• 한국근적외분광분석학회:학술대회논문집
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• 한국근적외분광분석학회 2001년도 NIR-2001
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• pp.1258-1258
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• 2001

Estimation of sugar and reducing sugar content in molasses is very important task in sugar refineries. Conventional methods of determination of sugar content in molasses samples are highly time consuming and employ hazardous chemicals. Due to the physical properties of molasses, probability of error in conventional analytical techniques is high. These methods have proven to be inefficient for a process control in any sugar industry. Hence development of a rapid, inexpensive, physical and also accurate method for sugar determination in molasses will be highly useful. Near Infrared spectroscopy is being widely used worldwide as an analytical technique in food industry. The technique offers the advantage of being non-destructive and rapid. The present paper highlights the potential of near infrared reflectance spectroscopy as a rapid and automated analytical technique for determination of sugar and reducing sugar content in molasses. A number of molasses samples were collected during and after the sugar season from Havana Sugar Industry, Havana. The samples were chosen so as to obtain a wide range of concentration of sugar and reducing sugars. This was done in order to achieve a good calibration curve with widely spread data points. These samples were scanned in the region of 1100 - 2500 nm in diffuse reflectance mode. An indigenous ELICO NIR spectrophotometer, modified according to the requirements of sugar industry was used for this purpose. Each sample was also analyzed simultaneously by standard chemical methods. Chemical values were taken as reference for near infrared analysis. In order to obtain the most accurate calibration for the set of samples, various mathematical treatments were employed. Partial Least Square method was found to be most suitable for the analysis. A comparison is made between the actual values (chemical values) and the predicted values (NIR values). The actual values agree very well with the predicted values showing the accuracy of the technique. The validity of the technique is checked by predicting the concentration of sugar in unknown molasses samples using the calibration curve. The present investigation assesses the feasibility of the technique for on-line monitoring of sugars present in molasses in sugar industries.

• 한국환경농학회지
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• 제17권4호
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• pp.371-378
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• 1998

Pesticide has played important role in Korean and Austrailian agriculture. In addition, pesticides are the most reliable tools pests in agriculture. Recently, it is highly recommended that the use of pesticide should be concerned with both atricultural and environmental aspect, also legislation on environmental contamination has been fortified to the world. Particularly, the attention on agrochemicals has been focused on the soil abuse and the water contamination at present time. In spite of this kind of concern, a few research about pesticides using in Australia and Korean have been conducted to their behaviors under australian and korean environment to avoid environmental contamination by pesticides. Thus, the research organizations need facilities to analyze the characteristics of each pesticide and the environmental fate of pesticides. The conventional analytical method to detect pesticides and their metabolites can not be overcome to reduce time, expenditure, and complexity of analysis even though the methods are accurate and precise. For example, High performance liquid chromatography(HPLC), and gas chromatography (GC) used until now are less choice detectors and often lower sensitive. In contrast to the conventional analytical methods, biosensors are so fast in analysis and has high productivity and analyze multi=sample simultaneously. Therefore, it is biosensing analytical method that we could consider as an alternative method intead of the conventional methods.

• 한국지진공학회논문집
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• 제7권6호
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• pp.109-117
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• 2003

기존의 비선형 정적 및 동적 해석에서는 철근콘크리트 구조물의 에너지 소산능력을 정확히 고려하지 못하고 있다. 최근 연구에서는 휨지배 철근콘크리트 부재의 에너지 소산능력을 정확히 평가할 수 있는 식이 개발되었으며, 본 연구에서는 이 평가방법을 이용하여 에너지 소산능력을 정확히 고려할 수 있는 비선형 정적 및 동적 해석 방법을 개발하였다. 비선형 정적 해석을 위하여 에너지 스펙트럼 곡선을 개발하고 이를 적용하여 능력스펙트럼법을 개선하였으며, 또한 비선형 동적 해석을 위하여 철근콘크리트 부재의 단순화된 에너지 기초 주기거동모델을 개발하였다. 제안된 모델은 부재의 강성에 기초한 기존의 주기거동모델과는 달리 완전한 주기거동 발생시 소산되는 에너지를 정확하게 반영할 수 있다. 본 연구에서는 제안된 방법에 따라 비선형 정적 및 동적 해석법의 절차를 정립하였으며 이를 적용한 컴퓨터 해석 프로그램을 개발하였다. 제안된 해석 방법은 부재의 단면형태, 철근비, 배근형태 등 설계 변수에 따른 에너지 소산능력을 정확하게 고려하고 지진발생시 에너지 소산능력이 구조물의 성능에 미치는 효과를 반영할 수 있다.

• 한국환경보건학회지
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• 제28권2호
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• pp.109-116
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• 2002

The purpose of this study was to evaluate a new sampling and analytical method for the determination of airborne hexavalent chromium, Cr(Ⅵ), in a field plating operation. The procedures of this new method (Shin & Paik's Method) are as the following: Airborne hexavalent chromium is collected on polyvinyl chloride (PVC) filter according to the National Institute iota Occupational Safety and Health (NIOSH) Method 7600, and the filler sample is placed in a screw-capped vial and soaked with 2% NaOH/3% Na₂CO₃ solution immediately after sampling. The Cr(Ⅵ) sample is analyzed by ion chromatography/visible spectrophotometry (IC/VS) according to the U.S. Environmental Protection Agency (EPA) Method 218.6. The airborne Cr(Ⅵ) concentrations measured by this method were compared with those determined by three reference methods: One (NIOSH/EPA Method) consisted of sampling airborne Cr(Ⅵ) on PVC filters and storing the sample filters in strew-capped vials according to the NIOSH method, and analyzing Cr(Ⅵ) in samples using IC/VS according to the EPA method. The second method (Impinger Method/NaHCO₃) consisted of absorbing airborne Cr(Ⅵ) into 0.02 M NaHCO₃ solution in midget impinger, and analyzing the Cr(Ⅵ) in samples using IC/VS. The third method was the OSHA Method ID-215. Using these four different methods, lour replicates of air samples were collected at an electroplating process and analyzed simultaneously. Two-way ANOVA and paired t-test were used to test difference among values determined by the methods. There was no significant difference and a strong correlation (r²:0.99) between Cr(Ⅵ) concentrations measured by the Shin & Paik's Method and an impinger method (p>0.05). However, Cr(Ⅵ) concentrations determined by Shin & Paik's Method were significant1y different from those by the NIOSH/EPA Method (p<0.05) or the OSHA method (p<0.05). The Cr(Ⅵ) concentrations of Shin & Paik's Method were significantly higher than those of the NIOSH/EPA Method or the OSHA method. This result indicated that the Shin & Paik's Method may prevent Cr(Ⅵ) losses caused by reduction and give more reliable results of airborne Cr(Ⅵ) concentrations in work environments.

• 한국산업보건학회지
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• 제26권1호
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• pp.20-29
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• 2016

Objectives: The purpose of this study is to simplify and standardize analytical method of polar solvents(methanol, isopropanol, n-butanol, acetone, methylene chloride and MIBK) in the working environmental by GC. Because NIOSH methods are various and complicated. Methods: The method is using the same stationary phase(5% phenyl 95% dimethylpolysiloxane), absorbent(silica gel) and desorption solvent(DMSO) for above 6 solvents. For the 6 solvents desorption efficiency, calibration curve, and limit of detection were studied Results: As the results, 6 solvents{2 groups ; first group(methanol/isopropanol/butanol) and second group(acetone/methylene chloride/MIBK)} could be separated and quantified within 10 minutes. Desorption efficiency from silica gell of 6 solvents using dimethylsulfoxide(DMSO) was methanol 86.2%, isopropanol 103.2%, n-butanol 101.8%, acetone 98.2%, methylene chloride 103.9% and MIBK 106.2% in the range of 0.2, 0.5, 2.0 times of TWA, consequently, satisfied NIOSH estimation level(beyond 75%). Correlation coefficient(r)in the range of 0.2~2.0 times of TWA, was above 0.999 for 6 solvent. LOD(mg/DMSO ml) using calibration curve in the range of 0.2~2 times of TWA was methanol 0.11, isopropyl alcohol 0.20, n-butanol 0.03, acetone 0.50, methylene chloride 0.05, MIBK 0.04 respectably. Conclusions: This method can be used at the sampling and analytical method for polar solvents by GC. Also, will be able to be applied with NIOSH methods.

• 한국식품위생안전성학회지
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• 제27권1호
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• pp.1-17
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• 2012

T-2 toxin and HT-2 toxin, belong to type A trichothecences, are the most toxic mycotoxins among the trichothecene family. These mycotoxins are commonly found in cereals such as maize, wheat, barley, oats and rice, and their occurrence in food can be of concern. This review investigated the current trends of patents and researches on T-2 toxin and HT-2 toxin pertaining to natural occurrence, toxicity, metabolism, risk assessment, analytical and screening methods, and reduction/detoxification techniques. As compared with other $Fusarium$ mycotoxins, there are limited data for natural occurrence and risk assessment, and regulatory limit and official analytical methods on T-2 toxin and HT-2 toxin in domestic and foreign countries. In particular, selective deacetylation at the C3 and/or C4 positions of T-2 toxin by carboxyesterase present in foods was reported to cause the disappearance of T-2 and the extremely high HT-2 recoveries. Currently, regulatory limits for T-2 and HT-2 are under discussion in EU. For enforcement purposes it is essential to have available precise and reliable analytical methods applicable at the regulatory levels for the T-2 toxin and HT-2 toxin and relevant commodities. In addition, a further study on natural occurrence, risk assessment and reduction/detoxification techniques will be recommended.

• 분석과학
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• 제33권2호
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• pp.98-107
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• 2020

Methamphetamine (MA) is currently the most abused illicit drug in Korea. MA is produced by chemical synthesis, and the final target drug that is produced contains small amounts of the precursor chemicals, intermediates, and by-products. To identify and quantify these trace compounds in MA seizures, a practical and feasible approach for conducting chromatographic fingerprinting with a suite of traditional chemometric methods and recently introduced machine learning approaches was examined. This was achieved using gas chromatography (GC) coupled with a flame ionization detector (FID) and mass spectrometry (MS). Following appropriate examination of all the peaks in 71 samples, 166 impurities were selected as the characteristic components. Unsupervised (principal component analysis (PCA), hierarchical cluster analysis (HCA), and K-means clustering) and supervised (partial least squares-discriminant analysis (PLS-DA), orthogonal partial least squares-discriminant analysis (OPLS-DA), support vector machines (SVM), and deep neural network (DNN) with Keras) chemometric techniques were employed for classifying the 71 MA seizures. The results of the PCA, HCA, K-means clustering, PLS-DA, OPLS-DA, SVM, and DNN methods for quality evaluation were in good agreement. However, the tested MA seizures possessed distinct features, such as chirality, cutting agents, and boiling points. The study indicated that the established qualitative and semi-quantitative methods will be practical and useful analytical tools for characterizing trace compounds in illicit MA seizures. Moreover, they will provide a statistical basis for identifying the synthesis route, sources of supply, trafficking routes, and connections between seizures, which will support drug law enforcement agencies in their effort to eliminate organized MA crime.

• Journal of the Korean Wood Science and Technology
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• 제36권3호
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• pp.24-29
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• 2008

수치해석 모델을 적용하여 다중 볼트 접합부의 거동을 해석하였다. 기본 모델로 축력-휨 요소를 가정하고 해석을 실시하였으며, 프레임재의 효과와 철물의 효과를 구분하여 해석을 실시하였다. 기존의 해석적인 방법은 볼트의 개수가 2개까지만으로 제한될 경우는 어느 정도 정확도를 보여 줄 수 있어서 많은 시간이 소요되는 수치해석적 방법보다 장점이 있을 수 있다. 하지만 현실적으로 사용되고 있는 현장의 조건은 그 이상의 볼트를 사용하고 있는 실정이다. 또한 현장에서 사용되고 있는 볼트의 배치 및 배열 형태를 기존의 해석적 방법을 적용하여 거동 분석을 실시한다는 것은 불가능하다. 이러한 현실 조건을 고려하여 볼트 접합부의 정확한 거동해석을 실시하기 위해서는 수치해석적 방법을 적용하는 방법 이외에는 대안이 없다. 다양한 다중 볼트 접합부에 대해 수치해석 모델을 적용한 결과 광범위하게 사용되고 있는 항복모델 등의 기존의 경험적 해석방법에 비해 현실적인 접합부 여건 반영이 수월하고 정확성에 있어서 우수함을 볼 수 있었다.

• Journal of Ginseng Research
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• 제45권5호
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• pp.539-545
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• 2021

Background: Red ginseng polysaccharides (RGPs) have been acknowledged for their outstanding immunomodulation and anti-tumor activities. However, their studies are still limited by the complexity of their structural features, the absence of purification and enrichment methods, and the rarity of the analytical instruments that apply to the analysis of such macromolecules. Thus, this study is an attempt to establish a new mass spectrometry (MS)-based analysis procedure for RGPs. Methods: Saponin pre-excluded powder of RG (RG-SPEP, 10 mg) was treated with 200 µL of distilled water and centrifuged for 5 h at 1000 rpm and 85 ℃. Ethanol-based precipitation and centrifugation were applied to obtain RGPs from the heated extracts. Further, endo-carbohydrase treatments were performed to produce specific saccharide fragments. Solid-phase extraction (SPE) processes were implemented to purify and enrich the enzyme-treated RGPs, while matrix-assisted laser desorption/ionization time-of-flight/time-of-flight (MALDI-TOF/TOF) MS was employed for the partial structural analysis of the obtained RGPs. Results: Utilizing cellulase, porous graphitized carbon (PGC), hydrophilic interaction chromatography (HILIC), and MALDI-TOF/TOF MS, the neutral and acidic RGPs were qualitatively analyzed. Hex_n and Hex_n-18 (cellulose analogs) were determined to be novel neutral RGPs. Additionally, the [Unknown + Hex_n] species were also determined as new acidic RGPs. Furthermore, HexA_n (H) was determined as another form of the acidic RGPs. Conclusion: Compared to the previous methods of analysis, these unprecedented applications of HILIC-SPE and MALDI-TOF/TOF MS to analyze RGPs proved to be fairly effective for fractionating and detecting neutral and acidic components. This new procedure exhibits great potential as a specific tool for searching and determining various polysaccharides in many herbal medicines.

• 한국축산식품학회지
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• 제39권1호
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• pp.93-101
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• 2019

Nucleotides play important roles in numerous intracellular biochemical processes and are used in infant formulas and other dairy products. However, domestic analytical methods for assessing nucleotide content in products have not yet been established, and therefore, methods for determining nucleotide content are urgently required. A rapid and simple analytical method for determining the content of five types of nucleotides in dairy products was improved using solid phase extraction clean-up and high-performance liquid chromatography with diode array detector. The extraction solvent used in the AOAC method was not well dissolved and was changed to hydrophilic EDTA-Na. In addition, the results obtained using the isocratic elution method and a single wavelength were similar to those obtained using the AOAC method, and the time taken for analysis was shortened from 40 min to 25 min. The process of method validation revealed the following parameters: accuracy (84.69%-102.72%), precision (1.51%-6.82%), linearity (0.999), and limit of detection (cytidine 5'-monophosphate, 0.09 mg/L; uridine 5'-monophosphate, 0.11 mg/L; adenosine 5'-monophosphate, 0.12 mg/L; guanosine 5'-monophosphate, 0.11 mg/L; and inosine 5'-monophosphate, 0.14 mg/L). The method was also used to determine the nucleotide concentration in 25 samples (infant formulas, 1.99-29.39 mg/100 g; and cow milk, 0.28-0.83 mg/100 g). The newly improved method was appropriate for analyzing nucleotides in infant formulas and other dairy products faster when compared to conventional methods.