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The most appropriate antimitotic treatment of Ara-C in Schwann cell-enriched culture from dorsal root ganglia of new born rat

  • Kim, Soung-Min;Jahng, Jeong-Won;Lee, Jong-Ho
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • v.32 no.1
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    • pp.42-51
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    • 2006
  • Schwann cell, one of important components of peripheral nervous system, interact with neurons to mutually support the growth and replication of embryonal nerves and to maintain the different functions of adult nerves. The Ara-C, known as an antimitotic agent, have been used to have high effectiveness in eliminating fibroblasts during Schwann cell culture period. This enrichment effect is also known to be cummulative with each successive pulse of Ara-C applied and is due to a progressive loss of fibroblasts. But the cytotoxicity by Ara-C is also cummulative and noticeable over the period. To determine the most effective application time and interval of Ara-C in the Schwann cell culture, we observed the Schwann cell purity and density with the Ara-C treatment in plain and three-dimensional culture from dorsal root ganglion of new born rat. By culturing dispersed dorsal root ganglia, we can repeatedly generate homogenous Schwann cells, and cellular morphology and cell count with mean percentages were evaluated in the plain culture dishes and in the immunostainings of S-100 and GFAP in the three-dimensional culture. The Ara-C treated cultures showed a higher Schwann cell percentage (31.0%${\pm}$8.09% in P4 group to 65.5%${\pm}$24.08% in P2 group), compared with that obtained in the abscence of Ara-C (17.6%${\pm}$6.03%) in the plain culture after 2 weeks. And in the three-dimensional culture, S-100 positive cells increased to 56.22%${\pm}$0.67% and GFAP positive cells to 66.46%${\pm}$1.83% in G2 group (p<0.05), higher yield than other groups with Ara-C application. Therefore, we concluded that the Ara-C treatment is effective for the proliferation of Schwann cells contrast to the fibroblasts in vitro culture, and the first application after 24 hours from cell harvesting and subsequent 2 pulse treatment (P2 group in plain culture and G2 group in three-dimensional culture) was more effective than other application protocols.

The Analysis of Pattern Identification of Low Back Pain, Which is Used in Thesis both in Korea and China (한국과 중국 논문에서 사용된 요통 변증에 관한 고찰)

  • Kim, Min-Woo;Ko, Youn-Seok;Lee, Jung-Han;Chung, Won-Suk;Shin, Byung-Cheul;Cha, Yun-Yeop;Go, Ho-Yeon;Sun, Seong-Ho;Jeon, Chan-Yong;Jang, Bo-Hyoung;Song, Yun-Kyung;Ko, Seong-Gyu
    • Journal of Korean Medicine Rehabilitation
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    • v.23 no.2
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    • pp.85-94
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    • 2013
  • Objectives : This study aims to contribute to developing new pattern identification based on searches regarding pattern identification of low back pain, which is used in thesis both in Korea and China. Methods : First of all, we searched thesis concerning pattern identification of low back pain from RISS, OASIS, Korean traditional knowledge portal, CNKI. Results : 1. There were overall 34 thesis, consist of 18 Korean thesis(13 clinical papers and 5 analytical papers) and 9 Chinese thesis(7 clinical papers and 9 analytical papers). 2. 10 of 11 Korean thesis used more than 9 patterns for pattern identification, 9 of 14 Chinese thesis used less than 4 patterns for pattern identification of low back pain. 3. Patterns, which were repeatedly used in Korea, were 腎虛腰痛(Kidney deficiency low back pain), 濕熱腰痛(Dampness-heat low back pain), 寒濕腰痛(Cold-dampness low back pain), 痰飮腰痛(Phlegm-fluid retention low back pain), 風腰痛(Wind low back pain), 食積腰痛(Food accumulation low back pain), 濕腰痛(Dampness low back pain), 挫閃腰痛(Sprain low back pain), 瘀血腰痛(Static blood low back pain), 氣腰痛(Qi low back pain). 4. Patterns, which were repeatedly used in China, were 腎虛腰痛(Kidney deficiency low back pain), 濕熱腰痛(Dampness-heat low back pain), 寒濕腰痛(Cold-dampness low back pain), 氣滯血瘀腰痛(Blood stasis due to qi stagnation low back pain). Conclusions : Based on these results, it is considered that an advanced type of pattern identification of low back pain should be made or existing type needs to be practically and objectively improved.

Strategic Approaches to Occupational Health in Korean Medicine by Reviewing Research Trends (산업보건 관련 한의계의 연구동향 및 접근방안 모색)

  • Lee, Jung-Han;Jo, Dong-Chan;Ko, Youn-Seok;Cha, Yun-Yeop;Chung, Won-Suk;Shin, Byung-Cheul;Jeon, Chan-Yong;Go, Ho-Yeon;Sun, Seong-Ho;Jang, Bo-Hyoung;Song, Yun-Kyung;Ko, Seong-Gyu
    • Journal of Korean Medicine Rehabilitation
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    • v.23 no.2
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    • pp.105-115
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    • 2013
  • Objectives : This study was aimed to review the trend of occupational health in korean medicine and to suggest the strategic approach. Methods : We collected 40 articles on occupational health in the internet site OASIS using the keyword 'workplace', 'worker'. Then we analyzed them. Results : There were 40 papers that were published between 2000 and 2012. Published papers on the korean journal of oriental preventive medicine were more than the other journals. Affiliation of the first author of the published papers was concentrated to one. Clinical research papers accounted for 90% of the total study. Papers on health management were more than half. Conclusions : Related to occupational health, high quality and large amount of research and development is needed and the active policy of the government should be supported in Korean medicine.

Research Trends of Work-Related Musculoskeletal Disorders in Korean Literature (작업관련성 근골격계 질환의 국내 연구동향분석)

  • Ko, Youn-Seok;Park, Hun-Sang;Lee, Jung-Han;Cha, Yun-Yeop;Chung, Won-Suk;Shin, Byung-Cheul;Jeon, Chan-Yong;Go, Ho-Yeon;Sun, Seong-Ho;Jang, Bo-Hyoung;Song, Yun-Kyung;Ko, Seong-Gyu
    • Journal of Korean Medicine Rehabilitation
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    • v.23 no.2
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    • pp.95-104
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    • 2013
  • Objectives : The purpose of this study is to analyze internal research trends of work-related musculoskeletal disorders(WMSDs) and provide problems of researches forward. Methods : 6 Korean databases were searched for articles of WMSDs published from 2000 to 2012, and 264 research were systematic reviewed. An analytical method was used descriptive statistics, an actual number and percentage. Results : The results of distribution by year were reported more than 20 articles after 2004, and 45 articles in 2009. Industrial classification distribution of research subjects the manufacturing industry was many most at the 84. Research contents in most description were 147 whether it was risk factor and relation. Research design type of articles was most description survey research 226. In the research field 89 articles were reported to the journal related to technologies. Conclusions : It is considered to be necessary that ergonomic approach would cooperate with other approaches such as integrated health management system as well as industrial medicine considering psychosocial factors.

THE EFFECT OF SCREW TIGHTENING SEQUENCE AND TIGHTENING METHOD ON THE DETORQUE VALUE IN IMPLANT-SUPPORTED SUPERSTRUCTURE (임플랜트 지지 상부구조물에서 나사조임순서와 조임방법이 풀림토크값에 미치는 영향)

  • Choi, Jung-Han;Kim, Chang-Whe;Lim, Young-Jun;Kim, Myung-Joo;Lee, Seok-Hyung
    • The Journal of Korean Academy of Prosthodontics
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    • v.45 no.5
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    • pp.653-664
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    • 2007
  • Statement of problem: The screw detorque value is a measure of the preload remaining in the screw just before detorquing. Purpose: This study evaluated the effect of different screw tightening sequences and tightening methods on detorque values for a well-fitting implant superstructure. Material and method: An implant superstructure that connected directly to four implants (Astra Tech) was fabricated on a fully edentulous mandibular acrylic resin model. Six well-fitting dental stone casts were made with a pickup impression of the superstructure from the acrylic resin model. To evaluate the effect of three screw tightening sequences (1-2-3-4, 2-4-3-1, and 2-3-1-4) and two tightening methods (2-step and 1-step) on the stability of screw joint, the detorque values for a well-fitting implant superstructure were measured twice after screw tightening using 20 Ncm. Detorque values were analyzed using multi-way analysis of variance and two-way analysis of variance at a .05 level of significance. Results: 1. The mean detorque values for three screw tightening sequences were 12.3 Ncm, 12.6 Ncm, and 12.0 Ncm, respectively. 2. The mean detorque values for two screw tightening methods were 12.0 Ncm, and 12.2 Ncm, respectively. 3. The mean of mimimum detorque values for three screw tightening sequences and for two tightening methods were 10.6 Ncm, 11.1 Ncm, 10.5 Ncm, and 9.8 Ncm, respectively. 4. No statistically significant differences among the variables of screw tightening sequence and tightening method were found (p>.05) for detorque values and for mimimum detorque values. Conclusion: Within the limitations of this study, the screw tightening sequence and tightening method did not have a significant effect on the detorque values for a well-fitting implant superstructure.

A Study on Health Care Activities of Some Industrial Nurses and their Related Factors in Kyungnam Area (경남지역 일부 산업간호사의 보건관리 업무 및 관련요인에 관한 연구)

  • Kim, Young Sook
    • Korean Journal of Occupational Health Nursing
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    • v.4
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    • pp.48-57
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    • 1995
  • The purpose of this study was to assess the performance of the role and function of some industrial nurses and to characterize the factors affecting the performance of their activities. Thus the results could be used to suggest the direction in the performance of industrials nurses' activities effectively. During a period from January 10 to March 31, 1994, the data were collected from 87 industrial nurses, who were working as health managers in the plants, in Ulsan city and the vicinity in Kyungnam province, using a structured questionnaire. The results were as follows : 1. The general characteristics of industrial nurses in this study were 82.8% being 30 years old or less, 60.9%, being not married, and 93.1% having eduction levels above junior college. 2. With respect to general work conditions, 94.3% were working in a separate room provided for health care division, 40.2% working under the safety and health department, and 98.9% working as common-level staffs. And 60.9% were working less than 44 hours a week, 70.1% had work experiences less than 5 years, and 50.6% had annual incomes ranging 10 to 14 million wons. 3. As work conditions related to health care activities, 49.4% performed the activities not related to health care as always or occasionally, and 87.4% answered that occupational physicians were appointed in their plant and among them, however, only 6.9% worked on full-time basis and 52.8% perform little activities as occupational physicians. For a decision related to health care activity, 69.0% discussed the problems with the supervisors, and 19.5% made decisions by themselves. 4. As for attitude and perception to their activities as health managers, 66.7% moderately recognized the importance of health manager in the workplace, with 63.2% being satisfied their wages and treatment from the company, 57.5% being satisfied with their job positions and 51.7% having positive attitudes as being health managers. 5. The degree of performance at least in one of health related activities were very high in activities such as general medical care(100%), general health examination(98.0%) and specific health examination(100%), and relatively high in health education(72%), new employee health examination(60.9%), document handling(79.3%) and activity for work environment(70.1%). However, the performance rate was very low in preparing protective equipment (20.8%). 6. The levels of activities related to health care were significantly high when making decisions by themselves, when occupational physicians not being full-time, and when satisfying their job positions, and, on the other hand, significantly decreased as work hours increased. 7. In addition to some kinds of periodic education asked by all of the nurses, 89.7% wanted a specialized licensing system for industrial nurse, and 97.4% wanted to apply for the license test. As a conclusion, it is suggested that industrial nurses should be given more authority and placed in more self-controlled system to perform health care and other activities more efficiently, and the role and function of the occupational physician should be clearly distinguished from that of the industrial nurse as a health manager to avoid an unnecessary overlapping.

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Role of Matrix Metalloproteinase in the Pathogenesis of Bronchial Asthma (기관지 천식의 병인에서 Matrix Metalloproteinase의 역할)

  • Lee, Yong-Chul
    • Tuberculosis and Respiratory Diseases
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    • v.53 no.2
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    • pp.101-112
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    • 2002
  • Background : Toluene diisocyanate(TDI) is a leading cause of occupational asthma. However, the pathogenesis of TDI-induced asthma is largely unknown because there is no suitable animal model. Materials and Methods : We developed a murine model of TDI-induced asthma by performing two sensitization with 3% TDI and one challenge with 1% TDI using ultrasonic nebulization. Results : Similar to occupational asthma in humans, murine TDI-induced asthma includes findings 1) increased inflammatory cells, including neutrophils and eosinophils, 2) histologic changes, including infiltration of inflammatory cells around bronchioles, thickened airway epithelium, contraction of bronchioles, and accumulation of mucus and debris in the bronchioles, 3) increased MMP-9 activity in inflammatory cells in the airway lumen, 4) airway hyperrespnosiveness. Administraion of an MMP inhibitor, MMPI-I, remarkably reduced all these pathophysiological findings. Conclusion : Therefore, we conclude that TDI-induced occupational asthma is associated with the induction of MMP-9 in inflammatory cells, and the inhibition of MMP-9 may be a good therapeutic strategy.

A Retrospective Study of Sintered Porous-surfaced Dental Implants in Restoring the Edentulous Posterior Mandible: Up to Eight Years of Functioning (하악 구치부에 식립한 sintered porous surfaced implants의 후향적 다기관 연구)

  • Kim, Woo-Sung;An, Kyung-Mi;Sohn, Dong-Seok;Jung, Heui-Seung;Shin, Im-Hee
    • The Journal of the Korean dental association
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    • v.47 no.12
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    • pp.823-829
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    • 2009
  • Purpose : The aim of this study was to evaluate the survival rate of sintered porous-surfaced implants placed in the edentulous posterior mandibles, in relation to implant length and diameter, crown-to-implant ratio, and types of prostheses, for a maximum of eight years of functioning. Material and Methods : The study group consisted of 43 partially edentulous patients who visited Catholic University Hospital of Daegu and one private dental clinic. A total of 122 sintered porous-surfaced implants n $Endopore^{(R)}$ (Inn ova Life Sciences, Toronto, Ontario, Canada) -- were placed in the edentulous posterior mandibles, Two diameter sizes (4.1 mm and 5.0 mm) and four lengths (5.0 mm, 7.0 mm, 9.0 mm, and 12.0 mm) were used. One hundred and three implants were splinted and 21 implants were nonsplinted. The survival rates of the implants in relation to length, diameter, crown-to-implant ratio, and types of prostheses were investigated. Statistical data were analyzed using SPSS Win.Ver 14.0 software with the Chi-square test. Results : The survival rate of the 4.1mm diameter implants was 100% and 91.2% for the 5.0mm diameter implants. The survival rates of the implants of differing diameters were found to be statistically different (p=0.005). The survival rates of both the 5.0mm and 7.0 mm length implants were 100%. The survival rate of the 9.0mm length implants was 97.9% and for the 12.0mm length implants was 95.1%. There was no statistical difference in survival rates for the differing lengths of implants. Of the 103 prostheses that were splinted, the survival rate was 98.0%. The survival rate of splinted prostheses was higher than that of the non-splinted prostheses, but was found to be not statistically different. There were no failed cases when the crown-to-implant ratio was under 1.0. When the crown-to-implant ratio was between 1.0 and 1.5, the failure rate of the implants was 6.7%. No failure was recorded with the ratio range of 1.5 to 2.0. Relative to the crown-to-implant ratio of 1.0, the failure rates were statistically different (p=0.048). Discussion and Conclusion : The cumulative survival rate of the porous-surfaced implants placed in the edentulous posterior mandibles was 97.5%. Short porous-surfaced implants showed satisfactory results after a maximum of nine years of functioning in the edentulous posterior mandibles.

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The Compare Physical and Psychological Functioning by Sex, Weight and Age in Judo Athletes (유도선수의 성별, 체급 및 연령에 따른 신체적 기능과 심리적 기능의 비교)

  • Kim, Hye-Young;Chung, Eun-Jung;Lee, Byoung-Hee
    • The Journal of the Korea Contents Association
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    • v.20 no.3
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    • pp.678-686
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    • 2020
  • The purpose of this study was to compare the physical and psychological functioning by sex, weight and age in Judo athletes. The 124 participants; high school students, college students, and team players. They used physical tests to measure physical examinations by self-describing Knee injury and Osteoarthritis Outcome Score test, Foot and Ankle Outcome Score test, and Oswestry Disability Index, and psychological tests such as Profile of Mood Test, Athletes' Self-Management Questionnaire, and The Athletic Coping Skills Inventory-28, to examine the effects of physical and psychological functions of sports athletes. The physical tests of sex showed differences in knee and back, psychological tests of sex showed differences in mood state, self-management, and athletic coping skills. As a result of physical examination by weight, there was a difference in knee pain and sports items, psychological tests showed that there was a significant difference in mood state except for vitality. Physical examination by age showed differences in knee, ankle, and back. As a result of age-based psychological test, mood state test showed difference in all items except tension and vitality. There was a significant difference only in the items of physical management in self-management test. The results of this study are expected to be used as a basic data for sports injury training and medical technology development by sex, weight and age of Judo athletes.

Risk Factors of Arthritis in Middle-aged Women in Korea (중년여성의 관절염 발생 영향요인 분석)

  • Yang, Kyung-Mi;Lee, Jong-Eun;Jung, Hye-Sun;Kim, Sook-In;Lee, So-Young;Lee, Yoon-Jung;Kim, Soon-Lae
    • Research in Community and Public Health Nursing
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    • v.17 no.4
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    • pp.512-520
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    • 2006
  • Purpose: To investigate the risk factors of arthritis, this study was carried out with 1,829 Korean women aged between 40-64 who responded to a health behavior survey. Methods: Data were extracted from the results of the 2001 National Survey on Health and Nutrition conducted by the Ministry of Health and Welfare. Results: 1 Financial difficulty and continuous anxiety about health were directly associated with arthritis. The odd ratios of these two factors of arthritis were 1.43 and 1.82 respectively. 2. With regard to the presence or absence of arthritis according to demographic characteristic, the incidence of arthritis was higher in the age between 50-64, in the group whose education level was middle school or lower, and in those who experienced the death of spouse, divorce or separation. Nevertheless, it was not significantly different according to financial status and employment status. 3. Concerning the presence or absence of arthritis according to psychological characteristic, the incidence of arthritis was found to be higher in those who were not healthy by subjective health assessment, in those who were always anxious about their health, in those who were always depressed and sad, and in those who felt heavy stress. 4. With regard to the presence or absence of arthritis according to the health behavior, the incidence of arthritis was higher in postmenopausal cases and cases who were having hormone therapy, and the incidence of arthritis was higher in those who did not drink alcohol, those with BMI higher than 25, and those who slept shorter than 7 hours a day. Conclusion: Based on the above results, it is required to provide a means applicable to communities by developing preventive education that can prevent the incidence of arthritis in middle-aged women as well as intervention programs.

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