• Korean Journal of Food Science and Technology
• /
• v.47 no.3
• /
• pp.281-285
• /
• 2015

An oleaginous fungus was isolated from soil and identified as Mortierella sp. (M-12) for producing arachidonic acid (AA). Cell disruption methods, extraction methods, and particle sizes of freeze-dried biomass were tested to achieve maximum extraction of total lipids and AA. M-12 grown in glucose yeast media at $25^{\circ}C$ for 7 days contained 35.5% total lipid, and 47% of the total lipid was AA. Lipid extraction yield from wet biomass was shown to be similar to that in a dry state. Maximum lipid extraction was achieved using a mixture of chloroform and methanol (2:1) as an extraction solvent. Different mechanical cell disruption methods did not affect lipid extraction yields. The smaller the particle size of the biomass, the better the lipid extraction yield was observed. Particle size of biomass was shown to more strongly affect lipid extraction than extraction time. The highest AA content was observed in the class of neutral lipids.

• Archives of design research
• /
• v.11 no.1
• /
• pp.161-172
• /
• 1998

The evolution of computing environments caused various dharges in our society. The change cj instruction media is one of these effects. WWW using network techndogy is regarded as a pov.powerful tool for rerrote instruction. The methods of utilizing network technologies in design instrudion and design process rould be diversified comparing with those of other general instruction. Computer graphics has been regarded as a very use!u design tool for its accuracy and rapidty. Network can help us to do creative work using cornplter graphics. The merits of this technology are sharing resources and rraking it easy to roIlaborate. Recent cxxnputer graphics instruction has some defects in oontents and methods. The oontents have a weak relationship with other industrial design subjects. From above, the purpose of this thesis is to use computer graphics and netv.urk technology for supporting basic design instruction. Virtual gallery using WWW can be a cyberspare v.tlere the evaluation of results and the exchange of information take plare. This tool makes it easier to oomrunicate and oollaborate with dassmates. A casestudy-Composition with basic objectswas exea.rted by individual for distributed asynchronous rmde. The results of this thesis are summarized for four factors. Rrst, it was easy to transform idea. Serond, student-oriented working was performed. Third, interaction among students was activated. Fourth, not only final results, but also midterm results was oonsidered for evaluation. These methods also have problems as rerent instruction methods, but it rould be used as a instruction tool to compensate for existing instruction methods.

• Proceedings of the Korean Vacuum Society Conference
• /
• 2013.02a
• /
• pp.588-588
• /
• 2013

Nanoscale noble-metals have attracted enormous attention from researchers in various fields of study because of their unusual optical properties as well as novel chemical properties. They have possible uses in diverse applications such as devices, transistors, optoelectronics, information storages, and energy converters. It is well-known that nanoparticles of noble-metals such as silver and gold show strong absorption bands in the visible region due to their surface-plasmon oscillation modes of conductive electrons. Silver nanocubes stand out from various types of Silver nanostructures (e.g., spheres, rods, bars, belts, and wires) due to their superior performance in a range of applications involvinglocalized surface plasmon resonance, surface-enhanced Raman scattering, and biosensing. In addition, extensive efforts have been devoted to the investigation of Gold-based nanocomposites to achieve high catalytic performances and utilization efficiencies. Furthermore, as the catalytic reactivity of Silver nanostructures depends highly on their morphology, hollow Gold nanoparticles having void interiors may offer additional catalytic advantages due to their increased surface areas. Especially, hollow nanospheres possess structurally tunable features such as shell thickness, interior cavity size, and chemical composition, leading to relatively high surface areas, low densities, and reduced costs compared with their solid counterparts. Thus, hollow-structured noblemetal nanoparticles can be applied to nanometer-sized chemical reactors, efficient catalysts, energy-storage media, and small containers to encapsulate multi-functional active materials. Silver nanocubes dispersed in water have been transformed into Ag@Au nanoboxes, which show highly enhanced catalytic properties, by adding $HAuCl_4$. By using this concept, $SiO_2$-coated Ag@Au nanoboxes have been synthesized via galvanic replacement of $SiO_2$-coated Ag nanocubes. They have lower catalytic ability but more stability than Ag@Au nanoboxes do. Thus, they could be recycled. $SiO_2$-coated Ag@Au nanoboxes have been found to catalyze the degradation of 4-nitrophenol efficiently in the presence of $NaBH_4$. By changing the amount of the added noble metal salt to control the molar ratio Au to Ag, we could tune the catalytic properties of the nanostructures in the reduction of the dyes. The catalytic ability of $SiO_2$-coated Ag@Au nanoboxes has been found to be much more efficient than $SiO_2$-coated Ag nanocubes. Catalytic performances were affected noteworthily by the metals, sizes, and shapes of noble-metal nanostructures.

• The Journal of the Korea Contents Association
• /
• v.8 no.9
• /
• pp.55-65
• /
• 2008

At these information age when the importance of news is of particular emphasis, the field of image-production for the news are being made rapid progressive by high-tech like multi-media, multi-channel digital system. Even experts who have engaged in the work of broadcasting in th field for a long time are perplexed with rapid development in Broadcasting equipments and expression techniques. The field of TV is characterized by the speed of change and the desire of viewers for new and interesting video images. The image expression system applying image line has ever existed as one of conventional image expression methods. Obsolete and old image expressions are paling into significance for viewers who want to access more information in a short time. but The change of image expression systems due to the progressive stream of time has forced existing imaginary to be changed constantly to accommodate the changing interests and expectations of the viewers. Therefore, in this treatise, we need a broad interpretation about the direction of this imaginary line for TV news image in that existing systems of image producing haven’t also been changed and adapted to the stream of time. In these days, image is defined as not only video, but also audio. also We need to reduce the confusion concerning the imaginary line and contribute to a correct understanding images of TV news for not only customers but also producer by extending and applying the concept of imaginary line to image producing.

• Applied Biological Chemistry
• /
• v.50 no.2
• /
• pp.87-94
• /
• 2007

A bacterium which has high enzymatic activities such as amylase, cellulase and protease was isolated from Korean traditional soybean food, doenjang. The isolated bacterium was identified to Bacillus subtilis HS25 by the test of morphological and biochemical properties according to Bergey's Manual of Systematic Bacteriology and API 50 CHL kit, and by the 16S rDNA sequence. The isolated B. subtilis HS25 had a potent antibacterial activity against food born causative or pathogenic bacteria. B. subtilis HS25 is endospore forming cell and contained flagella and abundant viscous material at the out layer of cell wall. It was rod type bacterium $(0.5{\sim}0.8{\times}3{\sim}5{\mu}m)$ having biochemical characteristics such as gram staining(+), catalase(+), oxidase(-) and hydrolysis of esculin(+). The optimal medium compositions for production of antibacterial substance in the B. subtilis HS25 were 1% of soluble starch, 0.5% of yeast extract, 0.5% of peptone and 0.05% of MgCl$_2{\cdot}6H_{2}O$. The optimum temperature and pH of the growth of the B. subtilis HS25 was 35$^{\circ}C$ and pH 7.5, respectively. The antibacterial activity was more high in neutral to a little alkaline pH (6.5-10.5) than in acidic pH. The optimal shaking speed to grow and to produce antibacterial substance of the B. subtilis HS25 was 160${\sim}$200 rpm. The optimal culture time for antibacterial activities of the bacterium were shown to be in the range of 12-36 hr.

• KSBB Journal
• /
• v.8 no.3
• /
• pp.193-199
• /
• 1993

To find out the optimum conditions for indigo cell culture in air-lift bioreactor, effects of media composition including nutrients and precursors of the indigo colorants on the cell growth and characteristics of the cell growth under various cultural conditions were analyzed. Optimum cultural conditions were tested and the growth characteristics were analyzed in external and internal loop type air-lift bioreactors during 14-day culture. Better cell growth was obtained when the inoculum size was higher in the range of 0.5∼2.5% packed cell volume tested. In the sucrose concentration of 2 to 4%, the cell growth was better when the sucrose concentration was 4% (w/w) in both types of reactors. Sucrose was used up in the early stage of exponential phase of growth At the optimum concentration of a Precursor tryptophan at 1 U UW was 3.8 g/l in internal loop bioreactor, and 3.5 g/l in external one after 14 days of cultivation. Addition of indole showed negative effect on cell growth of suspension culture in air-lift biorector culture and cell mass of 2.5 g/l and 2.2 g/l were obtained in external and internal loop bioreactor, respectively. Selected inorganic nitrogen source potassium nitrate showed about 110% increase in cell growth than that of control. DCW was 16.34 g/l under optimum conditions during 14-day cultivation in internal loop bioreactor.

• KSBB Journal
• /
• v.25 no.5
• /
• pp.429-436
• /
• 2010

Keratinase is widely used in certain industrial applications. The present study sought to improve the culture conditions of Bacillus subtilis SMMJ-2 to facilitate mass production of keratinase. Strain SMMJ-2 was irradiated by ultraviolet light and the resulting isolates were tested for keratinase activity. Isolates displaying elevated keratinase activity were selected and used to determine the optimum temperature (24, 30, 37, 45, $55^{\circ}C$) for bacterial keratinase production during a 4 day incubation period. The highest enzyme activity (55 units/mL/min), from a Bacillus subtilis SMMJ-2 mutant (mutant No. 2) was demonstrated following incubation at $30^{\circ}C$. The effects of carbon and nitrogen sources on keratinase production were confirmed by measuring the enzyme activity from the culture broth of the mutant strain cultured in various media containing different carbon source and nitrogen sources during a 4 day period. The optimal medium composition for producing keratinase consisted of 1% glucose, 0.7% $K_2HPO_4$, 0.2% $K_2HPO_4$, and 1.2% soybean meal. Optimal initial pH and temperature for producing keratinase were 7.0 and $30^{\circ}C$, respectively. Keratinases produced by B. subtilis SMMJ-2 and the mutant No. 2 were purified from the culture broth which used soybean meal as a nitrogen source. Membrane ultrafiltration, DEAE-sephacel ion exchange and Sephadex G-100 gel chromatography were used to purify the enzymes. The purified keratinases from both B. subtilis SMMJ-2 and the mutant No. 2 showed single bands and their molecular weights were estimated as 28 kDa and 42 kDa, respectively on SDS-polyacrylamide gel electrophoresis.

• Korean Journal of Food Science and Technology
• /
• v.42 no.6
• /
• pp.768-772
• /
• 2010

In this study, the neuronal cell protective effects of methanol extract from cheonggukjang were evaluated. The proximate composition and total phenolics of the methanol extract were 40.95% crude protein, 22.49% crude fat, 15.99% nitrogen free extract, 7.91% moisture, 6.74% crude ash, 5.92% crude fiber, and 28.43 mg/g of total phenolics. Intracellular ROS accumulation resulting from $H_2O_2$ treatment of PC12 cells was significantly reduced when methanol extract was present in the media compared to PC12 cells treated with $H_2O_2$ only. In a cell viability assay using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium-bromide (MTT), the methanol extract showed protective effects against $H_2O_2$-induced neurotoxicity, and lactate dehydrogenase (LDH) release into the medium was also inhibited. Furthermore, the inhibitory effect of the methanol extract against acetylcholinesterase was dose-dependent.

• Horticultural Science & Technology
• /
• v.18 no.1
• /
• pp.33-38
• /
• 2000

This study was conducted in an effort to meet the need for domestic production of hydroponic media and for medium development using recycled hydroponic rockwool slabs. Two experiments were conducted to evaluate the growth of plug seedlings of petunia 'Romeo' in various mixtures including particles of used rockwool slabs (rockwool particles) and wood chips. In the first experiment, seedlings were grown in plugs filled with mixtures of steam- sterilized rockwool particles and chestnut wood chips, which had been weathered for six months and screened through either 2.8 mm or 5.6 mm sieve, at the mixing ratio of 100:0, 75:25, 50:50, 25:75, or 0:100 (v/v). In the second experiment, seedlings were grown in plugs filled with twenty different mixtures of steam-sterilized particles of used hydroponic rockwool slabs with 4 mm chestnut or pine wood chips, coir, peatmoss, or perlite. In the first experiment seedling growth was not affected by particle size of chestnut wood chips, was superior in the control (commercial plug medium) and in 100% rockwool particles, but was suppressed as mixing ratio of chestnut wood chips increased. In the second experiment, leaf length, and shoot fresh and dry weights were the greatest in rockwool particles+peatmoss+coir treatment, followed by rockwool particles+peatmoss treatment. Leaf length was suppressed as ratio of wood chips increased, especially of chestnut wood chips. Root development was the greatest in rockwool particles+peatmoss+perlite treatment, followed by the control, and rockwool particles+peatmoss+coir treatment. The growth was suppressed as the mixing ratio of rockwool particles, peatmoss, or coir decreased.

• Journal of Bio-Environment Control
• /
• v.12 no.1
• /
• pp.7-11
• /
• 2003

Cellular glass foam (CGF), the reprocessed glass, has a possibility to be used as a medium component in plug culture of horticultural crops due to the its excellent air and water permeability as comparable to perlite. An experiment was conducted to investigate growth of plug seedlings of Lycopersicum esculentum 'Segye' as influenced by irrigation frequency in various medium combinations of CGF (2.0-4.0 mm particle size) and peatmoss. Seeds were sown in 200-cell plug trays, filled with mixtures of CGF and peatmoss either at 33：67 or 25：75 (％. v/v) and were germinated on a fogged propagation bed. The irrigation frequencies used were one, two or three times per every two days. A commercial plug medium (Tosilee medium) was used as the control, and the irrigation frequency in the control was one time per day. Growth of seedlings, and medium pH and EC were measured at 33 days after sowing. The medium composition had little influence on overall growth of seedlings. Irrigation frequency very significant affected number of leaves, leaf area, chlorophyll concentration, fresh and dry weights of shoots and roots, and dry matter. Growth of seedlings was the greatest with the highest irrigation frequency in the 25% CGF＋75% peatmoss mixture.