• Journal of Applied Biological Chemistry
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• 제50권4호
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• pp.288-292
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• 2007

• 대한가정학회지
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• 제19권1호
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• pp.47-52
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• 1981

In the view of the fact that data are scare concerning the change of Methionine composition of poultry meat in function of storage temperature and cooking method. The present study was carried out to change in these compounds and the results were summarized as follows. 1. The result of measuring Methionine compound of Raw material stored under the condition of $5^{\circ}C$, $10^{\circ}C$ for 6 weeks were increased as temperature increases. 2. The result of change in Methionine compound of Roasting were deceased than Raw material It was greatly decreased in the storage for 42 days at 1$0^{\circ}C$ and slightly decreased in the storage for 14 days at $5^{\circ}C$. 3. when the Boiling in the water, Methoinine compound was decreased than Roasting to compare with Raw material 4. By the results of this study, one of the best cooking method is Roasting, Because of the change I Methionine compound of Roasting is slightly decrease.

• Journal of Applied Biological Chemistry
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• 제49권1호
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• pp.8-10
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• 2006

Compositions of methionine-metal chelates have been investigated by FT-IR and XRD studies to elucidate their molecular structures. It was concluded that Copamin and Zincamin contain a high percentage of crystalline products, presumably 2:1 Methionine-Cu or Zn complexes. On the contrary, FT-IR and XRD spectra of Ferramin didn't show any characteristics of the chelate and it was concluded to contain major components of starting $FeSO_4$ and methionine without chelation.

• Journal of Pharmaceutical Investigation
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• 제24권1호
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• pp.1-9
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• 1994

In order to study systemic peptide delivery through the ocular route, the stabilities of methionine enkephalin (Met-Enk) and $[D-ala^2]-methionine$ enkephalinamide (YAGFM) in the corneal extracts of rabbits were investigated using reversed phase HPLC. Met-Enk was found to be hydrolyzed most rapidly in the corneal epithelium, but YAGFM was relatively stable. Aminopeptidases appeared to contribute over 60% to the degradation of Met-Enk and the degradation rate of Met-Enk followed the first order kinetics. The half-lives of Met-Enk in the extracts of the corneal epithelium and endothelium were 36 and 673 min, respectively. From the effects of enzyme inhibitors, it was found that the application of the mixture of amastatin, thimerosal and EDTA was very useful for the inhibition of peptide degradation.

• Asian-Australasian Journal of Animal Sciences
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• 제16권5호
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• pp.714-718
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• 2003

This study was conducted to determine the effect of DL-methionine (Met) and DL-methionine hydroxy analogue (MHA) supplementation on the growth performance, contents of serum amino acids and activities of digestive protease in broiler chicks from 0 to 3 weeks old . There were three treatments in this study: (1) control (basal diet), (2) 0.24% Met supplementation and (3) 0.368% MHA supplementation. The results showed that Met and MHA supplementation did not significantly (p>0.05) improve feed efficiency and body weight gain for broilers from 0-1, 0-2 and 0-3 weeks of age. The serum levels of homocystine, methionine and taurine were significantly (p<0.05) higher with supplementation of Met or MHA than with control. The pepsin activity of proventriculus was increased with (p<0.05) Met supplementation at 21 days of age and with MHA supplementation at 7 and 14 days of age. The trypsin activity was also increased (p<0.05) with MHA supplementation at 7 days of age. The chymotrypsin activity in pancreas and the dipeptidase activity in small intestinal mucosa and content were not affected (p>0.05) by Met or MHA supplementation.

• The Korean Journal of Physiology and Pharmacology
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• 제18권3호
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• pp.201-209
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• 2014

The present study was designed to investigate the efficacy of selective $ET_A$ receptor antagonist, ambrisentan on hyperhomocysteinemia-induced experimental vascular dementia. L-methionine was administered for 8 weeks to induce hyperhomocysteinemia and associated vascular dementia in male rats. Ambrisentan was administered to L-methionine-treated effect rats for 4 weeks (starting from $5^{th}$ to $8^{th}$ week of L-methionine treatment). On $52^{nd}$ day onward, the animals were exposed to the Morris water maze (MWM) for testing their learning and memory abilities. Vascular endothelial function, serum nitrite/nitrate levels, brain thiobarbituric acid reactive species (TBARS), brain reduced glutathione (GSH) levels, and brain acetylcholinesterase (AChE) activity were also measured. L-methionine-treated animals showed significant learning and memory impairment, endothelial dysfunction, decrease in/serum nitrite/nitrate and brain GSH levels along with an increase in brain TBARS levels and AChE activity. Ambrisentan significantly improved hyperhomocysteinemia-induced impairment of learning, memory, endothelial dysfunction, and changes in various biochemical parameters. These effects were comparable to that of donepezil serving as positive control. It is concluded that ambrisentan, a selective $ET_A$ receptor antagonist may be considered as a potential pharmacological agent for the management of hyperhomocysteinemia-induced vascular dementia.

• BMB Reports
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• 제43권9호
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• pp.622-628
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• 2010

Dimethyl sulfoxide (DMSO) can be reduced to dimethyl sulfide by MsrA, which stereospecifically catalyzes the reduction of methionine-S-sulfoxide to methionine. Our previous study showed that DMSO can competitively inhibit methionine sulfoxide reduction ability of yeast and mammalian MsrA in both in vitro and in vivo, and also act as a non-competitive inhibitor for mammalian MsrB2, specific for the reduction of methionine-R-sulfoxide, with lower inhibition effects. The present study investigated the effects of DMSO on the physiological antioxidant functions of methionine sulfoxide reductases. DMSO elevated hydrogen peroxide-mediated Saccharomyces cerevisiae cell death, whereas it protected human SK-Hep1 cells against oxidative stress. DMSO reduced the protein-carbonyl content in yeast cells in normal conditions, but markedly increased protein-carbonyl accumulation under oxidative stress. Using Msr deletion mutant yeast cells, we demonstrated the DMSO's selective inhibition of the antioxidant function of MsrA in S. cerevisiae, resulting in an increase in oxidative stress-induced cytotoxicity.

• Journal of Microbiology and Biotechnology
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• 제18권1호
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• pp.59-62
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• 2008

The peptide methionine sulfoxide reductases (Msrs) are enzymes that catalyze the reduction of methionine sulfoxide back to methionine. Because of two enantiomers of methionine sulfoxide (S and R forms), this reduction reaction is carried out by two structurally unrelated classes of enzymes, MsrA (E.C. 1.8.4.11) and MsrB (E.C. 1.8.4.12). Whereas MsrA has been well characterized structurally and functionally, little information on MsrB is available. The recombinant MsrB from Bacillus subtilis has been purified and crystallized by the hanging-drop vapor-diffusion method, and the functional and structural features of MsrB have been elucidated. The crystals belong to the trigonal space group P3, with unit-cell parameters a=b=136.096, $c=61.918{\AA}$, and diffracted to $2.5{\AA}$ resolution using a synchrotron-radiation source at Pohang Light Source. The asymmetric unit contains six subunits of MsrB with a crystal volume per protein mass $(V_M)\;of\;3.37{\AA}^3\;Da^{-1}$ and a solvent content of 63.5%.

• 한국가금학회:학술대회논문집
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• 한국가금학회 2001년도 제18차 정기총회 및 학술발표 PROCEEDINGS
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• pp.66-68
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• 2001

An experiment was conducted investigate the of supplemental Cu and Zn methionine chelates on the performance, nutrient digestibility, serum IgG level, gizzard erosion, and Cu and Zn contents in the liver and excretion of broiler chickens. One thousand hatched broiler chickens (Ross) of one day old were assigned to 4 treatments:control(T1), 100ppm of copper in the from of Cu-methionine chelate(Cu-Met, T2), 100ppm of zinc in the from of Zn-methionine chelate(Zn-Met, T3) and 100ppm of copper plus 100ppm of zinc in the from of methionine chelate(Cu-Zn-Met, T4). Each treatment had four replications of 50 bird each. Weight gain of chicks fed chelated products were significantly higher than that of chicks fed control(P＜0.05).Combination of Cu and Zn chelates(Cu-Zn-Met) tended to show the best growth rate and feed conversion ratio. Nutrient digestibilities were not affected by dietary treatments. Serum IgG level of chicks fed Cu-Zn-Met was significantly higher than that of chicks fed control(P＜0.05). Gizzard erosion index was not significantly different among treatments. Contents of Cu and Zn in liver were not significantly affected by dietary treatments, whereas excretions of these minerals were significantly affected by dietary treatments.

• 농업과학연구
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• 제34권2호
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• pp.135-142
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• 2007

본 실험은 사료 첨가제로써 Bromelain과 Zn-methionine의 급여가 착유우의 산유량 및 체세포 수 함량에 미치는 영향을 규명하고자 실시하였다. 홀스타인 착유우 8두(대조구 4두 및 처리구 4두)를 공시하여 대조구는 기본 사료를 급여하고 처리구에서는 Zn-methionine 133g/kg(Zn 20 및 methionine 46.7g)과 bromelain 50g/kg(br 30g/kg)을 경구투여하였다. 그리고 시험은 착유우의 비유초기에 8주간 실시하였다. 본 시험에서 얻은 결과는 다음과 같다. 두당 평균 유량은 대조구(29.6kg)에 비하여 처리구(30.2kg)에서 높게 나타났다(P<0.05). 우유내 체세포수는 대조구($260.8{\times}10^3/ml$)에 비하여 처리구($179.8{\times}10^3/ml$)에서 현저하게 낮게 나타났다(P<0.05). 따라서 이들 첨가물의 급여는 착유우의 산유량을 증가시키고 우유의 위생상 지표가 될 수 있는 체세포수의 감소를 가져오는 결과를 얻었다.