• BMB Reports
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• 제56권3호
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• pp.190-195
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• 2023

We propose a novel blood biomarker detection method that uses miRNA super-resolution imaging to enable the early diagnosis of Alzheimer's disease (AD). Here, we report a single-molecule detection method for visualizing disease-specific miRNA in tissue from an AD mice model, and peripheral blood mononuclear cells (PBMCs) from AD patients. Using optimized Magnified Analysis of Proteome (MAPs), we confirmed that five miRNAs contribute to neurodegenerative disease in the brain hippocampi of 5XFAD and wild-type mice. We also assessed PBMCs isolated from the whole blood of AD patients and a healthy control group, and subsequently analyzed those samples using miRNA super-resolution imaging. We detected more miR-200a-3p expression in the cornu ammonis 1 and dentate gyrus regions of 3 month-old 5XFAD mice than in wild-type mice. Additionally, miRNA super-resolution imaging of blood provides AD diagnosis platform for studying miRNA regulation inside cells at the single molecule level. Our results present a potential liquid biopsy method that could improve the diagnosis of early stage AD and other diseases.

• Food Science and Biotechnology
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• 제17권5호
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• pp.899-903
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• 2008

This study was conducted in order to elucidate the optimum conditions for the extraction of clove that can be used to elicit antibacterial activity against Streptococcus mutans using the evolutionary operation (EVOP)-factorial design technique. Higher antibacterial activity was achieved in a higher extraction temperature of $80^{\circ}C$ ($r=0.7983^{**}$) and in a longer extraction time of 26 hr ($r=0.6867^*$). Antibacterial activity was not effected by differentiation of ethanol concentration in the extraction solvent (r=-0.0683). The maximum antibacterial activity of clove against S. mutans as determined by the EVOP-factorial design technique was obtained at an extraction temperature of $80^{\circ}C$, an extraction time of 26 hr and a 50% ethanol concentration. Furthermore, the population of S. mutans decreased from an initial concentration of 6.850 to 4.195 log CFU/mL in the third set that is more than 2.6 log cycles by EVOP-factorial design technique.

• Toxicological Research
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• 제29권3호
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• pp.195-201
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• 2013

The anti-inflammatory effects of glycosaminoglycan (GAG) derived from Isaria sinclairii (IS) and of IS extracts were investigated in a complete Freund's adjuvant (CFA)-treated chronic arthritis rat model. Groups of rats were treated orally with 30 mg/kg one of the following: [1] saline control, extracts of [2] water-IS, [3] methanol-IS, [4] butanol-IS, [5] ethyl acetate-IS, or [6] Indomethacin(R) as the positive control for a period of two weeks. The anti-paw edema effects of the individual extracts were in the following order: water-IS ex. > methanol ex. > butanol ex. > ethyl acetate ex. The water/methanol extract from I. sinclairii remarkably inhibited UV-mediated upregulation of NF-${\kappa}B$ activity in transfected HaCaT cells. GAG as a water-soluble alcohol precipitated fraction also produced a noticeable anti-edema effect. This GAG also inhibited the pro-inflammatory cytokine levels of prostaglandin $E_2$-stimulated lipopolysaccharide in LAW 264.7 cells, cytokine TNF-${\alpha}$ production in splenocytes, and atherogenesis cytokine levels of vascular endothelial growth factor (VEGF) production in HUVEC cells in a dose-dependent manner. In the histological analysis, the LV dorsal root ganglion, including the articular cartilage, and linked to the paw-treated IS GAG, was repaired against CFA-induced cartilage destruction. Combined treatment with Indomethacin(R) (5 mg/kg) and IS GAG (10 mg/kg) also more effectively inhibited CFA-induced paw edema at 3 hr, 24 hr, and 48 hr to levels comparable to the anti-inflammatory drug, indomethacin. Thus, the IS GAG described here holds great promise as an anti-inflammatory drug in the future.

• 산업융합연구
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• 제21권10호
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• pp.151-158
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• 2023

본 연구는 간호대학생을 대상으로 전공만족도, 학습열의가 핵심간호술 수행자신감에 미치는 효과를 확인하기 위해 시도되었다. J도의 간호대학생을 대상으로 하였으며 112명의 설문지를 분석에 사용하였다. 일반적 특성에 따른 핵심간호술 수행자신감은 연령(F=5.52, p<.001), 간호사 직업 이미지(F=10.2, p<.001)에서 유의한 차이를 나타냈다. 대상자의 전공만족도는 핵심간호술 수행자신감(r=.563, <.001)과 정적 상관 관계를 나타냈으며, 간호대학생의 핵심간호술 수행자신감에 영향을 미치는 요인은 전공만족도(𝛽=.338 P<.001), 학습열의(𝛽=.195 P=.027)로 확인되었고, 변인들에 의한 설명력은 35%였다. 따라서 본 연구의 결과는 간호대학생의 핵심간호술 수행 능력 향상을 위한 교육 개발에 중요한 기초 자료가 될 것이다.

• 한국산학기술학회논문지
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• 제17권6호
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• pp.326-337
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• 2016

본 연구의 목적은 병원급별 간호사들의 감성지능과 직무만족, 간호서비스 질에 대한 상관관계를 확인하여 간호인력 관리와 간호서비스 질을 향상하기 위한 기초자료로 활용하기 위하여 시행되었다. 본 연구는 G도에 위치한 상급종합병원 1곳과 종합병원 2곳에서 일반간호사 195명을 대상으로 2014년 4월 7일부터 4월 28일까지 자료수집 하였으며, 수집된 자료는 SPSS 18.0 프로그램을 이용하여 분석하였다. 상급종합병원과 종합병원에서 감성지능과 간호서비스 질이 각각 높은 정적 상관관계가 있는 것으로 나타났다(r=.624, p<.001, r=.612, p<.001). 상급종합병원의 경우 감성지능과 직무만족이 정적 상관관계로 나타났다(r=.430, p<.001). 본 연구 결과를 바탕으로 상급종합병원과 종합병원 모두 간호사의 감성지능과 직무만족을 증진시킴으로써 간호서비스 질을 높일 수 있음을 확인하였다. 특히 감성지능과 간호서비스 질 간의 높은 관련성으로 볼 때 상급종합병원과 종합병원의 특성에 맞게 간호사의 감성지능을 강화시킬 수 있는 업무 환경 조성 및 감성지능 향상 교육프로그램을 개발하여 제공하는 것이 필요하며, 연령 및 경력이 낮은 간호사를 중심으로 감성지능을 높일 수 있는 적극적인 방안이 요구된다.

• 대한간호학회지
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• 제35권1호
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• pp.195-205
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• 2005

Purpose: This study was to identify the relationship between perceived stress, anger expression, and level of depression in cancer patients. Method: A cross-sectional descriptive study design was used. Data was collected by uestionnaires from 185 in- and out-patients who were diagnosed with cancer at 3 university hospitals and the National Cancer Center using Spielberger et al.’s Anger Expression Scale, Cohen, Kamarch & Mermelstein's Perceived Stress, and Derogatise's SCL-90. The data was analyzed using descriptive statistics, Pearson correlation coefficient, and stepwise multiple regression with SAS/PC. Result: The perceived stress in cancer patients indicated a significant positive correlation to anger-in(r=.288, p=.000), anger-out(r=.232, p=.001), and depression(r=.68, p=.000), but no significant correlation to anger-control. The anger-in of cancer patients showed a significant positive relationship to anger-out(r=.53, p=.000), and depression(r=.383, p=.000), but no significant correlation to anger-control. Anger-out showed a significantly negative correlation to anger-control (r=-.248, p=.001) and a positive correlation to depression (r=.240, p=.001). The most significant predictor which influenced depression in cancer patients was perceived stress, followed by anger-in and hobby, and these factors explained their depression with a variance of 54%. Conclusion: These results suggested that cancer patients with a high degree of perceived stress are likely to be high in anger-out and anger-in. Perceived stress and anger-in are major factors which affect depression in cancer patients.

• 한국재난정보학회:학술대회논문집
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• 한국재난정보학회 2023년 정기학술대회 논문집
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• pp.195-197
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• 2023

본 연구는 재난에서 사용되고 있는 정보통신기술( ICT) 연구 국내논문 100개 및 해외논문 100개를 수집한 후, R 프로그램을 이용한 텍스트 마이닝 분석을 통해 국내 재난관리 네 가지 단계에 따른 ICT 기술 관련 연구 동향을 파악하고, 해외 재난관리의 ICT 기술 연구 동향을 분석 활용하여 우리나라 재난에서의 ICT 기술 활용방안을 새롭게 구성해 제언하는 것이다.

• 보건의료산업학회지
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• 제6권3호
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• pp.183-195
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• 2012

The purpose of this study was to examine the level of stress, somatization, anger, adjustment to school according to the types of housing. This study employed a descriptive design. Data was collected from 552 students in a girls high school in gongju using structured instruments. Not to be influenced by the tension of new school year or the stress by taking tests, the researcher did the survey after students finishing midterm test of the first semester for two days from July 14 to 15. The results are as follows. There was no difference in the level of stress, anger, adjustment to school between the students living in a dormitory and the students not living in a dormitory. However there was remarkable difference in somatization. There was positive correlation between somatization and stress(r=.194, p=.011), between anger and stress(r=.463, p<.001), in contrast there was a negative correlation between adjustment to school and stress(r=-.174, p<.001) of students living in a dormitory. On the other hand, there was negative correlation stress(r=-.187, p<.001), somatization(r=-.252, p<.001), anger(r=-.230, p<.001) with adjustment to school of students not living in a dormitory. In the sub-factors of somatization, students who live in a dormitory have many kinds of somatizations of digestive or respiratory organs. A Health promotion program should be designed for girls high school students living in a dormitory, based on the level of somatization of digestive or respiratory organs.

• 근관절건강학회지
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• 제24권1호
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• pp.14-23
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• 2017

Purpose: The purpose of this study was to identify the factors affecting the perceptions of patient safety culture, safety care knowledge, and safety care activity among nurses at orthopedic hospitals. Methods: Data were collected during Feb. 16 and Feb. 26, 2017, from 195 nurses of 9 small to medium sized orthopedic hospitals. Questionnaires about patient safety culture, safety care knowledge and activity were used. Data were analyzed by descriptive statistics, t-test, ANOVA, $Scheff\acute{e}$ test, Pearson's correlation coefficient, and stepwise multiple regression. Results: The safety care activity was positively correlated with perceptions of patient safety culture (r=.50, p<.001) and knowledge (r=.48, p<.001). Factors that had influence on the safety care activity were the patient safety culture (${\beta}=.30$, p<.001), age (${\beta}=.27$, p<.001), and knowledge of the safety activity (${\beta}=.21$, p=.004). The patient safety care activity was explained 36.6% by those factors. Conclusion: To enhance the patient safety care activity, it should be provided the environment and open communication for the perceptions of patient safety culture and the in service education program for safety care knowledge.

• 재활간호학회지
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• 제3권2호
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• pp.181-195
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• 2000

The purpose of this study is to find out the effects of personal characteristics, social support, depression, and metamemory on the older adults' memory performances. The subjects of the study consisted of 95 older adults over the age of 60 who are living in Busan. Some data were collected by means of the interview method, using questionnaires for metamemory (MIA questionnaire by Dixon, et al., 1988), and depression(GDS by Yesavage and Sheikl, 1986), social resoueces(Duke university, 1978), family support(Hyun-Sook Kang, 1985). The other data were collected by the testing method on the memory performance such as the immeadiate word recall task, the delayed word recall task, the word recognition task(Elderly Verbal Learning Test by Kyung Mi Choi, 1998), and the face recognition task(Face Recognition Task tool developed by Hye-Sook Min). The results of this study were as follows; 1. The level of metamemory is 3.4 points in the 5 point scale, the grades of the task and the achievement are relatively high and the grades of the change, the control, and the anxiety are relatively low in the sub-concepts. 2. Metamemory have significant relation with age(r=-.44), educational attainment(r=.46), depression(r=-.58), family support(r=.20), social resources(r=.20). 3. The significant variables to predict older adults' metamemory are educational attainment(22%), sex(11%), age (8.3%), depression(4.7%), and illness state(3%). 4. The strong variables to predict memory performances are metamemory, age, depression, social resources, educational attainment, illness state, and limitation of daily living activity related to illness. In conclusion, the enhancement strategies of metamemory and the social support and the prevention or reduction of depression are necessary to increase older adults' memory performances. Ultimately in this respect nurses' roles are very important in developing and performing some intervention programs for old adults' memory improvement, which have significant meanings in the field of nursing science.