• Title/Summary/Keyword: medicinal fungi

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The First Report of Fusarium solani Causing Wilting in Cnidium officinale in Korea

  • Han, Kyeungmin;Lee, Hyoshin;Park, Yun Mi;Jeon, Kwon Seok;Choen, Wonsu;Jung, Chung Ryul
    • Research in Plant Disease
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    • v.27 no.2
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    • pp.76-78
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    • 2021
  • Cnidium officinale is a perennial herb belonging to the family Umbelliferae. Its origin is China and is also distributed in Korea and Japan. In 2017, a phenomenon of browning and wilting of leaves was discovered in the cultivation field of the National Institute of Forest Science, Suwon, Korea. The pathogens isolated from plants were consistent with Fusarium solani as a result of morphological examination and molecular phylogenetic analysis. This is the first record of F. solani on C. officinale in Korea.

Fatty acid composition and antioxidant capacity of some medicinal mushrooms in Turkey

  • Turkekul, Ibrahim;Cetin, Fatma;Elmastas, Mahfuz
    • Journal of Applied Biological Chemistry
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    • v.60 no.1
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    • pp.35-39
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    • 2017
  • Turkey has a very rich fungal flora due to its phytogeographical position. The screening of chemical content and active substances of mushrooms becomes an important subject not only for Turkey but also for all over the world. In the last decade, Analyses on phytochemical and biological activity of fungi have gradually increased as a result of improvement in the number and quality of facilities. In the scope of the present research, four medicinal mushrooms; Morchella elata, Lactarius volemus, Cantharellus cibarius and Tricholoma terreum were analyzed for their fatty acid compositions and antioxidant capacities. The fungal species have been found with unsaturated fatty acid/saturated fatty acid ratio of 6.73 for Morchella elata, 4.12 for Lactarius volemus, 5.21 for Cantharellus cibarius, 3.73 for Tricholama terrum. In addition, the concentration of malondialdehyde which was an indicator of lipid peroxidation was also determined in these species. According to the results, free radical scavenging activity of Morchella elata and Lactarius volemus were found higher than the other species. Any of the mushroom species investigated were found having very high metal chelating activity. The results showed that the extract of Morchella elata and Lactarius volemus exhibited significant antioxidant activities. Hence, the mushrooms have a potential to be a natural antioxidant in food industries as antioxidant agent.

Isolation of Antagonistic Bacteria against Major Diseases in Panax ginseng C.A. Meyer (인삼 주요병에 대한 길항미생물 선발)

  • Chung, Ki-Chae;Kim, Chang-Bae;Kim, Dong-Ki;Kim, Bok-Jin
    • Korean Journal of Medicinal Crop Science
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    • v.14 no.4
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    • pp.202-205
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    • 2006
  • Ginseng is major medicinal plant in Korea. Because of its long cultivation period the yield losses of 5 years of ginseng is 50% due to various diseases. The objective of this study is to select potential biocontrol agents. As the result of research so far achieved to contribute to rational prevention of ginseag plant disease for the stable cultivation of ginseng, three bacterial strains, Streptomyces lauretii strain B8180, Bacillus subtilis strain 8856, and Burkholderia cepacia strain 7944 were isolated from oak leaf compost. The strains showed antagonistic activities against five ginseng pathogenic fungi (Cylindrocarpon destructans, Rhizoctonia solani, Phytophthora cactorum, Botrytis cinerea, Fusarium solani f. sp. panacis) and control effects on Phytophthora blight.

Diversity and Bioactive Potential of Culturable Fungal Endophytes of Medicinal Shrub Berberis aristata DC.: A First Report

  • Sharma, Supriya;Gupta, Suruchi;Dhar, Manoj K.;Kaul, Sanjana
    • Mycobiology
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    • v.46 no.4
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    • pp.370-381
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    • 2018
  • Bioactive natural compounds, isolated from fungal endophytes, play a promising role in the search for novel drugs. They are an inspiring source for researchers due to their enormous structural diversity and complexity. During the present study fungal endophytes were isolated from a well-known medicinal shrub, Berberis aristata DC. and were explored for their antagonistic and antioxidant potential. B. aristata, an important medicinal shrub with remarkable pharmacological properties, is native to Northern Himalayan region. A total of 131 endophytic fungal isolates belonging to eighteen species and nine genera were obtained from three hundred and thirty surface sterilized segments of different tissues of B. aristata. The isolated fungi were classified on the basis of morphological and molecular analysis. Diversity and species richness was found to be higher in leaf tissues as compared to root and stem. Antibacterial activity demonstrated that the crude ethyl acetate extract of 80% isolates exhibited significant results against one or more bacterial pathogens. Ethyl acetate extract of Alternaria macrospora was found to have potential antibacterial activity. Significant antioxidant activity was also found in crude ethyl acetate extracts of Alternaria alternata and Aspergillus flavus. Similarly, antagonistic activity of the fungal endophytes revealed that all antagonists possessed inhibition potential against more than one fungal pathogen. This study is an important step towards tapping endophytic fungal diversity for bioactive metabolites which could be a step forward towards development of novel therapeutic agents.

Characteristic for Growth and Fruit Body Polysaccharide of Caterpillar Fungi, Cordyceps sobolifera(Hill.) Berk. et Br.

  • Ohga, Shoji;Yoshimoto, Hiroaki;Pokhrel, Chandra;Yang, Bai-Song;Miyazawa, Noriko;Meng, Tianxiao;Hosoda, Shun;Mae, Masuo;Sun, Zhu
    • Korean Journal of Agricultural Science
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    • v.35 no.2
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    • pp.199-212
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    • 2008
  • Mycelial expansion and fruit body development of caterpillar fungi, Cordyceps sobolifera(Hill.) Berk. et Br. was examined on various carbon and nitrogen sources, some substrates with various supplements. Best temperature for the mycelial expansion was $25^{\circ}C$, while optimal pH was 7. Glucose and polypeptone were the best sources of carbon and nitrogen, respectively, as well as 1:1 was the best ratio of carbon and nitrogen. The unpolish rice and bean curd lees were used as core substrates combined individually with five various supplements. In substrates based, all the supplements with all combinations were found to exhibit the mycelial growth and fruit body formation. Likewise, yolk was found as a superior supplement and also clarified quite high polysaccharide content in the fruit body.

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Screening of Antifungal Medicinal Plants for Turfgrass Fungal Disease Control (잔디 병해 방제를 위한 항균성 약용식물의 탐색)

  • Kwon, Soo-Mean;Kim, Dae-Ho;Chang, Tae-Hyun;Jeon, Min-Goo;Kim, In-Seob;Kim, Ik-Hwi
    • Asian Journal of Turfgrass Science
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    • v.24 no.2
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    • pp.176-181
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    • 2010
  • Brown patch (Rhizoctonia solani AG1-1), dollar spot (Sclerotinia homoeocarpa), pythium blight (Pythium spp.), anthracnose (Colletotrichum graminicola), yellow patch (Rhizoctonia cerealis) and Zoysia patch (Rhizoctonia solani AG2-2) are the major turfgrasses diseases in Korea. In this study, 23 medicinal plant extracts were tested for antifungal activities against turfgrass pathogenic fungi. In paper disk diffusion method, 12 medicinal plant extracts, including Sophora flavescens, showed antifungal activity. Also, in the test of antifungal activity on media contained the extracts of S. flavescens, Curcuma longa, Rheum undulatum, Coptis chinensis and Asiasarum sieboldi showed above 80% inhibitory effects on the mycelial growth in 110 mg/10 ml concentration of the extracts. S. flavescens, in particularly, showed antifungal activity against the six turfgrass pathogenic fungi. The inhibition rate of S. homoeocarpa was 100% in 10 mg/10 ml, 5 mg/10 ml and 2 mg/10 ml concentrations of C. longa extract. In case of Pythium spp., the extracts of S. flavescens, R. undulatum and C. chinensis showed 100 % inhibition rate on the test media.

Occurrence of the Phytophthora Blight Caused by Phytophthora sansomeana in Atractylodes macrocephala Koidz. (Phytophthora sansomeana에 의한 큰꽃삽주 역병 발생 보고)

  • An, Tae Jin;Park, Myung Soo;Jeong, Jin Tae;Kim, Young Guk;Kim, Yong Il;Lee, Eun Song;Chang, Jae Ki
    • Korean Journal of Medicinal Crop Science
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    • v.27 no.6
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    • pp.404-411
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    • 2019
  • Background: In September 2017, wilting and rhizome rot symptoms were observed on Atractylodes macrocephala Koidz. in Jecheon-si and Eumseong-gun. This study was carried out to isolate hitherto unidentified pathogenic fungi from A. macrocephala and to test the pathogenicity of isolated fungi against Atractylodes spp. genus such as A. macrocephala, A. japonica, and their interspecific hybrids. Methods and Results: The diseased plants were washed with running tap water, and the boundary between the healthy area and the diseased area was cut while the pathogens were isolated by growing cultures from the diseased areas on Phytophthora semi-selective medium. The internal transcribed spacer (ITS) region of the isolates was used in this study for identification. Test plants were cultivated in the glasshouse at 20℃ - 30℃ for 4 months and then used for pathogenicity test. The pots with plants inoculated with mycelial plugs and zoospores were placed at 25℃ for 48 h in a dew chamber where relative humidity was above 95%, and then moved into the glasshouse at 20℃ - 30℃. The presence or absence of pathogenicity of the strains was determined by evaluating the symptom of plant wilting. The inoculation test was performed in three replicates with a non-treated control. Conclusions: On the basis of results of ITS sequence analysis, the strains isolated from the diseased plants was identified as Phytophthora sansomeana. Biological assay using test plants confirmed the pathogenicity of P. sansomeana against Atractylodes macrocephala. This is the first report of rhizome rot in A. macrocephala caused by P. sansomeana.