• Proceedings of the Plant Resources Society of Korea Conference
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• 2005.11a
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• pp.179-179
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• 2005

• Journal of Ginseng Research
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• v.43 no.4
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• pp.618-624
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• 2019

Background: Ginsenoside Re (Re) is one of the major components of Panax ginseng Meyer. Ginsenoside $Rk_3$ ($Rk_3$) is a secondary metabolite of Re. The aim of this study was to investigate and compare the effects and underlying mechanisms of Re and $Rk_3$ on cyclophosphamide-induced myelosuppression. Methods: The mice myelosuppression model was established by intraperitoneal (i.p.) injection of cyclophosphamide. Peripheral blood cells, bone marrow nucleated cells, and colony yield of hematopoietic progenitor cells in vitro were counted. The levels of erythropoietin, thrombopoietin, and granulocyte macrophage colony-stimulating factor in plasma were measured by enzyme-linked immunosorbent assay. Bone marrow cell cycle was performed by flow cytometry. The expression of apoptotic protein bcl-2, bax, and caspase-3 was detected by Western blotting. Results: Both Re and $Rk_3$ could improve peripheral blood cells, bone marrow nucleated cell counts, thymus index, and spleen index. Furthermore, they could enhance the yield of colonies cultured in vitro and make the levels of granulocyte macrophage colony-stimulating factor, erythropoietin, and thrombopoietin normal, reduce the ratio of $G_0/G_1$ phase cells, and increase the proliferation index. Finally, Re and $Rk_3$ could upregulate the expression of bcl-2, whereas they could downregulate the expression of bax and caspase-3. Conclusion: Re and $Rk_3$ could improve the hematopoietic function of myelosuppressed mice. The effect of $Rk_3$ was superior to that of Re at any dose. Regulating the levels of cytokines, promoting cells enter the normal cell cycle, regulating the balance of bcl-2/bax, and inhibiting the expression of caspase-3 may be the effects of Re and $Rk_3$ on myelosuppression.

• Proceedings of the Korean Society of Crop Science Conference
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• 2006.04a
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• pp.574-575
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• 2006

• Proceedings of the Plant Resources Society of Korea Conference
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• 2005.11a
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• pp.182-182
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• 2005

• Proceedings of the Plant Resources Society of Korea Conference
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• 2005.11a
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• pp.67-67
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• 2005

• Proceedings of the Plant Resources Society of Korea Conference
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• 2006.05a
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• pp.17-17
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• 2006

• Proceedings of the Plant Resources Society of Korea Conference
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• v.18 no.1
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• pp.147-147
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• 2005

• Proceedings of the Plant Resources Society of Korea Conference
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• 2005.11a
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• pp.181-181
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• 2005

• The Journal of Korean Medicine
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• v.32 no.6
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• pp.85-94
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• 2011

Objectives: This pilot study was carried out with the purpose of suggesting a methodology on mapping and registering Korean medicinal material concepts at 2011AB of the UMLS. Methods: 411 medicinal material concepts were mapped to biomedical terminology within the metathesaurus of the UMLS. Based on the forms of listing on the UMLS and thesauri information, the medicinal material concepts were classified into three groups and mapped. Results: 76 concepts in Group 1 which English CUIs have Chinese AUIs were mapped considering scientific names and Chinese strings. 259 concepts in Group 2 that have CUIs in the form of 'botanical name/Chinese pinyin' were mapped with the information of Korean and Chinese Pharmacopoeias. Groups 3, 76 concepts of English names in the Korean Pharmacopoeia are matched considering their botanic names and used parts. Conclusion: This study suggested a methodology to map Korean medicinal material concepts to international standard terminology, which will help ensure interoperability and compatibility between traditional medicine terminology and the UMLS.

• Plant Biotechnology Reports
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• v.4 no.3
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• pp.223-228
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• 2010

Schizonepeta tenuifolia (Korean name "Hyung- Gae") is an oriental medicinal plant that is widely used in Korea, China and Japan. S. tenuifolia (Hyung-Gae) has many pharmacological activities and is mostly used for many medicinal preparations. The dried aerial part (spikes and stems) of three oriental medicinal plants, S. tenuifolia (Hyung-Gae), Agastache rugosa (Kwhak-Hyang) and Elsholtzia ciliata (Hyang-Yoo) belonging to the same family, mint family Labiaceae, have such similar shape and smell that it is difficult to differentiate between them. The trnL-F regions of chloroplast DNA of the three medicinal plants were sequenced and used as targets in multiplex PCR reaction to identify S. tenuifolia. After alignment of trnL-F sequences of the authenticated plant samples, one single nucleotide polymorphism (SNP) specific to S. tenuifolia was found. Based on this SNP, a new primer was designed that specifically amplifies the trnL-F region of S. tenuifolia. The established multiplex-PCR was proven to be effective in the differentiation of commercial S. tenuifolia samples from A. rugosa and E. ciliata. This rapid and accurate molecular method is highly promising for use in the food industry.