• Title/Summary/Keyword: medium composition

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Gene Cloning of Cellulose Degradation Enzyme of Bacillus subtilis LYH201 Strain (Bacillus subtilis LYH201균주의 섬유소 분해효소의 유전자 Cloning 및 특성분석)

  • Lee, Young-Han;Park, Sang-Ryeol
    • Korean Journal of Soil Science and Fertilizer
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    • v.34 no.5
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    • pp.333-341
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    • 2001
  • The Compost-decomposing-bacteria was isolated from livestock compost containing sawdust. The isolated bacteria was identified as Bacillus subtilis LYH201 by the method of the composition of the fatty acid with MIDI system and Bergey's manual. Cloning of CMCase encoding gene was accompanied by shotgun method. The pLK100 have yellow activity ring on CMC medium, that was carried 2.2 kb insert DNA in pBluescript II $SK^+$ vector, named BglC gene. The BglC was very similar to Pectobacterium carotovorum Gun_CLOAB(P15704) with score of 57% identity and 71% homology over 508 aa. The BglC was measured molecular weight 56 kDa by CMC-SDS-PAGE. Optimum cellulase activity Bacillus subtilis LYH201 was temperature $50^{\circ}C$ and pH 7.

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Mineralogy of Clinopyroxene from the Geodo Mine (거도광산의 단사휘석에 관한 광물학적 연구)

  • 최진범;김수진
    • Journal of the Mineralogical Society of Korea
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    • v.2 no.1
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    • pp.26-36
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    • 1989
  • Clinopyroxene in the Geodo mine belongs to diopside-hedenbergite series. It is widely distributed throughout the mine area together with garnet and is also closely related with Fe-mineralization. Clinopyroxenes in the Geodo mine including two samples from the sangdong and Ulchin Mines are studied using polarized microscope, EPMA, XRD, and IR spectroscopy for occurrence, chemistry, structure, and crystal chemistry. Especially, variations in unit-cell parameters are examined in relation with the substitution scheme between Fe and Mg cations. Clinopyroxenes in the Geodo mine occur in both endoskarn and exoskarn zone. It is mostly anhedral to subhedral with fine- to medium-grained in texture, but some have bigger crystals of short prismatic or columnar habits. Clinopyroxene occurs as monomineralic or is associated with mostly garnet and sometimes with actinolite, magnetite, epidote, and chlorite. Chemical analysis reveals that the Geodo clinopyroxene is diopsidic in composition (Di: 65-96%). This fact is in good contrast with garnet chemistry showing mostly andraditic (An: 41-82%). Especially, clinopyroxene coexisting with magnetite belongs to nearly end member diopside (Di: 97-99%). Thus, diopside-andradite pair indicates that Geodo skarns were formed under the reduced environment. X-ray diffraction analysis shows unit-cell parameters vary with increase of Fe contents: a = 9.765-9.838$\AA$, b = 8.943-9.020$\AA$, c= 5.240-5.253$\AA$.$\beta$ = 105.70-104.83$^{\circ}$, and V =440.64-448.19$\AA$3. It is noted from the least square regression that a, b and V increase linearly with increase of Fe content, while $\beta$ slightly decreases and c remains nearly unchanged as change in Fe content. These trends are to difference between synthetic and natural clinopyroxenes. This fact is also recognized in IR spectra which show a slight shift of several absorption bands toward lower wavenumber region with increasing Fe content.

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Food Intake Patterns and Blood Clinical Indices in Female College Students by HDL-cholesterol Levels (여대생의 HDL-콜레스테롤 수준에 따른 임상건강지표와 영양섭취 실태)

  • Jung, Ha-Na;Kim, Jung-Hee
    • Korean Journal of Community Nutrition
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    • v.13 no.1
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    • pp.100-110
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    • 2008
  • Recently, a lot of epidemiological studies revealed that low HDL-cholesterol level was a better predictor of risk for coronary heart disease than total cholesterol. This study investigated the anthropometric parameters, clinical blood indices, and dietary factors influencing serum HDL-cholesterol level by using a cross-sectional study for Korean female college students. The subjects were 94 female college students. They were divided into three groups according to their serum HDL-cholesterol levels, low HDL-cholesterol (<50 mg/dL, n=20), medium HDL-cholesterol (50 $\leq$, < 60 mg/dL, n=39) high serum HDL-cholesterol groups (60 $\leq$ mg/dL, n = 35). This study examined their demographic data and dietary intake throughout a questionnaire. Clinical blood indices were measured using an automatic blood chemistry analyzer (Selectra E), after 12 hours of fasting. BMI, body weight, fat mass, and waist circumferences were significantly increased according to low serum HDL-cholesterol levels. Serum lipid analysis showed a significantly higher level of TG, LDL-/HDL-Ratio, atherogenic index in the low HDL-cholesterol group. Serum levels of GPT, uric acid and alkaline phosphatase in the low HDL-cholesterol group were significantly higher than in the other group. The average consumption of energy was 1627 kcal and 77.76% of estimated energy requirement (EER). The mean ratio of calories from carbohydrate: protein: fat was 57:15:28. The low HDL-cholesterol group was significantly higher than the other groups in eggs, fat and oils consumption. Interestingly, milk and diary products consumption of low HDL-cholesterol group was half (p < 0.05) of those of the other groups. In conclusion, serum HDL-cholesterol levels appeared to be decreased by increasing BMI, fat mass, waist circumference, and serum TG level. In addition, some dietary factors seemed to be related to serum HDL-cholesterol levels. However, further research is needed to elucidate the exact relationship between serum HDL-cholesterol level and dietary factors.

Beach Sediments of the Jeju Island, Korea (제주(濟州) 연안(沿岸)의 해빈퇴적물(海濱堆積物)에 관(關)한 연구(硏究))

  • Youn, Jeong-Su
    • Economic and Environmental Geology
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    • v.18 no.1
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    • pp.55-63
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    • 1985
  • Studies based on field observation and laboratory analysis of the littoral sands of beaches in Jeju Island indicate that the shores exhibit a great variation in both the beach geometry and the composition in terms of geological agents. Most of the beaches around the Island are developed in relatively small patches and discontinuous, as the result of intervening sea cliffs and rocky headlands. The sand quality and the dimensions of the beaches in the Island are relatively poor; for example, these beaches are 220~2,780m in length, 41~313m in width, and $7^{\circ}$ steepness in average foreshore slope. According to the textural parameters analysis, the beach sediments in study portion shows medium grained ($average\;1.42{\phi}$), moderately well sorted ($average\;0.65{\phi}$) and negative skewed ($average\;0.34{\phi}$), which seems to reflecting a high energy marine depositional environment. The heavy and light minerals of te beach sediments are composed of quartz, volcanic fragments, Na-Ca feldspar, olivine, augite as major constituents, along with apatite, biotite and other minor components, which originates from the adjacent geology. The content of CaO-MgO in shell fragments of the littoral sands ranged from 4.69~51.96%, suggesting that the high CaO-MgO content in some of the Island's beaches is attributable to geologic environments conducive to the growth of shell organisms and sediments migration. The provenance of the sediments studied are derived predominantly from adjacent continental shelves and/or terrigenous older river portion, Which sediments were transported mostly by rolling or bottom suspension. The depositional environments of the Jeju beaches can be divided into two types: beaches distributed in the North and the Northeastern parts of the Island are dominated by marine enviroment, whereas beaches in the Southwestern portion are characterized by terrigenous agencies.

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Wallrock Alteration and Primary Dispersion of Elements in the Vicinity of the Mugeug Gold-bearing Quartz Veins (무극 함금석영맥광상 주변모암에서의 모암변질과 원소들의 일차분산)

  • Hwang, In Ho;Chon, Hyo Taek
    • Economic and Environmental Geology
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    • v.27 no.4
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    • pp.387-396
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    • 1994
  • Mineralogical and geochemical studies on gold-bearing quartz veins and wallrock from the Mugeug mine were carried out in order to investigate the variation of mineralogical composition and the geochemical behavior of elements with distance from the gold-bearing quartz veins. Gold-bearing quartz veins occur in early Cretaceous medium- to coarse-grained biotite granite. The unaltered wallrock is composed mainly of quartz, plagioclase, orthoclase, microcline, biotite and hornblende with accessory minerals of sphene and apatite. Mineralogical changes in altered wallrock around the gold-bearing quartz veins were observed as follows; 1) biotite and hornblende altered into chlorite, and next to sericite, 2) plagioclase, orthoclase and microcline altered into sericite, and 3) calcite and quartz introduced into wallrock. Contents of $K_2O$, Rb, Cs, Au, As and Sb in altered wallrock increase, whereas those of $Na_2O$, CaO, Ba, and Sr decrease with proximity to the gold-bearing quartz veins. The loss on ignition also increases with the increase of alteration mineral. The width of primary dispersion increases in order $Au=SiO_2<As=Cs=Rb<K_2O=Sb$ and $MnO<Na_2O=CaO=Ba<Sr$. The sericitization index, $K_2O/(K_2O+Na_2O)$, is an important indicator to interpret the degree of alteration at the Mugeug mine, which is more than 0.8 in strongly and moderately altered granite, 0.5~0.8 in wea altered granite, and less than 0.5 in unaltered granite. Alteration indices for major and trace elements, and the ratio of Rb/Sr are also useful to discriminate alteration zones.

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Development of Optimal Thermal Transfer Calculation Algorithm by Composition of Thermal Transfer Mechanism among Integrated Energy Operators (집단에너지 사업자간의 열연계 메커니즘 구성에 의한 최적 열연계 산정 알고리즘 개발)

  • Kim, Yongha;Kim, Seunghee;Hyeon, Seungyeon
    • Journal of Energy Engineering
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    • v.26 no.4
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    • pp.57-66
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    • 2017
  • Since the heat is not as fast as the electric power and the loss is relatively large compared to the electric power, it is not realistic to operate the thermal transfer system with on operation center like electric power trading. In the case of the Korea District Heating Corporation, where all the thermal transfer are currently being made, only two or four adjacent heat-generating power plants are being the heat trading. Therefore, In this paper, we concluded that it is appropriate to divide the integrated operation center for heat trading into several regions, to operate the hub integrated operation power plant in each region to reflect the characteristics of the heat medium and proposed the thermal transfer mechanism among integrated energy operators. Then, we have developed an algorithm that can optimize the heat transaction for the proposed mechanism and applied it to the actual operators to verify the usefulness of the proposed algorithm.

PHYSICAL PROPERTIES OF THE GIANT H II REGION G353.2+0.9 IN NGC 6357

  • BOHIGAS JOAQUIN;TAPIA MAURICIO;ROTH MIGUEL;RUlZ MARIA TERESA
    • Journal of The Korean Astronomical Society
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    • v.37 no.4
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    • pp.281-284
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    • 2004
  • Optical imaging and spectroscopy of G353.2+0.9, the brightest part of the giant H II region NGC 6357, shows that this H II region is optically thin, contains ${\~}300\;M_{\bigodot}$ of ionized gas and is probably expanding into the surrounding medium. Its chemical composition is similar to that found in other H II regions at similar galactocentric distances if temperature fluctuations are significant. The inner regions are probably made of thin shells and filaments, whereas extended slabs of material, maybe shells seen edge-on, are found in the periphery. The radio continuum and H$\alpha$ emission maps are very similar, indicating that most of the optical nebula is not embedded in the denser regions traced by molecular gas and the presence of IR sources. About $10^{50}$ UV photons per second are required to produce the H$\beta$ flux from the 1l.3'${\times}$10' region surrounding the Pis 24 cluster that is south of G353.2+0.9. Most of the energy powering this region is produced by the 03-7 stars in Pis 24. Most of the 2MASS sources in the field with large infrared excesses are within G353.2+0.9, indicating that the most recent star forming process occured within it. The formation of Pis 24 preceded and caused the formation of this new generation of stars and may be responsible for the present-day morphology of the entire NGC 6357 region.

Comparison of β-glucan Contents of Lentinula edodes Cultivated on Sawdust according to Medium Composition and Fruiting Temperature (표고 톱밥재배에서 배지조성과 버섯발생 온도에 따른 β-glucan 함량 비교)

  • Park, Young-Ae;Bak, Won-Chull;Ka, Kang-Hyeon;Koo, Chang-Duck
    • The Korean Journal of Mycology
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    • v.44 no.4
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    • pp.296-299
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    • 2016
  • Lentinula edodes is an edible mushroom that contains a ${\beta}$-glucan called lentinan, which has antitumor and immune-enhancing properties. In the present study, the ${\beta}$-glucan contents of L. edodes mushrooms cultivated on sawdust with different nutritional supplements and fruiting temperatures were measured using a commercial ${\beta}$-glucan assay kit purchased from Megazyme (Bray, Ireland). The weight loss of sawdust media and the yield of fruiting bodies showed similar trends, but the yield was more closely associated with the nutritional supplements used than the weight loss of sawdust media was. The ${\beta}$-glucan contents of L. edodes were 39.5-42.1%, except in the bean curd refuse + $CaCl_2$ supplementation group (50.4%). Furthermore, the ${\beta}$-glucan content decreased with increasing temperatures and was 42.4% at a low fruiting temperature.

Interpretation of Provenance and Transportation Process for Bakseok of Geunjeongjeon Hall in Gyeongbokgung Palace, Korea (경복궁 근정전 박석의 산지와 운송과정 해석)

  • Choie, Myoungju;Lee, Chan Hee;Jo, Young Hoon
    • The Journal of the Petrological Society of Korea
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    • v.24 no.3
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    • pp.181-191
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    • 2015
  • The Bakseok of Geunjeongjeon Hall in Gyeongbokgung Palace is platy floor stone acting as drainage, protected glaring and sliding. The Bakseoks were composed of anisotropic medium-grained biotite granite with a weak myrmekitic texture. Interpretation of transportation process for the Bakseok and original granite provenance trace of and analyze of identity based on ancient writings and detail field survey. As a result, the very similar granite with the Bakseok in lithology, composition mineral, texture and geochemical characteristics was found around Mt. Nakgasan in the Seokmodo Island. There were interpreted stonework process of the Bakseok used exfoliation granite dome with physical weathering properties in Seokmodo Island, to get platy stone economically stonework at the state level as a minimized on burden of supply and men power.

Relationship Between Dissolution Patterns of Carbamazepine Tablet and Dissolution Medium Composition (카르바마제핀 정제 용출패턴과 용출액 조성과의 상관성)

  • Lee, Hyeon-Tae;Kim, Jeong-Ho;Kim, Hyun-Joo;Sah, Hong-Kee
    • Journal of Pharmaceutical Investigation
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    • v.34 no.3
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    • pp.185-192
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    • 2004
  • The objective of this study was to evaluate the effects of surfactant type and concentration upon dissolution rates of carbamazepine from an immediate-release tablet. The dissolution media used in this study were aqueous solutions containing 0.1-2% sodium lauryl sulfate, cetyltrimethylammonium bromide, or polysorbate 80. The solubility of carbamazepine in the dissolution media was determined at first. A dissolution study was then conducted by using the USP dissolution apparatus II (paddle method) with an agitation rate of 75 rpm. Aliquots of the dissolution media were taken at predetermined time intervals, and the amount of carbamazepine dissolved was measured spectrophotometrically at 285 nm. The dissolution data obtained were fitted into a biphasic exponential equation with four parameters. Excellent correlations were observed between the experimental data and the theoretical ones predicted by the equation. This equation permitted the calculation of $T_{50%}$ (the time required for dissolving 50% of carbamazepine) under various experimental conditions. Differentiation of the equation also led to the attainment of dissolution rates at dissolution time points. The addition of a surfactant to an aqueous solution led to increasing the solubility of carbamazepine by 3- to 12-folds, depending upon its type and concentration. This event also resulted in enhancing the magnitude of a sink condition during the dissolution study. As a result, the dissolution rate of carbamazepine was affected by the aqueous surfactant concentration in a proportional manner. Subsequently, $T_{50%}$ values declined rapidly, as the surfactant concentration increased. Such effects were observed in decreasing order of sodium lauryl sulfate, cetyltirmethylammonium bromide, and polysorbate 80. These results clearly demonstrated that it was possible to tailor a dissolution rate and $T_{50%}$ of carbamazepine by manipulating the type and concentration of a surfactant. Relevant information would be beneficial to setting up dissolution specifications for poorly water-soluble drug products.