• 한국미생물생명공학회:학술대회논문집
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• 한국미생물생명공학회 2001년도 Proceedings of 2001 International Symposium
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• pp.163-164
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• 2001

A number of bacterial species produce extracellular lipases. Among them, many lipase genes have been cloned and sequenced. A comparison of primary sequences revealed only very limited sequence homology among them. Based on the sequence homologies and molecular sizes (Mr), bacterial lipases were classified into four discrete groups. From soil samples taken around Taejon, five different lipase-producing bacteria were isolated; Proteus vulgaris K80, Bacillus stearothermophilus Ll, B. pumilus B26, Staphylococcus haemolyticus L62, S. aureus B56. Nucleotide sequence analysis showed that Staphylococcus lipase genes (L62 and B56) composed of pre-pro-mature parts, Bacillus lipase genes (Ll and B26) pre-mature parts, and Proteus lipase gene (K80) mature part only. In addition, the molecular sizes of their mature parts were quite different from 19,000 to 45,000. Finally, they had very little homology (less than 20％) in their amino acid sequences. Judging from the above results, lipase K80 belonged to bacterial lipase Group I, lipase L1 and lipase B26 Group III, and lipase L62 and lipase B56 Group IV. This diversity in their primary structures was also reflected in their enzymatic properties; temperature effects, pH effects, substrate specificity, detergent effects, and so on.

• Journal of Plant Biotechnology
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• 제29권2호
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• pp.117-121
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• 2002

히어리나무 1년생 및 10년생을 재료로 액아배양을 통한 기내번식을 시험하였다. 줄기의 증식은 zeatin과 BA의 공조처리가 효과적이었으며 MS배지에 zeatin 0.5∼3.0 mg/L, 0.2 mg/L BA 처리시 주효하였다. 1년생이 10년생보다 전반적으로 증식 및 생장이 양호하였으며, 배양 6개월 후에는 10년생에서도 매월 절편 당 3개의 줄기유도가 가능하였다. 기내 줄기의 발근은 1년생은 97%, 10년생은 62%를 나타내었고, 토양이식시 1 년생 유래 배양묘는 67%, 10년생은 48%생존되어 모수령에 따른 차이를 나타냈다. 본 실험결과 히어리나무의 액아배양으로 유시, 성숙목의 대량번식이 가능함을 보여주었으나 선발개체의 효율적인 기내번식을 위해서는 재유령화의 기술개발과 토양순화율을 증진시켜야 하는 것으로 나타났다.

• 한국연초학회지
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• 제2권1호
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• pp.37-52
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• 1980

신개간지 토양에 대한 인산시용 수준별 석회, 퇴비증시, 퇴비와 붕소의 혼합시용에 따른 비옥도 증진의 효과가 연초의 수량 및 품질에 미치는 영향을 2년간에 걸쳐 시험한 결과를요약하면 다음과 같다. 1. 개량제 처리의 효과는 1차년도에서 보다 2차년도에 담배 생육과 엽면적지수와 단위엽 면적중을 월등하게 증가시켰다. 3. 개량제의 시용으로 초년도의 pH, 치환성염기등이 숙전과 같은 수준으로 개선되었으며, 처리별 비옥도 증진효과는 퇴비증시구가 컸다. 3. 2차년도 시험결과에서 수량은 대조구에 비하여 15∼25% 증수되었고, 품질은 처리간에 큰 차 없으나, 개량제 시용으로 대조구에 비하여 3 - 7% 향상되었다. 수익 (10a당 수납대금)은 인산흡수계수에 대한 3 %조절량+퇴비증시구가 가장 높아 대조구보다 34%의 증가를 가져왔다.

• 한국토양비료학회지
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• 제49권2호
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• pp.208-217
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• 2016

The Rural Development Administration moved to Jeonju for the balanced development of the land. This situation required establishment of new research fields with soils appropriate to cultivation. We applied a variety of amelioration techniques to mature soils of new research fields of the National Institute of Agricultural Science (NAS) and evaluated effects of the integrated amelioration techniques. The schedule of amelioration was following: 1) location of research fields was determined, 2) surface and subsoil samples were collected separately, 3) after aligning the top level of research fields, subsoil and surface soil were re-established with soil amendment, 4) the green manure crops were grown four seasons to improve the uniformity and increase the organic content of the research field, and 5) drainage canal and/or underdrainage were applied to poorly drained fields. The last green manure crop was rape in RDA fields and green barley in NAS fields. The average height, fresh weight, and dry matter weight of rape in good condition were 123 cm, $3,938kg\;10a^{-1}$, and $651kg\;10a^{-1}$, respectively. The height, fresh weight, and dry matter of green barley, on average, were 97 cm, $3,013kg\;10a^{-1}$, and $1,004kg\;10a^{-1}$, respectively. In the chemical properties of paddy field, pH and levels of silicate, calcium, magnesium, and potassium were in appropriate range but organic matter content of $16g\;kg^{-1}$ was less than the optimum level. In the chemical properties of upland field, pH and levels of phosphorus, calcium, magnesium, and potassium were appropriate range but organic matter content of $12g\;kg^{-1}$ was less than the optimum range. Evaluation of well-adapted soil was performed. The field in RDA was classified into the superior class with points ranging from 90 to 95 by the field evaluation test. The fields in NAS were mainly evaluated as the superior class with points greater than 85. However, some fields in NAS remained low quality with scores between 80 and 83. Further soil amelioration practices were suggested to fields with low soil quality.

• Journal of Plant Biology
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• 제37권3호
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• pp.333-341
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• 1994

Effective plant regeneration from immature cotyledons of soybean [Glycine max (L.) Merr.] cultivars was achieved via somatic embryogenesis. Somatic embryogenesis was performed with the cotyledons of immature embryos 14-20 d after flowering. Immature cotyledons of cv. Whangkeum were placed abaxial or adaxial side down on modified MS medium containing 20mg/L 2,4-D. The greatest number of somatic embryos, 1.2 per cotyledon, was produced from those of 4.0-4.9 mm in length which had been placed abaxial side down. Among cvs. Pecking, Whangkeum and Baekwoon, Pecking had the highest embryo induction efficiency with 4.3 somatic embryos per cotyledon in 20mg/L 2,4-D treatment and with 1.0 embryo per cotyledon in 8mg/L NAA treatment. Germinable globular somatic embryos were induced with the highest efficiency, 27.6%, in 20mg/L 2,4-D and were proliferated efficiently on liquid medium containing 10mg/L 2,4-D. The globular somatic embryos developed into germinable mature somatic embryos on medium containing 10 $\mu$M CoCl2, 9% sucrose, and 0.5% activated charcoal. These mature somatic embryos germinated on hormone-free mediu. After transfer to the soil, regenerated plants with seeds were obtained.

• 한국조경학회지
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• 제26권2호
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• pp.166-180
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• 1998

In a city where the greenery always lacks landscaping roofs of existing buildings offers a way to solve the problem. But the most serious problem that poses is the matter of load limit. At present most existing buildings have the loading capacity of 200 kg/$m^2$ or less on their roofs. If the natural soil is used the loading capacity is easily surpassed because it simply is too heavy. To alleviate this problem it is realized to introduce the light weight artificial soil. The specific gravity of light soil is 0.65 whereas the natural soil is 1.8 when wet. It is three times lighter than the natural soil, thus eases the burden to the roof. The next problem to be confronted is the plant species to plant. It is possible to plant trees but they soon outgrow the loading capacity by weighing 8 times heavier in 10 years. Therefore shrubs and perennials are suggested to be planted because they don't weigh much even when they reach the mature height. The last problem is the stress put on roofs by the weight of the users. By some unexpected event the crowd gathered on a weak roof can cause the structural damage or even the collapse of the roof. The avoid the possibility of collapse a plaza or big pocket should not be designed in a roof garden because they hold crowd. By following the suggested means the old roofs of existing buildings can turn into the urban oases in the sky.

• 한국산림과학회지
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• 제86권3호
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• pp.391-398
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• 1997

참나무류의 효율적인 기내 번식법 개발을 위하여 상수리나무 유묘와 수형목을 재료로 액아를 배양하였다. 5가지 배지 가운데 WPM 배지에서 줄기 분화 및 생장이 가장 양호하였다. 싸이토키닌중 zeatin 3.0mg/L의 농도에서 줄기 분화 및 생장에 효과를 보였으나 대체로 BAP의 처리가 다경 줄기 분화 및 생장에 주효하였다. BAP와 zeatin의 공조처리는 다경줄기 유도 및 생장에 더욱 효과가 있었으며, 적정 농도는 BAP 0.5, zeatin은 0.05-1.0mg/L이었다. 기내발근은 1/2 GD 배지에 0.5mg/L IBA 처리로 90% 이상 발근되었고 인공 배양토에서 70% 이상 활착되었다. 유령 대목에 2회 접목된 27개 수형목 클론을 재료로 시험한 결과는 4개 클론만이 기내 증식의 가능성을 보였다. 재유령화된 수형목의 기내 번식은 모수령의 영향보다는 클론간의 차이에 크게 좌우되었다. 기내 반응이 우수한 수형목 클론은 증식후 90% 이상 발근되었다. 발근배지는 염류를 반감시킨 GD배지에 IBA 0.2-2.0mg/L 처리시 주효하였다. 발근묘는 인공 배양토에 이식하여 5개월 후 60% 이상 활착되었다.

• Nuclear Engineering and Technology
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• 제24권2호
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• pp.121-129
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• 1992

pH 6.35치 사질양토에 g당 5.2 및 31.2Bq의 Sr-90을 처리하여 벼와 콩에 의한 Sr-90 흡수 실험을 pot재배를 통하여 실시하였다. 두 작물에 의한 Sr-90흡수율은 성숙기까지 계속 증가하였고 성숙기에 흡수율은 공히 1.0% 정도였다. 그러나 전지상부내 농도는 성장과 함께 계속 감소하거나 거의 변하지 않았다. 두 작물의 부위별 Sr-90농도는 처리농도의 증가에 따라 증가하였다. 성숙기에 Sr-90의 토양작물체간 전이계수는 건조중 기준으로 벼의 경우 0.07(현미) -3.67(잎), 콩의 경우 0.86(종실) -9.26(잎)의 분포를 보였다. 현미에 있어서는 전이계수가 5.2Bq 처리에서는 0.17, 31.2Bq 처리에서는 0.07로 처리농도간에 유의차가 인정되었다. 작물 수확후 상부 15cm 토양내 Sr-90의 잔류율은 약 80％였다 Sr-90홉수는 두 작물의 생육이나 수량에 영향을 미치지 않았다.

• 한국토양비료학회지
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• 제49권6호
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• pp.644-650
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• 2016

In soil-to-plant transfer of heavy metals, the amount absorbed and accumulated varies depending on the environment conditions. The absorption rate of cadmium (Cd) in plants differs considerably depending on the bioavailability of Cd in the soil, while usage by various organic matters is also reported to affect absorption patterns. Therefore, this study aimed to identify the difference in the transfer of essential metal ions and Cd to various plant parts when rice straw compost was used to cultivate soybean (Glycine max L. cv. Daepung). In the two-leaf stage of soybean cultivated in a greenhouse, Cd was mixed in the soil, after which the Cd and essential metal ions contents, and physiological changes of soybean seedlings were studied on the 15th and 25th day. The Cd toxicity in the plant was reduced with the use of rice straw compost. Further, the Cd content varied with the plant part, and was higher in young leaves (3rd and 4th leaf) than in the stem. When analyzed by leaf age, the Cd transfer was highest in young leaves (3rd and 4th leaf), followed by mature leaves (1st and 2nd leaf). While there was no significant difference between plant tissues in the absorption rate of copper (Cu) and zinc (Zn) when rice straw compost was used against Cd toxicity, the absorption rate of manganese (Mn) and iron (Fe) showed a significant decline in both the control and rice straw compost treatment conditions, as well as a significant difference between leaf ages. Therefore, these results confirm that the use of rice straw compost against Cd toxicity is effective, and implies that the rate of Cd transfer in the soybean plant varies significantly with leaf age.

• Journal of Plant Biotechnology
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• 제33권4호
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• pp.257-262
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• 2006

The paper evaluated the behavior of in vitro culture responses from a diverse set of Indica rice (Oryza sativa L.) genotypes. Significant differences were found in embryogenic callus induction frequency, callus growth and plant regeneration frequency when mature embryos of 11 cultivars, breeding lines and land races were compared. Genotype as well as plant growth regulator influenced the plant regeneration frequency. Callus induction frequency was not correlated with callus growth as well as plant regeneration frequency. The regenerated plants could grow to normal, fertile plants after they were successfully established in soil.