• International Journal of Advanced Culture Technology
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• 제11권3호
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• pp.190-198
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• 2023

Sentiment analysis can be applied to all texts generated from websites, blogs, messengers, etc. The study fulfills an artificial intelligence sentiment analysis estimating visiting evaluation opinions (reviews) and visitor ratings, and suggests a deep learning model which foretells either an affirmative or a negative inclination for new reviews. This study operates review big data about Jeju tourist attractions which are extracted from Google from October 1st, 2021 to November 30th, 2021. The normalization data used in the propensity prediction modeling of this study were divided into training data and test data at a 7.5:2.5 ratio, and the CNN classification neural network was used for learning. The predictive model of the research indicates an accuracy of approximately 84.72%, which shows that it can upgrade performance in the future as evaluating its error rate and learning precision.

• BMB Reports
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• 제56권6호
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• pp.321-325
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• 2023

RNAs are pivotal molecules acting as messengers of genetic information and regulatory molecules for cellular development and survival. From birth to death, RNAs face constant cellular decision for the precise control of cellular function and activity. Most eukaryotic cells employ conserved machineries for RNA decay including RNA silencing and RNA quality control (RQC). In plants, RQC monitors endogenous RNAs and degrades aberrant and dysfunctional species, whereas RNA silencing promotes RNA degradation to repress the expression of selected endogenous RNAs or exogenous RNA derived from transgenes and virus. Interestingly, emerging evidences have indicated that RQC and RNA silencing interact with each by sharing target RNAs and regulatory components. Such interaction should be tightly organized for proper cellular survival. However, it is still elusive that how each machinery specifically recognizes target RNAs. In this review, we summarize recent advances on RNA silencing and RQC pathway and discuss potential mechanisms underlying the interaction between the two machineries.

• BMB Reports
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• 제56권6호
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• pp.335-340
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• 2023

During normal physiological and abnormal pathophysiological conditions, all cells release membrane vesicles, termed extracellular vesicles (EVs). Growing evidence has revealed that EVs act as important messengers in intercellular communication. EVs play emerging roles in cellular responses and the modulation of immune responses during virus infection. EVs contribute to triggering antiviral responses to restrict virus infection and replication. Conversely, the role of EVs in the facilitation of virus spread and pathogenesis has been widely documented. Depending on the cell of origin, EVs carry effector functions from one cell to the other by horizontal transfer of their bioactive cargoes, including DNA, RNA, proteins, lipids, and metabolites. The diverse constituents of EVs can reflect the altered states of cells or tissues during virus infection, thereby offering a diagnostic readout. The exchanges of cellular and/or viral components by EVs can inform the therapeutic potential of EVs for infectious diseases. This review discusses recent advances of EVs to explore the complex roles of EVs during virus infection and their therapeutic potential, focusing on HIV-1.

• The Korean Journal of Physiology and Pharmacology
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• 제5권4호
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• pp.287-297
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• 2001

Contraction of smooth muscle is initiated by an increase in cytosolic $Ca^{2+}$ leading to activation of $Ca^{2+}$/ calmodulin-dependnet myosin light chain (MLC) kinase and phosphorylation of MLC. The types of contraction and signaling mechanisms mediating contraction differ depending on the region. The involvement of these different mechanisms varies depending on the source of $Ca^{2+}$ and the kinetic of $Ca^{2+}$ mobilization. $Ca^{2+}$ mobilizing agonists stimulate different phospholipases $(PLC-{\beta},\;PLD\;and\;PLA_2)$ to generate one or more $Ca^{2+}$ mobilizing messengers $(IP_3\;and\;AA),$ and diacylglycerol (DAG), an activator of protein kinase C (PKC). The relative contributions of $PLC-{\beta},\;PLA_2$ and PLD to generate second messengers vary greatly between cells and types of contraction. In smooth muscle cell derived form the circular muscle layer of the intestine, preferential hydrolysis of $PIP_2$ and generation of $IP_3$ and $IP_3-dependent\;Ca^{2+}$ release initiate the contraction. In smooth muscle cells derived from longitudinal muscle layer of the intestine, preferential hydrolysis of PC by PLA2, generation of AA and AA-mediated $Ca^{2+}$ influx, cADP ribose formation and $Ca^{2+}-induced\;Ca^{2+}$ release initiate the contraction. Sustained contraction, however, in both cell types is mediated by $Ca^{2+}-independent$ mechanism involving activation of $PKC-{\varepsilon}$ by DAG derived form PLD. A functional linkage between $G_{13},$ RhoA, ROCK, $PKC-{\varepsilon},$ CPI-17 and MLC phosphorylation in sustained contraction has been implicated. Contraction of normal esophageal circular muscle (ESO) in response to acetylcholine (ACh) is linked to $M_2$ muscarinic receptors activating at least three intracellular phospholipases, i.e. phosphatidylcholine-specific phospholipase C (PC-PLC), phospholipase D (PLD) and the high molecular weight (85 kDa) cytosolic phospholipase $A_2\;(cPLA_2)$ to induce phosphatidylcholine (PC) metabolism, production of diacylglycerol (DAG) and arachidonic acid (AA), resulting in activation of a protein kinase C (PKC)-dependent pathway. In contrast, lower esophageal sphincter (LES) contraction induced by maximally effective doses of ACh is mediated by muscarinic $M_3$ receptors, linked to pertussis toxin-insensitive GTP-binding proteins of the $G_{q/11}$ type. They activate phospholipase C, which hydrolyzes phosphatidylinositol bisphosphate $(PIP_2),$ producing inositol 1, 4, 5-trisphosphate $(IP_3)$ and DAG. $IP_3$ causes release of intracellular $Ca^{2+}$ and formation of a $Ca^{2+}$-calmodulin complex, resulting in activation of myosin light chain kinase and contraction through a calmodulin-dependent pathway.

• 한국산학기술학회논문지
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• 제21권8호
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• pp.195-204
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• 2020

본 연구는 20대 성인의 스마트폰 의존 관련 요인을 확인하는 것을 목적으로 한다. 본 연구를 위하여 2017년 과학기술정보통신부와 한국정보화진흥원에서 실시한 스마트폰 과의존 실태조사 중 20대, 3,684명의 자료를 분석하였다. 자료분석은 빈도와 백분율, 평균과 표준편차, 독립표본 T 검증, Pearson 상관관계, 가중치를 적용한 위계적 다중 회귀분석을 사용하였다. 그 결과, 대상자들은 스마트폰 의존도가 증가할수록 스마트폰 사용시간(β=.18, p=.000)과 빈도(β=.04, p=.000), 게임(β=.15, p=.000), 영상시청(β=.09, p=.000), 모바일 쇼핑(β=.05, p=.000), 업무(β=.05, p=.010), 이메일(β=.13, p=.000), 스포츠 배팅(β=.07, p=.000)이 유의하게 증가하였다. 반면, 스마트폰으로 음악을 듣는 것(β=-.07, p=.000), 성인물 사용(β=-.07, p=.000)은 스마트폰 의존을 감소시키는 요인이었고, SNS(Social Networking Services)(β=.01, p=.358)와 메신저(β=-.02, p=.330)는 스마트폰 의존도의 유의한 관련 요인이 아니었다. 그러나 메신저는 대상자들이 가장 많이 사용하는 앱 유형이었고, 업무와 강한 상관관계를 가졌다(r=.55, p=.000). 본 연구를 통하여 20대 성인의 스마트폰 의존과 관련된 스마트폰 사용행태가 아동이나 청소년과 차이가 있음이 확인되었다. 이러한 결과는 20대 성인의 스마트폰 의존을 올바로 이해하고, 의존 조기발견과 예방 및 관리에 활용할 수 있을 것이다.

• 한국정보통신학회논문지
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• 제10권1호
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• pp.142-147
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• 2006

컴퓨터와 네트워크의 보급이 일반화되면서 인터넷을 통한 정보 전달이 일상생활처럼 되고 있다. 기존에는 정보를 전달하기 위한 방법이 주로 전자메일에 한정되어 있던 것에 반해, 요즘은 좀 더 즉각적으로 메시지를 전달해주는 인스턴트 메신저를 많이 사용하고 있다. 인스턴트 메신저는 이러한 장점으로 인해 국내에서도 사용자가 많이 늘고 있다. 그러나 대부분의 인스턴트 메신저 서비스는 인터넷상에서 많은 부분이 노출되지만 클라이언트는 이를 느끼지 못한 채 사용하고 있다. 이는 마치 전화도청과 같다고 할 수 있다. PKI를 사용한 암호화 기술은 인테넷에서 접근 통제, 인증, 기밀성, 무결성, 부인거절 등의 서비스들을 제공할 수 있는 공개키 기반 구조를 발달시켜 왔다. 본 논문에서는 인스턴트 메신저의 안전한 통신을 위해 PKI(공개키 기반구조)를 이용한 인스턴트 메신저에서의 인증 프로토콜에 대해 설계하였다.

• BMB Reports
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• 제32권3호
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• pp.215-225
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• 1999

Phytochromes (with gene family members phyA, B, C, D, and E) are a wavelength-dependent light sensor or switch for gene regulation that underscore a number of photo responsive developmental and morphogenic processes in plants. Recently, phytochrome-like pigment proteins have also been discovered in prokaryotes, possibly functioning as an auto-phosphorylating/phosphate-relaying two-component signaling system (Yeh et al., 1997). Phytochromes are photochromically convertible between the light sensing Pr and regulatory active Pfr forms. Red light converts Pr to Pfr, the latter having a "switch-on" conformation. The Pfr form triggers signal transduction pathways to the downstream responses including the expression of photosynthetic and other growth-regulating genes. The components involved in and the molecular mechanisms of the light signal transduction pathways are largely unknown, although G-proteins, protein kinases, and secondary messengers such as $Ca^{2+}$ ions and cGMP are implicated. The 124-127 kDa phytochromes form homodimeric structures. The N-terminal half contains the tetrapyrrolic phytochromobilin for red/far-red light absorption. The C-terminal half includes both a dimerization motif and regulatory box where the red light signal perceived by the chromophore-domain is recognized and transduced to initiate the signal transduction cascade. A working model for the inter-domain signal communication within the phytochrome molecule is proposed in this Review.

• Journal of Information Technology Applications and Management
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• 제16권2호
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• pp.45-63
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• 2009

China has the largest number of the user of web 2.0 such as messengers and blogs, as of 2008 year. Chinese Internet digital market will grow from $85 billion in 2007 to $264 billion in 2015. The importance of Chinese Internet business is increasing, because of its size and growth potential. This research is focused on following two questions. First, what are the major characteristics of social networking services in China? In order to find out the answer about this question, the case study on Tencent QQ and Sina Poco, the most representative social networking services company in China, was done. And the reason of the use of social networking services, their contents, and the attitude toward the paid service were identified. Second, what are the major factors influencing the use of social networking services in China. User participation, network effect, usefulness, and system quality were inferred as the factors influencing the use. These factors were based on motivation theory, technology acceptance model, and the related research papers. This research model was proved by data analysis using Partial Least Square. As results, the use of social networking services was influenced by usefulness, network effect, user participation, and system quality. But social influence was not significant of the use of social networking services.

• Current Research on Agriculture and Life Sciences
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• 제32권1호
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• pp.43-52
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• 2014

Reactive oxygen and nitrogen species (ROS and RNS, respectively) are messengers that carry signals to alter the redox state in order to activate plant responses and other physiological processes, such as differentiation, aging, senescence, and pathogen defense. Quite a large number of genes are involved in this signaling and lead to oxidative stress in plants. Although the role of ROS/RNS during stress conditions is well documented, a comprehensive list of genes and comparative study of these genes has not yet been completed. Accordingly, the in silico identification of oxidative stress-related genes was performed for soybeans and Arabidopsis. These genes were also studied in relation to multiple domain prediction. The presence of domains like dehydogenase and ATPase suggests that these genes are involved in various metabolic processes, as well as the transportation of ions under optimal environmental conditions. In addition to a sequence analysis, a phylogenetic analysis was also performed to identify orthologous pairs among the soybean and Arabidopsis oxidative stress-related genes based on neighbor joining. This study was also conducted with the objective of further understanding the complex molecular signaling mechanism in plants under various stress conditions.

• 패션비즈니스
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• 제21권3호
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• pp.67-79
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• 2017

This study investigated consumers' enjoyment, perceived risks, expected values, and innovativeness factors and the effects of the convenience and personalization of "online to offline" (O2O) fashion mobile commerce on its perceived usefulness, perceived ease of use, and consumers' attitudes and intention to use O2O mobile commerce. A research model was developed using the Technology Acceptance Model (TAM). A mobile survey was conducted through smartphone messengers and SNSs targeting male and female college students in their 20s who are living in the Seoul Metropolitan Area. A total of 192 questionnaire responses were used in the analysis. "Among the consumer characteristics, only enjoyment and expected values were found to make consumers feel that the O2O fashion mobile commerce is useful and easy to use. Among the mobile commerce characteristics, only convenience was found to have significant effects on consumers' perceived usefulness and ease of use regarding O2O fashion mobile commerce. Perceived usefulness was found to have the effects on attitudes as well as intention to use toward O2O mobile commerce. It was shown that positive attitudes toward O2O mobile commerce led to positive use intention toward O2O mobile commerce.