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http://dx.doi.org/10.14480/JM.2017.15.3.111

Varietal characteristics of new Cordyceps militaris 'Dowonhongcho 2ho' improved by mating type molecular markers  

Lee, Byung-joo (Crop Research Division, Chungcheongnam-do Agricultural Research & Extension Services)
Lee, Mi-Ae (Crop Research Division, Chungcheongnam-do Agricultural Research & Extension Services)
Kim, Yong-Gyun (Crop Research Division, Chungcheongnam-do Agricultural Research & Extension Services)
Lee, Sun-Gye (Crop Research Division, Chungcheongnam-do Agricultural Research & Extension Services)
Choi, Young-sang (Yedang Mushroom Institute)
Lee, Byung-eui (Department of Chemistry, College of Natural Science, Soonchunghyang University)
Publication Information
Journal of Mushroom / v.15, no.3, 2017 , pp. 111-117 More about this Journal
Abstract
The mushroom species Cordyceps militaris has been studied and cultivated as a medicinal mushroom due to its multiple valuable biological and pharmaceutical activities. For breeding new strains of C. militaris, multiplex PCR assays were performed using primers specific for its mating type genes, MAT1-1 and MAT1-2. Mating types and mating status were confirmed, as evidenced by DNA bands at 233-bp and 191-bp for MAT1-1 and MAT1-2 respectively. The novel 'Dowonhongcho 2ho' was developed through mating; they were found to possess high-quality fruiting bodies when grown in artificial media. The stromata of the new strain were club-shaped, with a bright orange-red color, and measured 7.1 cm in length. They had an average cordycepin content of 0.33%. Compared to 'Dowonhongcho,' the new strain had a 7% higher yield, as well as firm fruiting bodies. The optimum temperature for mycelial growth was $20{\sim}25^{\circ}C$, and the optimum temperature for stroma development was $18{\sim}22^{\circ}C$. The fruiting bodies developed after 49.1 days from inoculation. The use of mating type molecular markers improved the breeding efficiency of the new strain 'Dowonhongcho 2ho.' Thus, they may be valuable for artificial cultivation and industrial-scale production of C. militaris with excellent characteristics.
Keywords
Cordyceps militaris; Fruiting body; Mating type; Molecular marker; Stroma development;
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