• Korean Journal of Community Nutrition
• /
• v.18 no.1
• /
• pp.45-54
• /
• 2013

This study was conducted to investigate the physical activity pattern, activity coefficient, basal metabolic rate and energy expenditure of female university students. One-day activity diaries were collected from 95 female university students in Seoul. Body composition was measured by Inbody 720. Subjects spent 7 hr 8min on sleeping, 6 hr 31min on studying, 2 hr 50min on physiological activity, 2 hr 3min on leisure, 2 hr 2min on walking and jogging, 1 hr 58 min on commuting and 22min on house chores. The activity coefficient of these subjects was 1.58. The comparison of body composition of subjects according to PAL showed that body weight, body fat mass, arm circumference and arm muscle circumference of physically active group were significantly higher than those of the sedentary group. BMR calculated by Harris-Benedict (H-B) formula and DRI formula and BMR measured by Inbody 720 was 1375 kcal, 1306 kcal and 1209 kcal, respectively. Total energy expenditure (TEE) examined by one-day activity diaries and calculated by H-B formula and estimated energy requirement (EER) formula in DRI was 2102.1 kcal, 2184.4 kcal, and 2164.5 kcal, respectively. The Pearson correlation coefficient between TEE examined by one-day activity diaries and H-B TEE was 0.795 (p < 0.001) while that between TEE examined and DRI EER was 0.604 (p < 0.001). Overall data indicated that female university students seemed to be less active. Therefore it is recommended that universities develop good exercise programs for their students. Further studies are needed to generate more meaningful results with a larger sample size and using machine attached to the body, which are able to detect physical activity more accurately.

• Korean Journal of Adult Nursing
• /
• v.28 no.2
• /
• pp.237-245
• /
• 2016

Purpose: The aim of this study was to examine the effects of an Advanced Practice Nurse (APN)-led self-management program on cardiovascular health status among Korean women at risk of developing or progressing cardiovascular disease. Methods: This pilot study used one-group pre- and post- test experimental design. At health fairs in a community, 30 women who had one or more risk factors for metabolic syndrome were recruited and agreed to participate in the study. A total of 25 women completed the study. The intervention consisted of weekly follow-up calls and self-monitoring diary after an hour of individual counseling regarding risk factors, fast walking, and healthy diet tailored to the participants' needs. Physical activity was assessed with the World Health Organization International Physical Activity Questionnaire and a pedometer. Results: Participants showed statistically significant improvements in blood pressure, body mass index, levels of triglyceride, total cholesterol and low density lipoprotein, numbers of metabolic syndrome factors, and the 10-year CV risk estimate after one month of concentrated intervention. In addition, their physical activity behavior significantly improved after the intervention. Conclusion: This APN-led self-management program targeting modifiable risk factors by offering tailored counseling and concentrated support during the transition might be effective in preventing progression to the cardiovascular disease.

• Journal of Microbiology and Biotechnology
• /
• v.25 no.7
• /
• pp.999-1006
• /
• 2015

The endo-polygalacturonase gene (endo-pgaA) was cloned from DNA of Aspergillus niger SC323 using the cDNA synthesized by overlapping PCR, and successfully expressed in Saccharomyces cerevisiae EBY100 through fusing the α-factor signal peptide of yeast. The fulllength cDNA consists of 1,113 bp and encodes a protein of 370 amino acids with a calculated molecular mass of 38.8 kDa. After induction by galactose for 48 h, the activity of recombinant endo-PgaA in the culture supernatant can reach up to 1,448.48 U/mg. The recombinant protein was purified to homogeneity by ammonium sulfate precipitation and gel filtration column chromatography and subsequently characterized. The optimal pH and temperature of the purified recombinant enzyme were 5.0 and 50℃, respectively. The Michaelis-Menten constant (K_m) and maximal velocity (V_max) of the enzyme for pectin were 88.54 μmol/ml and 175.44 μmol/mg/min, respectively. The enzyme activity was enhanced by Ca²⁺, Cu²⁺, and Na⁺, and strongly inhibited by Pb²⁺ and Mn²⁺. The pectin hydrolysates were mainly galacturonic acid and other oligo-galacturonates. Therefore, these characteristics suggest that the recombinant endo-PgaA may be of potential use in the food and feed industries.

• CELLMED
• /
• v.4 no.1
• /
• pp.7.1-7.8
• /
• 2014

Metabolic effects of ten daily doses of standardized extract of Andrographis paniculata leaves (AP) rich in andrographolide were evaluated in a rat model of type-2 diabetes and in diet induced obese rats. AP was administered per-orally as suspension in 0.3% carboxymethylcellulose at doses of 50, 100 and 200 mg/kg/day for 10 consecutive days. Blood glucose, insulin and lipid profile of rats were measured by using enzyme kits. In addition, effects of such treatments on anti-oxidant enzymes activity and histopathological changes in various organs of diabetic rats were assessed. AP treatments reversed body weight losses and increased plasma insulin level in diabetic rats. The anti-oxidant enzymes activity became normal and histopathological changes observed in pancreas, liver, kidney and spleen of diabetic animals were less severe in extract treated groups. On the other hand, hyperinsulinemia and increased body weight gains observed in high fat or fructose fed rats were less severe in the extract treated groups. These observations revealed therapeutic potentials of the extract for treatments of diabesity associated metabolic disorders, and suggest that the effects of the extract on insulin homeostasis depend on the metabolic status of animals. Activation of cytoprotective mechanisms could be involved in its mode of action.

• BMB Reports
• /
• v.42 no.12
• /
• pp.817-822
• /
• 2009

Type-1 phosphatase (PP-1) was assessed in foot muscle (FM) and hepatopancreas (HP) of estivating (EST) Otala lactea. Snail PP-1 displayed several conserved traits, including sensitivity to inhibitors, substrate affinity, and reduction in size to a 39 kDa catalytic subunit (PP-1c). During EST, PP-1 activity in FM and HP crude extracts was reduced, though kinetics and protein levels of purified PP-1c isoforms were not altered. PP-1c protein levels increased and decreased in nuclear and glycogen-associated fractions, respectively, during EST. Gel filtration determined that a 257 kDa low $K_m$ PP-1$\alpha$ complex decreased during estivation whereas a 76 kDa high $K_m$ complex increased in EST. Western blotting confirmed that the 76 kDa protein consisted of PP-1$\alpha$ and nuclear inhibitor of PP-1 (NIPP-1). A suppression of PP-1 activity factors in the overall metabolic rate depression in estivating snails and the mechanism is mediated through altered cellular localization and interaction with binding partners.

• The Journal of Korean Institute for Practical Engineering Education
• /
• v.2 no.1
• /
• pp.146-153
• /
• 2010

The physics theory applied to the elliptical health trainers can be a good example in engineering education. From the point of view of the physics education the measurement of mechanical and thermal energy expenditure in elliptical trainers can be related to the muscle activity, quantity of motion, and metabolic cost. We realized that the low speed training is effective for high basal metabolism due to increasing the muscle activity even if the high speed training is effective for training down. Elliptical Trainer may provide an effective oxygen exhaustion and thus effective training down. However, the metabolic cost does not have much relation to the amount of training under the high speed of trainer.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.21 no.5
• /
• pp.580-593
• /
• 1992

Cholesterol have many essential functions as a component of cellular and subcellular membranes, metabolic precursor of bile acids and steroid hormones, and obligatory part of the metabolic systems involved in DNA synthesis and cell division. These essential funtions demand a continuous and appropriate supply of cholesterol to the tissues. Body cholesterol pool is maintained by the balance of acquirement from diets, de novo synthesis, and excretion either as bile acids or neutral steroids. In these metabolic process, cholesterol biosynthesis is controlled by the change in the activity of 3-hydroxy-3methylglutaryl coenzyme A (HMG-CoA) reductase. Under most physiological or nutritional situations, the activity of this enzyme is adroitly regulated to maintain tissue cholesterol balance. Excess cholesterol accumulation in the cells induces the decrease in the number of LDL-receptor, followed by the increase in the level of serum LDL-cholesterol. Increase in the level of serum cholesterol appears to be an important determinant for the incidence of the coronary heart disease. Dietary intervention may be helpful in alleviating an increase in the level of serum cholesterol or body cholesterol pool.

• Korean Journal of Veterinary Research
• /
• v.7 no.2
• /
• pp.31-34
• /
• 1967

An experiment was performed in order to investigate the effects of daily oral administration of propylthiouracil per kg body weight on the metabolic rate and the thyroidal activity in ten, Korean native goats by using the Benedict-Roth Metabolism apparatus and the radioactive iodine-131 in the average environmental temperature at $5.7^{\circ}C$. The results obtained were as follows: 1) The mean metabolic rates of the treated group and the control group were $34.5Kcal./m^2/hr.$ and $33.8Kcal./m^2/hr.$ 2) The peak of thyroidal uptake $I^{131}$ rate in the treated group was 20.12% in 48 hrs., following administration of $I^{131}$ and it was 22.62% in the control group in 96 hrs., Highly significant difference was observed(P<0.01).

• Asian Pacific Journal of Cancer Prevention
• /
• v.15 no.22
• /
• pp.10027-10031
• /
• 2014

Background: We investigated the risk of cancer mortality according to obesity status and metabolic health status using sampled cohort data from the National Health Insurance system. Materials and Methods: Data on body mass index and fasting blood glucose in the sampled cohort database (n=363,881) were used to estimate risk of cancer mortality. Data were analyzed using a Cox proportional hazard model (Model 1 was adjusted for age, sex, systolic blood pressure, diastolic blood pressure, total cholesterol level and urinary protein; Model 2 was adjusted for Model 1 plus smoking status, alcohol intake and physical activity). Results: According to the obesity status, the mean hazard ratios were 0.82 [95% confidence interval (CI), 0.75-0.89] and 0.79 (95% CI, 0.72-0.85) for the overweight and obese groups, respectively, compared with the normal weight group. According to the metabolic health status, the mean hazard ratio was 1.26 (95% CI, 1.14-1.40) for the metabolically unhealthy group compared with the metabolically healthy group. The interaction between obesity status and metabolic health status on the risk of cancer mortality was not statistically significant (p=0.31). Conclusions: We found that the risk of cancer mortality decreased according to the obesity status and increased according to the metabolic health status. Given the rise in the rate of metabolic dysfunction, the mortality from cancer is also likely to rise. Treatment strategies targeting metabolic dysfunction may lead to reductions in the risk of death from cancer.

• Journal of Microbiology and Biotechnology
• /
• v.29 no.4
• /
• pp.645-650
• /
• 2019

Brown adipocytes have an important role in the regulation of energy balance through uncoupling protein-1 (UCP-1)-mediated nonshivering thermogenesis. Although brown adipocytes have been highlighted as a new therapeutic target for the treatment of metabolic diseases, such as obesity and type II diabetes in adult humans, the molecular mechanism underlying brown adipogenesis is not fully understood. We recently found that protein tyrosine phosphatase receptor type B (PTPRB) expression dramatically decreased during brown adipogenic differentiation. In this study, we investigated the functional roles of PTPRB and its regulatory mechanism during brown adipocyte differentiation. Ectopic expression of PTPRB led to a reduced brown adipocyte differentiation by suppressing the tyrosine phosphorylation of VEGFR2, whereas a catalytic inactive PTPRB mutant showed no effects on differentiation and phosphorylation. Consistently, the expression of brown adipocyte-related genes, such as UCP-1, $PGC-1{\alpha}$, PRDM16, $PPAR-{\gamma}$, and CIDEA, were significantly inhibited by PTPRB overexpression. Overall, these results suggest that PTPRB functions as a negative regulator of brown adipocyte differentiation through its phosphatase activity-dependent mechanism and may be used as a target protein for the regulation of obesity and type II diabetes.