• Journal of Forest and Environmental Science
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• v.34 no.1
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• pp.61-65
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• 2018

To know if small changes in rearing water temperature and density affect the number of bacterial and fungal colonies in metamorphosed frogs, Dybowski's frog tadpoles were reared from Gosner 25-26 stages at either low ($1^{\circ}C$ low to ambient water temperature), ambient, or high ($1^{\circ}C$ high) water temperature (each 15 tadpoles in 20 L water) condition and at either low (10 tadpoles/20 L water), medium (20 tadpoles), or high (30 tadpoles) density condition. Immediately after metamorphosis, we sampled bacteria and fungi from skin, liver, and heart of six metamorphosed frogs, randomly selected for each treatment group. After separate incubation of bacteria and fungi on 3M Petrifilm plates, we counted the number of bacterial and fungal colonies appeared on the plates and compared the numbers among the temperature and density treatment groups. For temperature treatment, high-temperature group had fewer bacterial colonies, while low-temperature group had more fungal colonies than the other two groups. For density treatment, low-density group had fewer bacterial colonies than the other two groups, but the number of fungal colonies were not different among the groups. Our results suggest that small increased rearing water temperature and lowered rearing density could potentially reduce pathogens in farming frogs.

• Korean Journal of Oriental Medicine
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• v.12 no.2 s.17
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• pp.47-62
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• 2006

This research is intended to analyze the necessity of the integrated organization for the performance in order to the extension of scale and the systematic promotion concerning to the Korean medicine informational project, and to research the promotional method of Korean Medicine information through the constitution of organization for preparing the informational basic. There are three main important subjects to be done in this research project. The initial issue is to devise a comprehensive and systematic plan of Korean medicine information according to the Korean Medicine Rearing Raw('03. 8). Furthermore, it is necessary to reinforce the integrated informational ability of Organizations concerning to the Korean Medicine. Finally, with the above works accomplished, the overall standard of Korean Medicine Information should be settled. Initially, we compose an advisory committee of the Korean Medicine Information in the first year. The members of the committee include the informational coordinates of related government agencies, the experts in Korean Medicine Information, and specialists in the domains of computer science and information. In the next phase, we derive the promotional strategy for 'Center for Korean Medicine Information'. In other words, by means of analyzing the current issues and outstanding problems of on-going research projects and environments, the ultimate directions of the whole activities are established. Also, we build the medium and long-range road map of Korean Medicine Information Research by setting strategy for the policy projects related to the area. With the various research activities in this project, we can achieve the following practical strategic benefits. Firstly, we can lay a foundation for the Korean Medicine Information by establishing the fundamental plan for 'Korean Medicine Information Center'. Secondly, information strategic projects about 'Korean Medicine Information Center'. Secondly information strategic projects about 'Korean Medicine Information Development' can be performed in more systematic fashion and promoted by stages. Finally, by confirming the contents of the Korean Medicine of KMS system, we invent an opportunity of new project in the field of the Korean Medicine Information by means of practicing and managing the 'korean Medicine Information Custom Service'.

• Journal of the Korean Society of Safety
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• v.33 no.1
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• pp.148-161
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• 2018

A traffic fatality by young people marked average annual decrease of 4.5% since 2011. Meanwhile, a traffic fatality by senior over 65 years old marked average annual increase of 7.9% for the last five years which means that the annual increase of traffic fatality by senior will be a serious problem. This study started questioning that senior drivers over 65 years old did not retain the same causal factor of fatal traffic accidents and thus extensively analyzed a risk of it by age group quantitatively, dividing the senior driver group into the early, middle and latter stages. Depending on the aging level, the risk of traffic fatality showed a wide difference in seven different types of traffic accidents generally, and happened to increase with latter and middle parts of the senior driver more than the early part. Therefore, this study proposes four policy suggestions: 1) The senior driver need to be offered customized driving educations and the improvement of road environment is also recommended. 2) Political assistance is needed to support and guide a safety related technology installation for the new or existing car. 3) Renewal of driving license and an aptitude test(physical examination, cognitive test) for drivers over 75 years old should take in a less than 3 years and an additional road test is needed as occasion demands. 4) Like the United States and Europe, development and extension of customized treatment guidebook for medical teams who examine senior drivers is needed and establishment of education and administration system that a supervisor of driving license renewal can impose safety restriction and American anonymity reporting system is considered to institutionalize in the medium to longer term.

• Journal of Microbiology and Biotechnology
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• v.15 no.1
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• pp.175-182
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• 2005

Abstract Salmonella pathogenicity island 1 (SPI1) gene expression is regulated by many environmental signals such as oxygen, osmolarity, and pH. Here, we examined changes in the expression level of various regulatory proteins encoded within SPI1 in response to three different concentrations of NaCl, using primer extension analysis. Transcription of all the regulatory genes tested was activated most when Salmonella were grown in Luria Broth (LB) containing 0.17 M NaCl. The expression of hilA, invF, and hilD was decreased in the presence of 0.47 M NaCl or in the absence of NaCl, while hilC expression was almost constant regardless of the NaCl concentration when Salmonella were grown to exponential phase under low-oxygen condition. The reduced expression of hilA, invF, and hilD resulted in lower invasion of hilC mutant to the cultured animal cells when the mutant was grown in the presence of 0.47 M NaCl or in the absence of NaCl prior to infection. Among the proteins secreted via the SPI1-type III secretion system (TTSS), the level of sopE2 expression was not influenced by medium osmolarity. Various effects of osmolarity on virulence gene regulation observed in this study is one example of multiple regulatory pathways used by Salmonella to cause infection.

• Proceedings of the Mineralogical Society of Korea Conference
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• 2002.05a
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• pp.1-3
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• 2002

The eastward extension of the suture zone between the Sino-Korea and Yangtze cratons in the Korean Peninsula and Japanese islands remains debatable (Hiroi, 1981; Cluzel et al., 1991; Yin and Nie, 1993; Sohma and Kunugiza, 1993; Isozaki, 1997; Arakawa et at., 2000), and is related to our understanding of the continent-continent collision orogeny. The collision orogeny varies in tectono-metamorphic processes and the timing differs from place to place, as exemplified by the absence of coesite and micro-diamond in the Korean Peninsula and Japanese islands, because it is a long-lived process of more than several tens of million years from subduction to exhumation in the Wilson cycle, and because the suture zone extends more than several thousand kilometers with a curved shape from the Qinling area of China to the Hida highland area of Japan. Hiroi (1981) is the first paper to correlate the Unazuki metamorphic rocks of the Hida metamorphic belt in Japan with the Ogcheon belt in the Korean Peninsula based on the presence of 240 Ma medium P/T metamorphic rocks in both belts, but there is a lack of recent studies on this correlation. To resolve the correlationship, there are two approaches: 1) petrological studies characterizing the origin and P-T history of rocks and 2) in-situ micro-dating of fine-grained, zoned minerals of zircon, monazite, uraninite and thorite using the EPMA (U-Th-Pb chemical dating or CHIME depending on calibration method) and the SHRIMP (Sensitive High-resolution ion Microprobe) to decipher the timing of geological events. As a first step of these approaches, micro-dating was undertaken to rocks of the Hida metamorphic belt and its Mesozoic cover (Tetori Group) in the Hida highland area, central Japan.

• Archives of design research
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• v.14 no.2
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• pp.155-162
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• 2001

Recently, as the increase of internet users and the needs of the new educational paradigm, many educational institutions go into the cyberspace system. Especially, it is increase that the cooperative extension for supplying the degree and the remote lectures in the university itself. Mostly those lectures have the form of discussion with theoretical course in the center. It is needed to the development of educational tools for the other courses. The cyber education systems are due to be developed more and more. So we should consider the effective usage of recent techniques like this. Accordingly, I'd like to say what is the method of using effectively the cyber communication. It would be considered about the part of cyber communications and the educational effects that has classified into three parts: the cyber communication as a educational medium, the communication for the cooperative studies, and the theological background of the cyberspace communication. Moreover it is proposed the two parts of the effective study directions; the constitution of the community study, and the effective study factors.

• Korean Journal of Soil Science and Fertilizer
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• v.34 no.5
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• pp.333-341
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• 2001

The Compost-decomposing-bacteria was isolated from livestock compost containing sawdust. The isolated bacteria was identified as Bacillus subtilis LYH201 by the method of the composition of the fatty acid with MIDI system and Bergey's manual. Cloning of CMCase encoding gene was accompanied by shotgun method. The pLK100 have yellow activity ring on CMC medium, that was carried 2.2 kb insert DNA in pBluescript II $SK^+$ vector, named BglC gene. The BglC was very similar to Pectobacterium carotovorum Gun_CLOAB(P15704) with score of 57% identity and 71% homology over 508 aa. The BglC was measured molecular weight 56 kDa by CMC-SDS-PAGE. Optimum cellulase activity Bacillus subtilis LYH201 was temperature $50^{\circ}C$ and pH 7.

• The Korean Journal of Mycology
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• v.39 no.2
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• pp.148-150
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• 2011

Cordyceps militaris(CM) has been used as a traditional folk medicine to strengthen the immune system and regain energy for hundreds of years in Far East Asian countries. In the present studies, we determined that the stimulatory effect of Cordyceps militaris on testosterone production in male mouse rats. Results illustrated that changes of the body weight, food and water intake of the rats were not observed in this study but the concentration of testosterone in the serum of the rats was significantly increased by CM(p < 0.05). Therefore fruiting bodies of CM grown on the drone bee medium may be an integrative medicine for the treatment of reproductive problems caused by insufficient testosterone levels in human males.

• The Journal of the Korea Contents Association
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• v.5 no.6
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• pp.273-282
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• 2005

The purpose of this study is how to apply to e-Commerce of parts procurement system in the Japanese Automobile Industry and examine the influence of that through the case studies of automobile makers. In the Japanese automobile industry, B2B e-Commerce of parts procurement system have a shifted from closed circuits through VAN to opened IP-VPN and eventually to the JNX of internet environment. The Japanese automobile industry depends mainly on IP-VPN and JNX, especially EDI on a one to one basis with parts procurement suppliers. As to the future direction of B2B e-Commerce of parts procurement in the Japanese automobile industry, many of commodity parts will be traded among established participants on internet websites owned by automobile maker groups. Finally, for improvement of B2B e-Commerce of parts procurement in the Japanese automobile industry I suggested that there are 'revitalize of e-Marketplace', 'improvement of IT and business process', 'extension of B2B e-Commerce among small and medium sized suppliers'.

• Korean Journal of Acupuncture
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• v.31 no.4
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• pp.218-224
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• 2014

Objectives : Zizyphus jujuba fruits(ZJF), a traditional Korean medicine has various biological activities such as anti-inflammatory, anti-oxidative and neuro-protective effects. However, it is still unclear whether ZJF has any biological effect on anti-aging. In this study, we examined the effect of ZJF on lifespan and thermal stress in C. elegans. Methods and Results : ZJF water extracts were fermented for 7 days(F7-ZJF) and 14 days(F14-ZJF) by Laetiporus sulphureus to increase secondary metabolites such as aglycone of flavonoids and terpenoids. In the lifespan assay, ZJF water extracts and fermented ZJF were treated on the agar medium plate with age synchronized egg stage of C. elegans. Treatment of F7-ZJF-$200{\mu}g/mL$ with OP-50 E. coli and F14-ZJF-$200{\mu}g/mL$ with OP-50 E. coli significantly increased life span of C. elegans(N2) at thermal stress condition of $25^{\circ}C$. Moreover mRNA levels of lifespan associated HSP 16.1, HSP 70, and HSF-1 were increased at thermal stress condition of $25^{\circ}C$. However, in the equilibration temperature of $20^{\circ}C$ after stress condition of $35^{\circ}C$ for 2 hr, F-14-ZJF-$200{\mu}g/mL$ treatment decreased the levels of heat shock protein in hsp16.2/GFP C. elegans. Conclusions : Our study indicates that prolong role of fermented-ZJF in C. elegans is mediated by control HSPs production.