흰쥐 침샘의 방사선조사시 Apoptosis에 대한 Diltiazem과 Pentoxifylline의 효과 (The Effects of Diltiazem and Pentoxifylline on Apoptosis of Irradiated Rat Salivary Gland)
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- Radiation Oncology Journal
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- 제16권4호
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- pp.367-375
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- 1998
목적 : 두경부에 방사선 조사를 받는 경우 대부분의 환자에서 구강 건조증을 겪게 된다. 이와 같은 구강 건조증은 침샘 세포의 소실에 의해 침이 생성되지 않거나 적게 생성되기 때문이다. 침샘 세포가 방사선 조사에 의해 소실되는 기전은 침샘 세포의 간기사망(interphase death) 혹은 apoptosis에 의한 직접 손상에 의해 발생된다는 것이 가장 가능성 있는 기전으로 알려져 있고 apoptosis는 세포내의 칼슘농도의 증가로 인해 일어난다. 따라서 본 연구는 흰쥐 침샘의 방사선조사에서 칼슘통로 차단제인 diltiazem과 방사선의 급성반응에 작용하고세포 내 칼슘농도를 감소시킬 수 있는 pentoxifylline을 방사선조사 전에 투여하여 apoptosis 발생이 감소되는 지를 알아보고자 하였다. 대상 및 방법 : 체중 약 200-250g의 흰쥐 10마리를 대상으로 대조군, 방사선조사 단독군, 방사선 조사전 diltiazem 혹은 pentoxifylline을 투여군과 두 약제를 동시에 동시 투여군으로 나누었다. 방사선 조사는 침샘을 포함하는 두경부에 1600cGy를 단일 조사하고 diltiazem은 쥐의 체중 kg 당 30mg을 방사선조사 30분전, pentoxifylline은 쥐의 체중 kg 당 50mg을 방사선조사 20 분전에 복강내 투여하였다. 방사선조사 후 24시간에 쥐를 회생시켜 이하선과 악하선을 제거하여 hematoxylin-eosin 염색을 한 후 200배의 배율에서 무작위로 10-20곳을 선택하여 현미경시야당 전체 세포수(T)에 대한 apoptotic body의 수(A)의 백분율(A/TX100)을 구하여 각 군을 비교하였다. 결과 : 이하선에서 방사선단독, diltiazem, pentoxifylline 단독 투여군과 두약제의 동시 투여군에서 관찰된 apoptosis 백분율은 각각 1.72
본 연구의 목적은 고등학교 교육과정에서 활용할 수 있는 가정교과 기반의 예비부모교육 프로그램을 개발하고 평가하여, 예비부모인 고등학생 학습자들이 훗날 우리 사회의 주역이 될 미래세대를 현명하게 보살필 수 있는 성숙한 부모가 되는데 필요한 역량을 갖추도록 하며 동시에 현재 정부가 추진하고 있는 부모교육 활성화 방안의 청소년 대상 예비부모교육을 실행하는 데 도움이 되는 기초자료를 제공하는데 있다. 본 연구의 예비부모교육프로그램 개발 및 평가 과정은 일반적체제설계모형인 ADDIE 모형을 따랐으며, ADDIE모형 중 실행단계(Implement)를 제외한 분석, 설계, 개발, 평가의 4가지 과정을 거쳐 이루어졌다. 먼저, 분석 단계에서는 프로그램 내용요소 추출과 프로그램 개발의 시사점을 얻기 위해 관련 선행연구와 2015 개정 중 고등학교 "기술 가정" 교육과정 문서를 대상으로 분석이 이루어졌다. 그 결과 가정교과 이 외의 분야에서 연구되어 온 예비부모교육의 9 가지 주제(1.자기이해, 2.예비부모의 성, 3.결혼, 4.임신과 출산, 5.부모됨, 6.부모역할, 7.부모 자녀관계, 8.부모와 가족, 9.부모와 사회)와 해당 내용요소들은 이미 가정교과 교육과정에 모두 포함되어 있음을 확인하였으며, 9가지 주제 이외에 가정교과만의 독자적인 주제(1.생애설계, 2.가정생활 내 의 식 주 실천역량, 3.가정생활과 자녀안전, 4.가족문화)와 해당 내용요소들을 별도로 확인함으로써 예비부모교육 시행에 있어 가정교과가 지닌 강점(청소년과 가정과 사회를 바라보며 부모교육 내용 뿐만 아니라 식 의 주 소비 생활 전반을 포함하는 총체적 관점의 교육과 실제로 부모 준비를 실천하게 하는 교육)을 확인하였다. 둘째, 프로그램 개발을 위한 설계는 가정교과와 예비부모교육 간의 접점을 찾고 이를 프로그램 구성요소(목적, 개발방향, 주제, 내용요소, 학습목표, 학습활동)에 반영하는 과정을 통해 이루어졌다. 이 연구의 예비부모교육 프로그램은 미래세대를 현명하게 길러낼 수 있는 성숙한 부모가 지녀야 할 관련 지식, 태도, 가치관, 실천적 문제 해결 능력을 고등학생 학습자가 갖추도록 지원하는 것을 일차 목적으로 두며, 부모됨의 준비 관점에서 학습자가 현재 생활 점검 및 개선을 통해 생활자립능력을 갖추도록 하는 것을 이차목적으로 둔다. 궁극적으로 이 두 가지 목적 달성을 통해 학습자가 장차 부모로서 살아갈 가정생활 영역과 개인적 성취와 관련된 직업생활 영역 그리고 사회 발전에 기여할 수 있는 민주시민으로서의 삶의 영역간의 균형을 이뤄 이를 조화롭게 영위할 수 있는 성숙한 부모로의 성장을 돕고자 하였다. 프로그램 개발방향은 '전체 전개', '내용구성', '교수 학습법 구성'으로 크게 세 가지 측면에서 설정되었다. 프로그램의 주제는 총 11개로 '1. 부모 됨: 부모가 된다는 건', '2. 배우자선택: 행복한 부부관계, 자녀에게 주는 최고의 선물', '3. 임신과 출산: 새로운 생명과의 감동적인 만남', '4. 신생아 돌봄: 24시간 신생아 돌봄', '5. 영유아자녀돌봅: 사랑스러운 나의 아기와의 관계, 애착', '6. 유아기 자녀 돌봄: 별에서 온 내 아이, 유아기 자녀 돌보기', '7. 부모와 건강가정: 건강가정 속 부모와 자녀', '8.부모 자녀 관계: 현명한 부모, 자녀와 효과적으로 상호작용하기', '9. 가정생활 내 자녀안전: 가정생활 속 안전관리자, 부모', '10. 영유아 돌봄 실습', '11. 지역사회 양육지원서비스 개발 실습'이다. 프로그램의 들어갈 최종 내용요소들은 분석단계에서 추출된 내용요소들을 11개 주제와의 관련성을 토대로 분배된 후 선정되었다. 학습목표와 학습활동은 해당 주제와 내용요소를 반영하여 구상되었으며, 특히 프로그램의 학습활동은 1) 부모 됨 관련 실천적 문제를 포함한 사례 활용, 2) 학습한 지식과 기술을 활용한 지역사회 교류활동, 3) 부모 됨 관련 학습내용을 활용한 실생활 프로젝트 활동, 4) 고등학생 학습자의 현재 삶의 긍정적인 변화를 유도하는 활동, 그리고 5) 자녀 발달을 지원하는 가정교과 내 의 식 주 실습활동을 주요 특징으로 한다. 셋째, 프로그램의 개발은 앞서 설정된 설계에 따라 이루어졌으며, 이에 총 11개 주제에 따른 17차시 분량에 해당하는 교수 학습과 정안 및 학습자료가 개발되었다. 개발된 교수 학습과정안은 수업흐름 및 교사 참고자료를 포함하며, 수업 도입부에 가상의 자녀로부터 수업관련 메시지를 받는 것을 시작으로, 정리단계에서는 받은 메시지에 대한 답장의 형태로 해당 차시의 내용 정리 및 예비부모로서의 다짐을 하는 것을 기본 틀로 하였다. 학습자료는 학습활동을 위해 필요한 각종 계획서나 보고서 양식을 포함하며, 정규교육과정에서의 교과서와 같은 역할을 하도록 구체적으로 작성되었다. 넷째, 개발된 프로그램의 평가는 프로그램 개발과정과 결과물 두 가지 측면에 대해 가정교과 전문가 13인으로부터 5점 리커트형 설문지를 활용하여 이루어졌다. 개발과정에 대한 기초분석 평가결과 평균 4.61점, 내용타당도 지수 97.4%였으며, 프로그램 결과물에 대한 평가결과는 평균 4.37점 내용타당도 지수 86.9%였다. 이와 같은 값은 이 연구 프로그램의 개발과정과 그 결과물에 대한 타당도가 상당히 높은 수준에서 확보됐음을 나타내며, 이에 이 연구의 가정교과 기반의 예비부모교육 프로그램은 고등학생 학습자를 대상으로 하는 예비 부모 교육 프로그램으로 타당하고 적합하다는 결론을 내릴 수 있다.
Under the full consideration of the growing need and importance of population education in the field of home economics in Korea, the study was carried out to verify and assess the following facts on the current issues of population education of home economists who are presently engaging in teaching professions as the teachers of middle and high school and professors of college and universities by setting its primary objectives of the study as followings; 1) to assess the degree of general knowledge and attitudes of home economists toward population education in the field of home economics, 2) to verify the problems encountered in implementing population education by home economists in its field, 3) to find an existing status of previous trainings received and other activities of population education of home economists aimed at utilizing these findings as a part of reference materials when the population education is conducted in the field of home economics. In order to attain these objectives described above, the questionnaire was carefully designed to house a total of 40 questions with good combination of multiple-choice and the simple answer questions. The mail questionnaire survey was conducted by establishing teachers of home economics at middle/high schools and college/universities as Senior Home Economists(SHE) who are from public, private liberal arts and vocational schools. The rate of response observed during the survey was 45.6 percent and the findings of the survey research are as follows: 1) Examining the status of the respondents by residence and religion, it was found that 45 percent of middle & high school teachers ar.d 59. 1 percent of college professors are residing in Seoul city area and that the largest percent of them are christian in their religion. Analyzing respondents by their ages, 56 percent of middle/high school teachers are in their 30s, 45 percent of college professors are in their 40s, and 37 percent of college teachers are in their 30s. In addition, 13 percent of the total respondents are found to be unmarried. The study also revealed that 71 percent of the college professors finished Master Degree course and 82 percent of middle/high school teachers are graduated from college level lasting 4 years. Looking over the status cf major fields of respondents, 68.4 percent of middle/high school teachers are specialized in home economic education and the college professors, on the other hand, show relatively even prortion by specializing in the order of food & nutrition science, clothes & textile science and home managerial science. As far as the length of teaching experience is concerned, a relatively longer period of teaching experience is observed in the college professors in comparison with that of middle/high school teachers. In other words, 33.3 percent of middle/high school teachers are experienced in teaching from 6 to 10 years on average while 43.9 percent of college professors show more than 16 years of experience. 2) Examining the status of existing number of children cf the respondents, one boy and one daughter pattern is predominant, showing 28.5 percent in middle/high school teachers and 21.1 percent in college professors. As for the desired number of children of unmarried respondents, it is observed that 43.8 percent of middle/high school teachers desire to have one boy and one girl, and 31.3 percent of college professors want to have one child regardless of the sex. By assessing the degree of awareness of the population education through their students, it is observed that 53 percent of middle/high school teachers and 50 percent of college professors are aware of population education in some extent and that a majority of respondents took the positive attitudes toward an inclusion of family planning components into the formal school education. Another noteworthy to observe is that a total of 84.8 percent out of middle/high school teachers pointed that the population education currently conducted at schools as a part of home economics are less sufficient than it should be. 3) Analyzing the tendency as to whether the respondents were experienced in receiving population education during the time when they were students, 75 percent of college professors and 59 percent of middle/high school teachers responded negative answers in the survey. In the mean time, a total of 50 percent of the respondents replied that they began to acknowledge the importance of population education mainly through the participation of some sort of population-education orientend seminars, experienced by 40 percent of college professors and 80 percent of middle/high school teachers. 4) What it calls attention in this study was to find that 96.5 percent of middle/high school teachers and 72 percent of college professors conduct population education to some extent during their lecture hours and that more than 80 percent of them are never experienced in teaching population and family planning contents in their regular classes. It is, on the other hand, found that no more than once was the response of those who believe themselves that they are experienced in teaching these relevant components to their students. Analyzing the contents of the subjects being taught in the class, a large percent of them are found to be consisted of population and family planning contents. According to this study, the current population education through the formal school is quite inactive. Analyzing the facts, 44.9 percent of the college professors responded that the population and family planning components are quite apart from their specialization which eventually generates lack of interest in the field. 5) It is also noticed through the study that the degree of frequency of commenting on population and family planning contents during the classes was depending significantly on their specializations which means that the degree of frequency varies from a major to another. Those who majored in home managerial science was the first one, as compared to others who majored in different specializations. Glancing over the status of correlations between ages of the respondents and numbers of seminar paticipation, it is quite clear that the aged group participated more than the younger group did, and that the most highest number of participations made by college professors were those who are in 50s. In addition, it is also found that those who are aged 20s and 60s of the respondents were the group who comments least on the contents of population and family planning at their classes. The suggestions and recommendation made through this survey research are as follows. 1) No one denies that the rapid increase of population, as compared to the limited size of land and resources, will certainly affect adversly to an enhancement of individual life quality which will, eventually, bring forth the poverty of the nation. This is the reasson why we are insisting that the world population be controlled up to an optimum level with a matter of global concerns. It is our understading that the primary aim for reducing number of population is believed to be attained only by conducting the systematic and comprehensive population education through the formal schools. Therefore, the role of home economists in the field of population/family planning education is considered very importment due to the fact that an ultimate goal of population education is placed in elevating the quality of family life by having optimum number of children through family planning program. 2) It is quite clear that home economists as teachers of formal school in all level are invited to pay their attention on redefining the ultimate goal of education and that of population education. We also understant that the primary objective of population education is to change the norm and value of the clients by replenishing the students with pertinent knowledge and attitudes on population and its related problems through a sort of education in order to attain the ultimate goal for enhancing the quality of life. There is no exception in the theory of home economics. An altimate goal of home economics is to elevate the general quality of life through an establishment of value existed in daily life. Considering the relations between population education and home economics, it is quite indespensable to bandle population components as an integral part in the field of home economics. We believe, therefore, that the senior home economists positive participation in the effort population control is more needed than it has been. 3) It is also strongly urged that population education should be a part of instructor training course for home economics. In other words, the teacher of home economics should be well aware of population and its problems by teaching interrelationship between population education and home economics, needs, contents and methods of population education during the instructor training courese for home economics. In addition, the senior home economists should be encouraged through positive participation on the short term training by types of domestic and international seminar, workshop, etc. 4) We certainly believe that the population education can not sustain itself without any backing-up of information and findings' of various and comprehensive researches of natural and social sciences. Accordingly, every senior home economist is invited to exert their maximum effort to conduct systematic study with an aim to utilize these findings and information at best in population education in the field of home economics. Therefore, we consider that the development of training material is imminent in order to provide effective and efficient population education through the for training of home economies. It should be noted that these training materials must be carefully designed, tailored and developed to meet the different classes of trainees under the considerations as to whether it is easily adaptable and infusable into the curricula of every field of home economics, and it is acceptable in the degree of difficulty and quality in its contents. 5) It is true that there are many domestic and international research rapers, reports and findings in the field of population education and family planning. However, there is a tendency that the most of research papers are heavily relying on the authors intension and preferences in its expression and publication. Under these circumstances, it is urged that the home economists should aware of the growing need of the technical training in order to keep these available information and research findings reprocessed and redesigned to insure the practical application into the population education in the field of home economics in Korea.