• Korean Journal of Fisheries and Aquatic Sciences
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• v.50 no.6
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• pp.637-649
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• 2017

Lipid levels and fatty acid compositions of 20 species of subtropical fish caught off the coast of Jeju Island, Korea were determined. The fish were categorized according to total lipid (TL) content as follows; lean fish ($TL=1.19{\pm}0.32%$, n=10 species), low-fat fish ($TL=3.02{\pm}0.44%$, n=6 species) and medium-fat fish ($TL=6.87{\pm}1.23%$, n=4 species). The prominent fatty acids were saturated fatty acids (SFAs), at 16:0 and 18:0 monounsaturated fatty acids (MUFAs), at 18:1n-9 and 16:1n-7, and polyunsaturated fatty acids (PUFAs), at 22:6n-3, 20:5n-3 and/or 20:4n-6. Across all species, the percentages of PUFAs and SFAs were $38.0{\pm}7.89%$ and $34.3{\pm}3.08%$, respectively, with no significant difference between both groups (P<0.05), but showed higher proportions than that of MUFA group ($27.8{\pm}7.23%$) (P<0.05). The proportion of n-3 PUFAs was $27.1{\pm}8.68%$ across all fish species, similar to levels in other fishes from Korea. These results suggest that subtropical fish from Jeju Island are a good source of n-3 PUFAs for humans.

• Microbiology and Biotechnology Letters
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• v.11 no.3
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• pp.217-222
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• 1983

By the treatment of Gibberella fujkuroi I-892 with mutagen such as UV light and N-methyl-N'-nitro-N-nitrosoguanidine, a mutant G. fujkuroi G-471 was selected as the highest producer of gibberellic acid among 800 mutant strains. It showed 30% increase of production yield compared with that of the parent strain. At optimum medium composition (saccharose 1.0%, ammonium tartarate 50mM, malt extract 1.0% KH$_2$PO$_4$ 0.5%, MgSO$_4$0.5%, FeSO$_4$0.0002%, trace element sol.0.002% (v/v), the yield of submerged culture increased by 30% after 7 days culture at 24$^{\circ}C$ (253mg/$\ell$). In submerged culture, the initial pH showed much effects on the increase of gibberellic acid production. The highest yield of the production was attained with pH adjustment to 4.0 at the initial stage of fermention.

• Asian-Australasian Journal of Animal Sciences
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• v.28 no.6
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• pp.816-826
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• 2015

The present experiment evaluated the influence of moisture level and anaerobic fermentation on aerobic stability of total mixed ration (TMR). The dynamic changes in chemical composition and microbial population that occur after air exposure were examined, and the species of yeast associated with the deterioration process were also identified in both non-fermented and fermented TMR to deepen the understanding of aerobic deterioration. The moisture levels of TMR in this experiment were adjusted to 400 g/kg (low moisture level, LML), 450 g/kg (medium moisture level, MML), and 500 g/kg (high moisture level, HML), and both non-fermented and 56-d-fermented TMR were subjected to air exposure to determine aerobic stability. Aerobic deterioration resulted in high losses of nutritional components and largely reduced dry matter digestibility. Non-fermented TMR deteriorated during 48 h of air exposure and the HML treatment was more aerobically unstable. On dry matter (DM) basis, yeast populations significantly increased from $10^7$ to $10^{10}cfu/g$ during air exposure, and Candida ethanolica was the predominant species during deterioration in non-fermented TMR. Fermented TMR exhibited considerable resistance to aerobic deterioration. Spoilage was only observed in the HML treatment and its yeast population increased dramatically to $10^9cfu/g$ DM when air exposure progressed to 30 d. Zygosaccharomyces bailii was the sole yeast species isolated when spoilage occurred. These results confirmed that non-fermented and fermented TMR with a HML are more prone to spoilage, and fermented TMR has considerable resistance to aerobic deterioration. Yeasts can trigger aerobic deterioration in both non-fermented and fermented TMR. C. ethanolica may be involved in the spoilage of non-fermented TMR and the vigorous growth of Z. bailii can initiate aerobic deterioration in fermented TMR.

• Asian-Australasian Journal of Animal Sciences
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• v.27 no.6
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• pp.825-831
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• 2014

The aim of this study was to determine the nutritive value of some legume species in salt-affected soils of South-East Anatolian region using chemical composition and in vitro gas production kinetics. In this study, Lotus corniculatus, Trifolium alexandrinum, Medicago sativa were sown and tested in four different locations. A 3 by 4 factorial design with 3 legume species and 4 salt levels (non salty electrical conductivity (EC)<4 dS/m; low salt: 4 dS/m>EC<8 dS/m, medium saline: 8 dS/m>EC<16 dS/m and high salt: 16 dS/m>EC) was used in the study. Results indicated that salinity and plants had no significant effect on ash and ether extract. Dry matter (DM), acid detergent fiber, digestible dry matter, dry matter intake (DMI) were affected by plant, salinity and plant${\times}$salinity interaction. On the other hand neutral detergent fiber, relative feed value (RFV), and DMI were affected by salinity and plant${\times}$salinity interaction. Mineral contents were affected by plant species, salinity and salinity${\times}$plants interactions. In vitro gas production, their kinetics and estimated parameters such as were not affected by salinity whereas the gas production up to 48 h, organic matter digestibility, metabolizable energy (ME), and net energy lactation ($NE_L$) were affected by plant and plant${\times}$salt interaction. Generally RFVs of all species ranged from 120 to 210 and were quite satisfactory in salty conditions. Current results show that the feed value of Medicago sativa is higher compared to Lotus corniculatus and Trifolium alexandrinum.

• KSBB Journal
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• v.8 no.2
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• pp.172-177
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• 1993

In order to reduce BOD of wastewater from alcohol distillery manufactory, the production of single cell lipid was attempted. Among five yeast strains tested, Rhodotorula glutinis was most desirable for lipid production. Wastewater was treated with 2N NaOH and used as a medium. The optimum pH and temperature for lipid production were found to be 5.0 and $30^{\circ}C$, respectively. The addition of monobasic phosphate was good for cultivation of Rhodotorula glutinis. The C/N ratio was an important factor for lipid production and composition. The best C/N ratio was 50 for the production of single cell lipid. By cultivation Rhodctorula glutinis for 4 days, 4g/L of single cell lipid was harvested and BOD of wastewater reduced by 88.7%.

• Journal of Distribution Science
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• v.14 no.10
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• pp.153-164
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• 2016

Purpose - In general, large companies have larger organizations, funds, and systems to provide more effective and efficient services in the market. However, development needs to support the livelihood of ordinary citizens who work for small businesses as well. This research suggests that a new distribution channel, called a "foothold style Kosa mart," that cooperates jointly with a distribution center and a large discount mart can provide direct solutions to small and mid-size distributors. This new distribution channel can achieve a limited type of "Nadle shop (small supermarket) foster project" related to building a joint distribution center and improvement in wholesale supply. Research design, data, and methodology - Data about the Korea distribution situation, the Nadle stores, and the logistics centers were collected from literature, Statistics Korea, journals, and reports. Specifically, we investigated information about Kosa Mart and Nadle stores. We focused on the redesign of the distribution center for the Nadle store. Results - The Kosa Mart distribution center now includes 18 warehouses, and has been handling 2000-3000 items. Most of the warehouses have been simply designed and items loaded and stored without refrigeration; thus, it is possible to store only products of certain manufactured goods. The current logistics center has no wholesale function because it failed to resolve the joint purchasing and product supply issues of competitively priced products. Conclusions - This study aimed to identify ways to strengthen the competitiveness of small- and medium-sized retailers. A Kosa Mart redesign aims to unifying the logistics center, stores, and customers. First, the joint wholesale logistics system, equipped with an integrated ordering system, needs to process customer orders and store orders at the same time. Second, excellent small business product development has to connect with production. Third, the store composition needs to support a shipping hub. Fourth, the Mart differentiates itself from convenience store goods by supplying regional and specialized products to customers. Fifth, a service buying agent and direct transactions between producers and consumers need to be established, and exhibits and displays of goods need to be improved.

• Microbiology and Biotechnology Letters
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• v.18 no.3
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• pp.273-279
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• 1990

For poly- $\beta$ -hydroxybutyrate (PHB) production, a pink-pigmented facultative methylotrophic bacterium (PPFM) P-10 was newly isolated from soils through methanol-enrichment culture. The optimal medium composition for cell growth was 1.0% (vlv) of methanol as carbon source and l.Og/l of ,TEX>$NH_4Cl$, equivalent to C/N ratio of 13.2 at pH 7.0 and $30^{\circ}C$. To investigate the optimal condition for YHB accumulation, two-stage culture technique was adopted; first stage for cell growth and second stage for accumulation of PHB providing unbalanced growth conditions. The optimal PHB accumulation was 1.0% (vIv) of methanol and 0.26gll of $NH_4Cl$, C/N of 50.8 at pH 6.0. To overcome methanol inhibition on cell growth, intermittent feeding fed-batch culture technique was employed, and the cell concentration as high as 14gll with 40% of PHB was achieved. The purified PHB was identified using IR and $1^H NMR$ as homopolymer of 8hydroxybutyric acid. The absorption spectrum of extracted pink colored microbial pigment was alsa investigated.

• Current Research on Agriculture and Life Sciences
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• v.31 no.1
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• pp.30-37
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• 2013

Bacillus subtilis and Bacillus amyloliquefaciens are closely related species that share a similar genomic background, and are both known to secrete large amounts of proteins directly into a medium. The extracellular proteomes of two strains of Bacillus subtilis and two strains of Bacillus amyloliquefaciens were compared by 2-D gel electrophoresis during the late exponential growth phase. The relative abundance of some minor protein spots varied among the four strains of Bacillus. Over 123 spots of extracellular proteins were visualized on the gel for B. subtilis CH 97, 68 spots for B. subtilis 3-5, 230 spots for B. amyloliquefaciens CH 51, and 60 spotsfor B. amyloliquefaciens 86-1. 2D gel electrophoresis images of the four Bacillus strains showed significantly different protein profiles. Consistent with the 2D gel electrophoretic analysis, most of the B. subtilis proteins differed from the proteases secreted by the B. amyloliquefaciensstrains. Among the proteins identified from B. subtilis, approximately 50% were cytoplasmic and 30% were canonically extracellular proteins. The secreted protein profiles for B. subtilis CH 97 and B. subtilis 3-5 were quite different, as were the profiles for B. amyloliquefaciens CH 51 and 86-1. The four proteomes also differed in the major protein composition. The B. subtilis CH 97 and B. amyloliquefaciens CH 51 proteomes both contained large amounts of secreted hydrolytic enzymes. Among the four strains, B. subtilis 3-5 secreted the least number of proteins. Therefore, even closely related bacteria in terms of genomic sequences can still have significant differences in their physiology and proteome layout.

• Journal of Labour Economics
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• v.29 no.2
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• pp.67-116
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• 2006

This study directly analyzes the wage distributions rather than indirectly looking at a few of their moments. It also investigates wage distributions using various descriptive and semi-parametric methods. The wage distributions of Korean manufacturing industries can in general be represented by three distinct forms, underdeveloped, advanced and the medium of the two. The discrepancies in these distribution forms are explained by differences in the labor-type distributions and their weights in the composition of wage distribution forms, and further clarified through various descriptive statistics based on them. However, the descriptive statistical analysis has a limit in that it shows mixed outcomes of different categoric variables. Then, this problem is resolved by applying a semi-parametric estimation of hazard function and the marginal effect evaluations of variable changes on estimated distributions not on the function. As a result of this marginal analysis, the common features and differences of categoric variables and their intensities of effects on distributions are revealed.

• Proceedings of the KOSOMBE Conference
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• v.1998 no.11
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• pp.231-232
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• 1998

The purpose of this study was to examine whether the precipitation of calcium phosphate on titanium surface was affected by surface modification. To improve the bone conductivity, of titanium, samples were devided into 4 groups. Group 1 was immersed in 5M-NaOH solution at $60^{\circ}C$ for 24 hours. Group 2 was immersed in 5M-NaOH solution at $60^{\circ}C $ for 24 hours and heat-treated at $600^{\circ}C$ for 1 hour. Group 3 was anodized in Hanks' solution at 1V, $25^{\circ}C$ for 1 hour. Group 4 was anodized in Hanks' solution at 5V, $80^{\circ}C$ for 5 minutes. And then, all specimens were immersed in the MEM Eagle's medium whose composition was similar to that of extracellular fluid for 30 days. The precipitation of the calcium phosphate on implant surface was increased by the immersion in the NaOH solution, and more highly accelerated by heat treatment at $600^{\circ}C$. The precipitation of the calcium phosphate on titanium implant was increased with the treatment of the anodic oxidation in Hanks' solution at 5V, $80^{\circ}C$.