• Title/Summary/Keyword: mature nodal segment

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Shoot Regeneration of Mature Nodal Segments in Poplar (Populus deltoides) (미류나무 성숙 절간조직으로부터 식물체 증식)

  • Yang Hee-Sun;Kang Hoduck
    • Korean Journal of Plant Resources
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    • v.18 no.3
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    • pp.403-409
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    • 2005
  • Mature nodal segments of 2-year-old greenhouse stock plant were cultured on Murashige and Skoog basal medium supplemented with the different kinds and various concentrations of cytokinins to produce multiple shoots in in vitro condition. The most adventitious shoots were produced from excised ends of nodal segments. The highest average number $(24.6\;{\pm}\;4.6)$ of shoots was produced with the combination of BA 1.0mg/L and TDZ 0.1mg/L in MS medium. In addition, several shoots were formed from lenticels of bark cambium with the same treatment. These concentrations promoted high shooting capability upto $94.6\%$ and NAA was the best cytokinin among five different PGR sources.