• 한국어병학회지
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• 제34권1호
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• pp.9-15
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• 2021

Rock bream (Oplegnathus fasciatus) is a highly valued aquaculture species in Korea. However, the aquaculture industry suffers huge economic losses due to rock bream iridovirus (RBIV) infection in summer. The objective of this study was to determine genetic diversity and relationships of DNAs isolated from two groups of rock bream after RBIV infection using five microsatellite (MS) markers. The first group of fish died early and the second group of fish died later after RBIV infection. In this experiment, 90 fish (5.1±1.0 cm and 4.1±1.3 g) were injected with 50 μl of RBIV (10⁴ TCID⁵⁰/ml) and maintained at 26℃ for 15 days. Genomic DNAs were extracted from fins of 20 fish that died earlier or later after RBIV infection. These DNAs were subjected to genotyping using five MS markers (CA-03, CA3-05, CA3-06, CA-10, and CA3-36). Of these markers, CA3-05 (early death group), CA3-06 (late death group), and CA3-36 (both early and late death groups) showed different alleles distribution rates. In-depth studies are needed to provide valuable information for selecting RBIV-resistant fish. In conclusion, microsatellite marker distribution pattern differences between early- and late- death groups of rock bream after RBIV infection showing different RBIV susceptibilities were determined using MS markers and genotyping. Results of this study suggest that MS markers could be used to facilitate the selection of RBIV resistant rock bream.

• 대한토목학회논문집
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• 제26권4B호
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• pp.413-420
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• 2006

연안역에 설치되는 구조물로서 경관이나 해수 교환면에서 높은 기능을 가지고 있는 수중방파제의 건설이 증가하고 있다. 수중방파제의 경우, 천단이 정수면 아래에 위치하기 때문에 소형선박의 항해에 대한 표식으로서 일반적으로 부이가 많이 이용되고 있으나, 경관면 뿐만 아니라 쇄파파력에 의한 계류시스템의 파단과 같은 문제점이 지적되고 있다. 이로 인해, 최근에는 계류부이방식 대신에 자연에 가까운 경관을 창조할 수 있는 표식암을 이용하는 경우가 늘고 있으며, 이러한 표식암에 작용하는 파력의 정확한 예측은 설계 시 매우 중요하다. 본 연구에서는 불규칙 파동장을 대상으로 표식암에 작용하는 파력특성을 수리모형실험에 근거하여 검토하였다. 무차원 파력은 수중방파제상의 표식암 설치위치가 연안측으로 이동함에 따라 감소하는 경향을 보였다. 또한, Morison식을 이용하여 얻어진 항력계수와 관성력계수의 변동특성이 K.C.수의 변화와 연관되어 논의되었다.

• 한국어병학회지
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• 제23권3호
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• pp.273-280
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• 2010

To determine whether rock bream Oplegnathus fasciatus can be protected from rock bream iridovirus (RBIV) infection by intramuscular injection of long double-stranded RNAs (dsRNAs), we compared protective effect of virus-specific dsRNAs corresponding to major capsid protein (MCP), ORF 084, ORF 086 genes, and virus non-specific green fluorescent protein (GFP) gene. Furthermore, to determine whether the non-specific type I interferon (IFN) response was associated with protective effect, we estimated the activation of type I IFN response in fish using expression level of IFN inducible Mx gene as a marker. As a result, mortality of fish injected with dsRNAs and challenged with RBIV was delayed for a few days when comparing with PBS injected control group. However, virus-specific dsRNA injected groups exhibited no significant differences in survival period when compared to the GFP dsRNA injected group. Semi-quantitative analysis indicated that the degree of antiviral response via type I IFN response is supposedly equal among dsRNA injected fish. These results suggest that type I IFN response rather than sequence-specific RNA interference might involve in the lengthened survival period of fish injected with virus-specific dsRNAs.

• The Korean Journal of Physiology and Pharmacology
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• 제26권3호
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• pp.175-182
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• 2022

Translocation of azurophil granules is pivotal for bactericidal activity of neutrophils, the first-line defense cells against pathogens. Previously, we reported that lysophosphatidylcholine (LPC), an endogenous lipid, enhances bactericidal activity of human neutrophils via increasing translocation of azurophil granules. However, the precise mechanism of LPC-induced azurophil granule translocation was not fully understood. Treatment of neutrophil with LPC significantly increased CD63 (an azurophil granule marker) surface expression. Interestingly, cytochalasin B, an inhibitor of action polymerization, blocked LPC-induced CD63 surface expression. LPC increased F-actin polymerization. LPC-induced CD63 surface expression was inhibited by both a Rho specific inhibitor, Tat-C3 exoenzyme, and a Rho kinase (ROCK) inhibitor, Y27632 which also inhibited LPC-induced F-actin polymerization. LPC induced Rho-GTP activation. NSC23766, a Rac inhibitor, however, did not affect LPC-induced CD63 surface expression. Theses results suggest a novel regulatory mechanism for azurophil granule translocation where LPC induces translocation of azurophil granules via Rho/ROCK/F-actin polymerization pathway.

• Fisheries and Aquatic Sciences
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• 제11권2호
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• pp.82-87
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• 2008

Marine fisheries are important natural resources and must be maintained, especially fish species that are important sources of food. Despite the increase in stocking programs to maintain fisheries with artificially raised fish, the genetic impact stocking has on the wild fry population has not been addressed. Genetic variation in rock bream, Oplegnathus fasciatus, within and between wild-caught parents and the $F_1$ generation produced by them in 1 day was assayed using nine highly variable micro satellite markers. The nine micro satellite loci used in this study displayed diverse polymorphisms, and in total, 98 different alleles were observed over all loci. Differences in genetic variability of the $F_1$ offspring compared to their wild-caught parents (brood stock) were observed in terms of allele frequency, gene diversity, and heterozygosity. Although the $F_1$ generation of rock bream was missing 16% of the micro satellite alleles, no significant reduction was found in mean heterozygosity of the $F_1$ population compared to the brood stock. Eight of nine loci showed significant Hardy-Weinberg equilibrium (HWE) deviations in the $F_1$ population, while the brood stock deviated from HWE at three micro satellite loci (KOF85, KOF360 and KOF374). These deviations showed mostly a deficit of heterozygotes. Our results provide evidence for genetic differences in the $F_1$ hatchery offspring compared to their wild-caught parents and reinforce the need for a series of consecutive egg collections to avoid the loss of genetic variability. This also further underscores the importance of monitoring genetic variability of hatchery populations for the conservation of natural rock bream resources.

• 한국가금학회:학술대회논문집
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• 한국가금학회 2005년도 제22차 정기총회 및 학술발표회
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• pp.68-69
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• 2005

Dominant white와 Extended black 은 닭의 깃털 색깔에 관여하는 유전자위로서 각각 PMEL l7과 MC1R 유전자를 암호화하며 이들의 염기서열 다형은 닭의 깃털 색깔 양상과 매우 밀접하게 연관되어있다. 본 실험은 이러한 점에 착안하여 PMEL17과 MC1R 유전자의 염기서열에서 Barred Plymouth rock, Korean Ogol Chicken and White Leghorn의 품종 특이적인 염기서열 다형을 확인하였다. PMEL17 유전자에서 8개, MC1R 유전자에서 4개씩 모두 12개의 다형이 확인되었다. White Leghorn은 모든 염기서열 다형 위치에서 다른 두 종의 닭들과 다른 염기를 가지고 있었다. 그러나 Barred Plymouth Rock과 Korean Ogol Chicken은 모든 염기서열 다형 위치에서 같은 염기를 가지고 있었다.

• 농업과학연구
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• 제33권1호
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• pp.1-10
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• 2006

본 연구는 개발된 품종 특이적인 DNA 표지인자를 이용하여 한우와 비한우의 고기를 판별하고자 하였다. 한우와 유럽종 고기소들의 유전적 차이를 Random amplified polymorphic DNA(RAPD) 분석으로 조사한 바, 홀스타인, 헤어포드, 에버딘엥거스, 리모진 및 시멘탈과 같은 한우가 아닌 유럽 종들의 RAPD 패턴들은 동일하였으나, 한우는 이들과 다른 패턴을 보였다. 한우는 유럽종들에서 모두 검출된 특정의 밴드가 발견되지 않았다. 1.4Kbp인 밴드는 한우와 수입소 고기를 판별해 낼 수 있는 유용한 DNA 표지인자로 인정되었다. 실제로, 한우 673 마리, 홀스타인 141 마리의 혈액시료 및 수입육 115의 고기시료를 가지고 시행한 실험에서 그 DNA 표지인자는 비한우에서는 256두 중 245두에서 검출되었으나, 한우에서는 673두 중 644두의 시료에서 검출되지 않아서, 비한우의 검출율은 95%, 한우의 비검출율은 96%를 보였다. 이러한 결과는 그 DNA 표지인자를 이용하여 한우와 비한우 고기를 판별할 수 있음을 시사하였다. 그러나 두 품종 사이의 교잡종은 한우나 비한우의 RAPD 패턴 중에서 한 가지를 무원칙하게 선택하여 나타내고 있으므로, 이러한 DNA 표지인자만으로서는 판별하기가 어려웠다.

• 한국가금학회지
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• 제23권3호
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• pp.135-144
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• 1996

This study was carried out to clarify the genetic constitution of biochemical polymorphic loci controlling blood protein and enzymes as genetic rnarkers in Korean native chicken(KNG) population Blood samples were collected from 230 KNG representing three colored-lines(reddish-, yellowish- and blackish- brown) raised in Daejeon branch of National Livestock Research Institute. Eight blood marker loci, transferrin(Tf), post-albumin(Pas), albumin(Alb), amylase-1(Arny-1), es-terase-1(Es-1), alkaline phosphatase(Akp), catalase(Cat) and hemoglobin(Hh) were analyzed by using starch, agarose and polyacrylamide gel electrophoresis. Based on the gene frequencies of polymorphic marker loci, the genetic characteristics of KNF population was analyzed, and the genetic ariability within population was quantified. The genetic relationships between KNC and other native fowls or improved breeds were also estimated. The gene frequencies of Tf, Pas and AIb loci were similar to those of improved breeds among the seven biochemical polymorphic loci, while gene frequencies of Cat and Es-i loci were remarkably different between KNC and improved breeds. Gene frequencies of amy-i and Akp loci were similar to those of New Hampshire and Rhode Island Red and White Leghorn, respectively. However in comparison with other improved breeds, great differences were observed in gene frequencies of these loci The average heterozygosity, effective number of alleles and homogeneity index for the seven loci combined were estimated to be .334, 1.639 and .373, respectively. Based on the dendrogram and genetic distances, the KNC was genetically closer to New Hampshire, Plymouth Rock and Rhode Island Red breeds than to the White Leghorn breed.

• Animal Systematics, Evolution and Diversity
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• 제27권1호
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• pp.1-17
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• 2011

Thais Roding, 1798, commonly known as rock-shell, is among the most frequently found gastropod genera worldwide on intertidal rocky shores including those of Japan, China, Taiwan and Korea. This group contains important species in many marine environmental studies but species-level taxonomy of the group is quite complicated due to the morphological variations in shell characters. This study examined the genetic variations and relationships among three Korean Thais species based on the partial nucleotide sequences of mitochondrial cox1 gene fragments. Phylogenetic trees from different analytic methods (maximum parsimony, neighbor-joining, and maximum likelihood) showed that T. bronni and T. luteostoma are closely related, indicating the most recent common ancestry. The low sequence divergence found between T. luteostoma and T. bronni, ranging from 1.53% to 3.19%, also corroborates this idea. Further molecular survey using different molecular marker is required to fully understand a detailed picture of the origin for their low level of interspecific sequence divergence. Sequence comparisons among conspecific individuals revealed extensive sequence variations within the three species with maximum values of 2.43% in T. clavigera and 1.37% in both T. bronni and T. luteostoma. In addition, there is an unexpectedly high level of mitochondrial genotypic diversity within each of the three Korean Thais species. The high genetic diversity revealed in Korean Thais species is likely to reflect genetic diversity introduced from potential source populations with diverse geographic origins, such as Taiwan, Hong Kong, and a variety of different coastal regions in South China and Japan. Additional sequence analysis with comprehensive taxon sampling from unstudied potential source populations will be also needed to address the origin and key factors for the high level of genetic diversity discovered within the three Korean Thais species studied.

• 한국수산과학회지
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• 제43권4호
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• pp.340-344
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• 2010

Identification of individuals is a standard tool used to study animal behavior and ecology. Numerous studies based on photographic identifications of various marine mammal populations have been successfully conducted. The objective of this study was to validate a photo-identification method for spotted seals. We tested the method using 4,939 photographs gathered in 30 field surveys from 2006 to 2008 on Baekryongdo, Korea. In 2006, 20 individuals were identified and cataloged using unique spot patterns on their left chick pelages as a natural marker. In 2007 and 2008, three and nine individuals, respectively, were recaptured, with six individuals continually recaptured during the entire survey period. We confirmed that the spot patterns of spotted seal pelages were constant in shape and location throughout time. These characteristics offered an opportunity to consistently identify individuals within a population over the long survey period. The use of photo-identification has great potential for mark-recapture studies.