• Journal of Bio-Environment Control
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• v.29 no.4
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• pp.421-427
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• 2020

In this study, we investigated the effect of nutrient supply periods on the fruit growth and quality of 'Duke' blueberry. The nutrient solution was contained with NO₃-N 4.6, NH₄-N 3.4, PO₄-P 3.3, K 3.0, Ca 4.6, and Mg 2.2 mmol·L^-1, and EC in the nutrient solution was 1.5 ds.m^-1. In 2017 and 2018, an individual blueberry bush was supplied with 8 L of the nutrient solution per week. In 2018, the drainage water quality of growing medium and fruit quality was investigated. The nutrient supply was started from April 01, and stopped at 15 days intervals as follows, 15 days before final harvest, at the final harvest date (June 30), 15 days after final harvest (DAFH), 30 DAFH, 45 DAFH, and 60 DAFH. The content of inorganic components in the growing medium was not significantly different by the stop time of nutrient supply, but the content of phosphorus (P₂O₅) tended to increase with the delay of stop time of nutrient supply. There were no significant differences in the fruit quality characteristics in terms of size, sugar content, and acidity among the different stop time of nutrient supply. The blueberry yield was tended to decrease with the delay of periods of nutrient supply, while the lowest yield of 1.8 kg was recorded when nutrient supply stopped at 45 and 60 DAFH.

• Korean Journal of Medicinal Crop Science
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• v.13 no.6
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• pp.234-240
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• 2005

This study was conducted to identify cross compatibility with table grape and pollen characteristics by flower type in Vitis amurensis Rupr. (GW-22, GW-45, GW-56, GW-200, GW-202 and GW-300) collected in Gangwon area. Flower structure, germinability and morphology of pollen, and cross compatibility with table grape were examined. Flowers of GW-22 and GW-56 had an aborted gynoecium and five erect fdaments, and all of them was male. The percentage of pollen germination of GW-22 and GW-56 on the agar medium was 27.6-29.8%. Flowers of GW-45, GW-200, GW-202 and GW-300 had well-developed pistils and five stamens with curled filaments. The percentage of pollen germination of female plant on the agar medium was 0%. Pollen grail)s from GW-22 and GW-56 (male flower) have symmetrically distributed three furrows on their surface and each furrow contains a germ pore at the equatorial level (Tricolporated). GW-45, GW-200, GW202, and GW-300 (Female flower) produced pollen grains with no germinative structures (acolporated). In self-pollina-tion of female flowers, berries of clusters were no berry and no seed. Therefore, flower structure in V. amurensis proved to be that of other dioecious Vitis species. In cross combination of female and male flower of V. amurensis made normal seeds. In cross combination of table grape and V. amurensis of male flower made normal seeds. But, in cross combination of female flower of V. amurensis did not make seeds. Therefore, V. amurensis was cross compatible with vitis spp, and V. amurensis varieties may contribute to breeding studies of Vitis spp.

• Journal of Mushroom
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• v.7 no.1
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• pp.27-36
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• 2009

Pleurotus ferulae is an edible mushroom found on a medicinal plant, Ferula assa-foetida, in centeral China. This study was carried out to investigate the cultural characteristics of P. ferulae strains. Characteristics of mycelial growth were investigated for 5 strains of P. ferulae. All the 5 strains showed the best mycelial growth at $25^{\circ}C$ and their growth rate was very low at $35^{\circ}C$. The colony diameter reached to 45~72 mm after 7 days at $25^{\circ}C$. Malt extract agar medium was the best for mycelial growth of the species both in hyphal length and density. Mycelial growth was not affected by various pH from 6.0 to 8.0. An optimal carbon source was arabinose and an optimal nitrogen one was arginine. And an effective substrate for the mycelial growth was 8 to 2 mixture of oak sawdust and cottonseed meal.

• Journal of Mushroom
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• v.7 no.4
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• pp.182-186
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• 2009

Single ascospores was isolated in Yesan-gun, Chungnam in 2006 with Cordyceps militaris. This study was carried out to select the superior isolates which produce more fruiting body and contain more cordycepin contents. Five single ascospore isolates were cultured on brown rice pupae medium and they formed fruiting bodies. To select better isolates, each isolates was hybridized and then the characteristics and cordycepin contents were investigated. After biological efficiency and codycepin contents were examined, finally C0601S05 was selected. Top-cross test of isolate C0601S05 was carried with 29 single ascospores. From this test, isolate C0601S05${\times}$C0601S33 and isolate C0601S05${\times}$C0601S43 containing highest cordycepin contents were selected. Codycepin contents of C0601S05 was 246mg% and those of C0601S05${\times}$C0601S33 and C0601S05${\times}$C0601S43 were 325mg% and 280mg%, respectively. C0601S05${\times}$C0601S33 was registered as Yedang 3 at the Korea seed & variety service.

• Research in Plant Disease
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• v.16 no.1
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• pp.97-100
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• 2010

Angular leaf spot of strawberry caused by Xanthomonas fragariae occurred sporadically in the farmers open field at Sugok-myon, Jinju city, and greenhouse at Okjong-myon, Hadong-gun, Gyeongnam province, Korea. Typical symptoms of angular leaf spot initially appeared as a minute, water-soaked, rotted lesion on the lower leaf surface and the minute spots expanded to form angular leaf spots, usually delimited by small veins. The lesion was translucent under the transmitted light and dark green under reflected light. The slow growing pathogen was a gram-negative, motile bacterium with single polar flagellum, and producing yellowish orange pigment on YDC medium. It showed very strong pathogenicity on the leaves of strawberry. On the basis of biological characteristics and pathogenicity test on host plants, the bacteria was identified as a Xanthomonas fragariae. This is the first report on angular leaf spot of strawberry caused by X. fragariae in Korea.

• Journal of Embryo Transfer
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• v.22 no.4
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• pp.251-255
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• 2007

This study was carried out to examine on developmental competence of Hanwoo embryos cultured in vitro according to culture conditions and freezing methods. The in vitro developmental competence to blastocyst stage at Day 8 of culture in SOF was significantly (p<0.05) higher than that in CR1aa (30.3% vs. 18.4%). The in vitro developmental rate of morula and blastocysts cultured in group culture was significantly (p<0.05) higher than that in individual culture (41.4% and 36.0% vs. 21.1% and 10.5%, respectively). The cell number of Day 8 blastocysts in group culture was significantly (p<0.05) higher than that in the individual culture ($120.1{\pm}12.8\;vs.\;94.1{\pm}12.1$, respectively). The survival rates of frozen-thawed balstocysts that were exposed in 1.5 M ethylene glycol or 1.5 M ethylene glycol containing 0.1 M sucrose were 77.5% and 78.7%, respectively. The survival rates of blastocysts cultured for 48 h in slow freezing and vitrification was not significantly different (73.3 and 74.0%). In conclusion, in vitro developmental competence of bovine embryos was influenced on the culture medium (SOF) and culture method (Group culture). Survival rate of frozen-thawed of bovine embryos was not influenced on freezing solutions and freezing methods.

• Journal of Pharmacopuncture
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• v.23 no.4
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• pp.212-219
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• 2020

Objectives: Nowadays cancer treatment is an important challenge in the medical world that needs better therapies. Many active secretions produced by insects such as honey bees used to discover new anticancer drugs. Bee venom (BV) has a potent anti inflammatory, anti cancer and tumor effects. The aim of present study is evaluation of anticancer effects induced by Apis mellifera venom (AmV) on cell Lines. Methods: AmV was selected for study on cancer cell lines. Total protein, molecular weight and LD50 of crude venom were determined. Then, cells were grown in Dulbecco's Modified Eagle medium supplemented with 10% fetal bovine serum and 1% antibiotics. The A549, HeLa and MDA-MB-231 cell Lines were exposed by different concentration of AmV. The morphology of cells was determined and cell viability was studed by MTT assay. Evaluation of cell death was determined by and DNA fragmentation. Results: The results from MTT assay showed that 3.125 ㎍/mL of A549, 12.5 for HeLa and 6.25 ㎍/mL of MDA-MB-231 killed 50% of cells (p < 0.05). Morphological analysis and the results from hoescht staining and DNA fragmentation indicated that cell death induced by AmV was significantly apoptosis. Conclusion: The data showed that using lower dosage of AmV during treatment period cause inhibition of proliferation in time and dose dependant manner. Findings indicated that some ingredients of AmV have anticancer effects and with further investigation it can be used in production of anticancer drugs.

• Journal of Mushroom
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• v.18 no.4
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• pp.331-338
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• 2020

This study was conducted to cultivate new Lepista nuda varieties with shorter cultivation period and better fruiting body compared to that of wild strains, for mass production and commercial application. Eighteen genetic resources of L. nuda were collected and grown in boxes using rice straw-fermented growth medium. Four lines with fruiting bodies were formed and selected as cross-breeding lines. Although 657 combinations were crossed through monospore crossing, only 17 combinations were bred between the 'CBMLN-19' line and the 'CBMLN-30' line. Among them, 8 lines with fast mycelial growth and high density were selected. After inoculating the rice straw-fermented growth medium with 14 genetic resources and 8 cross-breeding lines, their incubation period was investigated. Six of the cross-breeding lines completed their incubation in 20 days, while 7 of the 14 genetic resources took more than 40 days to complete their incubation, reducing the incubation period by more than 20 days in most cross-breeding lines. After the incubations were completed, the clay loam soil was covered with for post-cultivation, and when the mycelial cultivation was complete, the formation of fruiting bodies was induced after scraping the mycelial bodies under these environmental conditions: 14℃, 95% relative humidity or higher, and 1,500 to 2,000 ppm CO2 concentration. The temperature was reduced to 6℃ at night, resulting in a low temperature shock. Thus, 4 lines of fruiting bodies occurred from two genetic resources 'CBMLN-31' and 'CBMLN-44' and two cross-bred lines 'CBMLN-96' and 'CBMLN-103'. After inoculation, the longest period for fruiting bodies to occur was 100 days for the control:, the genetic resource 'CBMLN-31', and the shortest period (45 days) was observed for the cross-breeding line 'CBMLN-103'. The result of the investigation of the fruiting body characteristics shows that the cross-bred line 'CBMLN-103' showed a small form with 1.9 g of individual weight and 123validstipes per box, which was the highest incidence among the four lines. Another cross-bred line, 'CBMLN-96', had an individual weight of 5.5 g, which is larger than that of 'CBMLN-103'; however, the number of valid stipes per box was 30 less than that of 'CBMLN-103'. Quantity analysis showed that the control, 'CBMLN-31', had the highest quantity of 783 g per box, followed by the cross-bred line, 'CBMLN-96' with 165 g per box, and then the 'CBMLN-103' with 232 g. The quantity of the two crossbred lines was lower than that of the control 'CBMLN-31'; however, the amount of fruiting bodies was higher, and the cultivation period was shortened by 32 to 33 days. Therefore, these two lines would be selected as superior lines.

• Horticultural Science & Technology
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• v.30 no.1
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• pp.34-41
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• 2012

The study was conducted in a glasshouse to examine the possibility of producing paprika plug seedlings in a newly developed inert phenolic foam growing medium. Plug seedlings of 'Fascinato' paprika were grown in five media, Grodan rockwool (Grodan Co. Ltd., Denmark), UR rockwool (UR Co. Ltd., Korea), phenolic foam LC (Smithers Oasis Korea Co. Ltd., Korea), phenolic foam RC, and phenolic foam 3813-4 all in a pellet type. Seeds were germinated in a growth chamber ($25^{\circ}C$, 90% RH, dark) for 4-5 days and then seedlings were grown in a glasshouse with nutrient solution supplied by an overhead irrigation system. Seedling growths were measured 20 days after sowing. The medium pH was the highest in the Grodan rockwool, and medium EC was the highest in phenolic foam 3813-4, although no nutritional excess disorders were observed. Germination rates of paprika were higher than 90% in all the media. Plant height, stem diameter, T/R ratio, leaf area, and chlorophyll showed a similar to those in the rockwool medium. Number of leaves, length of the longest root and dry weights of shoot were not significantly different among treatments. Overall, phenolic foam LC and RC produced seedlings with a similar growth as the rockwool medium. The results obtained suggest that rockwool can be replaced with a new material such as phenolic foam in the commercial scale production of plug seedlings of 'Fascinato' paprika.

• Journal of Science of Art and Design
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• v.11
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• pp.79-108
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• 2007

"Printing is an Art of reproduction & a Technique of However printing has extended its limitation from a genre of reproduction itself and now it stands on the turning point as an art such as painting or sculpture which expresses artist's originality. Early Printing has had relations in depth with press printing in terms of information recording, preservation, and transmission. It was acknowledged value in a way of information satisfaction different from how it is valued as a pure art today. But, later printing has transferred its function from a mean of reproduction to pure art due to the development of printing skills and photography invention. It can be said that the concept of modern printing is taking over its genealogy as a creative work not as just printing. Also its expression capability is widen to dimensional printing and high-tech multimedia from original tradition techniques. As we discussed above, modern painting is very open to various changes. This modern painting aspect can be seen as an extended interpretation of 'board' concept. This dissertation raises a question why monotype and monoprint couldn't secure its position in printing history in spite of numerous artists' tryouts in its way. Monotype and monoprint fundamentally based on intaglio technique in its history. Yet, its systematic study hasn't been worked out. This is because of the lack of recognition of monotype and monoprint's originality as printing. Especially in monoprint, it has known as an early stage in copperplate printing process which is an attempt to solve the technique limitation or trial work for edition. Likewise the reason why monotype and monoprint remains at the edge of printing border ambiguously is because of conceptual, technical characteristics which are against traditional printing. In traditional printing, the concept of board is important as a method of reproduction. Different from the fact, monotype and monoprint accept the form of medium 'board' conceptually out of limited condition as mentioned. Thus monotype and monoprint hasn't stand out for several reasons until late 20th century when it started come out to public as people starts to have interests that works from famous artists are actually based on monotype and monoprint. This dissertation likes to step into the monotype and monoprint theoretically which is not well known in domestic and try to study the meaning of monotype and monoprint as a printing medium which is also hasn't been considered sincerely. For this study the process follow as below. First, look into how monotype and monoprint has a concept and history. Next, check differences through comparison with traditional printing and how printing can be understood in what aspects at the same time. After, verify how monotype and monoprint have influence on the acceptance of extended concept of 'board'. This study will show the expressional possibility of monotype and monoprint which has already known as 'tableau printing' in today's situation where adventurous experiments of printing medium are going on with the development of technology.