• Journal of Plant Biotechnology
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• v.46 no.4
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• pp.297-302
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• 2019

Hibiscus syriacus L., the national flower of Korea, is a commonly grown ornamental species found in parks, gardens and along roadsides in Korea. This study sought to establish a plant regeneration system of H. syriacus 'Nanpa' using mature leaves as an explant. Sterilized mature leaf explants were cultured on modified MS medium with combinations of NAA and BAP at various concentrations for 6 weeks. Among the various media evaluated, modified MS media containing 0.46 mg/L NAA and 0.5 mg/L BAP (CI-1) or 0.92 mg/L NAA and 1 mg/L BAP (CI-4) were the most effective for callus formation. Whitish-yellow calluses were observed on CI-1 medium, while green calluses formed on CI-4 medium. The whitish-yellow callus derived from CI-1 medium showed a higher frequency of shoot induction than the green callus derived from CI-4 on modified MS medium containing 0.5 mg/L BAP. Among the various media evaluated in this study, modified MS medium containing 0.46 mg/L NAA and 0.5 mg/L BAP was the most optimal for shoot-forming callus production. Our findings show that mature leaves of H. syriacus 'Nanpa' can be used for in vitro plant regeneration, and the regeneration system described here may be a powerful tool for molecular breeding of H. syriacus 'Nanpa' in the future.

• Journal of Plant Biotechnology
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• v.44 no.3
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• pp.335-342
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• 2017

Purple passion fruit (Passiflora edulis Sims) is one of the introduced tropical plants, an increasing interest has arisen due to its distinctive taste and attractive flavor. It is expected that passion fruit production and planted area will increase gradually in the years ahead because of high profitability and consumer's demands of healthful ingredients. So we tried to investigate the effect of plant growth regulators and antioxidants on in vitro plant regeneration and callus induction from leaf explants of passion fruit for an establishment of optimal mass propagation system. Young leaf explants of purple passion fruit were cultured in Murashige and Skoog (MS) medium containing different growth regulators and antioxidant additives to induce the shoot organogenesis. After 8 weeks, the highest embryogenic callus formation rate was obtained in MS medium supplemented with $1mg{\cdot}L^{-1}$ 6-benzylaminopurine (BAP) and $2mg{\cdot}L^{-1}$ 2,4-dichlorophenoxyacetic acid (2,4-D), furthermore, the shoot development via organogenesis was also observed. Silver nitrate ($AgNO_3$), which was added into the medium to minimize the adverse effects of leached phenolics, was effective for reduction of medium browning and sudden explant death. In the medium supplemented with $1mg{\cdot}L^{-1}$ BAP and $1mg{\cdot}L^{-1}$ gibberellic acid ($GA_3$), shoots were most vigorously regenerated and elongated. Most shoots rooted successfully in half strength medium with $1mg{\cdot}L^{-1}$ indol-3 acetic acid (IAA), and more than 90% of plantlets survived after 4-month acclimatization period.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.24 no.6
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• pp.1309-1318
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• 2014

In the past few years, data leakage of information assets has become a prominent social issue. According to the National Industrial Security Center in South Korea, 71 percent who suffer from technology leakage are small and medium sized enterprises. Hence, establishment and operation of ISMS (Information Security Management System) for small and medium sized enterprises become an important issue. Since it is not easy to obtain ISMS certification for a small or medium sized enterprise by itself, consultation with an expert firm in information security is necessary before the security implementation. However, how to select a proper security consulting company for a small or medium sized firm has not been studied yet. In this study, we analyze empirically the selection factors of ISMS certification consulting company for a small or medium sized firm through exploratory factor analysis (EFA). Our study identified the following four important factors in selecting a security consulting company: expertise of the staffs and human resource management proficiency, market leading capability, competence to make progress during the consultation, and the performance and the size of the physical assets and human resources.

• The Journal of Asian Finance, Economics and Business
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• v.10 no.2
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• pp.301-311
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• 2023

This research aims to identify the role that small and medium enterprises (SMEs) can play in achieving the economic goals of the vision of Saudi Arabia 2030. The study relied on descriptive analysis, designing a standard model, and analyzing it using the Eviews9 program. The study also adopted the questionnaire as a tool for data collection. The study area covered Alkharj and Hawtat Bani Tamim governorates. The sample size of the study was 142 participants. The study's results confirmed the existence of a significant impact of changes in independent variables (X1, X2, X3, X4), which are (GDP, non-oil exports, number of employees, and public revenues), respectively. The dependent variable (Y) represents the number of small and medium-sized businesses in the Kingdom of Saudi Arabia. Additionally, it was found that 61.3% of small and medium-sized enterprises in the governorates of Al-Kharj and Hawtat Bani Tamim operate in the commercial sector. Most study participants concur that SMEs significantly lowered the unemployment rate and helped boost the GDP rate in the Kingdom of Saudi Arabia. The obstacles and difficulties facing the establishment of these enterprises were financial problems, marketing problems, and corporate monopoly. Furthermore, most of the small and medium l enterprises faced financing problems.

• Asian Journal of Turfgrass Science
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• v.15 no.1
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• pp.9-14
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• 2001

Callus formation and plant regeneration from the seeds of zoysiagrass cv. Zenith was tested on MS basal medium supplemented with various concentrations of 2,4-dichlorophenoxyacetic acid(2,4-D) and of several cytokinins. A concentration of 1mg/L 2,4-D on medium stimulated callus formation. In the presence of 5mg/L 2,4-D, addition of 1mg/L kinetin significantly enhanced callus formation and plant regeneration over 2,4-D alone. To transfer foreign DNA into turfgrass, parameters for the bombardment of embryogenic callus with the particle bombardment were partially optimized using transient expression assay of a $chimeric \beta$-glucuronidase(GUS) gene driven by the CaMV 35S promoter. GUS gene was strongly expressed at helium pressure 1,100 psi and 6~9cm target distance.

• Journal of Embryo Transfer
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• v.14 no.1
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• pp.39-46
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• 1999

To establish the optimal culture systems for production of transferable embryos in Korean Cattle, pregnancy rates of IVF-derived blastocysts according to different culture media, culture method and culture duration were compared. Development of IVF-derived embryos to blastocysts was most effective in YS medium group co-cultre with cumulus cells. Blastocysts cultured for 6 to 8 d in vitro showed higher hatching rate and good quality. Pregnancy rates after transfer of IVF-derived blastocysts cultured for 7 or 8 d were high. Through our experiments, it is considered that improvement of culture media and culture method is necessary for mass production of blastocysts with excellent of good quality in Korean Cattle.

• Journal of Plant Biology
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• v.39 no.3
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• pp.161-166
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• 1996

The hairy root cultures of Centella asiatica were established by infection leaf explants with Agrobacterium rhizogenes A4, 15834 in 1/2 Murashing and skoog liquid medium supplemented with 50 $\mu$M acetosyringone. The induced hairy roots were subjected to paper electrophoresis for the detection of opine and opine-positive clones which were considered to have been transformed. Five hairy root clones were selected according to the different bacterial strains used, growth rate and pattern. Among media tested, MS basal medium substituted phosphate concentration by 2.5mM K2HPO4 showed the highest growth rate in the dark condition.

• Journal of Forest and Environmental Science
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• v.32 no.4
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• pp.392-399
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• 2016

This review paper presents guidelines which stakeholders use in addressing on-farm tree planting configuration, establishment, tending, silvi- cultural management, management of pests and diseases, challenges and opportunities as practiced in the medium to high potential areas of Kenya. The tree planting configurations discussed includes blocks planting (woodlot), boundary, compound planting, home/fruit gardens, trees intercropped or mixed with pasture, trees on riverbanks and roadside. Participatory monitoring and evaluation techniques have been highlighted. The main challenges facing tree planting activities include culture and attitude of local people, land and tree tenure, inadequate technical support, lack of recognition and integration of technical information and indigenous knowledge, capital and labour shortages, lack of appropriate incentives measures, damage by domestic and wild animals, conflict over trees on the boundary and policy and legal issues. This guideline targets forest managers, extension agents, students and other practitioners in policy and day to day decision making processes in Kenya.

• 한국벤처창업학회:학술대회논문집
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• 2006.11a
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• pp.141-157
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• 2006

Ten years have passed since the establishment of KOSHBA (Korea Small and medium enterprises Hybrid Business Association). KOSHBA is composed of over 400 regional groups; over 4,000 enterprises are participating in the association. This paper deals with a survey on the present status of the participating companies in HBA of Kangwon province including the motivation of participation, participating period, and the purpose of participation. The survey results show that they are participating in HBA for manpower interchange, information gathering, mutual utilization of management resources, and their company's growth. To become a dynamic and vital association, it is necessary to share, learn, and discuss their managerial problems based on mutual trust and respect at the regular monthly meeting.

• Proceedings of the Safety Management and Science Conference
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• 2006.11a
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• pp.269-278
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• 2006

Ten years have passed since the establishment of KOSHBA(Korea Small and medium enterprises Hybrid Business Association), KOSHBA is composed of over 400 regional groups; over 4,000 enterprises are participating in the association. This paper deals with a survey on the present status of the participating companies in HBA of Kangwon province including the motivation of participation, participating period, and the purpose of participation. The survey results show that they are participating in HBA for manpower interchange, information gathering, mutual utilization of management resources, and their company's growth. To become a dynamic and vital association, it is necessary to share, learn, and discuss their managerial problems based on mutual trust and respect at the regular monthly meeting.