• Biotechnology and Bioprocess Engineering:BBE
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• 제9권3호
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• pp.217-222
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• 2004

As Agrobacterium tumefaciens, which has long been used to transform plants, is known to transfer T-DNA to budding yeast, Saccharomyces cerevisiae, a variety of fungi were subjected to the A. tumefaciens-mediated transformation to improve their transformation frequency and feasibility. The A. tumefaciens-mediated transformation of chestnut blight fungus, Cryphonectria parasitica, is performed in this study as the first example of transformation of a hardwood fungal pathogen. The transfer of the binary vector pBIN9-Hg, containing the bacterial hygromycin B phosphotransferase gene under the control of the Aspergillus nidulans trpC promoter and terminator, as a selectable marker, led to the selection of more than 1,000 stable, hygromycin B-resistant transformants per 1${\times}$10$\^$6/ conidia of C. parasitica. The putative transformants appeared to be mitotically stable. The transformation efficiency appears to depend on the bacterial strain, age of the bacteria cell culture and ratio of fungal spores to bacterial cells. PCR and Southern blot analysis indicated that the marker gene was inserted at different chromosomal sites. Moreover, three transformants out of ten showed more than two hybridizing bands, suggesting more than two copies of the inserted marker gene are not uncommon.

• 한국HCI학회논문지
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• 제2권2호
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• pp.11-18
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• 2007

본 논문은 영상의 자연스러운 증강을 위하여 패치 기반 텍스쳐 합성에 의한 마커 은닉 방법을 제안한다. 마커는 증강현실을 구성하는 물체의 인식과 추적을 위해 효과적인 도구로 활용되지만, 시각적으로 강조되어야 하는 특성으로 인해 증강현실의 실감성 감소와 사용성 저하를 유발한다는 문제점을 가지고 있다. 제안된 방법은 영상 속의 마커를 대체할 수 있도록 주변 배경과 어울리는 새로운 영상을 합성하여 마커를 은닉한다. 패치 기반의 텍스쳐 합성알고리즘을 사용하여 실시간성을 보장하는 동시에, 배경 텍스쳐의 전역적인 특성을 유지하고, 영상 주변의 조명 변화에 유연하다는 장점이 있다.

• Asian-Australasian Journal of Animal Sciences
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• 제20권3호
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• pp.453-460
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• 2007

An overview of developments important in the future of animal breeding is discussed. Examples from the application of quantitative genetic principles to selection in chickens and mice are given. Lessons to be learned from these species are that selection for production traits in livestock must also consider selection for reproduction and other fitness-related traits and inbreeding should be minimized. Short-term selection benefits of best linear unbiased predictor methodology must be weighed against long-term risks of increased rate of inbreeding. Different options have been developed to minimize inbreeding rates while maximizing selection response. Development of molecular genetic methods to search for quantitative trait loci provides the opportunity for incorporating marker-assisted selection and introgression as new tools for increasing efficiency of genetic improvement. Theoretical and computer simulation studies indicate that these methods hold great promise once genotyping costs are reduced to make the technology economically feasible. Cloning and transgenesis are not likely to contribute significantly to genetic improvement of livestock production in the near future.

• 드라이브 ㆍ 컨트롤
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• 제17권4호
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• pp.8-14
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• 2020

Intelligent devices using ICT technology have been introduced in the field of construction machinery to improve productivity and stability. Among the intelligent devices, Machine Guidance is a device that provides real-time posture, location, and work range to drivers by installing various sensors, controllers, and satellite navigation systems on construction machines. Conversely, the efficiency of equipment that requires location information, such as machine guidance, will be greatly reduced in buildings, and tunnels in the GPS blind spots. Thus, the other high-precision positioning technologies are required in the GPS blind spot zone. In this study, we will develop a relative position measurement system that provides precise location information such as construction machinery and robots in a local area where the GPS reception is difficult. A relative position measurement system tracks a marker in the form of a sphere installed on a vehicle by using the image base tracking technology, and measures the distance and direction information to the marker to calculate a position.

• Journal of Crop Science and Biotechnology
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• 제10권3호
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• pp.123-132
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• 2007

In this review, we discuss the ways in which our understanding of the controls of nitrogen use efficiency applied to crop improvement has been increased through the development of molecular physiology studies using transgenic plants or mutants with modified capacities for nitrogen uptake, assimilation and recycling. More recently, exploiting crop genetic variability through quantitative trait loci and candidate gene detection has opened new perspectives toward the identification of key structural or regulatory elements involved in the control of nitrogen metabolism for improving crop productivity. All together these studies strongly suggest that in the near future nitrogen use efficiency can be improved both by marker-assisted selection and genetic engineering, thus having the most promise for the practical application of increasing the capacity of a wide range of economically important species to take up and utilize nitrogen more efficiently.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2004년도 학술대회 논문집 정보 및 제어부문
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• pp.19-21
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• 2004

In many cases, human's visual recognition depends on contextual information. We need to use effective feature information for performing vigorous place recognition to illumination, noise, etc. In the existing cases that use edge and color, etc., visual recognition doesn't cope effectively with real environment. To solve this problem, using natural marker, we improve the efficiency of place recognition.

• 대한전자공학회:학술대회논문집
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• 대한전자공학회 1998년도 추계종합학술대회 논문집
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• pp.871-874
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• 1998

This paper presents a new morphological spatio-temporal segmentation algorithm. The algorithm incorporates intensity and motion information simultaneously, and uses morphological tools such as morphological filters and watershed algorithm. The procedure toward complete segmetnation consists of three steps: joint marker extraction, boundary decision, and motion-based region fusion. By incorporating spatial and temporal information simultaneously, we can obtain visually meaningful segmentation results. Simulation results demonstrates the efficiency of the proposed method.

• 생명과학회지
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• 제16권3호
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• pp.527-533
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• 2006

벼의 약 및 현미 배양효율과 관련된 DNA marker를 이용하여 인디카형 벼 품종인 'IR 36'의 조직배양 효율을 개선하기 위하여 실험한 결과를 요약하면 다음과 같다. MGRI집단의 약배양에서 식물체분화율이 높은 계통으로 선발된 'MGRI 079'와 'MGRI 036'의 약배양 효율은 각각 19.8%, 19.9%로 가장 높게 나타났다. 'MGRI 079'에 'IR 36'이 여교배되어 양성된 $BC_2F_4$ 100 계통에 대한 marker 검정을 실시하여 선발된 34 계통중 약배양에서 캘러스 형성률이 'IR 36'보다 높은 계통은 30계통이었고, 현미배양에서 'IR 36'보다 식물체 분화율이 높은 계통은 $BC_2F_4-28$ 외 14 계통이었다. $BC_2F_4$ 34계통 중에서 식물체분화능력이 높은 계통으로 선발된 $BC_2F_4-28$은 간장이 'IR 36'보다 큰 편이었으나 출수기와 미립특성은 'IR 36'과 비슷하였다. 'MGRI 036'에 'IR 36'을 반복친으로 여교배하여 양성된 $BC_4F_3$ 50계통의 현미배양을 실시한 결과 'IR 36'보다 식물체 재분화율이 높은 계통은 $BC_4F_3-3$ 외 10 계통이었고, 그중 $BC_4F_3-46$의 식물체 재분화율이 11%로 가장 높게 나타났다.

• 대한토목학회논문집
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• 제35권4호
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• pp.961-968
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• 2015

최근 토목공사의 설계, 시공, 유지관리를 포함한 모든 단계에 BIM을 적용하기 위한 시도가 늘어나고 있다. 또한, 설계기술의 발전과 함께 건설구조물이 대형화, 복잡화 됨으로 인해 유지관리 업무수행을 위한 정보 또한 증가하면서, BIM기반의 유지관리를 위한 정보 활용의 중요성이 대두되고 있다. 본 연구에서는 설계단계에 생성된 BIM정보를 유지관리단계에서 적극 활용하기 위해 증강현실기반 건설 구조물 유지관리 방법론을 제시하고, 이를 통해 비마커기반 증강현실을 이용한 건설 구조물 유지관리 시스템을 구축하고자 한다. 연구에서는 설계단계에서 생성된 3D모델과 시공단계에서 생성된 정보를 유지관리단계에 제공할 수 있게 SURF 알고리즘 기반의 비마커 증강현실 기술을 이용하여 제공함으로써 정보의 활용성 및 운영성을 높여 유지관리의 효율성을 높일 수 있다. 또한, 교량구조물의 3D모델을 증강시켜 구조물의 시각적인 점검이 가능하도록 하였으며, 사례적용을 통해 시스템의 효율성을 확인하였다.

• 한국수정란이식학회지
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• 제32권2호
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• pp.39-45
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• 2017

Mesenchymal stem cells (MSCs) have been considered an alternative source of neuronal lineage cells, which are difficult to isolate from brain and expand in vitro. Previous studies have reported that MSCs expressing Nestin ($Nestin^+$ MSCs), a neuronal stem/progenitor cell marker, exhibit increased transcriptional levels of neural development-related genes, indicating that $Nestin^+$ MSCs may exert potential with neurogenic differentiation. Accordingly, we investigated the effects of the presence of $Nestin^+$ MSCs in bone-marrow-derived primary cells (BMPCs) on enhanced neurogenic differentiation of BMPCs by identifying the presence of $Nestin^+$ MSCs in uncultured and cultured BMPCs. The percentage of $Nestin^+$ MSCs in BMPCs was measured per passage by double staining with Nestin and CD90, an MSC marker. The efficiency of neurogenic differentiation was compared among passages, revealing the highest and lowest yields of $Nestin^+$ MSCs. The presence of $Nestin^+$ MSCs was identified in BMPCs before in vitro culture, and the highest and lowest percentages of $Nestin^+$ MSCs in BMPCs was observed at the third (P3) and fifth passages (P5). Moreover, significantly the higher efficiency of differentiation into neurons, oligodendrocyte precursor cells and astrocytes was detected in BMPCs at P3, compared with P5. In conclusion, these results demonstrate that neurogenic differentiation can be enhanced by increasing the proportion of $Nestin^+$ MSCs in cultured BMPCs.