in Seoul and Kyonggido. The research methods are used in interview survey for employees and Questionnaires survey for patients or their relatives. The 427 patients or their relatives(response rate: 85.4%) were surveyed. The hospital which is below, is more active than any others in special PR activities. 1. Distribution of hospital information books: E, P, A 2. Distribution of calendar: P 3. Broadcast in hospital: E 4. Education of employees about PR : E, A 5. Education of employees about service: all hospitals are active. The PR sectors are organized under the Department of planning(S,J,E) or CEO(P,A). And the budget of PR activities is almost a similar level among all. The degree of recognition and utilization for PR activities is not different among hopitals by the sociodemographic variables of patients. And the average of correlation ratio between recognition of each hopital's PR activities and its utilization is 62.01%(range:51.29%-67.98%) average.

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