• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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• pp.318.2-318.2
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• 2002

The effects of oxidative stress on the alterations of different antioxidant enzyme activity on mouse melanoma cells(B16F10) was investigated. Oxidative stress was induced by the exposeure to hydrogen peroxide(H2O2). B 16F 10 cells were exposed Phellinus linteus Ex. in combination with H2O2 and measured the time course of changes in cell viability and antioxidant enzyme activity. CAT activity peaked at 12 hr. On the contrary, SOD and GPX activity was maximum at 6 hr. The cell viability of Pheltinus linteus extracts in combination with hydrogen peroxide was higher than hydrogen peroxide alone.

• Natural Product Sciences
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• 제17권2호
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• pp.135-141
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• 2011

Celastrus orbiculatus has been widely used as a traditional medicine for the treatment of many diseases including rheumatoid arthritis and odontalgia. In the present study, anti-metastatic activity of a methanolic extract from C. orbiculatus (MCO) was studied. A gelatin zymographic assay revealed that MCO has potent inhibitory effects on MMP-2 and MMP-9 activities in B16F10 melanoma cells. Moreover, MCO attenuated MMP expression via down-regulation of NF-${\kappa}$B translocation to the nucleus. Melanoma cell migration and invasion were also down-regulated by MCO. In addition, MCO significantly suppressed lung metastasis in an in vivo model. These results strongly suggest that MCO may possibly be used as a valuable anti-metastatic agent for cancer treatment.

• KSBB Journal
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• 제32권3호
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• pp.218-233
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• 2017

To identify the natural and novel cosmeceutical ingredients which have whitening function, we examined the effects of tyrosinase activity and melanin production on halophyte Limonium tetragonum (Thunb.) A. A. Bullock extracts. As a result, the extracts showed whitening effect with no cytotoxicity. Melanin contents of B16F10 melanoma cells were decreased in a dose-dependent manner at 50, 100, $200{\mu}g/mL$ treatment of the extracts. In tyrosinase activity inhibition test, L. tetragonum (Thunb.) A. A. Bullock extracts showed decreased tyrosinase activity as the concentration of ${\alpha}-MSH$ was increased. Furthermore, it was observed that the tyrosinase and MITF expression was significantly downregulated by adding L. tetragonum (Thunb.) A. A. Bullock extracts in ${\alpha}-MSH$, a melanogenesis inducing material, treated B16F10 melanoma cells. These results indicate that L. tetragonum (Thunb.) A. A. Bullock extract might be an effective whitening agent by inhibit melanin formation.

• Journal of Acupuncture Research
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• 제21권6호
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• pp.63-84
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• 2004

Objective & Methods : To study the effects of anti-cancer, anti-metastasis and immune response improvement of herbal-acupuncture with Eclipta prostrata diffusae herba infusion solution(ELP-HAS), we injected ELP-HAS into Chung-wan(CV12) of C57BL/6 mice implanted intravenously with B16-F10 melanoma. We have reached the following conclusion through the effect on the percentage of $CD25^+/CD4^+$, $CD8^+/CD3e^+$, $CD69^+/B220^+$, $NK1.1^+/CD3e^+$cells in mouse PBMCs, the effect on the pulmonary colony formation number, and the effect on MST(Median Survival Time) and ILS(Increase of Life Span) of C57BL/6 mice implanted intravenously with B16-F10 melanoma. Results : The results were obtained as follows : 1. In the experiment groups treated with ELP(Ecliptae Herba) Herbal acupuncture, the spleen cell proliferation in BALB/c mouse was significantly increased compared with control group. 2. In the experiment groups treated with ELP(Ecliptae Herba) Herbal acupuncture, the percentage of $CD25^+/CD4^+$, $CD8^+/CD3e^+$, $CD69^+/B220^+$, $NK1.1^+/CD3e^+$cells in C57BL/6 mouse PBMCs was increased compared with control group. 3. In the experiment groups treated with ELP(Ecliptae Herba) Herbal acupuncture, the pulmonary colony formation number of C57BL/6 mice implanted intravenously with B16-F10 melanoma was decreased significantly compared with control group. 4. In the experiment groups treated with ELP(Ecliptae Herba) Herbal acupuncture, MST(Median Survival Time) and ILS(Increase of Life Span) of C57BL/6 mice implanted intravenously with B16-F10 melanoma were increased significantly compared with control group. Conclusions : ELP Herbal acupuncture injection into Chung-wan(CV12) of C57BL/6 mice is considered to be effective in anti-cancer, anti-metastasis and immune response improvement.

• 대한화장품학회지
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• 제30권4호
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• pp.485-489
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• 2004

피부에서 melanin은 자외선 차단의 주요한 역할을 한다. Tyrosinase는 멜라닌 생합성과정에서 초기 단계에 관여하는 중요한 효소로서 이것의 조절을 통한 피부 멜라닌화 억제에 관해 많은 연구가 되어져왔다. 본 연구에서는 해녀콩 추출물에서 mushroom tyrosinase 활성억제, B16F10 melanoma 세포를 이용한 dopa oxidase 활성억제 및 멜라닌 합성 억제 효과를 확인하였다. Tyrosinase mRNA 발현에서의 억제 효과를 확인하기 위하여 RT-PCR을 이용하였으며, $CHCI_3$ 층에서 분리해낸 A 분획에서 tyrosinase mRNA 발현을 억제시킴을 확인하였다.

• 대한본초학회지
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• 제32권2호
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• pp.57-64
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• 2017

Objectives : Yam bean (Pachyrhizus erosus) possess various nutrients, it has been widely used as traditional cosmetic material in Indonesia. The aim of this study was to investigate the anti-oxidant activity and the anti-melanogenic effect of Yambean (Pachyrhizus erosus) extract and its fractions. Methods : The anti-oxidant activity of yam bean extract assessed based on total polyphenol, flavonoid contents, DPPH and ABTS radical scavenging assay. To evaluate anti-melanogenic effects and cytotoxicity of Yambean extract and its fractions, B16F10 melanoma cell was used. Results : In results, total polyphenol content of yam bean water extract (YW) and Yambean 70% ethanol extract (YE) were $1.18{\pm}0.03mg/g$ (mg of gallic acid/g of sample), $1.16{\pm}0.01mg/g$. Total flavonoid contents of YW, YE were $3.55{\pm}0.06mg/g$ (mg of naringin/g of sample), $1.78{\pm}0.03mg/g$. Moreover, YE scavenged DPPH and ABTS effectively in $4mg/m{\ell}$ compared to YW. Cytotoxicity of YE and its fractions in B16F10 melanoma cell was measured using MTT assays. It had no cytotoxicity up to $500{\mu}g/m{\ell}$. Melanin accumulation in B16F10 melanoma cell was induced using alpha-melanocyte stimulating hormone (${\alpha}-MSH$) and 3-isobutyl-1-methylxanthine (IBMX). B16F10 melanoma cell treated with $10-500{\mu}g/m{\ell}$ YE and hexane, ethyl acetate, butanol, $H_2O$ fractions for 24h. Non treated B16F10 melanoma cell (Control) markedly increased melanin contents. In contrast, YE ethylacetate fraction effectively suppressed melanin accumulation in a dose-dependent manner. Conclusion : In conclusion, these results suggest that Yambean extract has the potential as a cosmetic material which possess anti-oxidant and anti-melanogenic activities.

• Journal of Acupuncture Research
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• 제35권4호
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• pp.182-186
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• 2018

Background: The purpose of this study was to investigate the effects of wild ginseng pharmacopuncture on melanin production in B16/F10 murine melanoma cells. Methods: To determine the effect of wild ginseng pharmacopuncture solution on B16/F10 cells, cytotoxicity was evaluated using the 3-(4,5-dimethylthiazol-2-yl)- 2,5-diphenyl-tetrazolium bromide (MTT) method. To observe B16/F10 cell growth, death, and morphological changes, Trypan blue solution was used. The Hosoi method was used to investigate the effect of wild ginseng pharmacopuncture solution on melanin production. The Martinez-Esparza method was used to investigate the effect of wild ginseng pharmacopuncture solution on tyrosinase activity. To determine the pathway involved in the melanogenesis in cells exposed to wild ginseng pharmacopuncture solution, a cell-free tyrosinase was used. Results: Following treatment with $200{\mu}L$ of wild ginseng solution, the cell survival rate was $76.32{\pm}2.45%$ which significantly decreased with higher concentrations (${\mu}L$) of wild ginseng (up to $200{\mu}L$). When $100{\mu}L$ of wild ginseng was used, the cell survival rate was $89.95{\pm}2.07%$. No morphological changes or abnormalities were observed in the B16/F10 murine melanoma cells as observed in the Trypan blue test. Melanin production was significantly reduced to $72.17{\pm}3.74%$ at $100{\mu}L$. Using $100{\mu}L$ of wild ginseng solution, tyrosinase activity was significantly decreased to $80.15{\pm}1.05%$. Wild ginseng pharmacopuncture solution reduced melanin production both directly and indirectly. Conclusion: This study suggests that wild ginseng pharmacopuncture solution may be effective in inhibiting melanin production. Further studies are needed to determine safe and effective clinical applications.

• 생명과학회지
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• 제34권2호
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• pp.105-112
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• 2024

본 연구에서는 차가버섯의 미백 활성 검증을 통하여 화장품 소재로써의 활용 가능성을 확인하였다. 차가버섯 추출물의 항산화 효과를 알아보기 위해 전자공여능을 측정한 결과 농도가 증가할수록 활성도도 증가하였으며 1,000 ㎍/ml 농도에서 82.1%의 우수한 전자공여능을 나타내었다. 또한 미백 효과를 검증하기 위해 tyrosinase 저해활성을 측정한 결과 농도 의존적으로 저해활성이 증가함을 확인하였다. MTT assay를 통해 멜라노마 세포(B16F10)의 세포 생존율을 확인한 결과 100 ㎍/ml 이하 농도에서 80% 이상의 생존율을 나타내었다. 따라서 세포 관련 실험은 25, 50, 100 ㎍/ml 농도에서 진행하였다. B16F10 cell에 차가버섯 추출물을 처리하여 멜라닌 합성과 관련된 단백질 발현을 측정한 결과 농도 의존성에 따라 모든 인자에서 단백질 발현이 억제됨을 확인할 수 있으며 100 ㎍/ml의 농도에서 TRP-1, MITF는 40.1%, 64.2%의 발현량을 나타내었고 tyrosinase 및 TRP-2는 arbutin 보다 단백질 발현 저해가 우수한 것을 확인할 수 있었다. 또한, 멜라닌 합성 관련 mRNA 발현 측정을 위해 B16F10 세포에 차가버섯 추출물을 처리한 결과, 농도가 증가할수록 mRNA 발현이 억제되는 것을 검증하였다. 이에 따라 차가버섯 추출물이 미백 활성을 가지는 화장품 소재로써의 가능성을 확인할 수 있었다.

• Korean Journal of Acupuncture
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• 제29권1호
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• pp.117-130
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• 2012

Objectives : The purpose of this study was to investigate the melanogenesis inhibition effect of Saururus chinensis BAILL (SC) on in B16F10 melanoma cells. Methods : SC was fractionated ethanol extract by the hexane, ethyl acetate, butanol and water. We confirmed the inhibitory effect of tyrosinase activity and melanogenesis of all fraction samples. Results : Hexane fraction of Saururus chinensis BAILL (HSC), ethyl acetate of SC (ESC), and butanol of SC (BSC) were discovered to inhibit tysoinase activity and melanogenesis in the absence or presence of ${\alpha}$-MSH. However, water fraction of SC (WSC) did not affect tyrosinase activity and melanogenesis. In addition, all fractions did not inhibit the catalytic activity of cell-free tyrosinase from B16F10 melanoma cell lines. Conclusions : These results suggest that HSC, ESC and BSC reduce pigmentation by indirectly regulating tyrosinase.

• 공업화학
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• 제31권6호
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• pp.653-659
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• 2020

본 연구에서는 메톡시폴리에틸렌글리콜(methoxy polyethylene glycol)과 올레아놀산(Oleanolic acid) 유도체(mPEG-OA derivative)를 합성하였으며, 합성된 유도체에 대하여 수용액에서의 용해도와 멜라닌 생성억제 효과를 평가하였다. mPEG-OA 유도체의 합성된 구조는 1H NMR, 13C NMR 및 FT-IR로 확인하였다. 수용액에서 mPEG-OA 유도체와 OA의 용해도를 측정한 결과, mPEG-OA 유도체는 13 mg/mL, OA는 0.013 mg/mL로서, mPEG-OA 유도체의 수용성이 OA보다 1,000배 높게 나타냈다. 세포생존율은 B16F10 melanoma cells에서, mPEG-OA 유도체의 세포생존율(250 μM)이 OA로 처리한 세포생존율(62.5 μM)과 비교하여 4배 증가하였다. 멜라닌 생합성 억제 효과는 세포생존율이 영향을 받지 않는 농도에서 측정하였으며, mPEG-OA 유도체는 50 μM의 농도에서 36%, OA는 10 μM의 농도에서 35%의 억제 효과를 나타내었다. B16F10 melanoma cells에서 MITF (microphthalmia-associated transcription factor)의 발현 억제 수준은 mPEG-OA 유도체는 50 μM의 농도에서 59%, OA는 10 uM의 농도에서 49%의 억제 효과를 나타내었다. 종합적으로 mPEG-OA 유도체와 OA의 수용성 및 미백활성을 비교한 결과, mPEG-OA 유도체는 OA보다 뛰어난 수용성을 가지며, 멜라닌 생합성을 억제하는 효과를 나타냄으로써 미백 기능성 화장품 소재로서 응용 가능성이 있음을 시사한다.