• 한국자원식물학회지
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• 제29권6호
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• pp.635-641
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• 2016

Sweet potato (Ipomoea batatas L.) shoot tips grown in vitro were successfully cryopreserved by encapsulation-vitrification. Encapsulated explants are very easily manipulated, due to the relatively large size of the alginate beads, and a large number of samples can be treated simultaneously. In this study, the effects of sucrose preculture, cryoprotectant preculture, and post-warm recovery media on regrowth, following liquid nitrogen (LN) exposure, were investigated to establish an efficient encapsulation-vitrification protocol for sweet potato. Shoot tips of plants grown in vitro were precultured in 0.3 M sucrose for 2 d before encapsulation. Encapsulated shoot tips were pre-incubated in liquid MS (Murashige and Skoog) medium containing 0.5 M sucrose for 16 h, before preculturing in sucrose-enriched medium (0.7 M sucrose) for 8 h. Shoot tips were osmoprotected with 35% plant vitrification solution 3 (PVS3) for 3 h, before being dehydrated with PVS3 for 2 h at $25^{\circ}C$. The encapsulated and dehydrated shoot tips were transferred to 2 mL cryotubes, suspended in 0.5 mL PVS3, and plunged directly into liquid N. High levels of shoot formation were obtained for the cv. Yeulmi (65.7%) and Yeonwhangmi (80.3%). The regrowth rates of cryopreserved samples in Yeulmi (78.9%) and Yeonwhangmi (91.3%), following culture on ammonium-free MS medium for 5 d, were much higher than those cultured on standard MS medium (65.7% and 80.3%, respectively). This encapsulation-vitrification is a promising method for the long-term preservation of sweet potato.

• Asian Pacific Journal of Cancer Prevention
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• 제16권7호
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• pp.2701-2705
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• 2015

Chondrosarcoma, the second most common type of bone malignancy, is characterized by distant metastasis and local invasion. Previous studies have shown that treatment by pulsed electromagnetic field (PEMF) has beneficial effects on various cancer cells. In this study, we investigated the effects of PEMF applied for 3 and 7 days on the matrix metalloproteinase (MMP) levels in chondrosarcoma SW1353 cells stimulated with two different doses of $IL-1{\beta}$. SW1353 cells were treated with (0.5 and 5 ng/ml) $IL-1{\beta}$ and PEMF exposure was applied either 3 or 7 days. MMP-9 and TIMP-1 levels were measured in conditioned media by enzyme-linked immunosorbent assay. The results were relative to protein levels. Statistical analyses were performed using one-way analysis of variance (ANOVA). P<0.05 was considered significant. PEMF treatment significantly decreased MMP-9 protein levels in human chondrosarcoma cells stimulated with 0.5 ng/ml $IL-1{\beta}$ at day 7, whereas it did not show any effect on cells stimulated with 5 ng/ml $IL-1{\beta}$. There was no significant change in TIMP-1 protein levels either by $IL-1{\beta}$ stimulation or by PEMF treatment. The results of this study showed that PEMF treatment suppressed $IL-1{\beta}$-mediated upregulation of MMP-9 protein levels in a dual effect manner. This finding may offer new perspectives in the therapy of bone cancer.

• Asian Pacific Journal of Cancer Prevention
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• 제16권7호
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• pp.2637-2643
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• 2015

Background: It has been proven that an individuals health behavior is determined through a series of processes. This study aimed to assess the stages of adoption of breast cancer screening, and to identify the factors relating to progress through these stages. Materials and Methods: There were 202 female participants aged 20-59 years who were living in Chungbuk, South Korea. They were informed of the study purpose and agreed to participate. Data were collected from October 2010 to January 2011 by assessing the breast cancer screening stage, health beliefs, socio-demographic factors, and other facilitating factors. The participant current stage of adoption of breast cancer screening was classified using the Precaution Adoption Process Model (PAPM), and the various PAPM stages were compared with each other to identify factors likely to determine progress between stages. The data were analyzed using the ${\chi}^2$-test, ANOVA, Duncan test, and multiple logistic regression. Results: Approximately half of all participants were not on-schedule for breast self-examination and mammography (unaware, 9.4% and 11.4%, unengaged, 8.4% and 5.0%, undecided, 20.3% and 17.8%, decided not to act, 1.5% and 1.0%, decided to act, 13.4% and 15.3%, respectively). The factors likely to determine the progress from one stage to another were age, marital status, exposure to media information about breast cancer, self-efficacy, and perceived severity. Conclusions: These results suggest that it is necessary to develop a tailored message for breast cancer screening behavior.

• Asian Pacific Journal of Cancer Prevention
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• 제17권sup1호
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• pp.31-36
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• 2016

Scientific evidence on all aspects of smoking amongst youth is very important for designing appropriate interventions to reduce smoking among this vulnerable population. This paper describes current access to anti-smoking information among school children aged 13 to 15 years in Vietnam in 2014 and examines its potential impact on preventing smoking initiation. The data used in this paper were obtained from the 2014 Global Youth Tobacco Survey (GYTS) in Vietnam. Students were asked questions about their level of awareness of anti-smoking information from various sources in the past 30 days and about lessons in school regarding the dangers of tobacco use during the last 12 months. Those who have never smoked were asked "whether or not they thought about avoiding cigarettes because of health warnings on cigarette packages" and answers were analyzed in combination with data on access to anti-smoking information from other sources. The prevalence of exposure to antismoking campaigns was high among school children in Viet Nam: 55.3% of current smokers reported thoughts of smoking cessation because of health warnings on cigarette packages; 60.5% of never smokers avoided initiating smoking because of the same health warnings. The potential impact of graphic health warnings to prevent school-aged children from smoking initiation would be stronger if there was concurrent access to anti-smoking programs on the dangers of tobacco use in schools. However, school education for tobacco prevention and control has not been as strong as expected. A more comprehensive school curriculum on tobacco prevention and control is recommended to reinforce antismoking messages among school children.

• 한국인터넷방송통신학회논문지
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• 제13권6호
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• pp.161-166
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• 2013

최근 정보기술 발달로 산업 전 분야에서 IT 융합이 보편적으로 이루어지고 있다. 특히 의료IT 융합분야는 사용자 서비스 측면에서 많은 변화를 보이고 있다. 본 논문에서는 사용자 서비스 측면에 하나의 사례로 기존 종이처방전을 모바일을 이용한 QR코드로 전송할 수 있는 환경을 제안하고자 한다. QR코드는 종이처방전의 출력에서 전달, 보관하는 과정상의 부담을 최소화할 수 있다. 또한 정보 표현을 다양하고 폭넓게 활용할 수 있으며, 정보 복원과 암호화가 가능하므로 개인 정보 노출 및 변조의 위험을 최소화할 수 있는 미디어로 간주된다. 따라서 QR코드의 기능을 의료분야에 유합함으로서 사용자의 편의는 물론 정보의 기밀성, 저장능력을 발휘할 것으로 기대한다.

• Journal of the Korean Academy of Child and Adolescent Psychiatry
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• 제24권2호
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• pp.65-70
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• 2013

Many child actors have appeared in various movies as the Korean film industry continues to evolve. As more children appear in violent and raunchy scenes, there are more concerns about the movie's effect on child actors. In some Western countries, many strategies have been developed for child actors, but for the Korean movie industry, the conditions are still poor for them. Although children who enter the concrete operational period are able to think logically and systematically, they are yet limited by their experiences. Adolescents in the formal operational period try to deal with all of the possibilities and assumptions logically and systematically with freedom from realistic contents and experiences. This period is very important because adolescents become more sensitive to others' feelings and they should develop their ego identity. Several studies have reported the indirect experiences through media including how the movie affected children and adolescents negatively. Depending on the individual's morality, judgment and emotional status, these effects were variable and inconsistent and could be relieved by several interventions. We could anticipate much bigger emotional effect on child actors who are acting directly and then are confronting themselves in the scene. Therefore, we suggest that the emotional effects of the movies on child actors can be managed properly by considering children's cognitive ability and emotional status, and establishing protective strategies before they are exposed to problematic scenes. Of course, it should be followed by evaluating them after the exposure and with follow-up management, if necessary.

• Advances in Traditional Medicine
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• 제2권1호
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• pp.36-40
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• 2002

Effects of hydrogen peroxide $(H_2O_2)-induced$ neurotoxicity were investigated in cultured newborn rat spinal dorsal root ganglion (DRG) neurons after DRG neurons were treated in the media containning various concentrations of $H_2O_2$. In addition, the protective effect of Herba Epimedii (HE) extract against $H_2O_2-induced$ neurotoxicity was examined. Cytotoxic values were determined by the cell viability of living cells using 3-(4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide (MTT) assay. In the present study, exposure of neurons to $H_2O_2$ resulted in a significant cell death in a dose- and time-dependent manners in cultured DRG neurons. The decrement of cell viability by $H_2O_2$ was blocked by HE. These results suggest that the neuroprotective effect of HE against $H_2O_2-induced$ cytotoxicity may result from the prevention of injury induced by $H_2O_2$.

• 한국의류산업학회지
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• 제17권3호
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• pp.315-326
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• 2015

Woman leadership has been remarkably highlighted with women's accelerating entry into society. Domestic and overseas interests about political leaders began with the age of media and they are rapidly spreading worldwide with the development of internet, SNS and blog. Along with this phenomenon, exposure of images has been remarkably increasing and fashion has been also used as a political strategy. However, the research on woman political leaders has been insufficient so this study selected Geunhye Park, Michelle Obama and Hillary Clinton as representative woman leaders for the research. 149 pictures of Geunhye Park, 171 pictures of Michelle Obama and 124 pictures of Hillary Clinton from the articles containing their pictures from Jan. 2002 to Dec. 2013 were analyzed, focusing on their gender. The three woman political leaders' typical images of femininity, masculinity and androgyny were categorized respectively and the period and works in which those images had been expressed were reviewed. Also, fashion styles of the images pursued by each gender were analyzed through their color, material, silhouette, design point, items and accessories. As a result, Geunhye Park had femininity image at the beginning and had masculinity image when she did election campaigns, which led to her current image of androgyny. Michelle Obama uses the image of femininity, masculinity and androgyny simultaneously. It was found that Hillary Clinton emphasized the image of masculinity and androgyny but she emphasizes femininity image these days.

• Asian Pacific Journal of Cancer Prevention
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• 제16권15호
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• pp.6299-6302
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• 2015

Background: The rise in consumption of tobacco products among youth is a public health concern in India. Several studies have shown that advertisements promoting tobacco products influence decisions and behaviour of youth towards smoking. Objective: To ascertain which method of Tobacco Advertising, Promotion and Sponsorship (TAPS) was more influential for initiating tobacco use in youth in India. Materials and Methods: The secondary data of youth (15-24 years) from nationally representative Global Adult Tobacco Survey (GATS) conducted in 2009-2010 was analyzed. Odds ratio and p-value were used to know the association between TAPS and initiation of use of tobacco products among youth. Logistic regression was used to determine the most significant means of TAPS altering the youth's behaviour towards tobacco products. Results: Out of 13,383 youths, 1,982 (14.7%) used smokeless forms of tobacco and 860 (6.38%) used smoke forms. Logistic regression reveals that promotional activities mainly through cinemas (p<0.05) and providing free samples of tobacco products (p < = .001) were most influential means of initiating consumption of tobacco products among youth. Conclusions: The smoking in youth is associated with watching advertisements particularly in cinema and promotional activities like distribution of free samples, coupons and sales on the price of tobacco products. Stronger legislative measures should be enforced to curb promotional advertisements in cinemas and distribution of free samples.

• 동의신경정신과학회지
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• 제11권1호
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• pp.1-18
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• 2000

To study the effects of Ramulus et Uncus Uncariae (REUU) on oxygen free radical-mediated damage by hydrogen peroxide $(H_{2}O_{2})$ on cultured spinal sensory neurons, in vitro assays such as MTT assay, NR assay, neurofilament enzymeimmuno assay (EIA), sulforhodamine B (SRB) assay, assay for lactate dehydrogenase (LDH) activity and assay for lipid peroxidation were used in cultured spinal dorsal root ganglion neurons derived from mice, Spinal dorsal root ganglion neurons were cultured in media containing various concentrations of $H_{2}O_{2}$ for 5 hours, after which the neurotoxic effect of $H_{2}O_{2}$ was measured by in vitro assay. The protective effect of the herb extract, Ramulus et Uncus Uncariae (REUU) against H2O2-induced neurotoxicity was also examined. The results are as follows. 1. In NR assay and MTT assay, $H_{2}O_{2}$ significantly decreased the cell viability of cultured mouse spinal dorsal root ganglion neurons according to exposure concentration in these cultures. An additional time course study was done on these cultures. 2. Cultured spinal dorsal root ganglion neurons which were exposed to various concentrations of $H_{2}O_{2}$ showed a quantitative decrease of neuronal cells by EIA and of total protein by sulforhodamine B (SRB) assay, while they showed an increase of both lipid peroxidation and LDH activity. 3. The effect of Ramulus et Uncus Uncariae (REUU) on $H_{2}O_{2}$ induced neurotoxicity showed a quantitative increase in both neurofilament and total protein, but showed a decrease of lipid peroxidation and LDH activity. These results suggest that $H_{2}O_{2}$ has a neurotoxic effect on cultured spinal dorsal root ganglion neurons from mice and that the herb extract, Ramulus et Uncus Uncariae (REUU), was very effective in protecting $H_{2}O_{2}$ induced neurotoxicity by decreasing lipid peroxidation and LDH activity.