• International Journal of Oral Biology
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• v.39 no.1
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• pp.35-40
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• 2014

Halitosis is caused by consumption of certain foods or drinks and production of volatile sulfur compounds (VSCs) by periodontopathogens. VSCs-related halitosis is not easily removed using mechanical or chemical therapies such as dental floss, plaque control and mouth rinse. Lactobacillus are known to be probiotics and stimulate immune systems of human. Furthermore, L. casei ATCC 334 and L. rhamnosus GG have an effect on protection of dental caries in vitro studies. The aim of this study was to investigate effect of Lactobacillus on halitosis by Fusobacterium nucleatum- and Porphyromonas gingivalis-producing VSCs and to analyze inhibitory mechanism. The periodontopathogens were cultivated in the presence or the absence Lactobacillus, and the level of VSCs was measured by gas chromatograph. For analysis of inhibitory mechanisms, the susceptibility assay of the spent culture medium of Lactobacillus against F. nucleatum and P. gingivalis was investigated. Also, the spent culture medium of Lactobacillus and periodontopathogens were mixed, and the emission of VSCs from the spent culture medium was measured by gas chromatograph. L. casei and L. rhamnosus significantly reduced production of VSCs. L. casei and L. rhamnosus exhibited strong antibacterial activity against F. nucleatum and P. gingivalis. The spent culture medium of L. casei inhibited to emit gaseous hydrogen sulfide, methyl mercaptan and dimethyl sulfide from the spent culture medium of periodontopathogens. However, the spent medium of L. rhamnosus repressed only dimethyl sulfide. L. casei ATCC 334 may improve halitosis by growth inhibition of periodontopathogens and reduction of VSCs emission.

• Journal of Integrative Natural Science
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• v.8 no.2
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• pp.128-134
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• 2015

This paper focuses on the applicability of a continuous monitoring method on trace sulfur compounds in the ambient air by TD and GC/PFPD. The target compounds for monitoring include H2S(hydrogen sulfide), Methyl mercaptan, Dimethyl Sulfide, and Dimethyl disulfide. The result of QA/QC on monitoring instruments satisfies all the standards of Odor Measurement and Analysis Method, showing that the reproductivity of the compounds by concentration is within 10%, linearity is above 0.98 of a correlation efficient, method detection limit is 0.16 ppb by MM standard, and recovery rate is over 70%. Monitoring was conducted for two years from March 2006 to February 2008. As a result of the monitoring, the average concentration of H2S was 0.08 ppb, with the maximum concentration at 16.15 ppb. The result indicates that it is reasonable to do continuous monitoring as there appears a spontaneous event of high concentration by the condition of the site during monitoring odor-causing substances. Therefore, it is suggested that the continuous monitoring method used in this paper is appropriate to identify the characteristics of sudden occurrence and concentration variations of sulfur compounds.

• Fisheries and Aquatic Sciences
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• v.23 no.9
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• pp.21.1-21.10
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• 2020

Background: This study aimed to provide a basic standard for assessing freshness and acceptability of whiteleg shrimp (Litopenaeus vannamei). Methods: It was divided into whole body and meat and stored at 25 ℃ to evaluate both quality and freshness changes that occur over time. The shelf life of shrimp was estimated as 17 and 20 h for whole body and meat, respectively. Results: In chemical analysis, K-value increased from 9.96 to 12.32% to a maximum of 75.14%, and TVB-N increased from 1.86 mg/100 g to 34.71 mg/100 g. For volatile sulfur compounds, methyl mercaptan and dimethyl disulfide increased from 0.00 mg/100 g to 1.10 mg/100 g and 1.26 mg/100 g, respectively, rapidly increasing with decreasing freshness. Conclusion: Changes in all biochemical indicators significantly correlated with the sensory evaluation results. This study contributes to the knowledge about whiteleg shrimp spoilage and freshness, providing a basis for developing methods to improve shrimp quality control and management.

• Journal of Korean Society of Water and Wastewater
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• v.27 no.1
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• pp.59-67
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• 2013

The odor occurring in the sewage system induces the displeasure, the disgust such as the headache, the vomit, etc. and increases the spiritual stress and disturbs the pleasant life of residents. These odors occur mainly in the area of combined sewage system treatment, being created in the personal sewage treatment plant such as septic tank and are incoming to sewage pipes and emitted to the outside through the manhole and the receiver, etc.; and this causes odors to the people. The Hydrogen Sulfide, the Methyl Mercaptan, the Ammonia, etc. are materials causing the odor, the more serious issue of odor is occurring since the septic tank of degradation process is being applied. The primary cause of odor is the decomposition of human feces in the septic tanks and sewage disposal facilities. The purpose of this study is reduction of hydrogen sulfide using air supplying and SOB(Sulfur-Oxidizing Bacteria). As a result of this study of the air supply system and the SOB media equipment by air supply, in case the air is injected to SOB media compared to the injection of air only, the removal efficiency the hydrogen sulfide was average 3.4 times higher.

• Proceedings of the Korea Technical Association of the Pulp and Paper Industry Conference
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• 2006.06b
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• pp.317-321
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• 2006

The investigation of the formation of organic sulfur compounds (i.e., odor species), mainly methyl mercaptan and demethyl suifide, during kraft pulping has been conducted, in which both hardwood and softwood species were used. It was discovered that there is an organic sulfur compounds formation phase transition point with respect to delignification extent. The transition point occurs at a kappa number of approximately 35 and 20 for softwoods and hardwoods, respectively. The results also showed that both low sulfidity and anthraquinone (AQ) addition are helpful to control the formation of theses organic sulfur compounds during kraft pulping. Whereas Hexenuronic acid (HexA) has contribution to Kappa number, the extended delignification not only lead to low pulp yield, but also be adverse to odor control. A significant in-digester odor reduction can be achieved if the pulping is to be terminated before phase transition point and combined with AQ addition.

• Journal of the Korean Society of Food Culture
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• v.25 no.5
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• pp.607-614
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• 2010

Changes in major volatile odor components (VOC) and sensory properties of kimchi during ripening for 4 days were investigated, and major VOCs of the raw materials of kimchi were also analyzed. Seven of eight major VOCs of kimchi originated mainly from garlic, while one originated from ginger. During 4 days of kimchi ripening, the amount of ethanol, which was substantially higher than that of other VOCs, increased continuously but decreased slightly on the fourth day. The amount of diallyl disulfide decreased during ripening, while that of allyl mercaptan decreased on the first day and increased slightly thereafter. The amount of methyl allyl sulfide, diallyl sulfide, and methyl trisulfide increased continuously during ripening, while that of dimethyl disulfide and methyl propyl disulfide increased until the second day and decreased thereafter. Scores of overall acceptability, taste, and odor for kimchi ripened for 2 days were significantly higher than those of other samples (p<0.05). The correlation between scores of overall acceptability and the amount of dimethyl disulfide or methyl propyl disulfide was higher than that of other VOCs.

• 한국신재생에너지학회:학술대회논문집
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• 2010.11a
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• pp.152.1-152.1
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• 2010

매립지에서 유기물의 분해로 발생되는 매립가스는 악취 등으로 인한 대기오염뿐만 아니라 온난화지수가 21인 메탄이 약 50vol% 이상 포함되어 있어 지구온난화에 큰 영향을 미친다. 하지만 매립가스를 에너지원으로 활용하면 대기오염저감, 지구온난화 감소, 대체에너지원 확보뿐만 아니라 CDM사업 등과 연계하여 부가수익창출이 가능하다. 현재 국내에는 약 242개의 폐기물매립지가 있는데, 이중 매립가스를 활용하는 곳은 단지 14개소로 개별 경제성이 있는 대형매립지에서만 자원화시설을 설치하여 운영 중이며 그 외 매립지에서는 매립가스를 소각 또는 단순 대기 방출하여 대기오염유발과 동시에 대체에너지원 미활용으로 국가차원에서 큰 손실이므로 이를 활용할 수 있는 기술개발이 시급하다. 현재 매립가스 에너지화 기술로는 매립가스 열량에 따라 가스엔진, 가스터진, 증기터빈을 이용하는데 국내에서는 수분제거와 같은 간단한 처리 과정을 거친 후, 정제 없이 사용한다. 그런데 매립가스 구성 성분 중 일부 미량가스($H_2S$ 등)는 부식성이 높아 실제 공정에서 큰 문제점으로 작용하게 되므로 전처리공정이 반드시 필요하다. 본 연구에서는 중소규모 매립지에서 발생하는 매립가스를 중심적환장으로 이송하여 경제성을 가지는 에너지원으로 활용할 수 있는 기술개발을 목표로 하이드레이트 기술을 활용함에 있어 전처리 기초연구를 수행하였다. 매립가스 구성성분 중 대표적 악취물질인 메르캅탄과 부식성 물질인 황화수소의 전처리 기술로서 활성탄 흡착방법을 이용하여 외부에서 관찰이 가능하고 흡착탑을 2단으로 구성하여 활성탄 흡착탑을 제작하였다. 대상가스는 일반적으로 매립가스에 포함되어 있는 성분으로 제작하여 사용하였고 흡착탑 전 후 가스의 성분분석은 LMSxi를 이용하였다. 실험결과 활성탄의 상태, 접촉시간, 흡착탑의 구성에 따라 50~80%의 제거효율을 보였으며 이는 활성탄 흡착탑을 매립가스 에너지화의 전처리 시설로 사용될 경우 각각의 변수들에 대해 정확한 공정설계가 필요하다고 할 수 있다.

• The Journal of Internal Korean Medicine
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• v.35 no.4
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• pp.532-545
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• 2014

Objectives: The purpose of this study was to evaluate the clinical effect of Korean medical treatments such as herbal medicine, acupuncture, and electroacupuncture by using the portable gas chromatograph OralChroma$^{TM}$ (Abimedical, Japan) in halitosis patients. Methods: We surveyed 30 halitosis patients who had visited the Oral Diseases Clinic in the Korean Medical Hospital of Kyunghee University from October, 2013 to November, 2014. Before starting Korean medical treatment, the subjects were evaluated on sociodemographic characteristics, severity of discomfort using visual analogue scale (VAS), unstimulated salivary flow rate (USFR), and halitosis associated life-quality test (HALT) score. To evaluate the therapeutic effect, we measured the volatile sulfur compounds (VSCs) in breath by using OralChroma$^{TM}$ before and after 3 weeks treatment. Results: The concentration of total VSCs measured by OralChroma$^{TM}$ significantly decreased (p=0.001). Furthermore, the level of hydrogen sulfide and methyl mercaptan also significantly decreased (p<0.05). However, although the level of dimethyl sulfide decreased as well, there was no significance(p>0.05). Conclusions: Korean medical treatment was effective in treating halitosis by decreasing VSCs. Further study, with well-designed randomized controlled trials with larger number of cases will be needed in the future.

• Journal of Environmental Health Sciences
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• v.41 no.5
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• pp.327-334
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• 2015

Objectives: In order to review both the effects of sewage sludge dewaterability and deodorizing ability in the existing advanced sewage treatment process, BIO-CLOD (a solidified mixture of 10% Bacillus concentrate and inorganics) was used as an inorganic conditioner effective for deodorization treatment in addition to the removal of organic matter and nutritive salts. Methods: Sludge dewaterability was evaluated using specific resistance to filtration (SRF) experiments with six agitators and two types of commercially available BIO-CLOD; one that is made by grinding solid matter (powdered BIO-CLOD) and one that has been obtained by sieving a separation with a particle diameter of 100 mesh (100 mesh BIO-CLOD). For deodorization odor treatment experiments, 20 g of commercially available solid BIO-CLOD was submerged in four liters of sewage sludge and mixed and agitated for 45 days. Results: When BIO-CLOD was injected into sewage sludge to experimentally compare specific resistance to filtration (SRF), the optimum amount of BIO-CLOD to be injected was shown to be 2% w/v and the SRF value in this case was $1.35{\times}10^{12}m/kg$. pH changes following BIO-CLOD injection were within 6.5-7.0. By 14 days after submerging BIO-CLOD into the sewage sludge to evaluate its deodorizing ability, $H_2S$ decreased by 68% and methyl mercaptan decreased by 74%. By 45 days after the submergal, both items decreased by 100%, indicating deodorizing ability. Conclusion: To compare the levels of dewaterablity of sewage sludge at different particle sizes of inorganic conditioner, powdered BIO-CLOD, particle size 100 mesh BIO-CLOD, and bentonite were tested. It could be seen that as the powdered BIO-CLOD increased, the precipitability increased up to 62% in 30 minutes. As an inorganic conditioner, BIO-CLOD was identified as a stable sludge conditioner that does not affect pH.

• Journal of Korean Society for Atmospheric Environment
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• v.23 no.1
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• pp.39-49
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• 2007

In this study, the performance characteristics of solid phase microextraction (SPME) were investigated for three major odorous groups that consist of 10 individual compounds ([1] volatile organic compounds (VOC): benzene, toluene, p-xylene and styrene, [2] reduced sulfur compounds (RSC): hydrogen sulfide, methyl mercaptan, dimethylsulfide (DMS), dimethyldisulfide (DMDS), and carbon disulfide, and [3] amine: trimethylamine (TMA)). For the purpose of a comparative analysis, two types of SPME fiber ([1] polidimethylsiloxane/divinilbenzene (P/D) and [2] $Carboxen^{TM}$/polidimethylsiloxane (C/P)) were test ε d against each other for a series of standards prepared at different concentration levels (100, 200, and 500 ppb). To compare the analytical performance of each fiber, all standards were analyzed for the acquisition of calibration data sets for each compound. The results of P/D fiber generally showed that its calibration slope increased as a function of molecular weight across different VOCs; however, those of C/P fiber showed a fairly reversed trend. Besides, we confirmed that the application of SPME is limited to many sulfur compounds; only two compounds (DMS and DMDS) are sensitive enough to draw calibration results out of SPME. The calibration data for RSC show generally enhanced slop values for C/P relative to P/D fiber. However, in the case of TMA, we were not able to find a notable difference in their performance.