• Title/Summary/Keyword: methylene-blue

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Medium Effect on the Formation of Ion-Pair between Methylene Blue and Tetraphenylborate in Dilute Solutions

  • Moon, Eui-Wha;Lee, Beom-Gyu;Kim, Kang-Jin
    • Bulletin of the Korean Chemical Society
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    • v.9 no.4
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    • pp.209-212
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    • 1988
  • The hydrophobic interaction leading to the formation of ion-pair between $MB^+$ and $TPB^-$ was investigated spectroscopically by varying the medium with the addition of 1,A-dioxane or urea. Beyond 0.01 mole fraction of 1,A-dioxane in water or above 2.0M urea the ion-pair appeared to be completely dissociated into individual ions. The ion-pair was not observed in common organic solvents and the absorption maxima of $MB^+$ were correlated relatively well with the ${\pi}^{\ast}$-scale.

A Study on the Analysis Method of Soil Dust Included in the Aggregate Fines (골재 미립분에 포함된 토분의 분석방법 고찰)

  • Min, Tae-Beom;Kim, In;Lee, Jae-Sam;Baek, Chul-Seoung
    • Proceedings of the Korean Institute of Building Construction Conference
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    • 2023.05a
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    • pp.257-258
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    • 2023
  • As a result of examining research contents related to soil excretion being studied in Korea, there are five methods for soil excretion testing. As a result of analyzing the correlation between each test method and concrete compressive strength, the chemical test method and the Methylene blue test showed a high correlation index.

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Oxidation of Carbon Monoxide by Pseudomonas carboxydohydrogena (Pseudomonas carboxydohydrogena에 의한 일산화탄소의 산화)

  • ;Hegeman, George
    • Korean Journal of Microbiology
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    • v.21 no.1
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    • pp.27-35
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    • 1983
  • The stoichiometry between the consumption of CO and $O_2$ and the production of $CO_2(2CO+O_2{\rightarrow}2CO_2)$) showed that Pseudomonas carboxydohydrogena grows as a typical aerobic CO oxidizer with CO. The optimal concentration of CO for growth was found to be 30% in gas mixture with air. The initial buffer concentration of the culture medium did not affect the growth of this bacterium. P. carboxydohydrogena is an obligate aerobe and dose not use nitrate as a terminal electron acceptor. The CO dehydrogenase is an inducible and soluble enzyme. The reaction rate and stability were maximal at pH7.5, and the Arrhenius plot revealed an activation energy of 37.7kJ/mol (9.0 Kcal/mol). The crude enzyme used methylene blue, thionin, and toluylene blue as electron acceptors for the oxidation of CO to $Co_2$ under anaerobic conditions. It was found that water must be the source of the second oxygen atom for CO oxidation.

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Decolorization of Dyes by Selected Wood Degradation Fungus

  • Lee, Sung-Wook;Park, Heon;Min, Kyeong-Heui
    • Journal of the Korean Wood Science and Technology
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    • v.32 no.3
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    • pp.79-87
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    • 2004
  • The objectives of this study were to select superior fungus for lignin degradation and to decolor dyes by selected fungus. Ligninolytic fungi were screened and isolated from decayed woods. Ten ligninolytic fungi were selected by ligninolytic enzyme activity on the PDA media containing rhemazol brilliant blue R, guaiacol and gallic acid. Their lignin degradation abilities were tested on the extractive-free wood powder of Quercus acutissima and Pinus densiflora. As a result, 8J-28 was selected as superior fungus for lignin degradation. Also, decolorization abilities of dyes were examined by shaking and static culture. And congo red, crystal violet, poly R-478, methylene blue used to investigate decolorization abilities of dyes. As a result, 8J-28 showed over 90% in decolorization of congo red, crystal violet, poly R-478.

Synthesis of Nanorod g-C3N3/Ag3PO4 Composites and Photocatalytic Activity for Removing Organic Dyes under Visible Light Condition

  • Se Hwan Park;Jeong Won Ko;Weon Bae Ko
    • Elastomers and Composites
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    • v.59 no.1
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    • pp.1-7
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    • 2024
  • Nanorod graphitic carbon nitride (g-C3N4) was synthesized by reacting melamine (C3H6N6) with trithiocyanuric acid (C3H3N3S3) in distilled water for 10 h at room temperature. The resulting mixture was calcined at 550℃ for 2 h in an electric furnace under an air atmosphere. Nanorod g-C3N4/Ag3PO4 composites were prepared by adding nanorod graphitic carbon nitride (g-C3N4) powder, silver nitrate (AgNO3), ammonia (NH3·H2O, 25.0-30.0%), and sodium hydrogen phosphate (Na3HPO4) to distilled water. The samples were characterized via X-ray diffraction, scanning electron microscopy, and Fourier-transform infrared spectroscopy. The photocatalytic activities of the nanorod g-C3N4/Ag3PO4 composites were demonstrated via the degradation of organic dyes, such as methylene blue and methyl orange, under blue light-emitting diode irradiation and evaluated using UV-vis spectrophotometry.

Effects of Phamacopuncture Therapy Using Cervi Pantotrichum Cornu at BL23.BL52 on the Cerebral Hemodynamics in Rats (신유.지실 녹용약침이 뇌혈류역학에 미치는 영향)

  • Lee, Seok-Jin;Jeong, Hyun-Woo
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.23 no.1
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    • pp.50-56
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    • 2009
  • The present study was designed to investigate the effects of Phamacopuncture therapy using Cervi Pantotrichum Cornu (PC) at BL23 BL52 on the regional cerebral blood flow (rCBF) and mean arterial blood pressure (MABP) in normal rats, then the related mechanisms were also investigated. In addition, the present author also investigated the effects of phamacopuncture therapy at BL23.BL52 on the rCBF in cerebral ischemic rats. The results in normal rats were as follows; PC (3 mg/kg and 5 mg/kg) at BL23 BL52 significantly increased rCBF but decreased MABP. This result suggests that PC at BL23 BL52 significantly increased rCBF by dilating pial arterial diameter. Increase of PC (5 mg/kg)-induced rCBF was significantly inhibited by pretreatment with indomethacin (1 mg/kg, i.p.), an inhibitor of cyclooxygenase and methylene blue ($10{\mu}g/kg$, i.p.), an inhibitor of guanylate cyclase. Decrease of PC (5 mg/kg)-induced MABP was significantly increased by pretreatment with methylene blue but was decreased by pretreatment with indomethacin. These results suggested that the action of PC (5 mg/kg) was mediated by guanylate cyclase. The results in cerebral ischemic rats were as follows ; The rCBF was significantly and stably increased by PC (5 mg/kg) during the period of cerebral reperfusion, which contrasted with the findings of rapid and marked increase in Control group. In conclusion, these results suggest that phamacopuncture therapy using Carthami flos at BL23 BL52 can increase rCBF in normal state, and improve stability of rCBF in ischemic state. In addition, the present author also suggest that related mechanisms are involved in guanylate cyclase pathway.

Degradation of Three Aromatic Dyes by White Rot Fungi and the Production of Ligninolytic Enzymes

  • Jayasinghe, Chandana;Imtiaj, Ahmed;Lee, Geon-Woo;Im, Kyung-Hoan;Hur, Hyun;Lee, Min-Woong;Yang, Hee-Sun;Lee, Tae-Soo
    • Mycobiology
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    • v.36 no.2
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    • pp.114-120
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    • 2008
  • This study was conducted to evaluate the degradation of aromatic dyes and the production of ligninolytic enzymes by 10 white rot fungi. The results of this study revealed that Pycnoporus cinnabarinus, Pleurotus pulmonarius, Ganoderma lucidum, Trametes suaveolens, Stereum ostrea and Fomes fomentarius have the ability to efficiently degrade congo red on solid media. However, malachite green inhibited the mycelial growth of these organisms. Therefore, they did not effectively decolorize malachite green on solid media. However, P. cinnabarinus and P. pulmonarius were able to effectively decolorize malachite green on solid media. T. suaveolens and F. rosea decolorized methylene blue more effectively than any of the other fungi evaluated in this study. In liquid culture, G. lucidum, P. cinnabarinus, Naematoloma fasciculare and Pycnoporus coccineus were found to have a greater ability to decolorize congo red. In addition, P. cinnabarinus, G lucidum and T. suaveolens decolorized methylene blue in liquid media more effectively than any of the other organisms evaluated in this study. Only F. fomentarius was able to decolorize malachite green in liquid media, and its ability to do so was limited. To investigate the production of ligninolytic enzymes in media containing aromatic compounds, fungi were cultured in naphthalene supple mented liquid media. P. coccineus, Coriolus versicolor and P. cinnabarinus were found to produce a large amount of laccase when grown in medium that contained napthalene.

Effects of BanhabackchulChunma-tang on the Vascular Relaxation and its mechanism (혈관 긴장성 조절에 미치는 반하백출천마탕)

  • Kim Yon Tae;Chae Jong Koo;Kim Gil Whon;Shin Heung Mook
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.16 no.1
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    • pp.62-66
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    • 2002
  • Hypertension is not only a well-established cardiovascular risk factor but also increase the risk of atherosclerosis. Most studies conducted to investigate the effectiveness of treatment for cardiovascular disease such as hypertension have focused primarily on conventional drug and physiotherapeutic treatments. BanhabackchulChunma-tang(半夏白朮天麻湯:BCT) is popular herbal medicine used in clinic for the treatment of various symptoms of drulatory disorders and weakness of digestive system, including anorexa and nausea with vertigo, severe headache, vomiting and so on. However, the mechanisms underlying its efficacy are unknown. This study investigated the effects of BCT as an alternative medication on the contraction induced by phenylephrine and KCI in rat thoratic aorta. BCT revealed siginificant relaxation on phenylephrine-induced arterial contraction, but revealed noncompetitive effect on concentration responses of phenylephrine-induced contraction. Treatment of N-L/sup ω/ -argine methyl ester(L-NAME) and methylene blue(MB)(10/sup -5/M) reduced the relaxation of BCT. BCT also increased in vitro NO production. It suggest that the relaxation effect of BBT is related with NO pathway, becausse the effect of L-NAME and MB are due to inhibition of NO synthesis from endothelial cells. These results indicate that BCT would be effective in hypertension treatment and its mechanism of relaxtion on arterial contraction is likely to be related with NO production, blocking of α-receptor and signal transduction after receptor activation.

Effects of the Endothelium on the Contractile Responses to Norepinephrine in Isolated Proximal and Distal Coronary Artery of Pigs

  • Kim, Jong-Hoon;Jeon, Byeong-Hwa;Chang, Seok-Jong;Park, Hae-Kun
    • The Korean Journal of Physiology
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    • v.27 no.1
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    • pp.27-35
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    • 1993
  • Effects of the endothelium on the contractile responses to norepinephrine (NE) were investigated in isolated helical strips of the proximal and distal coronaries artery of pigs. The helical strips were immersed in Tris-buffered Tyrode's solution equilibrated with 100% $O_2$ at $35^{\circ}C$ and its isometric tension was measured. NE relaxed the strips precontracted with acetylcholine from both the proximal and distal coronary artery. NE-induced relaxation, which might be induced mainly by $\beta$-adrenoceptor function was dominant in the distal coronary arteries. NE-induced relaxation was converted to a contraction after $\beta$-adrenoceptor blockade with propranolol $(3{\times}10^{-6}M)$. ${\alpha}$-adrenoceptor-mediated contraction by NE was greater in the proximal coronary artery than the distal coronary artery. Quantitatively, ${\alpha}_1$-adrenoceptor mediated contraction by NE was greater than ${\alpha}_2$-adrenoceptor mediated contraction by NE in both arteries. NE-induced relaxation was decreased by rubbing of endothelium in both arteries. ${\alpha}_1-and\;{\alpha}_2$-adrenoceptor mediated contraction by NE were potentiated by rubbing of endothelium in both arteries. Pretreatment with methylene blue, an inhibitor of soluble guanylate cyclase, increased ${\alpha}_1-\;and\;{\alpha}_2$-adrenoceptor mediated contraction by NE in both arteries with endothelium. From the above results, we suggest that the effect of activation of $\alpha$-adrenoceptors by NE may be modulated by endothelium in the proximal and distal coronary arteries of pigs. This effect may be mediated via endothelium derived relaxing factor.

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Efficiency Characteristics of Dye-Sensitized Solar Cells with Heat Treatment Temperature of P-25 Photocatalyst (P-25 광촉매의 열처리 온도에 따른 염료감응태양전지의 효율특성)

  • Park, Ki-Min;Kim, Tae-Young;Kim, Jeong-Guk;Cho, Sung-Yong
    • Korean Chemical Engineering Research
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    • v.48 no.5
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    • pp.649-653
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    • 2010
  • In this study, the variation of characteristics of P-25 photocatalyst with calcination temperature(Pure: non-thermally treated, 450, 650, $850^{\circ}C$) was studied. The photocatalysts were used as working materials for dye-sensitized solar cells: DSSCs) later on and their photovoltaic characterization was carried out. The photocatalytic degradation of methylene blue using the P-25 photocatalyst with different calcination temperature was almost same expect for $850^{\circ}C$. The solar energy conversion efficiency ($\eta$) of DSSCs prepared by the nanoparticles (photocatalyst) reached 6.9% (for pure), 6.5%(for 450), 5.8%(for 650) and 5.6%(850).