• Journal of Food Hygiene and Safety
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• v.34 no.6
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• pp.557-561
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• 2019

This study investigated the efficacy of DNA extraction methods for real-time PCR detection of foodborne pathogenic bacteria in livestock manure composts. Livestock manure composts were inoculated with Escherichia coli O157:H7, Salmonella Typhimurium, Listeria monocytogenes, Bacillus cereus and incubated in enrichment broth. For DNA extraction, enriched samples were treated following boiling method, by chloroform, C₁₈ powder, and proteinase K. As a result, 4 species of bacteria were detected by real-time PCR when subjected to boiling for 30 min and treated with proteinase K. These results suggest that detection of foodborne pathogens by real-time PCR from livestock manure composts could be applicable using effective DNA extraction methodology such as the boiling method or proteinase K.

• Journal of Animal Environmental Science
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• v.9 no.1
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• pp.1-8
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• 2003

This study was conducted to calculate the amount of $N_2O$ emission from livestock manure management in Korea. $N_2O$ is considered a greenhouse gas emitted from livestock manure treatment. In order to calculate $N_2O$ emission, a percentage of nitrogen from livestock manure, livestock manure treatment facilities, and the number of livestock were collected. The amount of annual N excretion from beef cattle, dairy cattle, pigs, laying hen, and broiler were 37.00, 20.42, 12.37, 0.56, and 0.29kg, respectively Calculated $N_2O$ emission in 1990, 2005, 2010, 2015, and 2020 were 3.71, 5.84, 6.07, 6.23, and 6.53Gg, respectively. Increased $N_2O$ percentage in 2005, 2010, 2015, and 2020 compared to 1990 were 57.4, 63.6, 67.9, and 76.0%, respectively.

• Journal of Animal Science and Technology
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• v.60 no.2
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• pp.3.1-3.9
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• 2018

Background: Poultry breeding has increased by 306% in Korea, inevitably increasing the production of manure which may contribute to environmental pollution. The nutrients (NP) in the manure are essential for crop cultivation and soil fertility when applied as compost. Excess nutrients from manure can be accumulated on the land and can lead to eutrophication. Therefore, a nutrient load on the finite land should be calculated. Methods: This study calculates the nutrient production from Korean poultry by investigating 11 broiler and 16 laying hen farms. The broiler manure was composted using deep litter composting while for layer deep litter composting, drying, and simple static pile were in practice. The effect of weight reduction and storing period during composting was checked. Three weight reduction cases of compost were constructed to calculate nutrient loading coefficients (NLCs) using data from; i) farm investigation, ii) theoretical P changes (${\Delta}P=0$), and iii) dry basis. Results: During farm investigation of broiler and layer with deep litter composting, there was a 68 and 21% N loss whereas 77 and 33% P loss was found, respectively. In case of layer composting, a loss of 10-56% N and a 52% P loss was observed. Drying manure increased the P concentrations therefore NLCs calculated using dry basis that showed quite higher reductions (67% N; 53% P). Nutrient loss from farm investigation was much higher than reported by Korean Ministry of Environment (ME). Conclusions: Nutrients in manure are decreased when undergo storing or composting process due to microbial action, drying, and leaching. The nutrient load applied to soil is less than the fresh manure, hence the livestock manure management and conservation of environment would be facilitated.

• Journal of Applied Biological Chemistry
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• v.50 no.1
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• pp.22-28
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• 2007

Most of the uplands in alpine regions during off-season are left as bare soil and thus vulnerable to severe erosion due to the inherent topographical conditions. Appropriate management strategy to cope with this problem is urgently needed, yet few researches have been reported on the effects of winter cover crop and management on soil erosion. We assessed effects of ryegrass (Lolium multiflorum) as cover crop, green manure or mulching residue on soil erosion and quality through field and segment plot lysimeter experiments in alpine uplands. Ryegrass successfully adopted to winter in alpine region based on biomass, nutrient contents, and vigors of top and root systems. Incorporation of ryegrass into soil maintained soil fertility, nutrient uptake, and yield of cabbage exerting potential use as green manure. Cultivation of ryegrass suppressed occurrence of Chinese cabbage pests. Surface coverage by ryegrass as cover crop and mulching residue significantly reduced soil loss up to 96%, when combined with soil conservation management practices. Results revealed maintaining cover crop over winter was beneficial in reducing soil erosion, and sustaining soil quality and Chinese cabbage productivity. This study suggested winter cover crop, followed by green manure and mulching, and conservation tillage system could be one of the best management practices in alpine sloping uplands cultivating Chinese cabbage.

• Journal of Korean Society on Water Environment
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• v.31 no.3
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• pp.235-240
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• 2015

To evaluate the unit load generation and discharge, pig cage test was conducted. Feed intake, drink amount, and urine generation increased growth stage (heavy weight) of the pig more great. However, the sum of the urine and manure did not show a significant difference in the growth stages of pigs. Because of the limit of the experiment, e.g., research period, high pigpen temperature, breed-related stress and etc., it could not be derived the results of the four seasons. Therefore, in order to generalize the results, the feed intakes were calibrated using a NRC (National Research and nutritional requirements of pigs from the Commission) standards. The finalized unit load generation and generation amounts of manure and urine were estimated at BOD 104.1 g/head/d, T-N 21.2 g/head/d, T-P 4.9 g/head/d, manure 0.96 L/d, urine 1.66 L/d with consideration of revised feed intake. Compare to the former research results of MOE (Ministry of Environment, 1999) and NIAS (National Institute of Animal Science, 2008), the generation amounts of manure and urine were similar to the NIAS's values. In case of unit load generation, BOD and T-N were almost similar in all of them. However, the T-P unit load generation of MOE was more difference, e.g., 2.5 times high, compare to this study.

• Korean Journal of Soil Science and Fertilizer
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• v.34 no.4
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• pp.265-272
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• 2001

A manure compost has been identified as an alternative to fertilizer to increase soil fertility and crop production in farming fields. The aim of the present study was to evaluate the effects of pig manure compost on soil properties and rice productivity as well as to determine the optimum application rate. In 1997, a field experiment was carried out to evaluate the growth of rice on sandy loam, loam, and clay loam soils amended with 0, 5, 10, and $20Mg\;ha^{-1}$ of pig manure compost plus NPK, which decided by soil testing. Rice yields were higher in soils receiving manure compost amendment. The maxim um yields were evaluated with $7,520kg\;ha^{-1}$ in the levels of $4.2Mg\;ha^{-1}$ of pig manure compost application plus NPK in sandy loam, $7,320kg\;ha^{-1}$ in the levels of $10.7Mg\;ha^{-1}$ in loam, and $6,320kg\;ha^{-1}$ in $17.2Mg\;ha^{-1}$ in clay loam soil. The optimum application rate of pig manure compost, which decided for 95% of maximum yields, was $4.0Mg\;ha^{-1}$ in sandy loam and $7.0Mg\;ha^{-1}$ in loam and clay loam soils under the condition of chemical fertilization by soil diagnosis. An increase in rice yield indicated a better nutrient status in compost-amended soil which was supported by the higher nutrient contents of N, P and K in shoot of plants grown in soil with manure compost amendment. Addition of manure compost increased available phosphate, silicate and exchangeable K in the amended soils according to the rate of compost application rate. It can be concluded that the manure compost could be a suitable organic fertilizer for improving rice productivity and soil fertility, and an application rate of $4.0Mg\;ha^{-1}$ in sandy loam and $7.0Mg\;ha^{-1}$ in loam and clay loam soils would give the optimum rice yields in the standard fertilization by chemical fertilization.

• Korean Journal of Environmental Agriculture
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• v.27 no.4
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• pp.368-372
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• 2008

Composting is a good strategy for management of livestock manure. However, it leads to large ammonia emissions and has a potential phosphorus runoff due to high content of soluble phosphorus. The objective of this study was to evaluate the efficiency of alum on reducing ammonia emissions and stabilizing phosphorus during composting of pig manure. For this study, alum was applied at rates of 0 (No-Alum), 1.0 (Alum-L), and 3.0 (Alum-H) g Al $kg^{-1}$ pig manure and sawdust mixture (fresh matter basis). The thermophilic stage was quickly achieved in Alum-L and No-alum treatment, but it was delayed to 5 days in Alum-H treatment. The thermophilic stage was maintained for 2 weeks in all treatment. The pH of compost treated with alum remained below 8.0 for the 35 d but it was above 8.0 in No-Alum treatment. For the first 15 days of composting process, 93, 87, and 58% of total ammonia emissions were occurred in No-Alum-L and Alum-H, respectively. The Alum-H and Alum-L treatments reduced $NH_3$ volatilization by 31 and 78% compared with No-Alum treatment. Alum treatments shifted manure P form $H_2O$ and $NaHCO_3$ extractable P into NaOH extractable P which is very stable under acid and alkaline condition. Therefore, alum is a good chemical amendment for reducing ammonia emission during composting and potential losses of P following compost applications.

• Journal of The Korean Society of Agricultural Engineers
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• v.58 no.6
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• pp.31-38
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• 2016

The purpose of this study was to assess nonpoint sources (NPS) pollution affected by liquid manure and regulating gate in a small agricultural watershed. The study area, which is a wide plain farmland, was operating by the Buyong regulating gate in order to maintain irrigation water level during irrigation period. Consequentially, runoff only occurs through the gate at each event in rainy season for avoiding farmland inundation. In addition, the usage ratio of liquid manure in the study area has been increased greatly since 2014. Discharge loads at the Hwaingsan bridge subwatershed were 1.2 times for T-N, 4-10 times for T-P, and 3-8 times for TOC compared with the Soyang watershed (control) during study period. The reason was that NPS pollutants from upper Gpeun and Sangri bridge subwatersheds, which are widely spraying with livestock liquid manure, were stack at this subwaterehd because of regulating gate in non-rainy seasons. A number of agricultural watersheds in Saemangeum watershed are affected by regulating gate and vigorous livestock activities so that substantial management schemes under controling regulating gate are needed for minimizing livestock related NPS.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 1996.06c
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• pp.701-710
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• 1996

Composting has gained rapid acceptance as a method of recyling relatively dry organic materials such as leaves and brush and , when alternative disposal costs are high, even moist materials such as grass clippings and dewatered sewage sludges. However, as moisture contents rise above 60% , the need for a dry bulking amendment increase the costs of composting , both by direct purchases of amendment and though increased reactor capacity and materials handling requirements. High moisture materials also present increased risks of anaerobic odor formation through reduced oxygen transport (Miller , 1991) . These costs and operational challengers often constrain the opportunities to compost high moisture materials such as agricultural manures. During the last several decades economies of scale in livestock production have been increasing livestock densities and creating manure management challenges throughout the world. This issue is particularly pressing in Korea, where livestock arms typically manage little or no cropland, and the nutrients and boichemical oxygen demand in manure pose a serious threat to water quality. Composting has recently become popular as a means of recycling manure into products for sale off the farm, but bulking amendments (usually sawdust) are expensive designed to minimize bulking agent requirements by using the energy liberated by decompostion. In this context the composting reactor is used as a biological dryer, allowing the repeated use of bulking amendment with several batches of manure.

• Korean Journal of Organic Agriculture
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• v.19 no.spc
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• pp.153-156
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• 2011

Supplying rate of nitrogen at HV was 172.8 kg $ha^{-1}$, HV/B was 64.3 kg $ha^{-1}$ and B was 38.6 kg $ha^{-1}$. The Rice yield was 7.05 ton $ha^{-1}$ when the nitrogen supply was the largest with HV and 5.42 ton $ha^{-1}$ was produced on HV/B. The chemical characteristics of soil have lower pH and exchangeable cations(Ca and Mg) at B, HV and HV/B, rather than at CF because green manure was applied at the former step. However, the physical characteristics of the soil and the porosity showed different tendency which was that it was better at the green manure crops than CF. Nitrogen nutrient balance was showed the most balanced at CF and field of application of green manure crops were required the appropriate management if future crops would be cultivated because nitrogen nutrient could be exhausted or accumulated.