• 한국식품영양과학회지
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• 제23권5호
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• pp.845-848
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• 1994

The fermenting conditions for acetic acid production with Acetobacter sp. FM-10 which could grow in the medium containing 10% ehtanol were investigated. Initial concentration of acetic acid in broth medium affected greatly to the fermentation speed. For example , the acetic acid production increased proportionally by the increasing of initial concentration was higher that 1.0%. When the cultivation was started with broth medium containing 5% ethanol, the additional adding ethanol during the fermentation was not significantly increased the acidity of the medium. The acidity of the medium containing 10% ethanol was reached to 8.3% after shaking than static cultivation by about 10 days with 150 rpm shaking speed. Acetic acid production with shaking cultivation was faster the static cultivation by abot 10 days under the same condition except shaking. In acetic acid fermentation with the batch style fermentor , the optimum fermentation condition was 700 rpm of agitation speed and 5L/min air flow rate in 3L culture medium .

• Reproductive and Developmental Biology
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• 제29권4호
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• pp.241-245
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• 2005

Porcine oviduct epithelial cells (POEC) are widely used in co-culture experiments to improve early embryonic development, in vitro fertilization in embryo transfer programs for domestic animals and in vitro maturation of immature germ cells. POEC were mechanically isolated and cultured in tissue culture medium 199. Cells grew continuously, and confluent monolayers were formed after 7 days. After forming confluent monolayer of epithelial cells, supernatant was collected as the condition medium for maturing round spermatids in vitro. Round spermatids were also separated mechanically and cultured in the POEC condition medium. In this study we observed that $20\%$ of round spermatid cultured were matured into elongating spermatid after 24 h, and about $10\%$ of round spermatid cultured showed complete elongation (elongated spermatid) within $24\~48$ h of in vitro culture. No further development was observed within $50\~72$ h and transformed cells lost their viability after 72 h. These preliminary findings suggest that the condition medium from POEC may be possible to overcome the round spermatid block by improving the milieu of culture system.

• Journal of Microbiology and Biotechnology
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• 제23권1호
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• pp.47-55
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• 2013

Metabolic alterations of Cordyceps bassiana mycelium were investigated under the following culture medium and light conditions: dextrose agar supplemented with 0.5% yeast extract (SDAY) medium with light (SL), SDAY medium without light (SD), nut medium without light (ND), and iron-supplemented SDAY medium without light (FD). The levels of asparagine, aspartic acid, glutamic acid, glutamine, histidine, lysine, ornithine, and proline were significantly higher under SD and SL conditions. The levels of most of the alcohols, saturated fatty acids, unsaturated fatty acids, fatty acid esters, sterols, and terpenes were higher under the ND condition than in the other conditions, but beauvericin was not detectable under the ND condition. The FD condition was favorable for the enhanced production of aminomalonic acid, malic acid, mannonic acid, and erythritol. Thus, the metabolic characteristics of C. bassiana can be manipulated by varying the cultivation conditions, rendering this fungus potentially favorable as a nutraceutical and medicinal resource.

• 대한전자공학회논문지
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• 제25권9호
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• pp.1108-1114
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• 1988

Usng the P.Yeh's analytic method, we derive the condition for oscillation of a linear phase conjugate oscillator which consists of photorefractive medium and two conventional mirrors. From this general oscillation condition, we obtain the threshold oscillation conditon of a single phase conjugate resonator and the self-oscillation condition of photorefractive medium, then in special case (phase shift =90\ulcorner: no external dc electric field), oscillation conditions of the linear phase conjugate oscillator for any cavity length are derived. The results indicate that, unlike in a phase conjugate oscillator with Kerr-like medium, oscillation cannot occur at special cavity length for given couplinyg strengths.

• 펄프종이기술
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• 제31권4호
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• pp.69-75
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• 1999

This study was performed to investigated the application of mixed resins for corrugated container board. The corrugated container board yields a sandwich structure in which a linerboard material is glued to a corrugated medium . Now, manufacturing corrugated container boards don't provide sufficient strength, and result in box failure during shipping . Therefore improvement of box strength is necessary . In this study, we intend to improve box strength by improving corrugated medium strength with mixed resins and to find the optimum treatment condition of this resins. First, we tried to mixed resins as Starch+CMC, Starch_Irea, CMC+Urea, Second, investigated to applicability of this resins for corrugated medium , and the third, measured tensile index, burst index, and edgewise compression index on liner, medium paper, and single faced corrugated container board. In this test results, we obtained that the improvement ratios of tensile index in liner and medium paper were approximately 80-185%, 60-118% , respectively. The respecting improvement ratios of edgewise compression index of single faced corrugated container board was approximately 91-124%, relatively. In addition, we concluded that optimum condition in mixing ratio was 1 :3 with CMC + Urea and the ap[plication amounts was 9% on materials. Fro manufacture of corrugated container board, optimum condition in mixing ratio was 1 : 3 with 5% CMC +Urea , because of considering to improvement of strength on cost.

• Biotechnology and Bioprocess Engineering:BBE
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• 제2권2호
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• pp.82-85
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• 1997

The nar promoter as an inducible promoter was characterized for the process development for the gene expression and the protein production under anaerobic condition. The LB medium was selected as a main culture medium showing the enzyme activity of 18,000 units/min/g cell in the flask cultivation. The optimum concentration of nitrate was 1%. Under anaerobic conditions, the gene expression was fully induced in the presence of nitrate.

• 대한기계학회논문집B
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• 제24권12호
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• pp.1635-1643
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• 2000

The present work investigates a heat transfer phenomenon at the interface between a porous medium and an impermeable wall. In an effort to appropriately describe the heat transfer phenomenon at the interface, the heat transfer at the interface between the microchannel heat sink, which is an ideally organized porous medium, and the finite-thickness substrate is examined. From the examination, it is clarified that the he heat flux distribution at the interface is not uniform for the impermeable wall with finite thickness. On the other hand, the first approach, based on the energy balance for the representative elementary volume in the porous medium, is physically reason able. When the first approach is applied to the thermal boundary condition, and additional boundary condition based on the local thermal equilibrium assumption at the interface is used. This additional boundary condition is applicable except for the very th in impermeable wall. Hence, for practical situations, the first approach in combination with the local thermal equilibrium assumption at the interface is suggested as an appropriate thermal boundary condition. In order to confirm our suggestion, convective flows both in a microchannel heat sink and in a sintered porous channel subject to a constant heat flux condition are analyzed. The analytically obtained thermal resistance of the microchannel heat sink and the numerically obtained overall Nusselt number for the sintered porous channel are shown to be in close agreement with available experimental results when our suggestion for the thermal boundary conditions is applied.

• 한국안전학회지
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• 제14권2호
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• pp.140-147
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• 1999

Currently, the consumer pattern is directed to small quantity and high variety these days. World market is led by technological innovations and changes rapidly. To survive in this environment, the raising of small and medium enterprises is highly desirable. In the context of nationwide restructuring, competitive medium and small industries which are in good financial status should lead the nation's economy. But general ignorance of the safety issues could lead to a miss management of this industry in this field. In this study, actual condition and improvement of safety management are as follows; We have to 1) find out the factors which endangers this industry, and set a proper guideline for safety management. 2) encourage safety management of these industries by themselves, and advertise and provide technical support for using PC and internet. 3) set a higher standard of safety issue when accrediting a prospective medium and small industry, and have a firm control of this issue once accredited. 4) supply more governmental financial aids to terms of safety issues for medium and small industries.

• 한국미생물·생명공학회지
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• 제26권2호
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• pp.130-136
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• 1998

참당귀 세포(Angelica gigas H4)를 현탁배양하여 면역 증강성 다당을 생산하기 위한 최적배지 설계 및 생산조건 최적화 연구를 수행하였다. 우선 세포의 성장에 가장 좋은 배지로서 Schenk and Hildebrandt(SH) 배지를 선택하여 배양한 결과 $25^{\circ}C$, 암배양조건에서 각각 15.8g DCW/l와 0.85 g/l의 세포 및 다당을 얻을 수 있었다. 한편 다당 생산용 배지의 개발을 위하여 여러 가지 식물세포배양용 배지 중에서 다당 생산능이 가장 높은 배지로서 Gamborg B5 배지를 선정하였으며 B5 배지를 변형하여 면역증강성 다당생산을 위한 최적배지(PPM)를 개발하였다. 그리고 최적배지를 이용한 당귀 세포증식과 다당 생산을 위한 최적 pH는 6.0-6.5, 배양온도는 $20^{\circ}C$, 그리 고 광조건보다는 암조건이 좋은 것으로 밝혀졌다. 상기 최적조건에서의 최대 세포증식과 다당생산은 각각 14.8 g/l, 1.5 g/l 이었다.

• 식물조직배양학회지
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• 제28권6호
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• pp.305-310
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• 2001

본 실험은 지치 (Lithospermum erythrorhizon)의 뿌리조직으로부터 유도한 캘러스에 ${\gamma}$선을 조사하여 배양기간과 배지조성 및 명.암배양의 조건에 따라 ${\gamma}$선 조사가 캘러스 생장촉진 효과가 있는지를 검토하고자 수행하였다. 지치의 캘러스 생장은 전체적으로 암배양에서보다 명배양에서 효과적이었다. r선 조사에 의한 효과에 있어서는 LLB2N2배지의 8 Gy에서 캘러스의 생장에 효과가 가장 좋았고, LLK2I0.2배지에서는 16 Gy에서 캘러스 생장효과가 좋았지만 암배양에서의 ${\gamma}$선처리 효과는 없었다. 명배양에서 LS배지에 BA 1 mg/L와 NAA 1 mg/L를 첨가할 경우, BA 2mg/L와 NAA 2 mg/L를 첨가할 때보다 캘러스의 생장효과가 있었으나, 암배양에서는 차이가 없었다. 특정선량에 대한 BA와 NAA의 농도에 있어서는 각각 2 mg/L가 효과적인 것으로 나타났다. MK2I0.2배지는 0.5 Gy와 1 Gy에서 캘러스의 생장에 효과적이었으나, 기본배지의 종류에 있어서는 MS배지보다 LS배지에서 더 효과적이었다. M-9배지에서는 명배양에서도 캘러스의 생장은 저조하였으나, 16 Gy 및 30 Gy에서 캘러스의 생장이 30%이상 증가하였다. 암배양에서는 ${\gamma}$선 조사에 의한 캘러스의 생장효과는 거의 없었다.