• ALGAE
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• 제34권1호
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• pp.45-56
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• 2019

Ecklonia cava (EC) has been widely utilized as an ingredient in commercial products such as functional foods and cosmeceuticals. Recently it has been found that Sargassum horneri (SH) has been invading on Jeju Island coast area by its huge blooming. Moreover, both seaweeds are considering as important ingredients in traditional medicine specifically in East-Asian countries (China, Japan, and Korea). In the present study, we attempted to compare anti-inflammatory properties of 70% ethanolic extracts of EC (ECE), SH (SHE), and their different combinations on lipopolysaccharide (LPS)-activated RAW 264.7 cells. Results indicated that 8 : 2 combinations of ECE : SHE significantly inhibited LPS-activated inflammatory responses (cytokines, protein, and gene expression) in RAW 264.7 macrophage cells compared to the respective extracts and other combinations. The synergistic effect of ECE and SHE was found to be prominent than the effects of ECE or SHE alone. These observations provide useful information for the industrial formulation of functional materials (functional foods and cosmeceuticals) using these two particular seaweeds in Jeju Island of South Korea.

• Journal of Community Nutrition
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• 제1권1호
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• pp.44-51
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• 1999

The purpose of this study was to compare dietary behavior and food intake in relation to physical complaints, such as farmer' syndrome(FS) and gastro-intestinal(GI) problem among Korean farmers. The questionnaire was composed of 24 Cornell Medical Index (CMI), 8 farmers' syndrome, and 5 GI problem questions. Food intake data was gathered by the semi-quantitative food frequency method. The subjects(male 226, female 415) who had FS and Gi problem were 12./8% and 8.3%, respectively. The physical complaints were higher in female and the elderly group. In the FS group, lower activity was seen that in normal groups. The lower health status and fatigues were found in the physical complaint groups. Dietary behavior showed low appetite in the physical complaint group, irregular lunch in FS, and irregular breakfast in GI problem group. Changes in dietary behavior were shown in the aspects of lower amount of intake quantity(40.8%), lower consumption in fat(32.8%) and salty(38.8%) foods, and diverse food items(47.8%). Quantity of food consumed was significantly different withing groups with FS. Kinds of food consumed, intake of protein source foods, milk and calcium and total animal foods were lower in the FS group. But milk and calcium source food and all animal food intakes were higher in the GID problem group. The results suggest that dietary behavior and food intake differ within the group of physical complaints.

• 동아시아식생활학회지
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• 제13권6호
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• pp.552-560
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• 2003

In this study, Artemisia capillaris, which has been used in oriental medicine and folks remedy, was investigated to characterize the nutritional composition (protein, lipid, ash, fiber, amino acids, fatty acids, Na, K, P, Mg, Ca and Fe) and effective substance (scopoletin). Artemisia capillaris has often been cited in medical literature for its medicinal effects. The Korean Food and Drug Administration allowed Artemisia capillaris as a food stuff and indicated as In-Jin-Sook. From the view point of the subjects who eat In-Jin-Sook products, most of them ingested it for a certain pharmacological efficacy rather than as a beverage or a food. Therefore, we need to develop functional foods using In-Jin-Sook. Artemisia capillaris was superior in protein, crude fiber, amino acid and fatty acid compositions to wild greens according to Korean Nutritional Composition Table. Also scopoletin, blown for blood vessel relaxation and hypotensive effect, was detected at the level of 326.13 $\mu\textrm{g}$/g. These results demonstrated that Artemisia capillaris has both pharmaceutical effects and balanced nutritional contents. Artemisia capillaris has sufficient values to use as a food stuff for functional foods.

• Journal of Nutrition and Health
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• 제31권5호
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• pp.957-964
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• 1998

The study was undertaken to assess the degree to which subjects were compliant with a specific metabolic diet in a controlled outpatient feeding study. The study consisted of a two-week control period in which subjects consumed a control diet consisting of 38-40% fat, 18-20% protein, 40-42% carbohydrate, followed by a three-week experimental period during which each subject consumed the control diet plus one of the four fiber supplements(20g/4ay) assigned on a random basis on the trust day. All meals were prepared and eaten in a metabolic feeding laboratory, with the exception of Saturday and Sunday meals that were packed for take-out. Fifty-seven healthy adult men, aged 18-65 years participated in the study. Dietary compliance index(CI) defined as the percent deviation of actual consumption from the prescribed food's and unconsumed prepared foods. The CI reflected the additional 'non-prescribed foods' and unconsumed 'prescribed foods'. A CI was calculated for all subjects. A CI of 5% or more was defined as poor compliance. Overall, dietary compliance was substantially improved for the entire group over the course of the study. Significant increase in compliance far energy and macronutrient intake occurred between the baseline and experimental periods. Within a non-obese group, dietary compliance fir energy improved from 5.5% to 3.3% by the end of the study(week 1vs week 5, respectively, p<0.05). However, between non-obese and obese group, dietary compliance was not different. (Korean J Nutrition 31(5) : 957-964, 1998)

• Asian Pacific Journal of Cancer Prevention
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• 제13권5호
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• pp.2291-2297
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• 2012

Objective: Gastric cancer is an important public health problem in the world and Turkey. In addition to Helicobacter pylori (H. pylori), smoking, alcohol consumption and family history, certain dietary factors have been associated with its occurrence. The impact of dietary habits and life-style factors on the risk of gastric cancer in Turkey were evaluated in this study. Design: A questionnaire was applied to 106 patients with gastric adenocarcinoma and 106 controls without cancer matched for age (range 28-85 years) and gender selected from a hospital based population. Adjusted odds ratios (ORs) and 95% confidence intervals (CI) were calculated with logistic regression analysis. Results: The incidence of H. pylori was 81.3% in patients. Frequent consumption of salty dishes, very salty foods like pickles, soup mixes, sausages, foods at hot temperature (ORs = 3.686, 7.784, 5.264, 3.148 and 3.273 respectively) and adding salt without tasting (OR = 4.198) were associated with increased gastric risk. Also heavy smoking and high amount of alcohol consumption (p = 0.000) were risk factors. Frequent consumption of green vegetables, onion, garlic and dried fruits (ORs = 0.569, 0.092, 0.795 and 0.041) was nonsignificantly associated with decreased risk. Conclusion: Improved dietary habits, reducing salt consumption and eradication of H. pylori infection may provide protection against gastric cancer in Turkey.

• 한국식품영양과학회지
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• 제44권1호
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• pp.14-23
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• 2015

한국인 다소비 탄수화물 간식류의 조리방법에 따른 혈당지수 및 혈당부하지수를 알아보기 위하여 건강한 성인에게 포도당과 탄수화물 간식을 조리방법을 달리하여 실험하였다. 당질부하량은 50 g 당질 함량으로 계산하였고, 식후 혈당과 혈청 인슐린 반응은 2시간에 걸쳐 측정하였다. 그 결과 옥수수죽, 찐옥수수, 찐감자, 군감자, 찐고구마, 군고구마는 고혈당지수에 해당하였고 강냉이, 고구마튀김, 군밤이 중 혈당지수에 해당되며, 감자튀김, 감자전, 군밤, 찐팥, 팥죽은 저혈당지수 수치에 해당하였다. 조리방법별로 비교했을 때 기름을 사용한 찐감자와 군감자의 혈당지수가 감자튀김과 감자전보다 높았고 군고구마의 혈당지수가 고구마튀김에 비해 높았으며, 찐팥의 혈당지수가 팥죽에 비해 낮았다. 식품별로 비교했을 때 찐 형태에서는 찐팥이 저 혈당지수, 찐밤이 중 혈당지수 간식에 해당하였고, 구운 형태로는 군밤이 저 혈당지수 식품에 속하였다. 죽 형태로는 팥죽이 저 혈당지수에 해당하였고, 튀긴 형태로는 감자전, 감자튀김 모두 저 혈당지수에 속하였지만 감자전만이 고구마튀김에 비해 낮았다. 혈당부하지수는 찐팥, 군밤, 찐밤, 팥죽, 군감자, 찐감자, 감자전, 감자튀김이 저 혈당부하지수, 고구마튀김, 강냉이, 찐옥수수가 중 혈당부하지수, 군고구마와 옥수수죽은 고 혈당부하지수 간식에 해당하였다. 결론적으로 현재 탄수화물 간식류에 대한 혈당지수는 외국의 데이터를 많이 이용하고 있고 국내에서의 연구는 미비한 실정이므로 우리나라에서 많이 섭취되고 있는 간식류를 대상으로 혈당지수 연구가 지속적으로 이루어져야 할 것으로 사료된다. 또한 기름을 사용하여 조리된 간식류는 혈당지수가 낮더라도 만성질환예방을 위해 적당량 섭취를 권고해야 할 것이다. 마지막으로 당뇨병의 식사요법에서 탄수화물 간식류는 무조건 제한하기보다는 적절한 조리방법으로 제시될 필요가 있으므로 본 연구에서 산출한 탄수화물 간식류의 혈당지수와 혈당부하 지수를 참고적인 자료로 이용할 수 있을 것으로 생각되며 향후 당뇨병 환자를 대상으로 한 탄수화물 간식류의 혈당지수 연구도 필요할 것으로 사료된다.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2000년도 추계학술대회 논문집
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• pp.64-67
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• 2000

It is usual that the medical examination of human body need special knowledpe and equipments. Thus we have to spend Lime and energy on going to special place such as hospital where doctors and equipments are. Which often cause missing a good chance af medical treatment as well as giving us inconvenience. However many simple and convenient equipments were developed for checking our health conditions recently. O-ring test is accepted a3 one of useful methods to examine our heallb conditions. Also the test is recognized as a uscful means to judge, withoul any special equipment and medical knowledge, if some medical substances or foods are beneficial or harmful to our health. However, the judgement may be mcorrect because it depend on doctor's subjective point of view.In this study, we developed an automatic O-ring t a r mach~ne which enable us to check our health conditions objectively and quantitatively. The validity of the idea to develope the machine was proved by experiments.

• 한국식품영양과학회지
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• 제38권10호
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• pp.1353-1361
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• 2009

본 연구는 서울지역에 거주하는 건강한 성인 남녀(남자 29명, 여자 10명) 39명을 대상으로 trans 지방산 3.75 g을 각각 첨가한 찹쌀보리빵과 밀빵의 섭취가 혈중 지방산 조성과 지질 수준에 미치는 효과를 확인하기 위하여 실시하였다. 시험군은 밀빵 군(19명)과 찹쌀보리빵 군(20명)의 2군으로 분류하였으며 장기(6주) 임상시험으로 시행하였다. 밀빵 군은 밀가루 80 g에 trans 지방산 3.75 g을 첨가한 밀빵을, 찹쌀 보리빵 군은 $\beta$-glucan 5%를 함유한 찹쌀보리가루 40 g과 밀가루 40 g에 trans 지방산 3.75 g 첨가하여 찹쌀보리빵을 조제하여 제공하였다. 찹쌀보리빵을 섭취한 군에서는 섭취기간이 증가할수록 C18:3c,n-9 지방산을 제외한 지방산들의 농도가 낮아졌으며 특히 섭취 6주경과 후에서는 C16:0, C18:1t, C18:1c, C18:2t와 C18:2c의 농도는 밀빵을 섭취한 군에 비해 유의적으로 낮은 수준을 보였다. 밀빵을 섭취한 군에서 섭취기간이 증가할수록 C18:1t, C18:2c, C18:2t의 농도는 증가하는 것으로 나타난 반면 C18:0, C18:1c, C18:3c,n-9과 C18:3c,n-3의 농도는 감소하였고 C16:0와 C18:2c의 농도는 증가 후 일정수준을 유지하여 6주경과 후에서는 섭취 전보다 현저하게 높은 수준을 보였다. P/M/S 비를 보면 섭취4주까지 두 군 모두에서 다가불포화지방산의 비가 증가한 반면 섭취 6주경과 후에서는 다소 감소하는 경향을 보였으며, 단일불포화지방산의 비는 찹쌀보리빵 군에서는 감소하는 추세이나 밀빵 군에서는 증가하는 경향을 보였다. 또한 혈중 trans 지방산을 비롯한 포화지방산 농도가 낮은 수준이었으며 또한 찹쌀보리빵의 섭취는 밀빵의 섭취에 비해 총 콜레스테롤 농도와 TG/HDL-C의 비를 감소시켰다. 이러한 결과는 찹쌀보리에 함유된 $\beta$-glucan이 trans 지방의 체내 흡수율을 저하시키거나 흡수속도를 느리게 하기 때문으로 사료된다. 따라서 이상의 결과에서 가공식품으로부터 공급되는 trans 지방의 체내 농도를 저하시키고 혈장 총 콜레스테롤 농도를 개선시키기 위해서는 밀가루 보다는 찹쌀보리를 활용한 형태로 섭취하는 것이 바람직하다고 하겠다.

• 한국식품영양과학회지
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• 제38권8호
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• pp.1042-1049
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• 2009

본 연구는 서울지역에 거주하는 건강한 성인 남녀(남자 23명, 여자 10명) 33명을 대상으로 trans 지방 3.75 g을 첨가 한 쌀 빵, 콩 빵과 밀 빵의 섭취가 식후 혈장에서의 지방산과 지질흡수에 미치는 효과를 확인하기 위하여 실시하였다. 시험군은 각 군 당 11명씩 3군으로 분류하였다. 쌀 빵은 밀가루 40 g과 쌀가루 40 g에 trans 지방 3.75 g을, 콩 빵은 콩가루 30 g과 밀가루 50 g에 trans 지방 3.75 g을 첨가하였고, 밀빵은 밀가루 80 g에 trans 지방 3.75 g을 첨가하여 식빵 형태로 각각 조제하였다. 대상자들은 8시간 공복 후 각각의 빵을 섭취하고 0, 1, 2, 3, 4시간 후 채혈하였고, 이는 혈중 지방산과 trans 지방산의 농도 및 지질 수준 분석에 이용되었다. 포화지방산인 C16:0과 C18:0의 수준은 밀 빵을 섭취한 군에서는 시간의 경과에 따라 유의적인 차이를 보였으나 콩 빵과 쌀 빵을 섭취한 군에서는 섭취 후 유사한 수준이었다. C16:0은 콩 빵과 밀 빵을 섭취한 군에서는 섭취 1시간 후에 peak에도달하였으나 시간이 경과함에 감소하는 경향을 보여 섭취4시간 후 밀 빵 군에서는 변화량은 섭취 전의 수준과 유사하였으나 콩 빵 군에서는 섭취하기 전보다도 낮아지는 것으로 나타났다. C18:0은 섭취 1시간 후 콩 빵과 쌀 빵을 섭취한군이 밀 빵을 섭취한 군에 비해 유의적으로 감소하였으나 콩 빵과 쌀 빵 군 간에 유의적인 차이는 없었다. Trans 지방산인 C18:1t과 18:2t는 세 군 모두에서는 섭취 2시간 후 가장 높은 수준을 보였으며 섭취 4시간 후에는 콩 빵 섭취한 군에서의 C18:1t의 혈중 농도가 가장 낮았다. C18:1c은 섭취 4시간 후 콩 빵 군에서 가장 낮았고 쌀 빵 군에서 가장 높았다. 또한 콩 빵의 섭취는 TG의 증가를 효과적으로 억제하였다. 이상의 결과로 미루어 볼 때 콩 빵의 섭취는 밀 빵을 섭취했을 때보다 혈중 trans 지방산을 비롯한 포화지방산 농도와 TG의 수준을 저하하였으며 이것은 콩에 함유된 식이섬유와 생리활성물질들이 지방산과 지방의 체내 흡수율을 저하시키거나 흡수속도를 느리게 하는 때문으로 사료된다. 따라서 trans 지방산을 비롯한 포화지방산과 TG의 체내 농도를 저하시키기 위해서는 밀 빵보다 콩 빵의 형태로 섭취하는 것이 유익할 것으로 사료된다.

• 한국임상약학회지
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• 제16권1호
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• pp.23-27
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• 2006

Doxorubicin (DXR) is a type of anti-cancer drug called an 'anthracycline glycoside', It works by impairing DNA synthesis, a crucial feature of cell division, and thus is able to target rapidly dividing cells. Doxorubicin is a very serious anti-cancer medication with definite potential to do great harm as well as great good. A liquid chromatography-tandem mass spectroscopy (LC-MS/MS) method was developed to identify and quantify DXR in small-volume biological samples. After the addition of internal standard (IS, $5{\mu}L\;of\;1{\mu}M/ml$ daunorubicin methanol solution) into the serum sample, the drug and IS were extracted by methanol. Following vortex for a 1min and centrifugation at 15,000g for 10 min the organic phase was transferred and evaporated under a vacuum. The residue was reconstituted with $350{\mu}L$ of mobile phase and $10{\mu}L$ was injected into C18 column with mobile phase composed of 0.05M ammonium acetate (0.1 M acetic acid adjusted to pH 3.5) and acetonitrile (40:60, v/v). The flow rate was kept constant at $350{\mu}L/min$. The ions were quantified in the multiple reaction mode (MRM), using positive ions, on a triple quadrupole mass spectrometer. The lower limits of quantification for Doxorubicin in plasma and small tissues were approximately 0.5 ng/mL and 0.5 ng/mL respectively. Intra- and inter-assay accuracy (% of nominal concentration) and precision (% CV) for all analytes were within 15%, respectively.