• Journal of fish pathology
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• v.18 no.1
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• pp.19-28
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• 2005

Scuticociliatosis has badly settled one of most damaging diseases during the seedling production process of olive flounder. Paralichthys olivaceus in Korea. We isolated a new type of Scuticociliate from flounder. The parasite metamorphoses to ciliate and cyst phases with each other by environmental changes and survive for a relatively long span. The ciliate was measured average 41.8 ${\mu}m$ in length and 21.0 ${\mu}m$ in width, and cyst was 17.0 ${\mu}m$ and 13.5 ${\mu}m$, respectively. Nutritional condition was determined as a major parameter of metamorphosing between ciliate and cyst stages. The ciliate transforms to a cyst stage because of food shortage, and the cyst returns to a ciliate stage with a favorite environmental condition and shows active growth and reproduction. The ciliate multiplied at the maximal density of $2.9 {\times} 10^5 {m\ell}^{-1}$cells in vitro cultivation at $15 ^{\circ}C$temperature using MS BHI medium and bacterial food sources. The ciliate could be proliferated at a 2.5 to $30 ^ \circ}C$ temperature range, pH 6 to 9, and 1 to 55 ppt salinity. Particularly, it survived over one week at $0 ^{\circ}C$temperature showing a high resistance against unfavorable environmental conditions. And the cyst survived for 320 days in the condition of $5 ^{\circ}C$with no feeding, but its survival period was markedly shortened in higher temperature conditions. The chemotherapeutants (formalin and hydrogen peroxide) were clarified as effective chemicals against the ciliate during in vitro trials, but the effect of therapeutants differed in proportion, depending upon the density and the bathing time of chemical compounds.

• Journal of Veterinary Clinics
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• v.24 no.4
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• pp.597-602
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• 2007

Furosemide is a potent diuretic used in the horse for the prophylaxis of exercise-induced pulmonary hemorrhage(EIPH), and in a variety of clinical condition. The purpose of this study was to set up the effect of furosemide in Thoroughbred horses, and to examine the time of maximal effect of furosemide to prevent the EIPH. The animals of the experiment were 4 Thoroughbred racing horses(female, average 6 yrs, BW $507{\pm}80.2kg$) in Jeju stud farm and the experiment was cross-over design between furosemide(1 mg/kg IV) and normal saline(0.1 ml/kg, IV) administration. We investigated the changes of clinical signs(body weight, skin turgor, capillary refilling time, jugular distensibility), CBC(PCV RBC), serum chemistry(TP, AST, GGT, glucose, LDH, BUN and creatinine) and electrolytes ($Na^+,\;K^+,\;Cl^-,\;Ca^{2+}$) on 0, 30 mins, 1, 2, 4, 8 and 24 hrs in furosemide and saline administration roup, respectively. The weight decreased significantly(P<0.05) in 4 hrs after furosemide treatment, but skin turgor, CRT, jugular distensibility remained unchanged throughout the experimental period. RBC and PCV decreased after furosemide administration but soon recovered. TP increased to the highest level in 30 mins after furosemide administration and recovered in 8 hrs. Furosemide administration resulted in increases in glucose, AST, GGT, LDH, BUN, creatinine(P<0.05) and decreases in $Ca^{2+},\;Na^+,\;K^+,\;Cl^-$ (P<0.05). All of values were within normal range throughout the experimental period. There was the alteration of blood and serum chemistry after furosemide administration, all of values were within normal range. And the best time of furosemide administration will be 30 minutes before the racing in order to preventing EIPH.

• Journal of the Korean Dietetic Association
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• v.11 no.1
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• pp.86-94
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• 2005

This study was conducted to examine bone mineral density and factors which effect on bone mineral density such as daily nutrients intake, age, menarch age and physical condition among healthy female college students in Daegu area from April 20, to May 20, 2004. 1. Average age was 20.5$\pm$0.96 years old, average height was 160.9$\pm$4.30㎝, and average weight was 55.9$\pm$7.67㎏. Body mass index was 21.6$\pm$2.91㎏/㎡, body fat was 25.6$\pm$5.79％, menarche age was average 12.5$\pm$1.1 years old and WHR(waist/hip circumference ratio) was 0.8$\pm$0.01㎝/㎝. 2. Average level of bone mineral density(T-score -0.56$\pm$0.91) was in normal range. But, 11 persons(24.4％) are over T-score -1.0, 33 persons(73.4％) were within -1.0 - -2.5 and one person(2.2％) was under -2.5. It is very anxious level for Osteopenia-low bone mass, as research result shows 73.4% of the subject of examine on the level of Osteopenia. 3. Daily calorie intake was 2,550㎉ and each nutrient intake, compared to the seventh recommended dietary allowances for korean, was as follows ; Calorie 112％, protein 123％, calcium 78％, phosphorus 137％, iron 68％, vitamin A 101％, vitamin $B_1$ 141％, vitamin $B_2$ 95％, niacin 107％, vitamin C 128％ and zinc 120％. The ratio of calcium to phosphorus(Ca/P) is 0.66, low compared to RDA, but phosphorus intake is so high compared to RDA that precautions should be taken. The ratio of calcium to protein(Ca/Protein) is 8.55. 4. Menarche age and bone mineral density of calcaneus showed positive correlation and body mass index(BMI) indicated positive correlation. Age, height, weight. WHR and physical activity coefficient all do not indicate any significant correlation with bone mineral density. 5. Intake of Calorie, Ca, Ca/p ratio, carbohydrate and fat intake were positively correlated and, protein was negatively correlated, and Fe, Na, P and cholesterol were negatively correlated with BMD. These results indicate that average bone mineral density of subjects was in normal range, but subjects in the stage of osteopenia-low bone mass are many and bad effects are expected to have on their bone mineral density after menopause. Therefore, in order reach maximal bone mineral density, they should improve the balance between calcium and phosphorus and reduce salt intake. And it is thought that education and profound studies on relevant factors affecting the genesis of bone mineral density should be made.

• Journal of the Institute of Electronics Engineers of Korea CI
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• v.41 no.2
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• pp.23-32
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• 2004

The query optimizer's important task while a query is invoked is to estimate the fraction of records in the databases that satisfy the given query condition. The query result size estimation in spatial databases, like relational databases, proceeds to partition the whole input into a small number of subsets called “buckets” and then estimate the fraction of the input in the buckets. The accuracy of estimation is determined by the difference between the real data counts and approximations in the buckets, and is dependent on how to partition the buckets. Existing techniques for spatial databases are equi-area and equi-count techniques, which are respectively analogous in relation databases to equi-height histogram that divides the input value range into buckets of equal size and equi-depth histogram that is equal to the number of records within each bucket. In this paper we propose a new partitioning technique that determines buckets according to the maximal difference of area which is defined as the product of data ranges End frequencies of input. In this new technique we consider both data values and frequencies of input data simultaneously, and thus achieve substantial improvements in accuracy over existing approaches. We present a detailed experimental study of the accuracy of query result size estimation comparing the proposed technique and the existing techniques using synthetic as well as real-life datasets. Experiments confirm that our proposed techniques offer better accuracy in query result size estimation than the existing techniques for space query size, bucket number, data number and data size.

• 한국생물공학회:학술대회논문집
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• 2005.04a
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• pp.327-333
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• 2005

Dihydroxy-acid dehydratase (DHAD, 2,3-dihydroxy-acid hydrolyase, EC 4.2.1.9) is one of the key enzymes involved in the biosynthetic pathway of the branched chain amino acid isoleucine and valine. Although the enzyme have been purified and characterized in various mesophiles including bacteria and eukarya, the biochemical properties of DHAD has bee not yet reported from hyperthermophilic archaea. In this study, we cloned, expressed, and purified a DHAD homologue from the thermoacidophilic archaeon Sulfolobus solfataricus P2, which grows optimally at $80\;^{\circ}C$ and pH 3, in E. coli. Characterization of the recombinant S. solfataricus DHAD (rSso_DHAD) revealed that it is the dimeric protein with a subunit molecular weight of 64,000 Da in native structure. rDHAD showed the highest activity toward 2,3-dihydroxyisovaleric acid among 17 aldonic acid substrates Interestingly, this enzyme also displayed 50 % activities toward some pentonic acids and hexonic acids when compared with the activity of this enzyme to the natural substrate. Moreover, rSso_DHAD indicated relatively higher activity toward D-gluconate than any other hexonic acids tested in substrates. $K_m$ and $V_{max}$ values of rSso_DHAD were calculated as $0.54\;{\pm}\;0.04\;mM$ toward 2,3dihydroxyisovalerate and $2.42\;{\pm}\;0.19\;mM$ toward D-gluconate, and as $21.6\;{\pm}\;0.4\;U/mg$ toward 2,3-dihydroxyisovalerate and $13.8\;{\pm}\;0.4\;U/mg$ toward D-gluconate, respectively. In the study for biochemical properties, the enzyme shows maximal activity between $70^{\circ}C$ and $80^{\circ}C$, and the pH range of pH 7.5 to 8.5. The half life time at $80^{\circ}C$ was 30 min. A divalent metal ion, $Mn^{2+}$, was only powerful activators, whereas other metal ions made the enzyme activity reduced. $Hg^{2+}$, organic mercury, and EDTA also strongly inhibited enzyme activities. Particularly, the rSso_DHAD activity was very stable under aerobic condition although the counterparts reported from mesophiles had been deactivated by oxygen.

• Journal of Life Science
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• v.16 no.2 s.75
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• pp.323-327
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• 2006

The Formation of acrylamide was studied in Maillard model reaction systems based on asparagine-glucose. The mixture of asparagine and glucose in equal molar ratio, and then heated at 125, 150, 175 and $200^{\circ}C$ for 10, 20 and 30 minute, respectively. The reaction products were extracted with ethyl acetate and methanol, and then isolated and detected on FFAP capillary column and HP-5MS 5% phenyl methyl siloxane column by using GC/MS. Acrylamide was detected only from methanol extracts and on FFAP capillary column, at retention time 23.53 min., and the detection limit was 4.6 ng. Acrylamide content mainly increased with increasing temperature and processing time till $175^{\circ}C$, therefore, maximal acrylamide formation occurred at $175^{\circ}C$ for 10 minute ($116{\mu}g/g$), while, above $175^{\circ}C$, higher temperatures or prolonged processing times caused a decrease of acrylamide levels, finally disappeared at $200^{\circ}C$ for 30 minute. Three major compounds were identified as 1,3-dihydroxypropanone, 2,3-dihydro-3,5-dihydroxy-6-methyl-4H-pyrane-4-one and 5-hydroxymethylfurfural, and three minor compounds also as 5-methylfurfural, 2-acetylpyrrole and N,N-dimethylcyclohexamine, from ethyl acetate or methanol extracts on FFAP or HP-5MS capillary column.

• KSBB Journal
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• v.23 no.5
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• pp.452-459
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• 2008

To obtain maximal efficacy with minimal systemic side-effects, many studies have been carried out to achieve the controlled release of 5-fluorouracil (5-FU). In this study, biodegradable poly(L-lactide) (L-PLA) microparticles containing 5-FU were prepared by a process, called aerosol solvent extraction system (ASES), utilizing supercritical carbon dioxide. The effects of various organic solvents, drug/polymer feeding ratio, polymer molecular weight, and blending with the same polymers with different molecular weights on the formation of 5-FU loaded microparticles were investigated under a predetermined operating condition from our previous study. The drug recovery, entrapment efficiency, and in vitro drug release kinetics were determined by HPLC assays. The drug recovery obtained from the ASES process was found to be very high, whereas the drug entrapment efficiency was considerably low in all the experiments due to the poor affinity between L-PLA and 5-FU. These results indicated that the precipitation rate of L-PLA might be quite different from that of 5-FU so that there was little chance to form 5-FU loaded L-PLA microparticles.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.14 no.1
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• pp.77-88
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• 2011

This study was carried out to investigate the effects of soil depth and planting density on the growth of lettuce, crown daisy, and strawberry on a rooftop condition using artificial soil as a growth media. The vegetable crops showed better growth for plant height (cm), plant width (cm), plant fresh weight (g), and Fo, Fm and Fv/m on 20cm depth soil than 10cm depth soil except strawberry. Planting density of $16/m^2$ and $64/m^2$ did not show significant differences on the growth of the crops. Soil moisture content and EC were low for 10cm depth soil in lettuce plots, whereas there was no significant differences on soil moisture and EC between two soil depth in strawberry plots. Hunter's L, a, and b values showed the leaf color of lettuce dark green on 20cm depth soil and reddish on 10cm depth soil. Results showed that soil depth suitable for crop growth on rooftop conditions was 20cm rather than 10cm. Growth response of the crops showed no significant difference between $16/m^2$ and $64/m^2$, indicating that planting density of 64 $plants/m^2$ could be practiced on rooftop conditions. Lettuce growth rapidly changed in control treatment in which leaves were not pinched out, while slowly changed in plants which leaves were periodically pinched out. In the case of control plot, it was impossible to harvest because withering of lower leaves after blossom on June 22. The plant of crown daisy in which pinching was not conducted, blossomed on June 7, and the plants were removed since its aesthetical value was lost. Strawberry seemed to be a suitable vegetable crop for rooftop conditions based on its high covering rate and extended growth period until late October. The soil depth 20cm and planting density 64 $plants/m^2$ were suitable for vegetable crops on green roof system using artificial soil.

• Applied Chemistry for Engineering
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• v.10 no.1
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• pp.154-159
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• 1999

EDTA (ethylenediaminetetraacetic acid), a chelating agent is most widely used in industrial applications, especially for cleaning of metals in water, frequently prohibits metal removal from water in conventional water treatment technologies. It could be easier to remove aqueous metal ions by the breakdown of DETA complexed bonds first. This study investigated the availability of $TiO_2$ photo-catalysis for the aqueous phase oxidation of Cu(II)-EDTA, under an aerobic condition at $20^{\circ}C$ with $TiO_2$ (Degussa P-25) and 1.79mM of Cu(II)-EDTA. When $TiO_2$ loading was 2.0 g/L, the photo-catalytic oxidation of Cu(II)-EDTA was maximal. The tendency of EDTA adsorption onto the catalyst surface was affected by $TiO_2$ surface charge, and the oxidation rate of Cu(II)-EDTA by photo-catalysis was shown to be dependent upon the tendency of EDTA adsorption before photo-irradiation.

• Journal of the Korean Vacuum Society
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• v.22 no.3
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• pp.168-173
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• 2013

In this paper, we would like to introduce Electron Relay Enhancer (ERE), a supplementary device, which improves commercial solar cell efficiency minimizing electron-hole recombination of solar cell. The ERE in this study is mainly composed of two capacitors which are connected to AC power source and bridge diode system which controls electron flow direction. Two capacitors repeat collecting electrons from solar cell and pumping the collected electrons to load resistance or inverter through the bridge diode system. While one positively charged capacitor collect electrons, the other negatively charged one pumps electrons. A positively charged capacitor pulls the more exited electrons from the solar cell, before the exited electrons recombine the holes in solar cell. That is why the ERE system enhances solar cell efficiency. As a result, the measured power increase of the solar cell with the ERE is varied from 5.9 W to 25.6 W in each experimental condition. Maximal increase rate of the solar cell power with ERE is 30.8% of solar cell power without ERE.