• Plant Biotechnology Reports
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• 제5권3호
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• pp.245-254
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• 2011

This study describes an efficient protocol for Agrobacterium tumefaciens-mediated transformation of two subgroups of genotype AAA bananas (Musa acuminata cv. Pei Chiao and Musa acuminata cv. Gros Michel). Instead of using suspension cells, cauliflower-like bud clumps, also known as multiple bud clumps (MBC), were induced from sucker buds on MS medium containing $N^6$-Benzylaminopurine (BA), Thidiazuron (TDZ), and Paclobutrazol (PP333). Bud slices were co-cultivated with A. tumefaciens C58C1 or EHA105 that carry a plasmid containing Arabidopsis root-type ferredoxin gene (Atfd3) and a plant ferredoxin-like protein (pflp) gene, respectively. These two strains showed differences in transformation efficiency. The EHA105 strain was more sensitive in Pei Chiao, 51.3% bud slices were pflp-transformed, and 12.6% slices were Atfd3-transformed. Gros Michel was susceptible to C58C1 and the transformation efficiency is 4.4% for pflp and 13.1% for Atfd3. Additionally, gene integration of the putative pflp was confirmed by Southern blot. Resulting from the pathogen inoculation assay, we found that the pflp transgenic banana exhibited resistance to Fusarium oxysporum f. sp. cubense tropical race 4. This protocol is highly advantageous to banana cultivars that have difficulties in setting up suspension cultures for the purpose of quality improvement through genetic transformation. In addition, this protocol would save at least 6 months in obtaining explants for transformation and reduce labor for weekly subculture in embryogenic cell suspension culture systems.

• Natural Product Sciences
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• 제24권1호
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• pp.66-70
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• 2018

Helminthostachys zeylanica is a rare plant grows in lightly shaded areas. The fern was traditionally used as antipyretic and antiphlogistic agents. This study was aimed to evaluate the antibacterial potential of H. zeylanica on foodborne Bacillus cereus. The chemical composition of its ethanolic extract was also determined. The plant samples were collected at Kampung Kebun Relong, Kedah, Malaysia. The ethanolic extract showed significant inhibitory activity on B. cereus with a sizeable clear zone detected on disc diffusion assay. On broth microdilution assay, the MIC of the extract on B. cereus was 6.25 mg/ml and the MBC was 12.5 mg/ml. The inhibitory activity of the extract on B. cereus was bactericidal. In the growth dynamic study, the antibacterial efficacy of the extract was concentration dependent, where a lower colony forming unit count was obtained with increased extract concentration. The SEM micrograph of extract treated B. cereus cells showed invaginations of cell wall. The bacterial cell structure collapsed after 24 h exposure to the extract. The GCMS analysis of the extract showed that the major constituents of the extract were phenol (36.26%) and quercetin (29.70%). This study is important as it shows the potential use of H. zeylanica as an effective agent to control B. cereus related infections.

• 대한기계학회논문집A
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• 제41권11호
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• pp.1035-1045
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• 2017

얼굴 모양 및 목소리를 이용하는 방법을 포함하여 연령 및 성별을 분류하는 다양한 방법이 연구되고 있다. 그러나 얼굴 기반 방법은 원거리에서 인식률이 급격히 감소하고, 오디오 기반 방법은 잡음이 많은 환경에서는 적용하기 어렵다. 대조적으로 보행 기반 방법은 대상자가 카메라에 촬영만 되면 인식이 가능하다. 기존 연구에서 카메라의 시점은 측면에서만 볼 수 있어서 실제 환경에서 일반 보행과는 현실적으로 차이가 발생했다. 본 연구에서는 일반 보행 데이터를 이용하여 연령과 성별을 분류할 수 있도록 RGB-D 센서로부터 획득된 골격 모델을 이용한 특징 추출 방법을 제안한다. 실험 결과는 제안된 방법이 실제 환경에서 효율적임을 보여준다.

• The Journal of Korean Physical Therapy
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• 제25권3호
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• pp.126-131
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• 2013

This case report described a single case of adult stoke patient due to Moyamoya disease through long-term follow-up observation, which included his demographics, brain images, and change of motor function and functional activities. The subject was the 54-year-old male diagnosed with left hemiparesis from a stroke due to multifocal encephalomalacia in both hemispheres. At the time of the stroke attack, he took brain surgery intervention including external ventricular drain. Physical and occupational therapy for stroke rehabilitation were admitted including muscle strengthening exercises, functional activity/ADL training, neurofacilitative techniques with bobath or proprioceptive neuromuscular facilitation concepts, and compensatory strategy. Patient's MRI showed that right frontal lobe, right peri-ventricular area, left parietal, and left occipital lobes were damaged, and MRA showed that abnormal collateral vessel was richly developed in both hemispheres by occlusion of proximal internal carotid arteries in both sides. His motor strength was improved from poor to good grade in all of upper and lower limb motions, that MBC was improved from stage 1 to stage 5. In FAC and barthel index, at the initial evaluation, he could not perform any functional movement, but his FAC and barthel index were on 3 and 14 points at present, respectively. During long-term follow-up for approximately 4 years, the subject's functional motor ability was improved, as similar with recovery progression of usual stroke patient. We believe that this single case report will provide clinical information and concern regarding Moyamoya disease with physical therapist, in terms of such as epidemiology, pathogenesis, diagnostic procedures, clinical features, recovery process, and prognosis.

• 동의생리병리학회지
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• 제19권5호
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• pp.1233-1237
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• 2005

Catechin products in green tea extract was prepared to investigate antibacterial activity on the pathogenic bacteria. Survival of pathogenic bacteria (MASA - methicillin resistant Staphylocouus aureus, E.coli O157 and S. typhimurium Sal-13) in tryptic soy agar(TSA) containing Catechin products powder incubated at various concentration was evaluated. TSA containing $0{\sim}2%(w/v)$ of Catechin products was inoculated approximately $10^4\;CFU/ml$ of pathogenic bacteria and incubated at $37^{\circ}C$ for 24 hours. The plate counting technique and clear zoon test were used to test survival effect of the Catechin products. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) was derived from the survival curves of pathogenic bacteria. S. typhimurium Sal-13 was the most sensitive strain to Catechin products. This result suggested that Catechin products can be used as an effective natural antibacterial agent.

• 방송공학회논문지
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• 제22권1호
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• pp.95-106
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• 2017

디지털 방송 통신 기술의 발전에 따라 고화질 방송 서비스와 함께 컴패니언 스크린 서비스에 대한 관심이 높아지고 있다. 개인 모바일 디바이스 사용이 확산됨에 따라 주 스크린에서 양질의 방송을 제공 하는 것 뿐 아니라 세컨드 스크린에서 부가적인 가치를 창출하는 것도 중요하다. 본 논문에서는 사용자의 컨텍스트에 의거하여 적응적인 컴패니언 서비스를 만드는 플랫폼과 관련 저작 도구를 제안한다. 이를 위해 사용자의 상황에 기반하여 원격으로 리소스를 모으고 조합하여 적응적인 컴패니언 스크린 서비스를 공급하는 플랫폼을 설계하였고 실제 서비스 환경에 맞게 구현하였다. 제안하는 플랫폼은 사용자의 다양한 컨텍스트와 사업자의 요구를 고려하여 양질의 컨텐츠를 실시간 구성하고 사용자에게 제공할 수 있으며 플랫폼 기반 저작 도구는 숙련되지 않은 디자이너와 서비스 기획자도 적응적 컴패니언 스크린 서비스를 쉽고 빠르게 만들 수 있도록 한다. 설계한 플랫폼을 이용하여 인터랙티브 서비스 제공이 가능한 한국 UHD IBB 표준을 준수하는 컴패니언 스크린 서비스 컨텐츠를 만들고 시뮬레이션 하였다.

• 한국언론정보학보
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• 제56권
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• pp.7-25
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• 2011

이 연구는 한국 사회에서 신문이 방송을 겸영함으로써 여론 다양성에 위기를 불러온다는 전망과, 정반대로 여론 독과점 해소에 기여한다는 주장이 학계에서 대립하며 소통이 막혀있는 현실에 주목하고 과학적 접근을 통해 학문적 토론을 점화하는 데 목적이 있다. 신문과 방송 겸영에 따른 여론 다양성 문제를 과학적으로 분석하기 위해 실제로 방송을 겸영할 조선일보, 중앙일보, 동아일보가 사실에 근거해 여론을 형성해왔는지 '신방 겸영론'을 중심으로 분석했다. 이어 세 신문사가 신방겸영을 주장한 논리적 근거로 제시한 규제 완화와 경제 살리기, 일자리 늘리기 보도들에서 다양한 관점이 드러났는지, 아니면 특정 프레임(frame)이 지배적이었는지 실증적으로 탐색했다. 이어 세 신문사 방송이 자리할 객관적 조건이 한국 공론장(public sphere)의 여론독과점과 어떤 관련성이 있는지 여론조사와 설문조사를 근거 자료로 분석했다. 과학적 분석 결과 '조중동 방송'은 여론 독과점을 심화시킬 수밖에 없다고 전망한 이 논문은 그렇지 않다고 판단하는 연구자들과 학문적 토론을 기대하고 있다.

• 복식문화연구
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• 제17권6호
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• pp.1112-1128
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• 2009

The purpose of this study was to develop royal costume design with contemporary taste depending on the character of hero, Lee San, and time flow of TV historical drama and research design discrimination and creative expression. As study methods, the literature study using books and theses concerning costume, fine art, culture and history were used for theoretical background and the empirical study method manufacturing actual costume on the basis of literature and relic were used for dress manufacture. The design concept of costume was modern & chic & clean. The costume was designed through straight silhouette, clean & bright colors excluding prime colors, style transformed to permanent straight pleats on the basis of chulik, 100% silk material focusing on high quality and pattern & decoration of the age of Three Kingdoms. As results, the designs of princess' official rob, day dress and chulik, and emperor's official robe, chulik, military dress and chun-dam-bok were developed. The design discrimination depending on social status change. In the period of princess, black, white, silver and blue were used but in the period of emperor, red, gold and black were used. It was expressed through dragon pattern and extended length used in cloth. The design discrimination depending on character change was visualized through color. Although it is not consistent with historical investigation, contemporary fashion design elements were added to royal costume through color contrast of black & white, permanent straight pleats, velcore, wristlet, extended length and layered-look.

• 한국임상수의학회지
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• 제25권5호
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• pp.346-354
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• 2008

The combined effects of EDTA-Tris and eighteen antimicrobial agents have been evaluated in eight clinically isolated methicillin-resistant bacteria (Staphylococcus aureus, Escherichia coli, Streptococcus uberis and Streptococcus agalactiae) from bovine mastitis. The antimicrobial activity was evaluated by measuring the minimal bactericidal concentration (MBC) for the antibiotics alone or in combination with EDTA-Tris in Mueller-Hilton broth and milk. Combined use of EDTA-Tris and antibiotics potentiated or antagonized activity of antibiotics against mastitic pathogens. Milk increased the antibiotic potency of erythromycin and spiramycin on S. aureus. Culture in milk changed patterns of EDTA-Tris combinational effects compared with that in standard Mueller-Hilton broth. Combined with EDTA-Tris in milk, synergic effects were observed in colistin, dihydrostreptomycin, kanamycin, erythromycin, gentamycin, oxytetracycline, streptomycin to E. coli, Str. uberis, and Str. agalactiae. However, significant antagonistic effects of milk on antibiotic susceptibility in combination with EDTA-Tris were noted in neomycin, streptomycin, penicillin, roxithromycin, and amoxicillin. This study indicates that combination therapy of EDTA-Tris with antibiotics in bovine mastitis should be used with caution because of the possible antagonistic effects of antibiotic combination with EDTA-Tris on mastitic pathogens. In addition, antibiotic susceptibility test in combination with EDTA-Tris in milk culture condition can be benefit in search of effective treatment regimen for some antibiotic-resistant bacteria of mastitis.

• 한국식품조리과학회지
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• 제33권3호
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• pp.256-263
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• 2017

Purpose: Essential oils are secondary metabolites of herbs and have antibacterial activities against foodborne pathogens. However, their applications for food protection are limited due to the hydrophobic and volatile natures of essential oils. Methods: In this study, essential oil nanoemulsions of rosemary and lavender were formulated with non-ionic surfactant Tween 80 and water using ultrasonic emulsification, and their antibacterial effects were determined. Results: The antibacterial activities of nanoemulsions were evaluated against 12 strains of 10 bacterial species, and significant antibacterial effects were observed against four Gram-positive and four Gram-negative bacteria but not against Streptococcus mutans and Shigella sonnei. In the disc diffusion test, the diameter of the inhibition zone proportionally increased with the concentration of nanoemulsions. Using cell turbidity measurement, minimum bactericidal concentration (MBC) of the nanoemulsions, which is the lowest concentration reducing viability of the initial bacterial inoculum by ${\geq}99.9%$, was significantly higher than the minimum inhibitory concentration (MIC) of the nanoemulsions. The largest bactericidal effects of lavender and rosemary essential oil nanoemulsions were observed against S. enterica and S. aureus, respectively. Conclusion: Nanoemulsion technique could improve antibacterial activity of essential oil nanoemulsions by increasing the solubility and stability of essential oils. Our findings shed light on the potential use of essential oil nanoemulsions as an alternative to chemical sanitizers in food protection.