• 대한전기학회논문지:전기물성ㆍ응용부문C
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• 제51권6호
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• pp.265-270
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• 2002

The determination of DNA hybridization reaction can apply the molecular biology research, clinic diagnostics, bioengineering, environment monitoring, food science and application area. So, the improvement of DNA hybridization detection method is very important for the determination of this hybridization reaction. Several molecular biological techniques require accurate predictions of matched versus mismatched hybridization thermodynamics, such as PCR, sequencing by hybridization, gene diagnostics and antisense oligonucleotide probes. In addition, recent developments of oligonucleotide chip arrays as means for biochemical assays and DNA sequencing requires accurate knowledge of hybridization thermodynamics and population ratios at matched and mismatched target sites. In this study, we report the characteristics of the probe and matched, mismatched target oligonucleotide hybridization reaction using thermodynamic method. Thermodynamic of 5 oligonucleotides with central and terminal mismatch sequences were obtained by measured UV-absorbance as a function of temperature. The data show that the nearest-neighbor base-pair model is adequate for predicting thermodynamics of oligonucleotides with average deviations for $\Delta$H$^{0}$ , $\Delta$S$^{0}$ , $\Delta$G$_{37}$ $^{0}$ and T$_{m}$, respectively.>$^{0}$ and T$_{m}$, respectively.

• 문화기술의 융합
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• 제5권3호
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• pp.339-344
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• 2019

서열 정렬에 있어서 전체를 비교하여 두 서열 사이의 최대의 유사성 또는 상동성을 찾는 전역 정렬은 넓은 범위를 선호하게 되는 편향성을 갖게 된다. 비일치 부분을 과감히 제거하고 높은 일치도를 갖는 부분 영역을 정렬하게 되면 정렬점수를 높이는 효과를 갖게 된다. 여러 개의 부분 지역 정렬을 탐색하게 하는 다중 지역정렬 방법을 적용하여 다수의 지역정렬을 수행하는 알고리즘을 구현하고 결과를 분석해 본다. 지역 정렬에 일반적으로 사용되는 Smith-Waterman 알고리즘의 제한점 중 하나인 서열이 길어지는 것을 방지하고, sub-optimal sequence를 찾기 위한 방법을 응용하여 다중지역 정렬을 수행한다.

• Bulletin of the Korean Chemical Society
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• 제32권9호
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• pp.3223-3228
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• 2011

To quantify the presence of mercuric ions in aqueous solution, double-stranded DNA (dsDNA) of poly(dT) was employed using a light switch compound, $Ru(phen)_2(dppz)^{2+}$ (1) which is reported to intercalate into dsDNA of a right-handed B-form. Addition of mercuric ions induced the dehybridization of poly(dT)${\cdot}$poly(dA) duplexes to form a hairpin structure of poly(dT) at room temperature and the metal-to-ligand charge transfer emission derived from the intercalation of 1 was reduced due to the dehybridization of dsDNA. As the concentration of $Hg^{2+}$ was increased, the emission of 1 progressively decreased. This label-free emission method had a detection limit of 0.2 nM. Other metal ions, such as $K^+$, $Ag^+$, $Ca^{2+}$, $Mg^{2+}$, $Zn^{2+}$, $Mn^{2+}$, $Co^{2+}$, $Ni^{2+}$, $Cu^{2+}$, $Cd^{2+}$, $Cr^{3+}$, $Fe^{3+}$, had no significant effect on reducing emission. This emission method can differentiate matched and mismatched poly(dT) sequences based on the emission intensity of dsDNA.