• Title/Summary/Keyword: miR-128

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On the Breakdown Voltage of Spherical PN Junction (구형 PN 접합의 항복전압에 대한 소고)

  • Kim, Hae-Mi;Yun, Jun-Ho;Choi, Yearn-Ik;Jo, Jung-Yol
    • Proceedings of the KIEE Conference
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    • 2008.10a
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    • pp.127-128
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    • 2008
  • Baliga는 구형 PN 접합에 대한 해석적인 항복전압을 유도하였으며, 이 식은 ${\gamma}(r_j/W_{pp})$ 값이 1보다 큰 구간에서 구형 접합의 항복전압이 원통형 및 평면형 항복진압 보다 큰 값을 가지게 된다. 기본적으로 구형 접합의 한복전압이 원통형과 평면형 접합의 항복전압보다 클 수 없기 때문에 이는 명백한 오류이다. 이 오류는 식을 구하는 과정에서 소수점 두 자리를 무시하고 계산을 하였기 때문임을 밝혀냈으며, 또한 이러한 작은 실수가 구형 접합의 항복전압에 미치는 영향이 지대할 수 있다는 결과를 보고하고자 한다.

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The Dementia Knowledge, Attitude and Preventive behaviors of Hemodialysis patients (혈액투석 환자의 치매에 대한 지식, 태도 및 예방행위)

  • Seo, Eun-Koung;Lee, Mi-Ra
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.19 no.12
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    • pp.795-804
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    • 2018
  • The purpose of this study was to identify the dementia knowledge, attitudes and preventive behaviors of hemodialysis patients. The participants were 128 hemodialysis patients at the Artificial Kidney Center in G hospital of P city. Data were collected by self-report questionnaires from September 22 to October 10, 2017. Data were analyzed with descriptive statistics, t-test, ANOVA, Pearson's correlation coefficient and Hierarchical regression analysis with SPSS/WIN 23.0. Dementia knowledge was significantly different by education level (F=4.58, p<.01). The attitude toward dementia was different by age (F=3.08, p<.05) and education level (F=6.64, p<.01). The preventive behaviors were different by gender (t=-2.30, p<.05). Dementia knowledge was positively correlated with attitude toward to dementia (r=.189, p<.05) and attitude toward dementia was positively correlated with preventive behaviors (r=.280, p<.01). The influencing factor for preventive behaviors was attitude toward dementia (${\beta}=.319$, p<.01). Therefore, in order to promote dementia preventive behaviors in hemodialysis patients, an education program should be developed to improve dementia knowledge and positive attitude toward dementia.

Molecular Cloning and Characterization of a Flavanone-3-hydroxylase Gene from Rubus occidentalis L.

  • Lee, Seung Sik;Lee, Eun Mi;An, Byung Chull;Barampuram, Shyamkumar;Kim, Jae-Sung;Cho, Jae-Young;Lee, In-Chul;Chung, Byung Yeoup
    • Journal of Radiation Industry
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    • v.2 no.3
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    • pp.121-128
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    • 2008
  • Flavanone-3-hydroxylase (F3H) is one of the key enzymes for the biosynthesis of flavonals, anthocyanins, catechins and proanthocyanins. F3H catalyzes the $3{\beta}$-hydroxylation of (2S)-flavonones to form (2R, 3R)-dihydroflavonols. In this report, we isolated a full-length cDNA of RocF3H from black raspberry (Rubus occidentalis L.) using a reverse transcriptase-PCR and rapid amplification of the cDNA ends (RACE)-PCR. The full-length cDNA of RocF3H contains a 1,098 bp open reading frame (ORF) encoding a 365 amino acid protein with a calculated molecular weight of about 41.1 kDa and isoelectric point (pI) of 5.45. The genomic DNA analysis revealed that the RocF3H gene had three exons and two introns. Comparison of the deduced amino acid sequence of the RocF3H with other F3Hs revealed that the protein is highly homologous with various plant species. The conserved amino acids ligating the ferrous iron and the residues participating in the 2-oxoglutarate binding (R-X-S) were found in RocF3H at the similar positions to other F3Hs. Southern blot analysis indicated that RocF3H exist a multi-gene family. The isolation of RocF3H gene will be helpful to further study the role of F3H gene in the biosynthesis of flavonoids in R. occidnetalis.

A Study on Self-Esteem, Health Promoting Behavior and the Quality of Life of the patients undergoing Hemodialysis (혈액투석환자의 자아존중감, 건강증진행위와 삶의 질과의 관계연구)

  • Chun, Chung Ja;Jung, Young Mi;Cho, Hyun Min;Kim, Jung Sik;Paek, Ok Hee;Kim, Jung Hee;Park, Jin Hee
    • Korean Journal of Adult Nursing
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    • v.12 no.1
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    • pp.134-146
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    • 2000
  • This study was conducted to investigate the relationship between self-esteem, health promoting behavior and the quality of life of the patients undergoing hemodialysis. The subjects were 86 patients undergoing hemodialysis in H hospital. The data were collected using a questionnaire. Data collection was done from January 11th to June 16th, 1999. The analysis of data was done by use of descriptive statistics, t-test, ANOVA, Pearson Correlation Coefficient and Multiple regression using the SPSS/PC+ program. The results are as follows: 1. The mean of the level of self-esteem was 25.02, the level of health promoting behavior, 120.04 and the level of QOL, 128.09. 2. Test for hypothesis: Hypothesis 1. "The higher the level of self-esteem of the patients undergoing hemodialysis, the higher the level of quality of life will be." was supported(r=.296, p<.01). Hypothesis 2. "The higher the level of health promoting behavior, the higher the level of quality of life will be." was supported (r=.628, p<.001). 3. Health promoting behavior explained 39.5percent of QOL($R^2$=.395, F=27.040, p<.001). 4. Monthly income(F=3.85, p<.01) and marital status(F=4.64, p<.05) were significantly related to the quality of life In conclusion, this study showed that self-esteem and health promoting behavior may be important factors that can improve the quality of life of the patients undergoing hemodiaysis. This study identified that nursing plans should include these factors to help physical, psychological and social adaptation of the patients undergoing hemodialysis.

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Mutation in gyrA gene of nalidixic acid-resistant Salmonella isolates isolated from poultry slaughterhouse (닭 도축장에서 분리한 nalidixic acid 내성 Salmonella 균의 gyrA 유전자 돌연변이)

  • Cho, Jae-Keun;Son, Kyu-Hee;Kim, Kyung-Hee;Kim, Jeong-Mi;Park, Dae-Hyun;Lee, Jung-Woo
    • Korean Journal of Veterinary Service
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    • v.42 no.3
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    • pp.153-159
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    • 2019
  • The objective of this study was to identify mutations in the quinolone resistance determining region (QRDR) of the gyrA, gyrB, parC and parE genes, and the presence of plasmid-mediated quinolone resistance (PMQR) genes: qnrA, qnrB, qnrS, aac(6')-lb-cr and qepA in 40 nalidixic acid- resistant ($NA^R$) Salmonella isolates isolated from poultry slaughterhouse. The MIC of NA and ciprofloxacin for 40 $NA^R$ Salmonella isolates was $128{\sim}512{\mu}g/mL$ and < $0.125{\sim}0.25{\mu}g/mL$, respectively. The Salmonella isolates were resistant to NA (100%), gentamicin (5.0%) and ampicillin (2.5%). All $NA^R$ Salmonella isolates represented point mutation in codons Aspartic acid(Asp)-87 (90%) and Serine(Ser)-83 (10%) of QRDR of gyrA gene: $Asp87{\rightarrow}glycine$, $Ser83{\rightarrow}tyrosine$. No mutations were observed in QRDR of the gyrB, parC and parE gene. Moreover PMQR genes was not found in any of the tested isolates. Our findings showed that DNA gyrase is the primary target of quinolone resistance and a single mutation in codon Asp87 and Ser83 of the gyrA gene can confer resistance to NA and reduced susceptibility ciprofloxacin in Salmonella isolates.

Clinical Analysis Of Ventricular Septal Defect (심실중격결손증의 외과적 고찰)

  • Seong, Suk-Hwan;Suh, Kyung-Pill
    • Journal of Chest Surgery
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    • v.15 no.1
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    • pp.90-97
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    • 1982
  • Two hundred one patients of ventricular septal defect, which were operated at Seoul National University Hospital, were analysed on clinical background during the period from January, 1975 to December, 1980. The results were as follows: 1. Of the 201 patients, 118 patients were male [58.7%] and 83 patients were female [41.3%]. Their age ranged from 15 months to 40 years, and the mean age was 8.7 years. 40% of the patients were between 4 and 8 years. 2. The most common symptoms showed frequent U RI and exertional dyspnea. 3. On Kirklin`s anatomical classification, type I constituted 26.9%, type II 58.2%, type III 12.4%, and type IV 1.0%. We showed marked increased incidence of type I VSD as compared to Caucasians* 4. 46 cases were associated with other congenital cardiac diseases. They were PDA [13 cases], AI[11 ], ASD[6], PS[10], MI[4], and Double aortic arch [1]. 5. In 128 patients, who had complete hemodynamic data and were not associated with other congenital cardiac diseases, an attempt was made to correlate the EKG findings with the hemodynamic data, and defect size with the hemodynamic data. The children had variable distribution of PA syst. pr. and Rp/Rs. But most of adults had $R_P$/$R_S$of 0.15 or less. As $P_P$/$P_S$increased, the rate of operative complication increased also. 6. When a normal EKG pattern was present, $Q_P$/$Q_S$and $R_P$/$R_S$and $P_P$/$P_S$were relatively low. When EKG findings were LVH pattern, there was diastolic volume overload to left ventricle. As RVH, there was systolic pressure overload to right ventricle. And as BVH, there was mixed pattern of diastolic volume overload to left ventricle and systolic pressure overload to right ventricle. 7. Among patients in defect was less than 1 $cm^2$ per $M^2$ of BSA, $Q_P$/$Q_S$was less than 2:1, and $R_P$/$R_S$less than 0.25, and PAsyst. pr. less than 50 mmHg, and $P_P$/$P_S$was less than 0.5. But patients with the defect greater than 1 $cm^2$ per $M^2$ of BSA had no correlationship between $Q_P$/$Q_S$, $R_P$/$R_S$, PAsyst. pr. and defect size in each other. Most of patients with the defect greater than 2 $cm^2$/$M^2$ BSA, $R_P$/$R_S$was greater than 0.5. 8. Operative mortality rate was 9.5% [19 cases] among 201 patients. And complication rate including mortality rate was 22.9% [46 cases].

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Antioxidative and Cellular Protective Effects of Dolwoe (Gynostemma pentaphyllum Makino) Extracts against Oxidative Stress (돌외(Gynostemma pentaphyllum Makino) 추출물의 산화적 스트레스에 대한 항산화 및 세포보호효과)

  • Kim, Kyoung Mi;Kim, A-Rang;Kim, A-Young;Ha, Ji Hun;Xuan, Song Hua;Jeong, Yoon Ju;Park, Young Min;Jeong, Hyo Jin;Hong, In Gi;Park, Soo Nam
    • Korean Journal of Pharmacognosy
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    • v.48 no.2
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    • pp.125-133
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    • 2017
  • In this study, we investigated the total phenolic and flavonoid contents, component analysis, antioxidative activity and cellular protective effects against oxidative stress on human skin cells in 50% ethanol extract, ethyl acetate fraction and aglycone fraction obtained from Gynostemma pentaphyllum (G. pentaphyllum) Makino. The DPPH (1,1-diphenyl-2-picrylhydrazyl) scavenging activites ($FSC_{50}$) of the 50% ethanol extracts, ethyl acetate fraction and aglycone fraction were 246.8, 147.2, $128.9{\mu}g/mL$, respectively. Reactive oxygen species (ROS) scavenging activities ($OSC_{50}$) of the 50% ethanol extract, ethyl acetate fraction and aglycone fraction on ROS generated in $Fe^{3+}-EDTA/H_2O_2$ system using the luminol-dependent chemiluminescence assay were 37.15, 10.74, $7.19{\mu}g/mL$, respectively. We investigated the cellular protective activity and the results showed that treatment of aglycone fraction ($0.05-0.39{\mu}g/mL$) protect human skin cells in a concentration-dependent manner when the skin cell damages were induced by treating them with $H_2O_2$. These results suggest that extract/fractions of G. pentaphyllum Makino may be applicable as natural antioxidants in cosmetics.

Quantitative Analysis of Pulegone in Schizonepeta tenuifolia Briq. (국산과 중국산 형개의 Pulegone함량분석)

  • Kim, Ho-Kyoung;Lee, A-Yeong;Lee, Hye-Won;Chun, Jin-Mi;Choi, Ji-Hyun;Shin, Ja-Won;Ko, Byoung-Seob
    • Applied Biological Chemistry
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    • v.49 no.2
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    • pp.125-128
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    • 2006
  • Schizonepeta tenuifolia Briq. are used in folk medicine as common cold, sore throat, headache and skin infection in Korea, China and Japan. To compare the contents of domestic and Chinese Schizonepeta tenuifolia quantitative HPLC analysis was performed using a Luna $C_{18}$ column $(4.6{\times}250\;mm,\;5{\mu}m)$, with 1 ml/min of flow rate of 0.01% formic acid in water : acetonitrile = 50 : 50 under UV 254 nm of detector. Pulegone was detected at retention time of about 17.14 min.

Development of PC Based Signal Postprocessing System in MR Spectroscopy: Normal Brain Spectrum in 1.5T MR Spectroscopy (PC를 이용한 자기공명분광 신호처리분석 시스템 개발: 1.5T MR Spectroscopy에서의 정상인 뇌 분광 신호)

  • 백문영;강원석;이현용;신운재;은충기
    • Investigative Magnetic Resonance Imaging
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    • v.4 no.2
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    • pp.128-135
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    • 2000
  • Purpose : The aim of this study is to develope the Magnetic Resonance Spectroscopy(MRS) data processing S/W which plays an important role as a diagnostic tool in clinical field. Materials and methods : Post-processing software of MRS based on graphical user interface(GUI) under windows operating system of personal computer(PC) was developed using MATLAB(Mathwork, U.S.A.). This tool contains many functions to increase the quality of spectrum data such as DC correction, zero filling, line broadening, Gauss-Lorentzian filtering, phase correction, etc. And we obtained the normal human brain $^1H$ MRS data from parietal white matter, basal ganglia and occipital grey matter region using 1.5T Gyroscan ACS-NT R6 (philips, Amsterdam, Netherland) MRS package. The analysis of the MRS peaks were performed by obtaining the ratio of peak area. Results : The peak ratios of NAA/Cr, Cho/Cr, MI/Cr for the different MRS machines have a little different values. But these peak ratios were not significantly different between different echo time MRS peak ratios in the same machine (p<0.05). Conclusion : MRS post-processing S/W based on GUI using PC was developed and applied to the analysis of normal human brain $^1H$ MRS. This independent MRS processing job increases the performance and throughput of patient scan of main console. Finally, we suggest that the database for normal in-yivo human MRS data should be obtained before clinical applications.

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Development of a Greenhouse Environment Monitoring System using Low-cost Microcontroller and Open-source Software (저비용 개방형 Microcontroller를 사용한 온실 환경 측정 시스템 개발)

  • Cha, Mi-Kyung;Jeon, Youn A;Son, Jung Eek;Chung, Sun-Ok;Cho, Young-Yeol
    • Horticultural Science & Technology
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    • v.34 no.6
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    • pp.860-870
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    • 2016
  • Continuous monitoring of environmental parameters provides farmers with useful information, which can improve the quality and productivity of crops grown in greenhouses. The objective of this study was to develop a greenhouse environment measurement system using a low-cost microcontroller with open-source software. Greenhouse environment parameters measured were air temperature, relative humidity, and carbon dioxide ($CO_2$) concentration. The ranges of the temperature, relative humidity, and $CO_2$ concentration were -40 to $120^{\circ}C$, 0 to 100%, and 0 to 10,000 ppm, respectively. A $128{\times}64$ graphic LCD display was used for real-time monitoring of the greenhouse environments. An Arduino Uno R3 consisted of a USB interface for communicating with a computer, 6 analog inputs, and 14 digital input/output pins. A temperature/relative humidity sensor was connected to digital pins 2 and 3. A $CO_2$ sensor was connected to digital pins 12 and 13. The LCD was connected to digital pin 1 (TX). The sketches were programmed with the Arduino Software (IDE). A measurement system including the Arduino board, sensors, and accessories was developed (totaling $244). Data for the environmental parameters in a venlo-type greenhouse were obtained using this system without any problems. We expect that the low-cost microcontroller using open-source software can be used for monitoring the environments of plastic greenhouses in Korea.