• Journal of Korean Society of Forest Science
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• v.87 no.3
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• pp.334-340
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• 1998

This research was conducted to identify plant regulatory regions by gene tagging method. A promoterless GUS coding sequence was introduced to Populus nigra ${\times}$ P. maximowiczii via Agrobacterium strains(LBA4404/EHA101), and putative transgenic poplars were selected by culturing on medium containing G418($60mg/{\ell}$) and by GUS assay. Among them one positive plant was to amplify the native sequences flanking to the introduced GUS gene in plant genome by inverse PCR method and from this 730 by DNA product was obtained. After subcloning and sequencing, it has 88% homology to the Eucalyptus gunnii CAD(cinnamyl alcohol dehydrogenase) gene. The GUS gene fused with the putative promoter reinserted into poplar leaves by particle bombardment method to test the funtional promoter activity. Upon staining with X-gluc, many blue spots appeared on the leaf segments bombarded by the chimeric gene 2-3 days, thus the isolated DNA fragment contain some possible coding region as well as a putative regulatory sequences of poplar CAD gene.

• Journal of Korean Society of Forest Science
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• v.21 no.1
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• pp.35-38
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• 1974

In order to investigate reproductive capacity of genus Lespedeza 5 spp,; L. bicolor, L. maximowiczii, L. japonica var. intermedia, L. angustifolioides, and L, cyrtobotrya, their hights, diameter at the ground level, and the number of sprouts were compared with each other at the end of October during investigating years from 1970 to 1972. For measuring of invested items, 6 stocks which the one-year aged stem were cut above ground were randomly planted per square meter with six replications at the spring of 1970. After hights, diameter, and the number of sprouts were measured at the end of October every year, the stem were cut above ground at the end of November from 1970 to 1972. The results are as follows: 1. The reproductive of the stems showed statistically significant differences at 1% level by species and stock ages. L. bicolor showed the best reproductive capacity of them. 2. The sprouting ability was revealed that L. maximowiczii showed the best of them. 3. The reproductive capacity of stem, diameter, and number of sprouts increased with aging of stock up to 4 years on the investigated 5 species.

• Korean Journal of Environment and Ecology
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• v.23 no.1
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• pp.22-29
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• 2009

To investigate the vegetation structure of mountain valley from Cheonwangbong to Seonneotang, 23 plots($400m^2$) set up with random sampling method were surveyed. Two groups of Abies koreana community, Fraxinus mandshurica-Acer pseudo-sieboldianum community were classified by cluster analysis. Abies koreana was a major woody plant species in the valley area from Cheonwangbong to Mapogpo, and Pinus koraiensis, Acer pseudo-sieboldianum and Sorbus amurensis was partly occupied. High positive correlations was proved between Picea jezoensis and Sorb us amurensis, Taxus cuspidata, Fraxinus sieboldiana, Kalopanax pictus; Betula costata and Betula platyphylla var. japonica, Corn us controversa; Abies koreana and Pinus koraiensis; Stephanandra incisa and Betula ermani, Acer tegmentosum, Prunus sargentii; Acer pseudo-sieboldianum and Betula ermani; Fraxinus mandshurica and Deutzia prunifolia, Acer tschonoskii var. rubripes; Sorbus amurensis and Kalopanax pictus; Betula platyphylla var.japonica and Cornus controversa; Lindera erythrocarpa and Lespedeza maximowiczii, Quercus serrata, Styrax obassia; Lindera obtusi/oba and Abies holophylla; Carpinus laxiflora and Lespedeza maximowiczii; Fraxinus sieboldiana and Acer tschonoskii var. rubripes, Pinus koraiensis, Taxus cuspidata; Acer tschonoskii var. rubripes and Taxus cuspidata; Quercus mongolia and Rhododendron schlippenbachii; Quercus serrata and Styrax obassia, and relatively high negative correlations was proved between Abies koreana and Deutzia prunifolia; Lindera obtusi/oba and Pinus koraiensis. Species diversity(H') of investigated groups was ranged $2.7478{\sim}2.9573$.

• Journal of Korean Society of Forest Science
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• v.35 no.1
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• pp.15-18
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• 1977

In order to investigate reproductive capacity of genus Lespedeza 5 spp.; L. bicolor, L. maximowiczii, L. japonica var. intermedia, L. angustifolioides, and L. cyrtobotrya, their hights, diameter at the ground level, and the number of sprouts were compared with each other at the end of October during investigating years from 1970 to 1976. For measuring of invested items, 6 stocks which the one-year aged stem were cut above ground were randomly planted per square meter with six replications at the spring of 1970. After hights, diameter, and the number of sprouts were measured at the end of October every year, the stem were cut above ground at the end of November from 1970 to 1976. The results are as follows: 1. The reproductive of the stems showed statistiaclly significant differences at 1% level by species and stock ages. L. bicolor showed the best reproductive capacity of them. 2. The sprouting ability was revealed that L. maximowiczii showed the best of them. 3. The reproductive capacity of stem, diameter, and number of sprouts increased with aging of stock up to 5 years on the investigated 5 species.

• Journal of Korean Society of Forest Science
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• v.79 no.3
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• pp.278-284
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• 1990

We have demonstrated expression of bacterial genes transferred into cells of Populus nigra ${\times}$ P. maximowiczii by A. tumefaciens strain 6044 (pGA 472). We determined the optimum concentration of kanamycin sulfate for effective selection of punctured leaf transformed using Agrobacterium binary vector pGA 472 containing a neomycine phosphotransferase gene (NPT-II) which confers kanamycin resistance. The combination of cefotaxime (200mg/l) and carbenicillin (300mg/l) showed good performance of discarding Agrobacterium from inoculated punctured leaf. A relatively low concentration (10mg/l) of kanamycin sulfate inhibited callus and shoots induction from punctured leaf. Number of shoots regenerated from co-cultured punctured leaf was 3.0 on MS basal medium supplemented with 10 mg/l kanamycin sulfate, while that of not co-cultured punctured leaf was none. The regeneration rate was 10% from the punctured leaf co-cultured on MS medium with 10 mg/l kanamycin. Regenerated shoots are developing from micropropagation for Southern blot analysis and inheritance of the kanamycin resistance trait (NPT-II).

• Korean Journal of Environment and Ecology
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• v.3 no.1
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• pp.42-50
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• 1989

A valley and a ridge forest in Mt. Gaya area was studied to investigate forest structure in relation to altitude and part of slope. Sixty-three quadrats were set up in the valley forest along altitude of 600m to 1,000m and part of slope, and thirty-eight quadrats were set up in the ridge forest along altitude of 700m to 1,430m. According to the importance values, the valley forest was Quercus mongolica-Lespedeza maximowiczii community and the ridge forest was Pinus densiflora, Quercus mongolica-Rhododendron mucronulatum community. Similarity index between the valley forest community and the ridge forest community was 37.2%. Shannon's species diversities of the valley forest community and the ridge forest community were 1.3402 and 1.0098, respectively. According to importance values by crown stories and DCA ordination, successional trends of tree species may be from Pinus densiflora and Pinus koraiensis through Quercus mongolica to Quercus serrata and Carpinus laxiflora. As going from the lower part to upper part of the slope in the valley forest, the importance values of Quercus mongolica, Quercus aliena, Rhododendron mucronulatum and Lespedeza maximowiczii increased while those of Carpinus laxiflora and Fraxinus rhynchophylla decreased. With increasing elevation in the valley and ridge forest, the importance value of Pinus densiflora decreased while that of Quercus mongolica increased. In the valley forest, densities of canopy and shrubstratum increased as increasing elevation, and the number of species and species diversity decreased as increasing elevation and going from the lower part to the upper of slope. The range of similarity indices between parts of the slope, and the elevation belts of 100m in the valley forest were 66.6-69.2 and 25.9-79.8%, respectively. In the ridge forest, density and basal area of canopy tended to decreased as increasing elevation, and the range of similarity indices between elevation belts of 100m was 27.9-98.2%.

• Korean Journal of Environment and Ecology
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• v.20 no.2
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• pp.114-121
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• 2006

To investigate the vegetation structure of mountain ridge from Miwangjae to Cheonwhangbong, 11 plots($2000m^2$) set up with random sampling method were surveyed. Three groups of Quercus mongolica community, Quercus mongolica-Styrax japonica community, Q. mongolica-Pinus densiflora community were classified by cluster analysis. Quercus mongolica was a major woody plant species in the ridge area from Miwangjae to Cheonwhangbong, and Styrax japonica and Pinus densiflora was partly occupied. High positive correlations was proved between Prunus sargentii and Euonymus alatus for. ciliato-dentatus; Quercus serrata and Euonymus alatus; Sorbus alnifolia and Euonymus alatus, Magnolia sieboldii; Acer pseudo-sieboldianum and Euonymus alatus for. ciliato-dentatus; Rhododendron schlippenbachii and Euonymus alatus for. ciliato-dentatandus; Euonymus alatus and Lindera obtusiloba, Castanea crenata, Lindera erythrocarpa; Lespedeza bicolor and Rhododendron schlippenbachii; Castanea crenata and Magnolia sieboldii; Magnolia sieboldii and Lindera erythrocarpa, and relatively high negative correlations was proved between Rhododendron schlippenbachii and Euonymus alatus; Lespedeza bicolor and Castanea crenata; Stephanandra incisa and Magnolia sieboldii; Rhododendron yedoense var. poukhanense and Lindera erythrocarpa; Euonymus alatus for. ciliato-dentatus and Magnolia sieboldii; Fraxinus sieboldiana and Sapium japonicum; Prunus sargentii and Lespedeza maximowiczii; Lespedeza maximowiczii and Lindera erythrocarpa. Species diversity(H') of investigated groups was ranged $2.3225{\sim}2.7721$, and it was relatively low value compared to that of mountain ridge area of other national parks.

• Journal of Korean Society of Forest Science
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• v.79 no.4
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• pp.407-412
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• 1990

Morphological characters on the leaf nectar gland of Korean Prunus 4 species and 1 form were observed by SM. The results were summarized as follows ; The shapes of nectar gland on the plane figure were two, the circular and the irregular ovate. The species of circular shape were P. maackii, P. maximowiczii, P. sargentii and P. yedoensis. The irregular ovate shape was only one species, P. pendula for. ascendens. The shapes of nectar gland on the side figure were the reentrant apices and the rounded. The reentrant apices were two shapes. First, the species of the center reentrant was three, P. maackii, P. sargentii and P. yedoensis. Second, the species of the irregular reentrant was P. pendula for. ascendens. The rounded apex was only one species, P. maximowiczii. Especially on the P. pendula for. ascendens, the upheaval situation on surface of leaf blade was due to the sinking of reverse part of leaf blade at the nectar gland. The point of this abnormal shape was well distinguished from the other species. On the frequency by the part of leaf with nectar gland, the species to be observed only at the leaf base was P. pendula for. ascendens, the other species was observed two part of leaf base and petioles. In this study, some Prunus species were well keyed out on the basis of the morphological characters of leaf nectar gland.

• Journal of Korean Society of Forest Science
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• v.90 no.6
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• pp.734-741
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• 2001

The two-month-old rooted-cuttings of Populus alba ${\times}$ glandulosa, P. euramericana and P. nigra ${\times}$ maximowiczii clones were exposed to livestock waste water - one of major water pollutants, and ground water in order to determine the effects of livestock waste water on growth response and absorption capacity of the species. For this purpose, 5 clones of each species were used. In all the species, the height growth of rooted-cuttings was better in livestock waste water treatment than in ground water. Of all the poplar species compared, the height growth was best in P. alba ${\times}$ glandulosa. In the cases of P. euramericana and P. nigra ${\times}$ maximowiczii, the height growth in the livestock waste water treatment was statistically different among clones, whereas there was no significant difference among P. alba ${\times}$ glandulosa clones. Aboveground biomass such as leaf and shoot dry weight of all the species increased in the livestock waste water treatment, while root dry weight decreased. In addition, chlorophyll contents in leaf of all the poplar species increased in the livestock waste water treatment. All the poplar trees showed temporal variation in the absorption amount of livestock waste water during the experimental period. In all the poplar species, the absorption amount of livestock waste water was less than that of ground water. Of 3 poplar species, P. alba ${\times}$ glandulosa was best in the absorption capacity of livestock waste water. Of all 15 poplar clones compared in this study, the 72-16 clone of P. alba ${\times}$ glandulosa showed the best absorption capacity.

• Journal of the Korean Institute of Landscape Architecture
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• v.15 no.2
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• pp.67-78
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• 1987

This study was executed to measure the user\`s impact natural environment in National Park Campsite. Therefore, we choose Mt. Chiak National Park and the campsite in the district of Temple Ku-Ryong as a model of analysis, which is made by ecological approach, the number of the user has influence on the environment near the campsite. The result are as follows : 1. The more the number of users becomes, the more Na, Ca, Mg ion concentration and pH in the soil increase and the less K ion becomes. 2. Litter and humus depth, litter coverage, herb coverage, soil hardness, the surface of the ground layer and tree species numbers, density and coverage of lower layer shows a sensitive reaction to the number of the users. 3. The research shows that the tolerant trees against campsite impact are Lespedeza maximowiczii, Zanthoxylum schinifolium Staphylea bumalda, Smilax sieboldii, Quercus aliena, Euonymus oxyphyllus, Weigela subsessilis and Securinega suffruticosa, and the sensitive trees are Stephanandra incisa, Rubus trichocarpa, Rubus crataegifolius, Rhododendron mucronulatum, Styrax obassia, Acer palmatum, Lindera obtusiloba, Rhododendron suhlippenbachii, Rhus japonica and Callicarpa japonica. 4. The high density of the users made the simplication of a vegetation structure. So, as the number of the user increase the species showed severe heterogeniety between the heavy-use and nonuse site. 5. As considering the landscape management about campsite and surrounding area on the way of analysis of national landscape aspect and succession sere according to ecological aspect, it may be desirable that Pinus densiflora forest be conserved as Pinus densiflora landscape and mixed forest produce natural scenery with succession sere.