• Title/Summary/Keyword: methyl sinapate

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A feruloyl esterase derived from a leachate metagenome library

  • Rashamuse, Konanani;Sanyika, Walter;Ronneburg, Tina;Brady, Dean
    • BMB Reports
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    • v.45 no.1
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    • pp.14-19
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    • 2012
  • A feruloyl esterase encoding gene (designated fae6), derived from a leachate metagenomic library, was cloned and the nucleotide sequence of the insert DNA determined. Translational analysis revealed that fae6 consists of a 515 amino acid poly-peptide, encoding a 55 kDa pre-protein. The Fae6 primary structure contained the G-E-S-A-G sequence, which corresponds well with a typical catalytic serine sequence motif (G-x-S-x-G). The fae6 gene was successfully over-expressed in E. coli and the recombinant protein was purified to 8.4 fold enrichment with 17% recovery. The $K_M$ data showed Fae6 has a high affinity to methyl sinapate while thermostability data indicated that fae6 was thermolabile with a half life ($T_{1/2}$) < 30 min at $50^{\circ}C$. High affinity for Fae6 against methyl sinapate, methyl ferulate and ethyl ferulate suggest that the enzyme can be useful in hydrolyzing ferulated polysaccharides in a biorefinery process.

Studies on the Components of Evodia daniellii $H_{EMSLEY}$ in Korea (한국산 오수유성분에 관한 연구)

  • Chung, Bo-Sup
    • Korean Journal of Pharmacognosy
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    • v.1 no.4
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    • pp.119-124
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    • 1970
  • Evodia daniellii $H_{EMSLEY}$, a plant belonging to Rutaceae, is cultivated in Korea and used as a folkmedicine for gastric inflammation, extermination of noxious insects, and headache. The seed oil of this plant also has been used in various diseases, for example, dermatitis, scabies and so forth. From the barks, fruit peels, and seed oil of Evodia daniellii $H_{EMSLEY}$, four crystalline compounds were isolated. Three compounds except one were characterized as methyl sinapate, bergapten, and evodiamine by m.p. determination, elemental analysis, UV, IR, NMR spectra and mass analysis. Even though methyl sinapate was a known synthetic compound, it was not yet reported as a natural product. From the seed oil, unsaponifiable fraction was separated and was analyzed. It was considered to be consisted with sterols, hydrocarbons and tocopherols.

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Isolation of Sinapic Acid Esters from Raphani Semen (내복자(Raphani Semen)로부터 Sinapic acid esters의 분리)

  • Kang, Eun-Jung;Ko, Byoung-Seob;Kim, Ho-Kyoung
    • Korean Journal of Pharmacognosy
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    • v.31 no.4
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    • pp.434-437
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    • 2000
  • Two sinapic acid esters were isolated from the methanol extract of Raphani Semen. The structures were elucidated on the basis of spectroscopic methods $(^1H-NMR,\;^{13}C-NMR,\;HMQC,\;^1H-^1H\;COSY\;and\;HMBC)$ and were identified as methyl sinapate (1) and ${\beta}-D-(3-O- sinapoyl)fructofuranosyl-{\alpha}-D-(6-O-sinapoyl)glucopyranoside$ (2). Compound 1 was first isolated from the genus of Raphanus.

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