• 제목/요약/키워드: matK

검색결과 1,055건 처리시간 0.026초

The Uneven Distribution of Mating Type Genes in Natural and Cultivated Truffle Orchards Contributes to the Fructification of Tuber indicum

  • Li, Qiang;Fu, Yu;Sun, Qun;Sourzat, Pierre;Yang, Mei;Liu, Chengyi;Tan, Hao;Ye, Lei;Zou, Jie;Wu, Chenguang;Zhang, Bo;Li, Xiaolin
    • Mycobiology
    • /
    • 제46권1호
    • /
    • pp.64-71
    • /
    • 2018
  • The aim of this study was to investigate the pattern of distribution of mating type (MAT) genes of Tuber indicum in ectomycorhizosphere soils from natural T. indicum-producing areas and cultivated truffle orchards and ascocarp samples from different regions. Quantitative real-time PCR and multiplex PCR were used to weight the copy numbers of MAT1-1-1 and MAT1-2-1 in natural truffle soils and cultivated orchard soils. The effect of limestone on the pattern of truffle MAT genes and the correlation between soil properties and the proportion of MAT genes were also assessed. These results indicated that an uneven and nonrandom distribution of MAT genes was common in truffle-producing areas, cultivated truffle orchards, and ascocarps gleba. The competition between the two mating type genes and the expansion of unbalanced distribution was found to be closely related to truffle fructification. Limestone treatments failed to alter the proportion of the two mating type genes in the soil. The content of available phosphorus in soil was significantly correlated with the value of MAT1-1-1/MAT1-2-1 in cultivated and natural ectomycorhizosphere soils. The application of real-time quantitative PCR can provide reference for monitoring the dynamic changes of mating type genes in soil. This study investigates the distributional pattern of T. indicum MAT genes in the ectomycorhizosphere soil and ascocarp gleba from different regions, which may provide a foundation for the cultivation of T. indicum.

Gravel Mat로 보강된 말뚝지지 전면기초의 실내모형실험 (Laboratory Test of Piled-Raft Foundation Improved by Gravel Mat)

  • 서영교;이정훈
    • 한국해양공학회지
    • /
    • 제25권2호
    • /
    • pp.47-54
    • /
    • 2011
  • A piled raft foundation is one of the systems used to reduce the settlement of structures. However, the general design method for a piled raft foundation system assumes that the piles only support external loads, which exclude the bearing capacity of the raft itself. In this study, an experimental model test was performed to evaluate the raft capacity for the external load on the sand. Additionally, a part of the sandy ground under the raft was replaced with a gravel mat to reinforce the piled raft foundation system and increase the bearing capacity. Then, parametric studies of the reinforced ground were performed to determine the displacement and load-sharing ratio of the piled raft foundation system.

A gender identification using shoeprint images

  • Asamizu, Satoshi;Haseyama, Miki
    • 한국방송∙미디어공학회:학술대회논문집
    • /
    • 한국방송공학회 2009년도 IWAIT
    • /
    • pp.699-702
    • /
    • 2009
  • This paper proposes a gender identification using shoeprint images. It is difficult for the proposed method to identify an individual if shoeprint images for identification leaked out. Because the proposed method identifies gender without the faces, the type of dress and the hair types images. Therefore we can use safely the proposed method in public place. In addition, a sensor mat which we developed is reasonable to use mechanical switches arranged in a matrix pattern without pressure switches. We had shoeprint images with the sensor mat. We measure feature parameters from shoeprint images. The feature parameters are length, width and area of shoeprint. Utilizing the feature parameters, we identified gender. In order to verify the gender identification rate of the proposed method, we set up the sensor mat at an entrance of buildings and took shoeprint images of 100 men and 100 women. As a result, we achieved about 86 percent of the gender identification rate.

  • PDF

쇄석배수층에 적용된 토목섬유의 효율성에 대한 실험적 검증 (An Experimental Verification on the Efficiency of Geosynthetics on Crushed Stone Layer)

  • 박민철;임은상;김재홍;한희수
    • 한국지반공학회논문집
    • /
    • 제29권11호
    • /
    • pp.17-27
    • /
    • 2013
  • 본 연구의 목적은 쇄석배수층에 설치된 토목섬유의 효율성을 실험을 통해 검증하고자 하였다. 지지력 보강 역할을 수행하는 PET매트는 재하실험을 수행하여 효율성을 검증하였다. 또한, 폐색 방지 역할을 수행하는 PP매트는 하중재하와 강우 침투, 성토재의 특성에 따른 폐색 조건을 찾고 폐색에 의한 통수능 변화량를 파악하였다. PET매트의 재하실험 결과, PET매트가 설치되지 않으면 쇄석이 점토지반에 관입되며 파괴되었으며, PET매트 설치시 연약지반의 보강효과가 큰 것을 알 수 있었다. 또한, PP매트가 없는 경우에는 성토재의 점착력이 작으면 쇄석배수층이 쉽게 폐색되며, 이로 인해 쇄석배수층의 통수능이 약 98%정도 감소되었다.

초고층 건물의 전면기초(MAT기초) 해석 및 설계 (ANALYSIS AND DESIGN OF MAT FOUNDATION FOR HIGH-RISE BUILDINGS)

  • 홍원기;황대진;권장혁
    • 한국지반공학회:학술대회논문집
    • /
    • 한국지반공학회 1994년도 가을 학술발표회 논문집
    • /
    • pp.94-110
    • /
    • 1994
  • Types of foundation of high rise buildings are primarily determined by loads transmitted from super structure, soil bearing capacity and available construction technology. The usd of deep foundation cannot be justified due to the fact that rock of enough bearing capacity is not found down until 90 ~ 100m. When a concentration of high soil pressure must be distributed over the entire building area, when small soft soil areas must be bridged, and when compressible strata are located at a shallow depth, mat foundation may be useful in order to have settlement and differential settlement of variable soils be minimized. The concept of mat foundation will also demonstrate some difficulities of applications if the load bearing demand directly carried down to the load -bearing strata exceeds the load -bearing capacity. This paper introduces both the analysis and design of mat type foundation for high rise buildings as well as the methodology of modelling of the soil foundation, especially, engineered to redistribute the stress exceeding the soil bearing capadity. This process will result in the wid spread of stresses over the entire building foundation.

  • PDF

교배형 분자마커를 이용한 신품종 밀리타리스 동충하초 '도원홍초 2호'의 품종 특성 (Varietal characteristics of new Cordyceps militaris 'Dowonhongcho 2ho' improved by mating type molecular markers)

  • 이병주;이미애;김용균;이순계;최영상;이병의
    • 한국버섯학회지
    • /
    • 제15권3호
    • /
    • pp.111-117
    • /
    • 2017
  • 본 연구는 밀리타리스 동충하초의 교배형 유전자인 MAT1-1과 MAT1-2 두 종류에 특이적인 프라이머를 사용하여 multiplex PCR을 실시하였고, 그 결과 형성된 MAT1-1과 MAT1-2에 대한 233-bp와 191-bp에서 DNA 밴드를 통해 교배형 및 교배여부를 확인하였고 자실체 특성검정을 통해 품종특성이 우수한 새로운 동충하초 '도원홍초 2호'를 개발하였다. 신품종 '도원홍초 2호'의 자실체는 곤봉형이고 밝은 주황색을 띠었으며, 코디세핀 함량은 0.33%였고 자좌의 굵기와 길이는 각각 3.5 mm와 7.1 cm였다. '도원홍초'와 비교할 때, 새로운 '도원홍초 2호'의 수량은 7%가 증수되었고 경도가 높은 특징을 보였다. 균사생장의 적온은 $20{\sim}25^{\circ}C$였고 버섯 발생의 적온은 $18{\sim}22^{\circ}C$였으며, 접종에서부터 자실체 발생까지의 기간은 49.7일이 소요되었다. 신품종 '도원홍초2호'는 교배형 분자마커를 육종과정에 이용하여 효율성을 높였으며, 우수한 재배적 특성으로 동충하초 인공재배 및 산업적 생산에 기여할 것으로 판단된다.

메트와 필드운동이 노인의 균형과 보행에 미치는 영향 (The effects of mat and field exercise on the balance and gait in older adults)

  • 이수연;마상렬;조교영
    • Journal of the Korean Data and Information Science Society
    • /
    • 제20권4호
    • /
    • pp.661-672
    • /
    • 2009
  • 65세 이상 노인 30명을 대상으로 매트 운동과 필드 운동을 6주간 실시한 후 운동전, 3주 후, 그리고 6주 후에 균형과 보행에 미치는 영향에 대해 알아보기 위해 실시하였다. 측정은 외다리 기립 검사, 버그 균형 검사, 그리고 3차원 동작분석기를 이용하여 보행 분석을 실시하였다. 반복측정자료 분산분석 결과, 시간과 그룹 간 상호작용 효과는 활보길이에서 유의한 차이가 있었고, 외다리 기립, 버그 균형, 분속수, 속도는 유의한 차이가 없었다. 중재기간 별 효과크기를 검정해 본 결과 중재 전과 중재 6주 후, 중재 3주 후와 중재 6주 후에서 유의한 차이가 있었다.

  • PDF