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Expression of peroxiredoxin I regulated by gonadotropins in the rat ovary

  • Lee, Yu-Il;Kang, Woo-Dae;Kim, Mi-Young;Cho, Moon-Kyoung;Chun, Sang-Young
    • Clinical and Experimental Reproductive Medicine
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    • v.38 no.1
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    • pp.18-23
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    • 2011
  • Objective: Peroxiredoxins (Prxs) play an important role in regulating cellular differentiation and proliferation in several types of mammalian cells. This report examined the expression of Prx isotype I in the rat ovary after hormone treatment. Methods: Immature rats were injected with 10 IU of pregnant mare's serum gonadotropin (PMSG) to induce the growth of multiple preovulatory follicles and 10 IU of human chorionic gonadotropin (hCG) to induce ovulation. Immature rats were also treated with diethylstilbestrol (DES), an estrogen analogue, to induce the growth of multiple immature follicles. Northern blot analysis was performed to detect gene expression. Cell-type specific localization of Prx I mRNA were detected by in situ hybridization analysis. Results: During follicle development, ovarian Prx I gene expression was detected in 3-day-old rats and had increased in 21-day-old rats. The levels of Prx I mRNA slightly declined one to two days following treatment with DES. A gradual increase in Prx I gene expression was observed in ovaries obtained from PMSG-treated immature rats. Furthermore, hCG treatment of PMSG-primed rats resulted in a gradual stimulation of Prx I mRNA levels by 24 hours (2.1-fold increase) following treatment, which remained high until 72 hours following treatment. In situ hybridization analysis revealed the expression of the Prx I gene in the granulosa cells of PMSG-primed ovaries and in the corpora lutea of ovaries stimulated with hCG for 72 hours. Conclusion: These results demonstrate the gonadotropin and granulosa cell-specific stimulation of Prx I gene expression, suggesting its role as a local regulator of follicle development.

Establishment of Effective Mouse Model of Premature Ovarian Failure Considering Treatment Duration of Anticancer Drugs and Natural Recovery Time

  • Lee, Eun hee;Han, Si Eun;Park, Min Jung;Kim, Hyeon Jung;Kim, Hwi Gon;Kim, Chang Woon;Joo, Bo Sun;Lee, Kyu Sup
    • Journal of Menopausal Medicine
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    • v.24 no.3
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    • pp.196-203
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    • 2018
  • Objectives: This study was aimed to establish the most effective premature ovarian failure (POF) mouse model using Cyclophosphamide (CTX), busulfan (Bu), and cisplatin considering treatment duration of anticancer drugs and natural recovery time. Methods: POF was induced by intraperitoneally injecting CTX (120 mg/kg)/Bu (12 mg/kg) for 1 to 4 weeks or cisplatin (2 mg/kg) for 3 to 14 days to C57BL/6 female mice aged 6 to 8 weeks. Controls were injected with equal volume of saline for the same periods. Body weight was measured every week, and ovarian and uterine weights were measured after the last injection of anticancer drug. To assess ovarian function, POF-induced mice were superovulated with pregnant mare serum gonadotropin and human chorionic gonadotropin, and then mated with male. After 18 hours, zygotes were retrieved and cultured for 4 days. Finally, the mice were left untreated for a period of times after the final injection of anticancer drug, and the time for natural recovery of ovarian function was evaluated. Results: After 2 weeks of CTX/Bu injection, ovarian and uterine weights, and ovarian function were decreased sharply. Cisplatin treatment for 10 days resulted in a significant decrease in ovarian and uterine weight, and ovarian function. When POF was induced for at least 2 weeks for CTX/Bu and for at least 10 days for cisplatin, ovarian function did not recover naturally for 2 weeks and 1 week, respectively. Conclusions: These results suggest that CTX/Bu should be treated for at least 2 weeks and cisplatin for at least 10 days to establish the most effective primary ovarian insufficiency mouse model.

Topical application of cervix with hyaluronan improves fertility in goats inseminated with frozen-thawed semen

  • Leethongdee, Sukanya;Thuangsanthia, Anone;Khalid, Muhammad
    • Animal Bioscience
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    • v.34 no.6
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    • pp.985-992
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    • 2021
  • Objective: Artificial insemination plays an important role in genetic improvement in the goat farming system. The aim of this study was to investigate the effect of cervical application of hyaluronan (HA) on the fertility in goats after cervical artificial insemination using frozen-thawed (F-T) semen. Methods: After oestrous synchronisation with progesterone sponges and pregnant mare serum gonadotropin injection, both nulli- and multi-parous goats, were randomly allocated to 2 groups, and were inseminated with 0.25 mL of F-T semen (150×106 spermatozoa) twice at 52 h and 56 h after sponge removal. Prior to the insemination, goats in Group 1 only were given topical cervical HA application at 48 h after sponge removal. Site of insemination was recorded as os-cervix or intra-cervix or intra-uterus. Pregnancy was tested ultrasonographically 42 days after insemination. The data on pregnancy rates and percentage of animals according to the site of semen deposition were compared by Chi-square analysis. Results: The overall pregnancy rate was significantly (p<0.004) higher in goats with prior application to the cervix with HA (63.3%) than without (36.0%). Same pattern was observed in the pregnancy rates of nulli- and multi-parous goats in both the groups. Percentage of nulliparous goats according to the site of insemination in the HA group did not differ between first and the second insemination. However, in multiparous goats the percentage of animals inseminated intra-cervically was significantly increased (p≤0.05) between the first and the second inseminations. Conclusion: The results suggest that significantly higher fertility rate in the "HA goats" compared to the "without HA" group was because of deeper insemination facilitated by topical cervical application of HA. The deeper insemination into the cervical canal increase the rate of fertilisation when the cervical artificial insemination is performed.

Effects of Repeated Ovarian Stimulation on Ovarian Function and Aging in Mice

  • Whang, Jihye;Ahn, Cheyoung;Kim, Soohyun;Seok, Eunji;Yang, Yunjeong;Han, Goeun;Jo, Haeun;Yang, Hyunwon
    • Development and Reproduction
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    • v.25 no.4
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    • pp.213-223
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    • 2021
  • Controlled ovarian hyperstimulation (COH) is routinely used in the in vitro fertilization and embryo transfer (IVF-ET) cycles to increase the number of retrieved mature oocytes. However, the relationship between repeated COH and ovarian function is still controversial. Therefore, we investigated whether repeated ovarian stimulation affects ovarian aging and function, including follicular development, autophagy, and apoptosis in follicles. Ovarian hyperstimulation in mice was induced by intraperitoneal injection with pregnant mare serum gonadotropin (PMSG) and human chorionic gonadotropin (hCG). Mice subjected to ovarian stimulation once were used as a control group and 10 times as an experimental group. Repeated injections with PMSG and hCG significantly reduced the number of primary follicles compared to a single injection. The number of secondary and antral follicles increased slightly, while the number of corpus luteum increased significantly with repeated injections. On the other hand, repeated injections did not affect apoptosis in follicles associated with follicular atresia. The expression of autophagy-related genes Atg5, Atg12, LC3B, and Beclin1, cell proliferation-related genes mTOR, apoptosis-related genes Fas, and FasL was not significantly different between the two groups. In addition, the expression of the aging-related genes Dnmt1, Dnmt3a, and AMH were also not significantly different. In this study, we demonstrated that repeated ovarian stimulation in mice affects follicular development, but not autophagy, apoptosis, aging in ovary. These results suggest that repetition of COH in the IVF-ET cycle may not result in ovarian aging, such as a decrease in ovarian reserve in adult women.

Induction of superovulation in mature mice and rats using serum of spayed female dogs

  • Nooranizadeh, MH;Mogheiseh, A;Kafi, M;Sepehrimanesh, M;Vaseghi, H
    • Laboraroty Animal Research
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    • v.34 no.4
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    • pp.211-215
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    • 2018
  • The following experiments were designed to examine the effect of serum of spayed dogs on superovulation response in mice and rats. In Experiment 1, female mice at diestrus (n=30) were divided into three equal groups and superovulated with either administration of 5 IU pregnant mare serum gonadotropin (PMSG) or recombinant follicle stimulating hormone (rFSH) (reducing dose from 2.5 to 0.5 IU) and 5 IU human chorionic gonadotropin (hCG) administered 48h later. Serum of spayed dogs was administered intraperitoneally at a reduced dose from 0.1 to 0.025 mL in a 48 h period. In Experiment 2, female rats (n=30) at diestrus stage were divided into three equal groups. Superovulation was induced using either 30 IU PMSG, or a dose reduced from 5 to 1 IU rFSH and 25 IU hCG administered 48h later. Serum of spayed dogs was administered in a reduced dose from 0.6 to 0.1 mL in a 48 hour period. Female mice and rats were mated 24 h following hCG administration. On day 14 after mating, animals were euthanized and ovarian sections were fixed for histopathological evaluation and corpus luteum (CL) counting. No significant difference observed in mean (${\pm}SEM$) number of CLs between the PMSG group and the mice that received serum of spayed dog ($10.4{\pm}1.3$ vs $9.2{\pm}1.0$). Mean (${\pm}SEM$) number of CLs tended to be lower in rats that received serum of spayed dog than those of rats which received either PMSG or rFSH ($15.1{\pm}1.9$ vs $23.6{\pm}3.1$ and $23.1{\pm}2.9$, P=0.06, respectively). In conclusion, serum of spayed dogs is able to induce a superovulatory response in mice and rats.

Differential Effects of Gonadotropin-Releasing Hormone(GnRH) Agonist on Ovarian Function in Early and Late Follicular Phase of Pregnant Mare Serum Gonadotropin (PMS G) -Pretreated Immature Rats (PMSG로 전처치한 미성숙 래트의 초기 및 후기 난포기에 있어서 GnRH Agonist가 난소 기능에 미치는 상이 효과)

  • Yun, S.K.;Yu, W.J.;Yun, Y.W.
    • Journal of Embryo Transfer
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    • v.13 no.3
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    • pp.261-275
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    • 1998
  • 본 연구에서는 GnRH가 과배란 처치된 래트의 초기 난포기와 후기 난포기에서 난소기능에 어떠한 영향을 미치는지를 이해하기 위해서, 30IU PMSG와 10IU hCG로 전처치된 미성숙 래트에 있어서 배란반응, 배란 난자의 형태학적 이상 유무 및 핵 성숙도, 난소 중량, 난소의 조직학적인 변화 및 혈중 스테로이드 호르몬 (17$\beta$-estradiol, progesterone 및 testosterone) 농도에 대하여 GnRH agonist의 효과를 검사하였다. GnRH agonist는 PMSG 전처치 후 초기 난포기 (PMSG 투여 후 6시간부터) 또는 후기 난포기(PMSG 투여 후 54시간부터)에 4시간 동안 20분 간격으로 경정맥 카테타를 통해 혈관내로 투여하였다. 각 실험동물은 혈중 스테로이드 호르몬의 변화를 측정하기 위하여 PMSG 투여 후 54시간, 72시간에 혈액을 채취하고 72시간에 희생시켰다. PMSG로 전처치한 미성숙 래트의 초기 난포기에 GnRH agonist의 투여는 GnRH agonist를 투여하지 않은 군(대조군)에 비해 과배란 억제, 형태학적 비정상 배란난자의 증가, 난소 중량의 감소, 난포폐쇄의 증가 및 혈중 스테로이드 호르몬의 농도 감소가 보였다. 한편 후기 난포기에 GnRH agonist의 투여는 대조군에서의 반응과 전반적으로 유사하였다. 이상의 결과, PMSG 및 hCG 처치로 과배란된 래트의 초기 난포기에 GnRH agonist의 투여는 난소기능을 전반적으로 억제하지만, 후기 난포기에 GnRH agonist의 투여는 난소기능에 영향을 미치지 않았다.

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L'identità e l'ibridità oltre il confine della Mitteleuropa danubiana in Danubio di Claudio Magris (클라우디오 마그리스의 『다뉴브강』에 나타난 다뉴브강 중부유럽의 탈경계적인 정체성과 혼종성)

  • Lee, Seung Su
    • Lettere Italiane
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    • no.36
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    • pp.73-97
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    • 2012
  • Danubio è un 'diario di viaggio' dalle sorgenti del fiume fino al Mare Nero, attraverso città importantissime o paesi, ampi paesaggi, popoli, costumi, letterature e lingue diverse. Un itinerario fra romanzo e saggio che racconta la cultura come esperienza esistenziale e ricostruisce a mosaico le civiltà di Mitteleuropa rintracciandone il profilo nei segni della grande storia e nelle effimere tracce della vita quotidiana. L'idea di Mitteleuropa nasce a meta dell'Ottocento per indicare un spazio politico e soprattutto economico egemonizzato dai tedeschi e dagli ungheresi, che piu tardi diventa il simbolo di programmi nazionalisti tedeschi e che poi diventa una dimensione sovranazionale, qualcoca di comune e sottostante a tutte le diverse nazionalità e culture di tante realtà diverse. La Mitteleuropa 'hinternazionale', oggi idealizzata quale armonia di popoli diversi, è stata una realtà dell'impero absburgico, nella sua ultima stagione, una tolleranzte convivenza comprensibiilmente rimpianta dopo la sua fine, anche per il confronto con la barbarie totalitaria che le è succeduta, fra le due guerre mondiali, nello spazio danubiano. L'arte absburgica di governo non vuole imporre una rigida unità ai vari popoli, bensi lasciarli sussistere e convivere nella loro eterogeneità. Secondo Claudio Magris, il Danubio è il simbolo della frontiera, perché il Danubio è un fiume che passa attraverso tante frontiere, è quindi simbolo della necessità e della difficoltà di attraversare frontiere, non soltanto nazionali, politiche, sociali, ma anche psicologiche, culturali, religiose. La nostra identità è sempre fragile e noi dobbiamo accettare questa fragilità, poiché mutiamo nel tempo. L'identità fatta di mescolanze, di sottrazioni e di elisioni non fosse soltanto il destino degli epigoni danubiani, bensì una condizione storica generale, l'esistenza di ogni individuo. Magris dice che siccome nessun popolo, nessuna cultra come nessun individuo sono privi di colpe storiche, rendersi impietosamente conto dei difetti e delle oscurità di tutti, e di se stessi, può essere una proficua premessa di convivenza civile e tollerante. Magris dice che la letteratura è di perse stessa una frontiera, una soglia, una zona sul limitare e insegna a varcare i limiti, ma consiste nel tracciare dei limiti, senza i quali non puo esistere e nemmeno la tensione a superarli per raggiungere qualcosa di piu alto e di piú umano.

Pathological fracture induced by Halicephalobus gingivalis (Nematoda: Rhabditida) in a horse limb

  • Ticiana Meireles Sousa;Hugo Shisei Toma;Antonio de Padua Lima;Antonio Carlos Cunha Lacreta Junior;Maira Meira Nunes;Ana Paula Cassiano da Silva;Daniel Wouters;Adriana Silva Albuquerque;Mary Suzan Varaschin;Djeison Lutier Raymundo;Claudia Dias Monteiro Toma;Fernando Arevalo Batista
    • Parasites, Hosts and Diseases
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    • v.62 no.1
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    • pp.131-138
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    • 2024
  • Halicephalobus gingivalis is a free-living nematode that occasionally causes infections in horses. We report a rare case of limb fracture of horse caused by infection with H. gingivalis. An 8-year-old mare was referred to the Veterinary Hospital of the Federal University of Lavras with claudication grade 5 of the right hind limb, that had been started 3 months ago. The patient had aseptic arthritis in the tarsal joint and edema that extended to the quartile. The radiographic examination showed punctate osteolysis with exacerbation of bone trabeculation along the calcaneus, talus, proximal epiphysis of the third metatarsal and distal epiphysis of the tibia. Treatment for arthritis was initiated, and the animal showed a slight improvement in limb function. However, 21 days after hospitalization, due to a comminuted fracture of the tibia, it was euthanized. At necropsy, yellowish masses were found from the metatarsal to the tibia, and around the tarsal bones and joint. Similar masses were also found in the left kidney. Numerous nematodes compatible with H. gingivalis were identified. This is the first description of a pathological fracture caused by H. gingivalis infection in an equine limb.

Gonadotropins Regulate the mRNA Expression of Gonadotropin-Releasing Hormone and Its Receptors in the Mouse Ovary and Uterus

  • Soeun Moon;Bokyeong Yun;Minju Lee;Eunji Seok;Jinah Ha;Hyunwon Yang
    • Development and Reproduction
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    • v.28 no.1
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    • pp.1-12
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    • 2024
  • Gonadotropin-releasing hormone (GnRH), a critical hormone produced in the hypothalamus, is essential for regulating reproductive processes. It has also been demonstrated the presence of GnRH and its receptors (GnRHR) in ovarian and uterine tissues, but little was known about the regulation mechanism of their expression in these organs and ovarian aging. Therefore, the aim of this study was to investigate the expression of GnRHR in the ovary and uterus of mice, particularly after high-dose gonadotropin treatments and in relation to aging. Quantitative real-time-PCR (qRT-PCR) revealed that pituitary gland had the highest GnRHR expression in both young and aged mice. In addition, liver expression was higher in young mice, whereas thymus expression was higher in aged mice. GnRHR mRNA was present in the ovaries of both young and aged mice but nearly undetectable in the uterus of aged mice. We next examined the expression of GnRHR in the ovary and uterus in response to high-dose administration of pregnant mare serum gonadotropin (PMSG). After PMSG administration, GnRH mRNA levels were significantly decreased in the ovary but increased in the uterus. The expression of GnRH mRNA in these organs showed opposite trends to that of GnRHR expression. These results suggest the involvement of GnRH in age-related reproductive decline and the potential effects of high-dose gonadotropin treatments on reproductive organ function.

The effects of superovulatory doses of pregnant mare serum gonadotropin on uterine microenvironment of the rat (다배란 용량의 임마혈청성 고나도트로핀(PMSG)이 랫트의 자궁내 미세환경에 미치는 영향)

  • Yun, Young-won
    • Korean Journal of Veterinary Research
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    • v.34 no.4
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    • pp.745-757
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    • 1994
  • Superovulatory treatment with exogenous gonadotropins adversely affects the uterus through the disruption of the delicate balance of ovarian steroids (estrogens, progestins, androgens). To examine the uterine effects of this treatment, 189 rats were given 4IU, 20IU or 40IU pregnant mare',s serum gonadotropin(PMSG) at 28 days of age and sacrificed every 24h until day 10(D10) post injection. Long term uterine effects were examined in 12 rats treated with 4IU or 40IU PMSG and killed on D30. Adult rat uteri were examined to provide a reference for comparisons. Morphological and histological changes of control (4IU) uteri mimicked those of the adult on a comparable time-course form D2 to D5. Administration of superovulatory doses(20IU, 40IU) of PMSG produced stromal hypertrophy by D2 and focal papillary hyperplasia of the luminal epithelia by D3. Levels of $17{\beta}$-estradiol following 20IU and 40IU PMSG treatment were significantly(p<0.05, p<0.005) elevated above those of controls after D1. Androgen levels of both groups(20IU, 40IU) significantly p<0.05, p<005 increased from baseline on D1 and were maximum between D2 and D3. In the 20IU PMSG group, the hyperplasia gradually regressed after D3 and was absent by D10. The hyperplasia in the 40IU PMSG group, however, had become extensive by D6. It is suspected that preceding elevated levels of estrogen may be responsible for this progressive change. On D 4, the levels of $17{\beta}$-estradiol reached a maximum, which was significantly(p<0.001) greater than both the controls and 20IU PMSG-treated rats. Between D6 and D10, the hyperplasia in 40IU PMSG-treated rats partially regressed. Examination of uteri from D30 revealed no evidence of the hyperplasia. It is suggested that previous exposure to high levels of estrogen and androgens, secondary to superovulation, is possible cause for the observed hyperplasia.

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