• Title/Summary/Keyword: mapping method

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Plant genome analysis using flow cytometry

  • Lee Jai-Heon;Kim Kee-Young;Chung Dae-Soo;Chung Won Bok;Kwon Oh-Chang
    • Proceedings of the Korean Society of Crop Science Conference
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    • 1999.05a
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    • pp.162-163
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    • 1999
  • The goal of this research was (1) to describe the conditions and parameters required for the cell cycle synchronization and the accumulation of large number of metaphase cells in maize and other cereal root tips, (2) to isolate intact metaphase chromosomes from root tips suitable for characterization by flow cytometry, and (3) to construct chromosome-specific libraries from maize. Plant metaphase chromosomes have been successfully synchronized and isolated from many cereal root-tips. DNA synthesis inhibitor (hydroxyurea) was used to synchronize cell cycle, follwed by treatement with trifluralin to accumulate metaphase chromosomes. Maize flow karyotypes show substantial variation among inbred lines. thish variation should be sueful in isolating individual chromosome types. In addition, flow cytometry is a useful method to measure DNA content of individual chromosomes in a genotyps, and to detect chromosomal variations. Individual chromosome peaks have been sorted from the maize hybrid B73/Mol7. Libraries were generated form the DOP-PCR amplification product from each peak. To date, we have analyzed clones from a library constructed from the maize chromosome 1 peak. Hybridization of labeled genomic DNA to clone inserts indicated that $24\%,\;18\%,\;and\;58\%$ of the clones were highly repetitive, medium repetitive, and low copy, respectively. Fifty percent of putative low cpoy clones showed single bands on inbred screening, blots, and the remaining $50\%$ were low copy repeats. Single copy clones showing polymorphism will be mapped using recombinant inbred mapping populations. Repetitive clones are being characterized by Southern blot analysis, and will be screened by in situ hybridization for their potential utility as chromosome specific markers.

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Transforming XML DTD to SQL Schema based on JDBC (XML DTD의 JDBC 기반 SQL 스키마로의 변환)

  • 이상태;주경수
    • Journal of Internet Computing and Services
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    • v.3 no.1
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    • pp.29-40
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    • 2002
  • The information exchange on the using of XML such as B2B electronic is common. So the efficient method to store XML message in database is needed. Because the ORDBMS is extended to ORDBMS for supporting multimedia application such as Oracle8i, 9i, Informix and SQL2000 server, SQL2, the standard RDB is extended to SQL3 for ORDB, And the XML application based on java such as J2EE is extended. Therefor it is necessary for the efficient connection methods based on JDBC between XML application and database system. In this paper, the methodology a transformation XML DTD to SQL3 schema is proposed. For the transformation, first the methods of transformation XML DTD to object model in UML class diagram are proposed. And then the methods of mapping transferred object models to SQL3 schema are proposed. This approach for transform::1lion XML. DTD to SQL3 schema such as Oracle8i, 9i, Informix and SQL2000 server based on java is proposed in this paper, can be used in database design to build XML applications based on ORDB.

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Semi-fragile Watermarking Scheme for H.264/AVC Video Content Authentication Based on Manifold Feature

  • Ling, Chen;Ur-Rehman, Obaid;Zhang, Wenjun
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.8 no.12
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    • pp.4568-4587
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    • 2014
  • Authentication of videos and images based on the content is becoming an important problem in information security. Unfortunately, previous studies lack the consideration of Kerckhoffs's principle in order to achieve this (i.e., a cryptosystem should be secure even if everything about the system, except the key, is public knowledge). In this paper, a solution to the problem of finding a relationship between a frame's index and its content is proposed based on the creative utilization of a robust manifold feature. The proposed solution is based on a novel semi-fragile watermarking scheme for H.264/AVC video content authentication. At first, the input I-frame is partitioned for feature extraction and watermark embedding. This is followed by the temporal feature extraction using the Isometric Mapping algorithm. The frame index is included in the feature to produce the temporal watermark. In order to improve security, the spatial watermark will be encrypted together with the temporal watermark. Finally, the resultant watermark is embedded into the Discrete Cosine Transform coefficients in the diagonal positions. At the receiver side, after watermark extraction and decryption, temporal tampering is detected through a mismatch between the frame index extracted from the temporal watermark and the observed frame index. Next, the feature is regenerate through temporal feature regeneration, and compared with the extracted feature. It is judged through the comparison whether the extracted temporal watermark is similar to that of the original watermarked video. Additionally, for spatial authentication, the tampered areas are located via the comparison between extracted and regenerated spatial features. Experimental results show that the proposed method is sensitive to intentional malicious attacks and modifications, whereas it is robust to legitimate manipulations, such as certain level of lossy compression, channel noise, Gaussian filtering and brightness adjustment. Through a comparison between the extracted frame index and the current frame index, the temporal tempering is identified. With the proposed scheme, a solution to the Kerckhoffs's principle problem is specified.

Geometric calibration of digital photogrammetric camera in Sejong Test-bed (세종 테스트베드에서 항측용 디지털카메라의 기하학적 검정)

  • Seo, Sang-Il;Won, Jae-Ho;Lee, Jae-One;Park, Byoung-Uk
    • Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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    • v.30 no.2
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    • pp.181-188
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    • 2012
  • The most recent, Digital photogrammetric camera, Airborne LiDAR and GPS/INS same sensors are used to acquire spatial information of various kinds in the field of aerial survey. In addition, Direct Georeferencing technology has been widely utilized with digital photogrammetric camera and GPS/INS. However, the sensor Calibration to be performed according to the combination of various sensors is followed by problems. Most of all, boresight calibration of integrated sensors is a critical element in the mapping process when using direct georeferencing or using the GPS/INS aerotriangulation. The establishment of a national test-bed in Sejong-si for aerial sensor calibration is absolutely necessary to solve this problem. And accurate calibration with used to integration of GPS/INS by aerotriangulation of aerial imagery was necessary for determination of system parameters, evaluation of systematic errors. Also, an investigation of efficient method for Direct georeferencing to determine the exterior orientation parameters and assessment of geometric accuracy of integrated sensors are performed.

Task-to-Tile Binding Technique for NoC-based Manycore Platform with Multiple Memory Tiles (복수 메모리 타일을 가진 NoC 매니코어 플랫폼에서의 태스크-타일 바인딩 기술)

  • Kang, Jintaek;Kim, Taeyoung;Kim, Sungchan;Ha, Soonhoi
    • Journal of KIISE
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    • v.43 no.2
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    • pp.163-176
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    • 2016
  • The contention overhead on the same channel in an NoC architecture can significantly increase a communication delay due to the simultaneous communication requests that occur. To reduce the overall overhead, we propose task-to-tile binding techniques for an NoC-based manycore platform, whereby it is assumed that the task mapping decision has already made. Since the NoC architecture may have multiple memory tiles as its size grows, memory clustering is used to balance the load of memory by making applications access different memory tiles. We assume that the information on the communication overhead of each application is known since it is specified in a dataflow task graph. Using this information, this paper proposes two heurisitics that perform binding of multiple tasks at once based on a proper memory clustering method. Experiments with an NoC simulator prove that the proposed heurisitic shows performance gains that are 25% greater than that of the previous binding heuristic.

A Study on the Mapping of Wind Resource using Vegetation Index Technique at North East Area in Jeju Island (영상자료의 식생지수를 이용한 제주 북동부 지역의 풍력자원지도 작성에 관한 연구)

  • Byun, Ji Seon;Lee, Byung Gul;Moon, Seo Jung
    • Journal of Korean Society for Geospatial Information Science
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    • v.23 no.1
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    • pp.15-22
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    • 2015
  • To create a wind resource map, we need a contour map, a roughness map and wind data. We need a land cover map for the roughness map of these data. A land cover map represents the area showing similar characteristics after color indexing based on the scientific method. The features of land cover is classified by Remote sensing technique. In this study, we verified the application of the NDVI technique is reasonable after we created the wind resource map using roughness maps by unsupervised classification and NDVI technique. As a result, the wind resource map using the NDVI technique showed a 60% accordance rate and difference in class less than one. From the results, The NDVI technique is found alternative to create roughness maps by the unsupervised classification.

A Wavelet Based Robust Logo Watermarking Algorithm for Digital Content Protection (디지털 콘텐트 보호를 위한 강인한 웨이블릿 기반 로고 워터마킹 알고리즘)

  • Kim, Tae-Jung;Hwang, Jae-Ho;Hong, Choong-Seon
    • Journal of Internet Computing and Services
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    • v.9 no.1
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    • pp.33-41
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    • 2008
  • Due to the advantage of wavelet transform such as the compatibility with JPEG2000, multi-resolution decomposition, and application of HVS(Human Visual System), watermarking algorithm based on wavelet transform (DWT) is recently mast interesting research subject. However, mast of researches are focused on theoretical aspects for the robustness rather than practical usage, and. may be not suitable too much complicated to use in practice. In this paper, robust logo watermarking algorithm based on DWT is proposed for large and huge data processing. The proposed method embeds the logo watermark by mapping of $8{\times}8$ blocks in order of the number of '1' of the original image and the randomized watermark image with LFSR. The original image is transformed by 2 level wavelet. The experimental results shows that the watermark is embedded successfully, and the proposed algorithm has the valuable robustness from the image processing like JPEG compression, low pass filter, high pass filter and changes in brightness and contrast.

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3-Dimensional Building Reconstruction with Airborne LiDAR Data

  • Lee, Dong-Cheon;Yom, Jae-Hong;Kwon, Jay-Hyoun;We, Gwang-Jae
    • Korean Journal of Geomatics
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    • v.2 no.2
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    • pp.123-130
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    • 2002
  • LiDAR (Light Detection And Ranging) system has a profound impact on geoinformatics. The laser mapping system is now recognized as being a viable system to produce the digital surface model rapidly and efficiently. Indeed the number of its applications and users has grown at a surprising rate in recent years. Interest is now focused on the reconstruction of buildings in urban areas from LiDAR data. Although with present technology objects can be extracted and reconstructed automatically using LiDAR data, the quality issue of the results is still major concern in terms of geometric accuracy. It would be enormously beneficial to the geoinformatics industry if geometrically accurate modeling of topographic surface including man-made objects could be produced automatically. The objectives of this study are to reconstruct buildings using airborne LiDAR data and to evaluate accuracy of the result. In these regards, firstly systematic errors involved with ALS (Airborne Laser Scanning) system are introduced. Secondly, the overall LiDAR data quality was estimated based on the ground check points, then classifying the laser points was performed. In this study, buildings were reconstructed from the classified as building laser point clouds. The most likely planar surfaces were estimated by the least-square method using the laser points classified as being planes. Intersecting lines of the planes were then computed and these were defined as the building boundaries. Finally, quality of the reconstructed building was evaluated.

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Extraction and Modeling of Curved Building Boundaries from Airborne Lidar Data (항공라이다 데이터의 건물 곡선경계 추출 및 모델링)

  • Lee, Jeong Ho;Kim, Yong Il
    • Journal of Korean Society for Geospatial Information Science
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    • v.20 no.4
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    • pp.117-125
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    • 2012
  • Although many studies have been conducted to extract buildings from airborne lidar data, most of them assume that all the boundaries of a building are straight line segments. This makes it difficult to model building boundaries containing curved segments correctly. This paper aims to model buildings containing curved segments as combination of straight lines and arcs. First, two sets of boundary points are extracted by adaptive convex hull algorithm and local convex hull algorithm with a larger radius. Then, arc segments are determined by average spacing of boundary points and intersection ratio of perpendicular lines. Finally, building boundary is modeled through regularization of least squares line or circle fitting. The experimental results showed that the proposed method can model the curved building boundaries as arc segments correctly by completeness of 69% and correctness of 100%. The approach will be utilized effectively to create automatically digital map that meets the conditions of the Korean digital mapping.

Optical imaging methods for qualification of superconducting wires

  • Kim, Gracia;Jin, Hye-Jin;Jo, William
    • Progress in Superconductivity and Cryogenics
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    • v.16 no.4
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    • pp.21-25
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    • 2014
  • In order to develop 2nd generation (2G) high-temperature superconducting (HTS) wires as commercial products, it is necessary to perform a high speed investigation of their superconducting performance. Room-temperature and non-contact optical scanning tools are necessary to verify the microstructure of the superconducting materials, the current flow below the critical temperature, and the critical current density. In this paper, we report our results of an inspection of the electrical transport properties of coated conductors. The samples that we used in our study were highly qualified rare-earth based coated conductors produced via co-evaporation, and $SmBa_2Cu_3O_{7-y}$ (SmBCO) was the superconducting materials used in our studies. A film grown on IBAD-MgO templates shows larger than 400 A/cm at 77 K and a self-field. The local transport properties of the films were investigated by room-temperature imaging by thermal heating. The room-temperature images show structural inhomogeneities on the surface of the films. Bolometric response imaging via low-temperature bolometric microscopy was used to construct the local current mapping at the surface. These results indicate that the non-uniform regions on the surface disturb the current flow, and laser scanning images at room-temperature and at a low-temperature suggest a correlation between the structural properties and transport properties. Thus this method can be effective to evaluate the quality of the coated conductors.