• Title/Summary/Keyword: mating design

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Analysis of Mating System in Lentinula edodes and Development of Mating Type-Specific Markers

  • Ha, Byung-Suk;Kim, Sinil;Ro, Hyeon-Su
    • 한국균학회소식:학술대회논문집
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    • 2014.10a
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    • pp.42-42
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    • 2014
  • Mating of tetrapolar mushrooms is regulated by to chromosomal loci, A and B. A locus contains A gene that expresses a homeodomain protein whereas B locus contains multiple pheromones and receptor genes. In order to characterize the mating loci in Korean cultivated strains of Lentinula edodes, one hundred monokaryotic myclelia were isolated from the basidiospores of cultivated strains, including Cham-A-Ram, Sanjo701, and Sanjo707. Both mating loci were amplified using primer sets targeting conserved sequence regions for homeodomain (HD), pheromone, and receptor genes. Subsequent sequence analysis revealed that the Korean strains contained significant variations in the homeodomain of A locus, even within the same A1 or A2 mating type. Similarly, B locus was also highly diversified in the sequences of pheromones and receptors as well as gene organization. These results enabled us to design mating type-specific probes which can distinguish mating type of each strain. The specificity was confirmed by between intra- and inter-strain mating experiment.

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LMU Design Optimization for the Float-Over Installation of Floating Offshore Platforms (부유식 해양구조물의 플로트오버 설치용 LMU 최적설계)

  • Kim, Hyun-Seok;Park, Byoungjae;Sung, Hong Gun;Lee, Kangsu
    • Journal of the Computational Structural Engineering Institute of Korea
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    • v.34 no.1
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    • pp.43-50
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    • 2021
  • A Leg Mating Unit (LMU) is a device utilized during the float-over installation of offshore structures that include hyperelastic pads and mating part. The hyperelastic pads absorb the loads, whereas the mating part works as guidance between topside and supporting structures during the mating sequence of float-over installation. In this study, the design optimization of an LMU for the float-over installation of floating-type offshore structures is conducted to enhance the performance and to satisfy the requirements defined by classification society regulations. The initial dimensions of the LMU are referred to the dimensions of those used in fixed-type float-over installation because only the location and the number of LMUs are known. The two-parameter Mooney-Rivlin model is adopted to describe the hyperelastic pads under given material parameters. Geometric variables, such as the thickness, height, and width of members, as well as configuration variables, such as the angle and number of members, are defined as design variables and are parameterized. A sampling-based design sensitivity analysis based on latin hypercube sampling method is performed to filter the important design variables. The design optimization problem is formulated to minimize the total mass of the LMU under maximum von Mises stress and reaction force constraints.

Contact Stress Analysis of a Pair of Mating Spur Gears (스퍼기어의 접촉응력 해석)

  • Lee, Jin-Hwan;Lee, Dong-Hyong;Lee, Kwon-Hee
    • Journal of the Korean Society of Manufacturing Process Engineers
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    • v.9 no.4
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    • pp.59-65
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    • 2010
  • This paper presents the study on the contact stress analysis of a pair of mating spur gears during rotation. Contact stress analysis is performed between two spur gear teeth at different contact positions during rotation. An example is presented to investigate the variation of contact stress on a pair of mating gears with contact positions. The variation of contact stress during rotation is compared with the contact stress at lowest point of single tooth contact(LPSTC) and AGMA Equation for contact stress. The results show that contact stress varies along the contact position and gets maximum values in the beginning and end of the contact. In this study, the gear design considering the contact stress on a pair of mating gears is more severe than that of AGMA standard.

Design of a new omnidirectional image sensing system for assembly (OISSA) (조립을 위한 새로운 전방향 시각장치의 설계)

  • Kim, Wan-Su;Cho, Hyeong-Seok;Kim, Seong-Gwon
    • Journal of Institute of Control, Robotics and Systems
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    • v.4 no.1
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    • pp.88-99
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    • 1998
  • In assembly, misalignment must be detected and compensated for during the mating period, regardless of the complexity of the cross-sectional shape. To this end, we propose a novel omnidirectional image sensing system for assembling parts with complicated shapes(OISSA) and its feasibility for detecting the misalignment between mating parts is shown by a series of simulations. This system encompasses a camera with an optical unit attached to the front of the camera. The optical unit consists of a pair of plane mirrors and a pair of conic mirrors. Utilizing the proposed sensing system, a 2$\pi$ coaxial image of the misalignment along the mating boundary interface between mating parts can be immediately obtained without experiencing self-occlusion.

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Analysis of Cone and Seed Characteristics from Different Mating Design Strategies of Pinus densiflora for. multicaulis

  • Lee, Kyungmi;Lee, Hyunseok;Kang, Jun-Won
    • Journal of Forest and Environmental Science
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    • v.37 no.4
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    • pp.309-314
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    • 2021
  • This study aimed to enhance seed productivity and secure genetic resources for Pinus densiflora for. multicaulis. We analyzed the characteristics of cone and seed generated by control pollination between Pinus densiflora (PD) and Pinus densiflora for. multicaulis (PDM). The highest number of cone scales (63.0) was obtained from the self-pollinated (sp) PDM clone B (PDM-sp-B), whereas the lowest number of cone scales (44.7) was obtained from two combinations designated as PDM-A×PD-075 and PDM-A×PD-0111. Both female parents of the hybrids were PDM-A. The highest seed production capacity (80.8) was obtained from the open-pollinated (op) PDM clone B (PDM-op-B). The seed potentials of PDM-B×PD-0111, PDM-op-A, and PDM-sp-B were 67.4, 66.5, and 63.1, respectively. The highest number of fertile scales (41.5) was obtained from PDM-op-B, and the lowest number of fertile scales (28.8) was obtained from PDM-A×PD-075. The total number of aborted ovules and 1st aborted ovules was not statistically significant in the mating design. The cross combination of PDM-B×PD-0111 had the highest number (34.8) of filled seeds and the lowest number of 2nd aborted ovules (5.2) and empty seeds (9). PDM-op-B had the highest number of developed seeds (47.6), although the number of empty seeds was the highest (41.2). Therefore, we conclude that the mating design of PDM-B×PD-0111 is useful for future breeding programs to improve seed yield of PDM. Our results showed that there was a strong correlation between the following two parameter pairs: number of scales and number of fertile scales, and the number of fertility scales and seeds potential (r=0.89 and r=0.84, respectively; both p<0.01).

Effect of Strategic Feed Supplementation during Gestation on Intake, Blood-biochemical Profile and Reproductive Performance of Goats

  • Rastogi, Ankur;Dutta, Narayan;Sharma, K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.16 no.12
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    • pp.1725-1731
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    • 2003
  • Effect of strategically supplemented pregnancy allowance was ascertained during different phases of gestation on the reproductive performance of does. Gravid does (18) were allotted to 3 dietary treatments HH, HL and LH in a completely randomized block design. All does were provided wheat straw ad libitum and supplemented with concentrate mixture at the rate of $20g/kgW^{0.75}$ from 0-60 days post-mating. Subsequently, HH group was given concentrate at the rate of $40g/kgW^{0.75}$ from 61 d post-mating to term. HL group was offered concentrate from 61-90 d post-mating and 121d post mating to term at the rate of $20g/kgW^{0.75}$ and from 91-120 d post-mating at the rate of $40g/kgW^{0.75}$. LH group was provided with concentrate from 61 to 120 d post-mating and 121 to term at the rate of 20 g and $40g/kgW^{0.75}$, respectively. Mean total dry matter and concentrate intake ($g/kgW^{0.75}$) was significantly higher on HH in comparison to comparable intake of HL and LH treatments, however, wheat straw followed the reverse trend. Haematological and biochemical parameters except serum glucose, total serum protein and A:G ratio did not differ significantly among dietary treatments. The gross gain in weight, products of pregnancy at 21 weeks of gestation, pregnant does average daily gain, birth weight of kids and survivability of kids were significantly lower in treatment HL in comparison to comparable values for HH and LH treatments. The net gain in gravid does was significantly higher in treatment HH than the comparable values obtained in HL and LH treatments. The last month of pregnancy was found to be nutritionally most sensitive period of gestation for native goats.

Design and Implementation of the genome-level fragment assembly system, Mater (Fragment Assembly를 위한 시스템의 설계 및 구현)

  • 김명선;정철희;박현석
    • Proceedings of the Korean Information Science Society Conference
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    • 2001.04a
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    • pp.751-753
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    • 2001
  • 지금까지 인간이나 다른 생물체의 전체 유전체 염기서열을 밝혀내는 작업은 크게 세가지 방법으로 진행되었다. Clone-by-clone approach, sequence tagged connector approach, random shotgun approach[1]가 그것인데 마지막의 random shotgun approach는 fragment assembly problem을 비롯한 여러 가지 전산학적인 문제들을 수반한다. 미생물체의 전체 염기서열을 random shotgun approach를 이용하여 밝혀낼 때 몇 가지 전산학적인 문제가 테크닉이 필요하며 그 중에서도 서열간의 forward, reverse의 mating 정보를 이용하는 것이 중요하다. 본 논문은 이러한 mating 작업을 한 눈에 볼 수 있게 하는 소프트웨어 페키지 “Mater”에 대해 소개하고자 하며 그 의미에 대해 논하고자 한다.

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Representation of Spatial Relationships for Sheet Metal Weld Assemblies Modeling (박판 용접구조물의 모델링을 위한 공간관계 표현)

  • 김동원;김경윤
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 1997.04a
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    • pp.400-404
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    • 1997
  • This paper presents spatial relationshaps and engineering features for the feature based modeling of Sheet Metal Weld Assemblies (SMWA) that are made of sheet metal components through are welding processes. Spatial relationships in ProMod-S, a sheet metal product modeler,are further extended for the SMWA modeling. Some spatial relationships for special weld joint types are newly introduced. The geometrical and topological relations between spatial reationship, mating features, and assembly features are defined. Finally, assembly data stucturess for the product modeling of SMWA are proposed. They are an engineering relation to represent the constraints between component features, and a mating bond to integrate component design information.

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Contact Stress Analysis of Helical Gear for Turbo Blower (터보블로워용 헬리컬 기어의 접촉응력 해석)

  • Hwang, Seok-Cheol;Lee, Dong-Hyong;Park, Young-Chul;Lee, Kwon-Hee
    • Journal of the Korean Society of Manufacturing Process Engineers
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    • v.10 no.2
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    • pp.90-95
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    • 2011
  • This paper presents the study on the contact stress analysis of a pair of mating helical gears for turbo blower during rotation. Turbo blowers need high speed rotation of impeller in structure and high rate gear ratio. The use of helical gear indicated that noise was an important problem when the application involves high speeds and large power transmission. An example is presented to investigate the variation of contact stress on a pair of mating gears with contact positions. The variation of contact stress during rotation is compared with the contact stress at the lowest point of single tooth contact(LPSTC) and AGMA Equation for contact stress. In this study, the gear design considering the contact stress on a pair of mating gear is more severe than that of AGMA standard.

Evaluation of Reciprocal Cross Design on Detection and Characterization of Non-Mendelian QTL in $F_2$ Outbred Populations: I. Parent-of-origin Effect

  • Lee, Yun-Mi;Lee, Ji-Hong;Kim, Jong-Joo
    • Asian-Australasian Journal of Animal Sciences
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    • v.20 no.12
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    • pp.1805-1811
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    • 2007
  • A simulation study was conducted to evaluate the effect of reciprocal cross on the detection and characterization of parent-of-origin (POE) QTL in $F_2$ QTL populations. Data were simulated under two different mating designs. In the one-way cross design, six $F_0$ grand sires of one breed and 30 $F_0$ grand dams of another breed generated 10 $F_1$ offspring per dam. Sixteen $F_1$ sires and 64 $F_1$ dams were randomly chosen to produce a total of 640 $F_2$ offspring. In the reciprocal design, three $F_0$ grand sires of A breed and 15 $F_0$ grand dams of B breed were mated to generate 10 $F_1$ offspring per dam. Eight $F_1$ sires and 32 $F_1$ dams were randomly chosen to produce 10 $F_2$ offspring per $F_1$ dam, totaling 320 $F_2$ offspring. Another mating set comprised three $F_0$ grand sires of B breed and 15 $F_0$ grand dams of A breed to produce the same number of $F_1$ and $F_2$ offspring. A chromosome of 100 cM was simulated with large, medium or small QTL with fixed or different allele frequencies in parental breeds. A series of tests between Mendelian and POE models were applied to characterize QTL as Mendelian, paternal, maternal or partial expression QTL. The overall detection powers were similar between the two mating designs. However, the proportions of paternally expressed QTL that were declared as paternal QTL type were greater in the reciprocal cross design than in the one-way cross, and vice versa for Mendelian QTL. When QTL alleles were segregating in parental breeds, a significant proportion of Mendelian QTL were spuriously declared POE QTL, suggesting that care must be taken to characterize imprinting QTL in a QTL mapping population with a small number of $F_1$ parents.