• 한국식품영양과학회지
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• 제23권4호
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• pp.604-613
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• 1994

Hydrophilic brown pigments(HBPs) produced during fermentation and aging the domestic Meju and Doenjang were separated by dialysis and chromatography , and their antioxidant activity were measured . The chemical properties of HBP were determined by UV and IR spectrophotometry. HBPs contents were found to be 93.1 mg/g and 183.2mg/g in Meju fermentated for 30 days and 80 days , respectively. The ratio of dialysate to diffusate of the HBPs were appeared to be 70 : 30 and 87 :13 in the Meju fermented for 30 days and 80 days, respectively. and the rtio in the Doenjang aged for 60 days was 91 :9 , indicating that dialysate slowly days, respectively, and the ratio in the Doenjang aged for 60 days was 91 : 9, incidatin that dialysate slowly increased by the fermentation . Both portion exhibited strong antioxidant activity, but more stronger antioxidant activity was found in the dialysate. DEAE-celluose column chromatography showed that dialysate contained more materials eluted by 0.01-0.03M HCI solution than 0.01M acetate buffer, but diffusate showed opposite results. The degree of browning reaction and antioxdiant activity found in the fraction eluted by HCI solution was stronger than that of the fraction eluted by acetate buffer. The fraction eluted from DEAE-cellulose column chormatogrphy was further fractionaged by TLC and found that strong antioxidant activity was present in the fractions which did not possess fluorescenece and showed a negative ninhydrin reaction. TLC fractions of HCI eluant in Meju exhibited a strong absorbance at 260-280nm, but most of other fractions did not show any absorbance at UV region. TLC fractions from dialysate and diffusate showed fairly identical IR spectrum with absorbance at 3400cm-1, 2800cm-3000cm -1, 1600cm, -1 1400cm-1 and 1100 cm-1 , however, in addition to these absorbances, the spectrum from HCI eluant of Meju exhibited a strong absorbance at 1750cm , indicating the carbonic acid or carbonate ester.

• 한국식품영양학회지
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• 제20권4호
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• pp.378-383
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• 2007

To study the characteristics and processing of Kochujang which is rapidly fermented by commercial enzymes, three kinds of Kochujang(KP-FA, KP-FN, and KP-BN) using commercial proteases and one Kochujang(KM) using Meju were prepared and their qualities investigated. There were only small differences in pH and acidity between each Kochujang. The moisture contents were high tendency in the three kinds of Kochujangs using the commercial proteases at 20 days of fermentation. Reducing sugars had a tendency to decrease during the fermentation in the Kochujangs using the proteases. During the first half of fermentation, the Kochujangs made with proteases showed higher amino nitrogen contents than the Kochujang(KM) made using Meju. Acidic protease activity was high in KP-FA at 20 days of fermentation and neutral protease activity was high in KP-FN and KP-BN at the beginning of fermentation. The Kochujangs made using the proteases, through 20 days of fermentation, obtained high preference in the sensory evaluation for color, texture, and overall acceptability. However, the hot taste was stronger in these Kochujangs during the fermentation.

• Journal of Microbiology and Biotechnology
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• 제27권2호
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• pp.251-261
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• 2017

Initially, we screened 18 Aspergillus sojae-like strains from Aspergillus spp. isolated from meju (Korean traditional fermented soybean brick) according to their morphological characteristics. Because members of Aspergillus section Flavi are often incorrectly identified because of their phylogenetic similarity, we re-identified these strains at the morphological and molecular genetic levels. Fourteen strains were finally identified as A. sojae. The isolates produced protease and ${\alpha}-amylase$ with ranges of 2.66-10.64 and 21.53-106.73 unit/g-initial dry substrate (U/g-IDS), respectively, which were equivalent to those of the koji (starter mold) strains employed to produce Japanese soy sauce. Among the isolates and Japanese koji strains, strains SMF 127 and SMF 131 had the highest leucine aminopeptidase (LAP) activities at 6.00 and 6.06 U/g-IDS, respectively. LAP plays an important role in flavor development because of the production of low-molecular-weight peptides that affect the taste and decrease bitterness. SMF 127 and SMF 131 appeared to be non-aflatoxigenic because of a termination point mutation in aflR and the lack of the polyketide synthase gene found in other A. sojae strains. In addition, SMF 127 and SMF 131 were not cyclopiazonic acid (CPA) producers because of the deletion of maoA, dmaT, and pks/nrps, which are involved in CPA biosynthesis. Therefore, A. sojae strains such as SMF 127 and SMF 131, which have high protease and LAP activities and are free of safety issues, can be considered good starters for soybean fermentations, such as in the production of the Korean fermented soybean products meju, doenjang, and ganjang.

• 한국식품영양학회지
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• 제31권5호
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• pp.712-719
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• 2018

This study was carried out to obtain basic data on Korean traditional Meju collected from 18 regions (TM1~TM18) in Korea and to define and control quality. The shape of Meju was mostly rectangular and the weight was 0.84~2.04 kg. The physicochemical analysis showed: pH, 5.31~8.21; total acidity, 0.91~2.74%; moisture content, 4.79~42.16%; and soluble protein content, 41.37~23.48%. Hunter color values for L (lightness), a (redness), and b (yellowness) ranged from 39.07~67.92, 3.57~8.87, and 7.48~20.67, respectively. The amino nitrogen contents of all samples were in the range of 257.29 to 839.58 mg% and TM13 showed the highest content (839.58 mg%). Total viable cells, yeast and mold counts of Meju were 8.43~5.91 log CFU/g, 2.48~5.19 log CFU/g, and 3.42~7.48 log CFU/g, respectively. Based on the results, it is proposed that quality standards and management of Meju fermentation conditions and information about different varieties of soybeans used should be made available.

• Applied Biological Chemistry
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• 제41권6호
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• pp.405-409
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• 1998

본 연구는 재래간장의 원료 대두로부터 자숙과 메주제조 과정중의 당류의 행동을 추적하고 가당에 의한 젖산발효와 알콜발효의 효과를 조사하기 위하여 실시되었다. G8메주와 SB메주 제조과정 중 수용성 당류함량이 대폭 감소하였다. G8메주와 SB메주로 만든 저염간장 소화액에 젖산균 Pediococcus halophilus와 효모 Zygosaccharomyces rouxii 및 Candida versatilis의 종모배양액을 접종한 결과, 5% glucose를 첨가한 G8간장과 SB간장에서는 모두 급속히 젖산발효와 알콜발효가 일어났으나 당 무첨가 간장에서는 발효가 일어나지 않았다. 종모배양액 접종 후 160시간 후 가당 G8간장과 가당 SB간장에서 각각 0.46%, 0.88%의 젖산과 1% 및 약 2%의 알콜을 함유하고 있었다.

• 한국식품영양학회지
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• 제12권5호
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• pp.490-495
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• 1999

A thermotolerant yeast Saccharomyces cerevisiae OE-16 was isolated from traditional Meju was investigated on their physiological characteristics and ethanol fermentation ability. Saccharomyces cerevisiae OE-16 were able to grow up to 45$^{\circ}C$ and 40% of glucose. Saccharomyces cerevisiae OE-16 was also resistant to 15% of KCl 1,200ppm of Pb2+, Hg2+ and 500ppm of potassium sorbate. From 20% glucose media Saccharomyces cerevisiae OE-16 produced 83.4g per liter of ethanol at 3$0^{\circ}C$ and 9.5g per liter of ethanol at 4$0^{\circ}C$ for 72 hours.

• 한국식품과학회지
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• 제29권6호
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• pp.1158-1165
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• 1997

본 연구에서는 고추장의 품질향상과 전통고추장과 유사한 관능적 요인을 갖는 고추장을 제조하기 위해 찹쌀고추장으로 유명한 전라북도 순창지방의 고추장을 모델로 설정하여 순창 현지의 미생물, 기후 등의 자연환경을 반영한 메주를 0, 20, 40, 60일 발효한 후 고추장을 제조하여 발효기간을 달리한 메주가 고추장 숙성 중 미생물과 효소력에 미치는 영향을 조사하였다. 세균은 모든 시험구에서 숙성 30일에 최대균수를 보였으며, 곰팡이는 0, 20일 발효 메주로 제조한 고추장에서는 지속적으로 감소하다가 숙성 60일 경에는 발견되지 않았으며, 40, 60일 발효 메주를 이용하여 제조한 고추장은 숙성 초기 $10^5$의 생균수를 나타냈으나 숙성 60일 까지 급격히 감소한 후 $10^2$의 일정한 수준을 유지하였다. 한편 효모는 0, 20일 발효 메주고추장에서는 숙성 전기간 통안 발견되지 않았으며, 40, 60일 발효 메주고추장에서는 숙성 15일부터 발견되기 시작한 후 계속 감소하였다. 0, 20일 발효 메주를 이용한 고추장의 경우 모든 amylase역가가 숙성초기부터 계속적으로 감소하였으나 40, 60일 발효 메주를 이용한 고추장에서는 ${\alpha}-amylase$의 경우 숙성 15일에 각각 115.83, 113.85 unit/g 으로 최대 역가를 보였으며, ${\beta}-amylase$는 숙성 30일경에 각각 7.37, 6.57 unit/g으로 최대 역가를 보였다. glucoamylase는 40일 발효 메주고추장의 경우 숙성 초기 4.78 unit/g에서 숙성 45일의 2.23 unit/g까지 53.33%가량 감소하다가 다시 증가하여 숙성 90일경 에는 2.49 unit/g의 역가를 보였다. 산성 protease는 숙성 30일경에 모든 고추장에서 최대값을 보였으나, 중성 protease는 숙성 15일 경에 최대값을 보였다. 즉 모든 amylase, protease의 역가는 40일 발효 메주고추장에서 가장 높은 값을 나타냈다.

• 한국식품과학회지
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• 제29권6호
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• pp.1166-1174
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• 1997

본 연구에서는 고추장의 품질향상과 전통 고추장과 유사한 관능적 요인을 갖는 고추장을 제조하기 위해 고추장으로 유명한 전라북도 순창지방의 고추장을 모델로 설정하여 순창 현지의 미생물, 기후 등의 자연환경을 반영한 메주를 0, 20, 40, 60일 발효시켜 고추장을 제조하여 발효기간을 달리한 메주가 고추장 숙성 중 품질 특성에 미치는 영향을 조사하였다. 40일 발효 메주 고추장의 경우 숙성 90일 경과 후 총유기산의 함량이 다른 시험구에 비해 $1.14{\sim}2.54$배 높은 함량인 584.23 mg%를 나타났으며, 특히 lactic acid, succinic acid, citric acid의 함량이 다른 시험구에 비해 높았고, 바람직하지 못한 역할을 하는 것으로 판단되는 pyroglutamic acid의 함량은 적었다. 고추장 숙성기간 중 유리당은 fructose, glucose, sucrose, maltose가 검출되었으며 숙성이 진행되면서 이당류인 maltose와 sucrose는 감소하여 검출되지 않았으나 glucose와 fructose는 전체 유리당에 $82.27{\sim}100%$를 차지하는 것을 볼 때 고추장의 주요한 유리당으로 생각된다. 유리아미노산은 Asp를 포함한 17종이 검출되었으며 이 중 Glu의 경우 0, 20일 발효 메주 고추장과 40, 60일 발효 메주 고추장간에 큰 함량차이를 보여 Glu가 고추장에 있어 구수한 맛의 주체 일 것으로 사료된다. 관능검사결과 고추장 품질에 바람직한 특성으로 생각되는 단맛, 구수한 맛, 알코올냄새 등은 숙성기간이 증가하면서 모두 증가하는 것으로 나타났으며, 바람직하지 못한 특성인 쓴맛은 감소하였고 매운맛과 퀘퀘한 냄새는 불규칙한 증감현상을 보였다. 분산분석 실시 결과 고추장 숙성 30일부터 시료간의 유의성을 인정할 수 있는 것으로 나타났으며, 특히 맛보다는 냄새의 유의성이 높은 것으로 나타났다. 또한 기호도 측면에서 볼 때 모든 시험구에서 숙성이 진행됨에 따라 냄새, 맛 모두 좋아지는 것으로 나타났으며, 특히 40일 발효 메주를 이용한 고추장의 경우 숙성 30일부터는 다른 고추장에 비해 냄새, 맛 모두 우수한 것으로 나타났다.

• 한국식품영양과학회지
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• 제26권3호
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• pp.383-389
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• 1997

Doenjang(fermented soybean paste) was prepared by the series of processes including soaking, cooking, first fermentation(3 days at 30$\pm$2$^{\circ}C$) for the preparation of meju(soybean koji) after inoclulation of Aspergillus oryzae, and further fermentation (60 days at 30$\pm$2$^{\circ}C$) for the ripening after addition of salt 13% to meju. the crude phenolics extracted from defatted soybean and doenjang were fractionated onto the neutral phenolics(isofavonoids) and acidic phenolics(phenolic acids), respectively. Composition and antioxidative characteristics of phenolic fractions were determined. The neutral phenolic fractions contained genistin, genistein and daidzein; on the other hand, acidic phenolic fractions had syringic acid and seven other components. The content of genistin in doenjang dramatically decreased at the early stage of fermentation, whereas the content of genistein rather increased. In addition, the content of syringic acid of acidic phenolic fractions were increased during fermentation. These changes in individual phenolic components affected the antioxidative activity of neutral phenolics or acidic phenolics. antioxidative activity of phenolic compounds were evaluated during soybean fermentation. The antioxidative and free radical scavenging activity of neutral phenolic fractions and acidic phenolic fractions on linoleic acid autoxidation were also investigated.

• 한국식생활문화학회지
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• 제28권6호
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• pp.640-650
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• 2013

Soy sauce, a basic ingredient in Asian cuisine, is made of fermented soybeans, salt, water, and barley or wheat flour. The sensory characteristics of soy sauce are not only determined through its main ingredients but also by various flavor compounds produced during the fermentation process. This study was conducted to identify the sensory attributes of five different commercial soy sauce samples that differ in Meju types (traditional Meju or modified Meju) and usage types. Thirty three sensory attributes, including appearance (1), odor (16), flavor (14), and mouthfeel (2) attributes, were generated and evaluated by eight trained panelists. The collected data were analyzed by analysis of variance and principal component analysis. There were significant differences in the intensities of all sensory attributes among the soy sauce samples. Soy sauce made with traditional Meju had high intensity of fermented fish, beany, musty odors and salt, Cheonggukjang, fermented fish flavors. Whereas two soy sauces made with modified Meju were strong in alcohol, sweet, Doenjang, roasted soybean flavors. Two soy sauces for soup made with modified Meju had medium levels of briny, sulfury, fermented odors and bitter, chemical flavors and biting mouthfeel characteristic.