• Title/Summary/Keyword: miR-20a

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Quantitative Analysis of Acid Value, Iodine Value and Fatty Acids Content in Vegetable Oils by NIRS (근적외선분광광도법을 이용한 식용유지의 산가, 요오드가, 지방산 정량법에 관한 연구)

  • Kim, Jae-Kwan;Choi, Ok-Kyung;Hwang, Sun-Il;Jeong, Jin-A;Kim, Yun-Sung;Park, Sin-Hee;Son, Mi-Hui;Kwon, Hye-Jung;Lee, Jung-Bock;Kim, Jong-Chan
    • Journal of environmental and Sanitary engineering
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    • v.22 no.4
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    • pp.65-76
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    • 2007
  • The possibility of rapid quantitative analysis of AV(Acid Value), IV(Iodine Value) and fatty acids in vegetable oils with NIRS(Near-infrared spectroscopy) was evaluated. A calibration equation calculated by MPLS regression technique was developed and correlation coefficient of determination for AV, IV, $C_{16:0},\;C_{18:0},\;C_{18:1},\;C_{18:2},\;C_{18:3},\;and\;C_{20:0}$ content were 0.9727, 0.997, 0.9805, 0.942, 0.9987, 0.9994, 0.9966, and 0.975 respectively. According to the data obtained from validation study, $R^2$ of contents of perilla, corn, soybean, rapaseed oils were 0.897, 0.993, 0.935, 0.707, 0.994, 0.996, 0.984, 0.798, SEP of contents of 0.185, 1.367, 0.899, 0.640, 1.498, 1.360, 0.476, 0.076 by MPLS. The results indicate that the NIRS procedure can potentially be used as a non-destructive analysis method for the rapid and simple measurement of AV, IV and fatty acids in vegetable oils.

Preparation and Characterization of Microporous PVdF Membrane for Li-ion Rechargeable Battery (이차전지용 미세다공성 PVdF 분리막의 제조와 물성)

  • Nam, Sang-Yong;Yu, Dae-Hyun;Jeong, Mi-Ae;Rhim, Ji-Won;Byun, Hong-Sik;Jeong, Chul-Ho;Lee, Young-Moo;Seo, Myung-Su
    • Membrane Journal
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    • v.17 no.3
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    • pp.233-243
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    • 2007
  • In this study, a separate. which is a microporous membrane based on poly(vinylidene fluoride)(PVdF) was prepared by phase inversion method. Being prepared by dissolving the PVdF in the N,N'-dimethylformamide(DMF) with mechanical stirring, the homogenous casting solution was cast onto a clean glass plate. Pore size and porosity of the membranes were controlled by changing preparation condition. The highest porosity of the membrane was 78.6%. The mechanical property of the membrane was determined by using an universal testing machine(UTM). The morphology of the membrane was investigated by scanning electron microscopy(SEM). The cross-section of the membrane shows sponge-like small micro-pores.

Development of Controlled Release Oral Drug Delivery System by Membrane-Coating Method-II-Correlation Between Acetaminophen Concentrations in Plasma and Sativa Samples of Man- (피막법에 의한 경구투여용 제어방출 제제의 개발-II-사람에 있어서 아세트아미노겐 혈장 중 농도와 타액 중 농도와의 상관성-)

  • Shim, Chang-Koo;Kim, Mi-Ae;Lee, Min-Hwa;Kim, Shin-Keun
    • Journal of Pharmaceutical Investigation
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    • v.20 no.1
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    • pp.29-33
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    • 1990
  • Plasma and saliva concentrations of acetaminophen (AAP) were determined at various time points by HPLC after oral administration of AAP tablets (AAP 500 mg) to four healthy male Korean subjects. Saliva concentrations (S) of AAP were significantly correlated with plasma AAP concentrations (P). The S/P ratio of AAP was calculated to be 1.05 (r =0.944, $p<10^{-6}$) for all the data points from the subjects. It showed a little intersubject variation and ranged from 0.89 to 1.46 in each subject. Bioavailability parameters such as AUC, $C_{max}$ and $T_{max}$ which are usually obtained from the plasma concentration data will be predictable approximately by saliva concentration data. Saliva seems to be very convinient and useful samples for the preliminary studies of bioavailability and bioequivalence of AAP preparations, since it can be collected frequently without any painful venipuncture to the subjects, that is inevitable in plasma sampling. Evaluation of the bioavailability of a preparation by saliva samples will reduce the cost, time and safety risk greatly in developing a new drug delivery system for AAP.

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Evaluation of Various PCR Assays for Detection of Emetic-Toxin-Producing Bacillus cereus

  • Kim, Jung-Beom;Kim, Jae-Myung;Park, Yong-Bae;Han, Jeong-A;Lee, Soon-Ho;Kwak, Hyo-Sun;Hwang, In-Gyun;Yoon, Mi-Hye;Lee, Jong-Bok;Oh, Deog-Hwan
    • Journal of Microbiology and Biotechnology
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    • v.20 no.7
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    • pp.1107-1113
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    • 2010
  • Because conventional methods for detecting emetic-toxin-producing B. cereus are laborious and costly, various PCR assays, which are easy and cheap, have recently been reported. Therefore, this study estimated and compared the ability of various PCR assays to detect emetic-toxin-producing B. cereus strains isolated in Korea. The PCR assays were performed on 160 B. cereus strains, including 40 emetic-toxin-producing strains. Although the species-specific PCR assays were all shown to be highly specific, the sensitivities varied greatly. The accuracies of the primers were 97.5% (CER), 95.6% (EM1), 96.3% (RE234), 89.4% (CES), and 83.1% (Ces3R/CESR2). Moreover, the CER primer had a higher sensitivity (100%) than all the other primers tested, and a specificity of 96.7%. Thus, the CER primer was shown to be the most effective for screening the emetic-toxin-producing B. cereus strains tested in this study. However, the ability of these PCR assays to identify emetic-toxin-producing B. cereus should also be confirmed using other methods.

Processing of Vinegar Using Gyoungok-go (경옥고를 이용한 식초의 개발)

  • Park, Sung-Min;Lee, Won-Wook;Jo, Mi-Jeong;Lee, Jong-Rok;Jung, Dae-Hwa;Park, Eun-Young;Byun, Sung-Hui;Kim, Sang-Chan
    • Herbal Formula Science
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    • v.17 no.1
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    • pp.121-128
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    • 2009
  • Gyoungok-go (GOG; Qiongyu-gao in Chinese) has been used in traditional oriental medicine for the improvement of various physiological vitality functions. The aim of present work is to investigate a processing possibility of vinegar using GOG. GOG vinegar was prepared by two stage fermentations of alcohol and vinegar. The method of GOG fermentation with polished rice as a fermentation substrate is not yet established. The result of alcohol fermentation rate of GOG with some strains, strain No. 1 produced the highest alcohol contents among 7 strains. The strain No. 1 identified Saccharomyces paradoxus by 18S rDNA classification. S. paradoxus was produced over 20% alcohol in the 1$\sim$11% GOG with polished rice. A. aceti was produced 8.52% acetic acid from 6.5% alcohol containing 11% GOG, after incubation for 11 days.

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Contrasting rice sub-populations to tocols ratio associated with seed longevity

  • Lee, Jae-Sung;Kwak, Jieun;Yoon, Mi-Ra;Lee, Jeom-Sig;Hay, Fiona R.
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.31-31
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    • 2017
  • Understanding the mechanism(s) to overcome or prevent seed ageing deterioration during storage is of fundamental interest to seed physiologists. Vitamin E (tocols) is known as a key metabolite to efficiently scavenge lipid peroxy radicals which cause membrane breakdown resulting in seed ageing. However, in rice research this hypothesis has been tested for very few lines only without considering intraspecific variation in genomic structure. Here, we present a correlation study between tocols and seed longevity using a diverse rice panel. Seeds of 20 rice accessions held in the International Rice Genebank at the International Rice Research Institute, representing aus, indica, temperate japonica and tropical japonica subpopulations, were used for tocols analysis (quantification of ${\alpha}$-, ${\beta}$-, ${\gamma}$-, ${\delta}$-tocopherol/tocotrienol by ultra performance liquid chromatography) and storage experiments at $45^{\circ}C$ and 10.9% seed moisture content (sample taken for germination testing every 3 days up to 60 days). To examine interactions between DNA sequences and phenotype, the 700k high-density single-nucleotide polymorphism marker data-set was utilized. Both seed longevity (time for viability to fall to 50%; $p_{50}$) and tocols content varied across subpopulations due to heterogeneity in the genetic architecture. Among eight types of tocol homologues, ${\alpha}$-tocopherol and ${\gamma}$-tocotrienol were significantly correlated with $p_{50}$ (negatively and positively, respectively). While temperate japonica varieties were most abundant in ${\alpha}$-tocopherol, indica varieties recorded 1.3 to 1.7-fold higher ${\gamma}$-tocotrienol than those of other subpopulations. It was highlighted that specific ratio of tocol homologues rather than total tocols content plays an important role in the seed longevity mechanism.

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Biological Resources Potential and the Recent State of International Cooperation in Indonesia

  • Park, Chang-Ho;Junaedi, Ahmad;Lee, Mi-Ra;Lee, Yeon-Hee
    • Interdisciplinary Bio Central
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    • v.2 no.4
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    • pp.11.1-11.8
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    • 2010
  • Indonesia is a mega-biodiversity country having at least one trillion US Dollars value of biological resources per year over the next 20 years. Indonesia is home to 11 percent of the world's flowering plant species and 12 percent of all mammals. Many of Indonesia's species and more than half of the archipelago's endemic plant species are found nowhere else on the Earth. This information is just a small portion of all higher plants and animals being found in Indonesia. Former studies described that total number of species in Indonesia is estimated more than 1,000,000 and most of them remain unknown scientifically. Most of lower plants and animals have not been studied yet, so that greatest portion of Indonesia's biological resources has never been assessed with respect to its economic value or conservation status. Many developed countries are building cooperation with Indonesia on resources, mainly in the fields of grant aid, socio-economic services, R & D, researcher exchange, technology transfer, infrastructure, education/training, finance, etc. Indonesia will obtain greater benefits and management of its biological richness via increasing its international capacity to add value and information to its biological diversity. These goals can be achieved by close international collaboration on search of important biological resources and other bioactive products that have potential economic values. Development of biological resource-based technology stands as the industry of the $21^{st}$ century and, therefore, Indonesia has a unique opportunity to lead the process in the world.

Quality Characteristics of Naengmyeon Noodle Containing Citric Acid and Guar Gum (구연산과 구아검 첨가 냉면의 품질특성)

  • Park, Jae-Hee;Ryu, Bog-Mi;Kim, Chang-Soon
    • Korean journal of food and cookery science
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    • v.32 no.4
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    • pp.426-432
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    • 2016
  • Purpose: The consumption of noodles has increased domestically. However, noodles with high carbohydrate content can cause an increase in blood glucose compared with other foods. Therefore, in this study, Naengmyeon with high resistant starch was prepared for decreasing blood glucose by the addition of 0.5% citric acid (CN), 1% guar gum (GN) or 0.5% citric acid and 1% guar gum (CGN), and then it was incubated in a refrigerator for 3 days, and stored in a freezer for 1 month. Methods: The quality characteristics of these Naengmyeon noodles was evaluated based on total starch, resistant starch, water absorption, cooking loss, turbidity, in vitro starch hydrolysis, and in vivo glucose response. Results: There was no significant difference in the total starch, cooking loss, and turbidity. The resistant starch of GN (1.70%) and CGN (1.84%) was significantly increased when compared with that in Naengmyeon with no additives (N) and CN. In terms of water absorption, CN (86.01%) was the lowest in samples, followed by GN (92.17%), N (94.20%), and CGN (99.16%). CGN with high resistant starch was the lowest in in vitro starch hydrolysis in samples. However, it had no effect on the in vivo glucose response. In vitro starch hydrolysis exhibited a significant positive correlation (r=0.533; p<0.01) with in vivo glucose response. Conclusion: Therefore, future studies are needed to establish the standard for resistant starch contents in processed carbohydrate foods for delaying the increase in blood glucose. If this standard is established, it might help to develop processed foods for diabetic patients.

DEPRESSION IN CHILDREN - AN OVERVIEW AND THE DEVELOPMENTAL PERSPECTIVES ON CHILDREN IN DEPRESSION - (소아기 우울증 - 소아기 우울증의 개관과 발달학적 측면 -)

  • Kang, Kyung-Mi
    • Journal of the Korean Academy of Child and Adolescent Psychiatry
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    • v.5 no.1
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    • pp.3-11
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    • 1994
  • Childhood depression was not officially accepted as a separate diagnosis until 20-25years ago. Although DSM-III R criteria gave us a starting point and are gaining acceptances as a useful tool for diagnosing childhood depression recently, many professionals feel that additional modifications are needed to better reflect the developmental aspects of children. And because children are relatively poor in expressing their moods or feelings due to weak reality sence and/or cognitive and linguistic limitations, the author too carefully suggest that developmental perspectives of children should be considered in diagnosing childhood depression. Little is known about the extent to which childhood depression precedes adult depression and persists into adult life and about the impact of it on the personality formation. Some evidences that childhood depressive symtoms can be quite enduring make it important to make an early diagnosis and treatment.

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The Influence of Post-operative Discomfort, Sense of Loss, and Family Support on Resilience in Patients after Breast Cancer Surgery (유방암 수술환자의 수술 후 불편감, 상실감, 가족지지가 회복력에 미치는 영향)

  • Kwan, An Na;Kim, Tae Hyun;Lee, Yun Mi
    • Journal of Korean Critical Care Nursing
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    • v.10 no.2
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    • pp.34-44
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    • 2017
  • Purpose: The purpose of this study was to identify post-operative discomfort, sense of loss, family support, and resilience in breast cancer surgery patients, and to investigate factors that affect resilience. Method: The sample of this study consisted of 108 patients who underwent surgery for breast cancer in two university hospitals located in B city. The collected data was analyzed with descriptive statistics, t-test, ANOVA, and Scheffé test, Pearson's correlation coefficients, and hierarchical multiple regression. Results: The factors that significantly affected resilience were as follows: having a religion (${\beta}=-.20$, p=.006), having an occupation (${\beta}=.14$, p=.049), having a high school diploma (${\beta}=.31$ p=.001), making less than 2-3 million won (${\beta}=-.19$, p=.036) per month, experiencing a sense of loss (${\beta}=-.22$, p=.003) and family support (${\beta}=.44$, p<.001). The total explanatory power amounted to 53.8% (F=14.83, p<.001, $AdjR^2=.54$). Conclusion: Educational intervention programs for breast cancer surgery patients that improve resilience by reducing the sense of loss and increasing family support must be developed.

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