• 제목/요약/키워드: membrane potential

검색결과 1,520건 처리시간 0.028초

The Succinate : Quinone Oxidoreductase of Marine Bacterium Vibiro alginolyticus is a $H^+$

  • Kim, Young-Jae
    • Journal of Microbiology and Biotechnology
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    • 제10권1호
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    • pp.48-50
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    • 2000
  • The energetics at the succinate:quinone oxidoreductase segment of V. alginolyticus was studied using a fluorescence quenching technique with inside-out membrane vesicles. A transient generation of the membrane potential (inside-positive) and ${\Delta}pH$ (inside-acidic) occurred in the presence of KCN and succinate when ubiquinone-1 (Q1) was added. The membrane potential (\Delta\psi$) generated by the succinate; quinone oxidoreductase segment was completely collapsed by the protonophore carbonylcyanide m-chlorophenylhydrazone (CCCP) and the membrane permeable anion $SCN^{-}$, whereas the ${\Delta}pH$ was completely collapsed by CCCP and $(NH_4)_2SO_4$. From these results, it was concluded that the succinate: quinone oxidoreductase segment as well as quinol oxidase [1] in the respiratory chain of V. alginolyticus generated $H^{+}$ electrochemical potential.

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Energy Status of Neurospora crassa Mutant nap in Relation to Accumulation of Carotenoids

  • Belozersk, Tatyana A.;Potapova, Tatyana V.;Isakova, Elena P.;Shurubor, Eugene I.;Savel'eva, Ludmila V.;Zvyagilskaya, Renata A.
    • Journal of Microbiology
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    • 제41권1호
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    • pp.41-45
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    • 2003
  • N crassa mutant strain nap showed reduced growth rate, decreased electric membrane potential, and elevated intracellular ATP content in comparison to the wild type. Blue light induced a hyperpolarization of the membrane potential in both strains. The analysis of oxidative and phosphorylation activities of mitochondria isolated from the two strains has revealed that nap utilized more efficient oxidative pathways. The higher intracellular ATP content in the nap was presumably due to impaired transport systems of the plasma membrane, and to a lesser extent to the functioning of the fully competent respiratory chain. The excess ATP possibly accounts for carotenoid accumulation in the mutant.

Up-regulation of Cyelin A-Cdk2 activity is associated with depolarization of mitochondrial membrane potential during apoptosis of human hepatoma SK-HEP1 cells induced by treatment with panaxadiol

  • Park, Byoung-Duck;Jin, Ying-Hua;Yim, Hyung-Shin;Lee, Seung-Ki
    • 대한약학회:학술대회논문집
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    • 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2-2
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    • pp.167.1-167.1
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    • 2003
  • Here we show that panaxadiol, a ginseng saponin with a dammarane skeleton, induces acute apoptotic cell death in human hepatoma SK-HEP-1 cells as evidenced by analysis of DNA fragmentation, caspase activation, and changes in cell morphology. The kinetic study showed that panaxadiol-induced apoptosis is associated with depolarization of mitochondrial membrane potential and cytochrome c release. Sequential activations of caspases-depolarization of mitochondrial membrane potential and cytochrome c release. Sequential activations of caspases-9, and -3, or -7, but not of caspase 8 coincide well in a time dependent manner with mitochondrial membrane depolarization and cytochrome c release from mitochondria during apoptosis of SK-HEP-1 cells induced by treatment with panaxadiol. (omitted)

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Effects of EPS on membrane fouling in a hybrid membrane bioreactor for municipal wastewater treatment

  • Zhang, Aining;Liu, Zhe;Chen, Yiping;Kuschk, Peter;Liu, Yongjun
    • Membrane and Water Treatment
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    • 제5권1호
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    • pp.1-14
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    • 2014
  • A pilot-scale hybrid membrane bioreactor (HMBR) for real municipal wastewater treatment was developed by adding biofilm carriers into a conventional membrane bioreactor, distribution and dynamic changes of the extracellular polymeric substances (EPS) and their roles in membrane fouling were investigated. The results showed that the concentrations of loosely bond EPS (LB-EPS) and tightly bond EPS (TB-EPS) in activated sludge, carrier biofilm and sludge cake layer have been increased significantly with the running time of HMBR, during operation of the HMBR, EPS demonstrated positive correlations with membrane fouling. Compared to TB-EPS, LB-EPS showed more significant correlations with sludge physical properties and specific resistance to filtration (SRF) in HMBR, and thus demonstrated that LP-EPS have a stronger potential of fouling than TB-EPS. It was also found that a lower organic loading in HMBR could result a significant increase in EPS concentration, which would in turn influence membrane fouling in HMBR. This critical investigation would contribute towards a better understanding of the behavior, composition and fouling potential of EPS in HMBR operation.

해수담수화 공정에서 역삼투막의 거동에 영향을 주는 요인 (Factors related to Performance of Reverse Osmosis Membrane in Seawater Desalination Process)

  • 박준영;홍성호;김지훈;정우원;남종우;김영훈;이창하;김형수
    • 상하수도학회지
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    • 제25권2호
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    • pp.171-176
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    • 2011
  • Organic matters that comprise a tiny part of seawater generally occur over 50% of membrane fouling in Reverse Osmosis Process. This study evaluates Foundation efficiency of reverse osmosis membranes under brackish and seawater conditions and resistance of organic fouling. Moreover, analyzing the membrane surface through roughness, contact angle and zeta potential results in roughness and contact angle are proportional to flux decline rate (FDR), yet FDR has high value when zeta potential is low level. Furthermore, with various membrane fouling of different raw water conditions, the flux tends to improve when pH value is high and raw water which is complex with organic and cation pollutes membrane faster than organic separated raw water condition.

Non Stationary Ionic Current through Polymer Charged Membrane

  • Jeong, Sung-Hyun;Lee, Won-Chul;Yang, Wong-Kang
    • Bulletin of the Korean Chemical Society
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    • 제24권7호
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    • pp.937-942
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    • 2003
  • The relaxation phenomena of ionic currents through the charged membrane under the constant applied potentials has been studied. The formulation was obtained for the non stationary current by assuming that the ion mobility is independent of concentration and the potential gradient is a constant within membrane, and it was applied to the experimental results with the sulfonated polystyrene collodion base membrane. It has been shown that the initial ion distributions in the membrane play a predominant role in the relaxation phenomena.

Therapeutic Potential of Membrane Fatty Acid Modifiaction in Tumor Cells

  • Shon, Yun-Hee;Park, Kun-Young;Kim, Kwang-Soo
    • Preventive Nutrition and Food Science
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    • 제1권1호
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    • pp.143-150
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    • 1996
  • The membrane fatty acid composition of tumor cell can be modified either in cell by altering the lipid composition of the medium of during growth in animals by changing the dietaty fat composition. These modifications are associated with changes in membrane physical properties and certain cellular functions, including carrier-mediated transport and enzyme contained within the membrane. Such effects influence the transport of nutrients and chemotherapeutic agents in cancer cells .Fatty acid modification also can enhance the sensitivity of the neoplastic cell to chemotherapy. The alteration in plasma membrane composition will be affected through dietary supplementations and the potential value to cancer patients could be a better understanding of the effects of diet on responsiveness of neoplasms to chemotherapy, i.e. cancer patients' chances for a "cure" can be improved by diet changes prior to treatment.

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Reference Electrode at Molten Salt: A Comparative Analysis of Electroceramic Membranes

  • Yoon, Seokjoo;Kang, Dokyu;Sohn, Sungjune;Park, Jaeyeong;Lee, Minho;Choi, Sungyeol
    • 방사성폐기물학회지
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    • 제18권2호
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    • pp.143-155
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    • 2020
  • A reference electrode is important for controlling electrochemical reactions. Evaluating properties such as the reduction potential of the elements is necessary to optimize the electrochemical processes in pyroprocessing, especially in a multicomponent environment. In molten chloride systems, which are widely used in pyroprocessing, a reference electrode is made by enclosing the silver wire and molten salt solution containing silver chloride into the membranes. However, owing to the high temperature of the molten salt, the choice of the membrane for the reference electrode is limited. In this study, three types of electroceramic, mullite, Pyrex, and quartz, were compared as reference electrode membranes. They are widely used in molten salt electrochemical processes. The potential measurements between the two reference electrode systems showed that the mullite membrane has potential deviations of approximately 50 mV or less at temperatures higher than 650℃, Pyrex at temperatures lower than 500℃, and quartz at temperatures higher than 800℃. Cyclic voltammograms with different membranes showed a significant potential shift when different membranes were utilized. This research demonstrated the uncertainties of potential measurement by a single membrane and the potential shift that occurs because of the use of different membranes.

Reuse potential of spent RO membrane for NF and UF process

  • Ng, Zhi Chien;Chong, Chun Yew;Sunarya, Muhammad Hamdan;Lau, Woei Jye;Liang, Yong Yeow;Fong, See Yin;Ismail, Ahmad Fauzi
    • Membrane and Water Treatment
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    • 제11권5호
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    • pp.323-331
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    • 2020
  • With the increasing demand on reverse osmosis (RO) membranes for water purification worldwide, the number of disposed membrane elements is expected to increase accordingly. Thus, recycling and reuse of end-of-life RO membranes should be a global environmental action. In this work, we aim to reuse the spent RO membrane for nanofiltration (NF) and ultrafiltration (UF) process by subjecting the spent membrane to solvent and oxidizing solution treatment, respectively. Our results showed that solvent-treated RO membrane could perform as good as commercial NF membrane by achieving similar separation efficiencies, but with reduced water permeability due to membrane surface fouling. By degrading the polyamide layer of RO membrane, the transformed membrane could achieve high water permeability (85.6 L/㎡.h.bar) and excellent rejection against macromolecules (at least 87.4%), suggesting its reuse potential as UF membrane. More importantly, our findings showed that in-situ transformation on the spent RO membrane using solvent and oxidizing solution could be safely conducted as the properties of the entire spiral wound element did not show significant changes upon prolonged exposure of these two solutions. Our findings are important to open up new possibilities for the discarded RO membranes for reuse in NF and UF process, prolonging the lifespan of spent membranes and promoting the sustainability of the membrane process.

캐리어 막에 의한 알카리 금속 이온의 업-힐 수송의 특성 연구 (Study of Characteristic of Up-hill Transport in Alkali Metal-ions Through a Carrier Membrane)

  • 박근덕;양원강
    • 분석과학
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    • 제12권3호
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    • pp.224-229
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    • 1999
  • 최근 캐리어를 이용한 액체막의 연구가 대단히 활발하다. 항생물질의 모넨신을 사용하여 옥타놀의 유기용매에 용해시켜 양이온교환막을 제조하였다. 이막의 평가를 Nernst-Planck식과 Fick식으로 막전위와 물질의 투과선택도를 분석하였다. 사용한 알카리이온의 농도에 대한 높은 투과선택성을 확인하였고, 시간에 따른 이온농도값은 선형관계를 알았다. 그러나 이온의 높은농도에서 변화량은 선형이 아니였다. 이를 위한 해석은 정지층(Stagnent Layer)을 하나 더 도입하여 업 - 힐 수송에서 직선관계를 얻었다.

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