• Microbiology and Biotechnology Letters
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• v.20 no.1
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• pp.14-19
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• 1992

The xylanase producing microorganisms occurring on rotten woods were selectively isolated on the modified Czapek-Dox medium supplemented with 0.5% xylan as a sole carbon source. Among more than three-hundred isolates of xylanase producing microorganisms, only two bacterial isolates were turned out to be more potent xylanase producer than the reference strain of xylanase producer, Aureobaszdium pullulans NRRL Y-2311. The exo-xylanase producer, bacterial isolate No. 33 was identified as a strain of Pseudomonas sp. on the basis of morphological and biochemical characterizations as well as cellular fatty acid composition. Optima of pH and of temperature for enzyme reactions of xylanase were 5.5 and $50^{\circ}C$ respectively. The enzyme was stable in a range of pH 5.0~7.0 and below $45^{\circ}C$. Among the number of carbohydrate substrates, xylose was turned out to be a potent inducer of Pseudomonas sp. No.33 exo-xylanase. Among the raw materials tested, rice straw was the best material for xylanase production by Pseudomonas sp. strain No. 33.

• Journal of Advanced Navigation Technology
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• v.11 no.1
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• pp.1-8
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• 2007

Loran-C of ground transmitting station base that can prevent confusion of country navigation system and give BACK-UP function about electric wave navigation comparing utilization incapability state about GPS(Global Positioning System) infra that user is spreading rapidly over our society whole such as sea/aviation safety, vehicles navigation, minuteness agriculture, minuteness measurement in this treatise practical use of Loran-C navigation propose. Executed ASF(Additional Secondary Phase Factor) production and an application experiment Loran-C by location error improvement way to enhance practical use value. By the result Loran-C in conclusion that can improve location error 100~400m remarkably by 10~65m reach. Also, production extent is latitude when go composition medium and bends cotton at ASF revision table utilization of land area, this smell is judged to be suitable hardness 10 minutes. And notable location error improvement and numeric of GPS BACK-UP function are judged to be possible at a ASF revision table application to Korea Peninsula whole area hereafter.

• Nutrition Research and Practice
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• v.10 no.5
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• pp.524-529
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• 2016

BACKGROUND/OBJECTIVES: Use of smaller plates to control food intake is a commonly recommended strategy for restricting energy intake, despite conflicting results. Therefore, the aim of this study was to examine whether or not three different sizes of plates influence energy intake during a multi-itemed buffet meal in normal weight women. SUBJECTS/METHODS: This was a cross-over study conducted on 37 female participants aged 19-25 years with normal BMI levels. Participants were recruited from Hacettepe University and the surrounding community. On experimental days, participants ate a standard breakfast and were then randomly assigned to eat lunch using a small (19 cm), medium (23 cm), or large (28 cm) diameter plate. Visual analogue scale (VAS) scores on sensory and satiety outcomes were measured for all meals. Energy and macronutrient intakes during lunch were recorded. RESULTS: There was no evidence that use of a smaller plate size reduced energy or specific macronutrient intake during the free choice lunch meal. Multiple visits to the serving table were not associated with energy or macronutrient intake. Plate size did not affect VAS scores during the test days. CONCLUSIONS: Plate size did not influence energy intake, meal composition, or palatability in normal weight women during a multi-itemed open buffet lunch. Studies in natural settings at the population level are needed to clarify current outcomes.

• Journal of Plant Biotechnology
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• v.45 no.2
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• pp.146-153
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• 2018

Lilium ledebourii Bioss is a wild species of Lilium, which grows naturally in some provinces of Iran. Previous studies on Lilium tissue culture have been linked to direct regeneration and a few studies have been conducted on indirect regeneration, which has been studied under bright conditions. In this study, for the first time in the world, all the stages of indirect regeneration (callus induction, shoot and root induction) have been studied under dark conditions. Callus formation and the regeneration levels of L. Ledebourii Bioss were examined for three replicates in an MS (Murashige and Skoog) medium with different hormonal compositions and by using a factorial experiment in the framework of a completely random plan. For callus initiation, 2,4-D and kinetin hormones were used in five and four levels, respectively, as auxin and cytokinin. Results showed that the highest percentage of the callus was found in $3{\mu}M$ of 2,4-D and $0.5{\mu}M$ of kinetin. In terms of callus wet weight, the highest amount was found in $3{\mu}M$ of 2,4-D and $0.5{\mu}M$ of kinetin. In addition, in terms of diameter, the highest amount was found in $3{\mu}M$ of 2,4-D, and $0.5{\mu}M$ of kinetin. In summary, the 2,4-D hormone had a major impact on the percentage of regeneration increase so that the best response was related to the composition of $3{\mu}M$ of 2,4-D, and $0.1{\mu}M$ of kinetin. This study contended that auxin and cytokinin can induce long shoots and roots through cell elongation in dark condition.

• International Journal of Industrial Entomology and Biomaterials
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• v.25 no.2
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• pp.195-203
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• 2012

In reared populations of Allomyrina dichotoma, commercial insects, the skin of last instar larvae was changed softer with opaque white, and infested grubs eventually died. To clarify the cause of the symptom, we collected the larvae of A. dichotoma from five farms and examined their intestinal bacterial florae using pyrosequencing technique. From those results, a member of Paenibacillus was found only in the larvae showing the symptom of disease. Through PCR analysis using a Paenibacillus specific primer set, we obtained the partial 16S rRNA gene sequence and confirmed the microbe as Paenibacillus sp. For clear identification, a whole guts was extracted from each larva showing the sign of the disease and incubated at $70^{\circ}C$ for 15 min to isolate spore forming bacteria. After then, each content of guts was cultured on $MYPGP_{NAL}$ agar medium($12.5{\mu}g/ml$ of nalidixic acid) at $30^{\circ}C$. The 16S rRNA gene sequence analysis for the isolated bacteria showed that they were closely related to P. rigui(97.9% similarity), to P. chinjuensis(96.1% similarity), and to P. soli(95.3% similarity). Additional tests including API test and cellular fatty acid composition analysis were performed, but the strain couldn't be identified at species level, suggesting it may represent novel species of the genus Paenibacillus.

• Journal of the Korean Vacuum Society
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• v.8 no.3B
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• pp.290-296
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• 1999

We have studied the phase separation and ordering phenomeon of InAlAs epilayers grown on InP substrate by LP-MOCVD with DCXRD, PL, and TEM. From the intensity and FWHM of DCXRD and PL, we observed that the structural and optical quality of InAlAs epilayers were improved as growth temperature increased. The band-gap reduction due to phase separation and ordering is 291, 246, and 28 meV in the InAlAs epilayers grown at $565^{\circ}C$, $615^{\circ}C$, and $700^{\circ}C$, respectively, and shows the same from the InAlAs epilayer town at 5$65^{\circ}C$ in which the HRTEM micrograph showed the lattice fringe between InAs-rich and AlAs-rich regions was tilted by $2^{\circ}$ due to composition difference. However the maximum degree of ordering by intensity of extra spots was obtained at medium growth temperature. The annealing experiment by RTA of sample grown at $565^{\circ}$ shows a maximum band-gap shift of 78eV at $880^{\circ}$ for 3 min, and TEM shows that the origin of the blue shift of band-gap is the complete disappearance of ordering. Through annealing we can conclude that short time annealing affects only ordering and that most of the total band-gap reduction (~3/4) occurs by phase separation.

• Economic and Environmental Geology
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• v.33 no.2
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• pp.147-163
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• 2000

The Buseok pluton in the Yeongju Batholith is a comagmatic plutonic rocks which haveconcentrically compositional zoning. The lithofacies of the Buseok pluton comprise hornblende biotite tonalite in the southern part of the pluton, porphyritic and equigranular biotite granodiorite in the northern part and biotite granite in the north-central part. The compositional variations change gradually with continuity both within and between the lithofacies. The concentrically zoned pattern is relatively mafic rocks composed of high-temperature mineral assemblages in margin of the southern part, passing inward and northward gradually to more felsic rock in core of the north-central part. Changes in the textures and microstructures, as well as in the mineral content, take place between rock types of the plutons. Darker colored, generally coarse-grained, well foliated tonalite pass inward to light colored, coarse-grained, poorly foliated granodiorite, and finally give way to lighter colored, medium-grained, nearly nonfoliated granite. The foliation are best developed in the marginal part of the tonalite. Here, the regional myolitic foliation in the tonalite is steep northward and parallels to its southeastern contact with the country rock, but the magmatic foliation from disc-shaped mafic microgranitoid enclaves is subvertical and parallels the contacts with the country rock. As the tonalite approaches biotite granite in composition, the foliation is indistinct. Modal and chemical data for the pluton show quantitative compositional variation from the margin of the southern part to the core of the north-central part. Quartz and K-feldspar increase toward the core of the pluton, whereas hornblende, biotite and color index decrease. /Abundances of $SiO_2$and $K_2O$$_2$O increase toward the core according to the variation in quartz and K-feldspar, whereas those of MnO, CaO, $TiO_2$, $Fe_2O_3$, MgO and $P_2O_5$ decrease corresponding to the variation in mafic and accessaries. The compositional zonation resulted from fractional crystallization involving downward settling of earlier crystals, accompanied by upward movement of melt and volatiles, and followed by accessary marginal accretion of crystalline material in the magma to the marginal part. Although a little crustal contamination by the wall rock is recognized from the isotope data, the contamination is not only dominated over but also appropriate for forming the compositional variation in the pluton.

• Economic and Environmental Geology
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• v.16 no.2
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• pp.65-78
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• 1983

This report deals with the sedimentological study of the littoral sand of beaches in the Busan area. The purpose of this report is to know the grain size, mineralogical composition, heavy mineral and clay mineral of the beach sands, and gravity measurements of the Nagdong River Deltas. 1) As a whole, the littoral sand of the beaches are composed of uniformly medium grained, moderately sorted and nearly symmetrical. The barrier sand of the Nagdong Estuary is composed of fine grained, well sorted and nearly symmetrical. 2) The littoral sand of the beaches is transported by saltation and rolling. The barrier sand of the Nagdong Estuary is transported by suspension and saltation. 3) In the littoral sand of the beaches, the ratio of feldspar to quartz is 1 :2.31 and in the barrier sand of the Nagdong Estuary 1:1.40. 4) The content of heavy mineral of samples ranges from 0.54 to 3.87 %. The principal heavy minerals are hornblende, pyroxene, epidote, garnet, leucoxene, zircon, apatite, magnetite, hematite and ilmenite with minor accessories of rutile and olivine. 5) The x-ray diffraction analysis of the clay mineral informs the existence of quartz, feldspar, kaolinite and montmorillonite. The montmorillonite is considered to have been derived from the alteration of acidic volcanic rocks. 6) To determine the depositional structure of the Nagdong Estuary, Gravity measurements were made. Free air anomaly ranges from 14.5 mgal to 33.5 mgal and Bouguer anomaly ranges from 14.3 to 23.5 mgal and both are closely related to the topography. According to the interpreted layer structure, the upper layer composing sand, silt and clay, the intermediate layer composing sand with gravel, the lower layer composing weathered and soft rock, and bed rock composing hornfels or andesite. 7) The depositional environments of the study, the littoral area is dominated by the marine environment and the Nagdong Estuary by the mixed environment.

• Microbiology and Biotechnology Letters
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• v.17 no.5
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• pp.440-446
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• 1989

Penicillium sp. CB-20 was selected for its strong polygalacturonase activity among various strains of molds found in soil. It was found that the production of polygalacturonase reached to maximum when on the wheat bran medium containing pectin as carbon source, the strain was cultured for 60 hours at 3$0^{\circ}C$. The enzyme was purified to 29.21 food by ammonium sulfate treatment, Sephadex G-25, G-15, G-150 gel filtration, DEAE-cellulose and DEAE-Sephadex A-50 ion-exchange chromatography. Yield of the enzyme purification was 2.31 %. When the purified enzyme was applied to sodium dodecyl sulfate polyacrylamide gel electrophoresis, the molecular weight was estimated 21, 000. The amino acid composition indicated relatively high contents of gultamic acid, glycine and histidine.

• Journal of Microbiology and Biotechnology
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• v.14 no.5
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• pp.944-951
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• 2004

The influences of inoculum size, pH, and medium composition on mycelial growth and exopolysaccharides (EPS) production were investigated in shake flasks and in a bioreactor. The optimum inoculum size for both mycelial growth and EPS production was identified to be 10% (v/v) in shake flask cultures. The optimal initial pH for mycelial growth and EPS production in shake flask cultures were found to be 5.0 and 7.0, respectively. However, the optimal pH was 5.0 for both mycelial growth and EPS production in bioreactor cultures where the pH was regulated. The optimal mass ratio of the two major carbon sources, glucose to dextrin, was 1:4. The optimal mass ratio of the two major nitrogen sources, yeast extract to soy tone peptone, was 2:1. When 500 mg $1^{-1}$ of $MnSO_4-5H_2O$ was added to the bioreactor culture, both mycelial growth and EPS production were enhanced by approximately 10%. Under the optimized conditions, a mycelial biomass of 9.85 g $1^{-1}$ and an EPS concentration of 4.92 g $1^{-1}$ were obtained in 4 days.