• 한국식품저장유통학회지
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• 제22권4호
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• pp.577-584
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• 2015

본 연구에서는 수입산 열대 아열대 과일의 항산화 효과를 비교하기 위하여 총 폴리페놀 함량, DPPH radical 소거능, ABTS radical 소거능, superoxide anion radical 소거능, FRAP 활성, tyrosinase 저해효과, 환원력 등을 측정하였다. 총 폴리페놀 함량은 석류가 12.22 mg GAE/g으로 가장 높았고 그 다음 순으로 파인애플이 3.77 mg GAE/g이었으며, 용과, 그린키위, 파파야, 옐로우망고가 2.45~2.99 mg GAE/g의 범위로 나타났다. 석류는 DPPH, ABTS, superoxide anion radical 소거능, 환원력에서 다른 과일보다 높은 활성을 보여주었고 그 다음으로 파인애플이 높은 활성을 보였다. 특히 석류는 추출물의 농도가 증가함에 따라 전반적으로 활성이 증가하는 경향을 보였고 시료 농도 5 mg/mL에서는 DPPH radical 소거능과 ABTS radical 소거능 및 환원력이 양성대조군으로 사용한 ascorbic acid와 대등한 효과를 보였다. 반면에, 용과와 파파야는 대부분의 항산화 효과 실험에서 낮은 활성을 나타내었다.

• 한국식품영양학회지
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• 제29권6호
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• pp.1015-1022
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• 2016

The purpose of this study was to investigate ash and mineral contents of agricultural products that are widely and specifically grown in Korea, including 23 fruits, 11 cereals and specialty crops, and 11 leaves and vegetables. Pre-treatment of mineral was performed by microwave method. Mineral contents were analyzed by Inductively Coupled Plasma-Optical Emission Spectrometer (ICP-OES) and Inductively Coupled Plasma Spectrophotometry Mass (ICP-MS). Ash contents ranged from 0.20 to 0.69 g/100 g in fruits, 1.62 to 3.80 g/100 g in cereals, and 0.28 to 2.93 g/100 g in leaves and vegetables. Among the 45 samples, the highest contents of ash were found in quinoa (average 3.80 g/100 g), the highest contents of calcium (Ca) were found in Hansan ramie leaves (average 894.79 mg/100 g), the highest contents of phosphorus (P) were found in yellow amaranth(average 661.88 mg/100 g), the highest contents of potassium (K) were found in quinoa (average 1,455.38 mg/100 g), the highest contents of magnesium (Mg) were found in red amaranth (average 434.02 mg/100 g), the highest contents of molybdenum (Mo) were found in moringa (average $482.50{\mu}g/100g$), and the highest contents of selenium (Se) were found in apple mango(average $23.67{\mu}g/100g$).

• Journal of Crop Science and Biotechnology
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• 제10권3호
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• pp.167-174
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• 2007

Spongy Alphonso mangoes were found to be infected with Staphylococcus bacteria. A Gram positive Staphylococcus strain was isolated from spongy pulp and identified from CABI Bioscience, UK, by partial 16S rDNA sequence analysis and by morphological and biochemical characterization through IMTECH, Chandigarh, India. Although identification by both of these methods indicated the organism belonged to same genus, different species names were given. Changes in total phenolics, reducing, and non-reducing sugars, respiration rate, total carotenoids, peroxidase(POX), and catalase activities were monitored during ripening of these fruits. The climacteric rise in spongy fruits was marked by an increase in respiration rate and a decrease in sugar content. Total phenolics content increased in spongy fruits as compared to ripe non-spongy fruits. Development of corky white tissue in spongy fruits was associated with about a 2.5-fold reduction in total carotenoids and a concomitant increase in lipoxygenase-mediated, $\beta$-carotene co-oxidation. A marked decrease in soluble protein content and about a 1.5-fold increase in POX activity was observed. Maximum POX activity was confined to 50-70%$(NH_4)_2SO_4$ fraction. The intense dark bands visible after POX specific substrate staining of the Native gel indicated a high expression of isoenzymes of POX in spongy fruits. Similarly, changes in levels of catalase activity were also observed in spongy fruits. The results suggest that infection of Alphonso mangoes with Staphylococcus bacteria affects the normal ripening processes of the fruit interfering with the carbohydrate and carotenoid metabolism. Also, the studies indicate the expression of POX and catalase enzymes as a plant defense response to microbial invasion.

• 한국식생활문화학회지
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• 제29권6호
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• pp.659-667
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• 2014

Fruits are good sources of vitamins, minerals, fiber, and phytochemicals, which are known to reduce serum lipids, oxidative damage, and blood pressure as well as improve blood glucose control. The purpose of this study was to estimate nutrient quality indices of fruits by carrying out a critical analysis of pre-existing methods according to their nutritional compositions. Four methods were used to assess the nutrient indices of 26 fruits, which are frequently consumed by Koreans based on the fourth Korean National Health and Nutrition Examination Survey (2009). Naturally nutrient rich score (NNR), nutrient rich food (NRF), nutrient adequacy score (NAS), and nutrient density score (NDS) were used to calculate nutrient quality indices. The Korean Nutrition Society Food Composition database of fruits based on 100 g edible portions was used. The algorithm of each method included the mean percentage of daily values (Dietary Reference Intakes for Koreans, 2010) for particular nutrients based on consumption of 1,900 kcal/day. The relative score indicated that strawberries, kumquat, and lemon had high nutrient quality indices. In addition, mango, lemon, persimmon, strawberry, apricot, and tangerine fruits are rich in antioxidant nutrients such as ${\beta}$-carotene, vitamin C, vitamin E, and selenium. However, scores of nutrient quality indices did not imply that higher scores of particular fruits are superior. We suggest moderate seasonable consumption a variety of fruits. Our results can be used as a reference for consumers when they choose fruits.

• 한국잡초학회지
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• 제14권1호
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• pp.71-80
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• 1994

Nepal, with its unique geographical and ecological features due to its abrupt rise in altitude, plays significant role in biological evolution. Existence of numerous wild relatives of the present-day cultivated agricultural crop plants in this small Himalayan nation may serve as a potential source of several yet unidentified desirable genes that are needed for future incorporation in the improvement of cultivated crop plants. This report includes 82 different wild relatives of 41 genera under 19 families of 37 agricultural crops of Nepal(Table 1). It serves as the sample of the glossary of these wild relatives of crop plants in Nepal. Under food grain crop plants of gramineae, leguminoceae and polygonaceae families, 16 different wild species namely wild rices(7 species), wild relatives of wheat plant(3 species), wild arhar(3 species), wild fingermillets(1 species) and wild buckwheat(2 species) have been identified in different parts of the country. Similarly, under vegetable crop plants of Araceae, Amaranthaceae, Crucifereae, Cucurbitaceae, Dioscoreaceae, Labiteae, Leguminosae, Liliaceae, Malvaceae, Polygonaceae, Solanaceae and Umbellifereae, 37 different wild species-wild colocasia(1 species), wild amaranths(3 species), wild leafy vegetables(2 species), wild gourds(3 species), wild cucumber(1 species), wild yams(4 species), wild mints(3 species), wild fenugreeks(4 species), wild pea(1 species), wild beans(3 species), wild garlics(2 species), wild spinach(3 species), wild lady's finger(1 species), wild spinach(3 species), wild eggplants(2 species) and wild carrot(one species) have also been identified. In case of wild relatives of cultivated orchard plants, 11 different wild species namely wild mango(one species), wild banana(one species), wild strawberry(one species), wild pear(one species), wild cherries(2 species), wild apple(one species) and wild grapes(3 species) have been identified, Among 19 different wild species of economic crop plants, five wild species of sugarcane, one species of wild sunhemp, two wild relatives of cotton, three wild relatives of rose, two wild species of tobacco, four wild species of turmeric and two wild species of tea have also been identified. This report includes only sample of the total wild species of the present-day cultivated agricultural crop plants. Further exploration on this economic botany will help the country in cataloging the wild relatives of cultivated crop plants and their future use in crop improvement.

• 한국응용곤충학회지
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• 제36권1호
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• pp.43-47
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• 1997

담팔수(Elaeocarpus sylvestris va. ellipticus Hara)를 기주로 하여 담팔수깍지벌레(Protopulvinaria mangiferae (Green))의 발육속도 및 증식능력을 조사하였다. 본 깍지벌레의 1령약충기부터 산란전성충기까지 발육소요일수는 온도가 상승할수록 짧았으며, 발육한계온도는 11.7$^{\circ}C$, 유효적산온도는 1000.0일도 이었다. $25^{\circ}C$, $30^{\circ}C$ 항온, 60-70% RH조건하에서 부화율은 각 온도구 모두 99% 이상으로 높았다. 산란전성층까지의 생존율은 $25^{\circ}C$에서 82%, $30^{\circ}C$에서 60%이었으며, 1령약충(neonate crawler)기의 사망률이 가장 높았다. 성충체로부터 1령약충의 이탈기간은 $25^{\circ}C$에서 50일 $30^{\circ}C$에서 33일간 이었으며, 이 기간 중 총이탈약충수의 50%이상이 분산기간 초기에 발생되었다. 발육기간 및 영별생존율 및 출생수를 이용하여 산출한 순번식율(${R}_{o}$), 평균세대기(T) 및 내적자연증가율은(${r}_{m}$)은 $25^{\circ}C$에서 각 132.6, 76.2, 0.064/암컷/일로 $30^{\circ}C$구 보다 높았다.

• Mycobiology
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• 제45권4호
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• pp.401-408
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• 2017

Colletotrichum gloeosporioides is an economically important fungal pathogen causing substantial yield losses indifferent host plants. To understand the genetic diversity and molecular epidemiology of this fungus, we have developed a novel, high-resolution multi-locus microsatellite typing (MLMT) method. Bioinformatic analysis of C. gloeosporioides unannotated genome sequence yielded eight potential microsatellite loci, of which five, CG1 $(GT)_n$, CG2 $(GT1)_n$, CG3 $(TC)_n$, CG4 $(CT)_n$, and CG5 $(CT1)_n$ were selected for further study based on their universal amplification potential, reproducibility, and repeat number polymorphism. The selected microsatellites were used to analyze 31 strains of C. gloeosporioides isolated from 20 different host plants from India. All microsatellite loci were found to be polymorphic, and the approximate fragment sizes of microsatellite loci CG1, CG2, CG3, CG4, and CG5 were in ranges of 213-241, 197-227, 231-265, 209-275, and 132-188, respectively. Among the 31 isolates, 55 different genotypes were identified. The Simpson's index of diversity (D) values for the individual locus ranged from 0.79 to 0.92, with the D value of all combined five microsatellite loci being 0.99. Microsatellite data analysis revealed that isolates from Ocimum sanctum, Capsicum annuum (chili pepper), and Mangifera indica (mango) formed distinct clusters, therefore exhibited some level of correlation between certain genotypes and host. The developed MLMT method would be a powerful tool for studying the genetic diversity and any possible genotype-host correlation in C. gloeosporioides.

• The Plant Pathology Journal
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• 제37권5호
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• pp.465-475
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• 2021

The aim of this study was to characterize potential fungal species affecting mangrove species in Mexico. The phytopathogens were identified based on morphological and molecular characteristics using internal transcribed spacer (ITS1/ITS4) primers then sequenced and compared with the other related sequences in GenBank (NCBI). Three fungal species were identified as Colletotrichum queenslandicum (Weir and Johnst, 2012) from black mangrove (Avicennia germinans); Colletotrichum ti (Weir and Johnst, 2012) from white mangrove (Laguncularia racemosa) and buttonwood mangrove (Conocarpus erectus); Fusarium equiseti (Corda) from red mangrove (Rhizophora mangle). In addition, C. ti and F. equiseti were identified from mango Mangifera indica L. sampled close by the mangrove area. This study provides first evidence of anthracnose on four mangrove species caused by Colletotrichum and Fusarium species in the "Términos" coastal lagoon in Campeche State southern Mexico. This is the first time that C. queenslandicum and C. ti are reported in Mexico. F. equiseti has not been reported affecting M. indica and R. mangle until the present work. Little is known regarding fungal diseases affecting mangroves in Mexico. These ecosystems are protected by Mexican laws and may be threatened by these pathogenic fungus. This is the first report of the effect of Trichoderma harzianum TRICHO-SIN as an effective biological control against of Colletotrichum and Fusarium species.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제15권10호
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• pp.3708-3728
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• 2021

Indian herbal plants are used in agriculture and in the food, cosmetics, and pharmaceutical industries. Laboratory-based tests are routinely used to identify and classify similar herb species by analyzing their internal cell structures. In this paper, we have applied computer vision techniques to do the same. The original leaf image was preprocessed using the Chan-Vese active contour segmentation algorithm to efface the background from the image by setting the contraction bias as (v) -1 and smoothing factor (µ) as 0.5, and bringing the initial contour close to the image boundary. Thereafter the segmented grayscale image was fed to a leaky capacitance fired neuron model (LCFN), which differentiates between similar herbs by combining different groups of pixels in the leaf image. The LFCN's decay constant (f), decay constant (g) and threshold (h) parameters were empirically assigned as 0.7, 0.6 and h=18 to generate the 1D feature vector. The LCFN time sequence identified the internal leaf structure at different iterations. Our proposed framework was tested against newly collected herbal species of natural images, geometrically variant images in terms of size, orientation and position. The 1D sequence and shape features of aloe, betel, Indian borage, bittergourd, grape, insulin herb, guava, mango, nilavembu, nithiyakalyani, sweet basil and pomegranate were fed into the 5-fold Bayesian regularization neural network (BRNN), K-nearest neighbors (KNN), support vector machine (SVM), and ensemble classifier to obtain the highest classification accuracy of 91.19%.

• Asia-Pacific Journal of Atmospheric Sciences
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• 제54권4호
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• pp.587-598
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• 2018

The impacts of aerosol loading on surface precipitation from mid-latitude deep convective systems are examined using a bin microphysics model. For this, a precipitation case over north central Mongolia, which is a high-altitude inland region, on 21 August 2014 is simulated with aerosol number concentrations of 150, 300, 600, 1200, 2400, and $4800cm^{-3}$. The surface precipitation amount slightly decreases with increasing aerosol number concentration in the range of $150-600cm^{-3}$, while it notably increases in the range of $600-4800cm^{-3}$ (22% increase with eightfold aerosol loading). We attempt to explain why the surface precipitation amount increases with increasing aerosol number concentration in the range of $600-4800cm^{-3}$. A higher aerosol number concentration results in more drops of small sizes. More drops of small sizes grow through condensation while being transported upward and some of them freeze, thus increasing the mass content of ice crystals. The increased ice crystal mass content leads to an increase in the mass content of small-sized snow particles largely through deposition, and the increased mass content of small-sized snow particles leads to an increase in the mass content of large-sized snow particles largely through riming. In addition, more drops of small sizes increase the mass content of supercooled drops, which also leads to an increase in the mass content of large-sized snow particles through riming. The increased mass content of large-sized snow particles resulting from these pathways contributes to a larger surface precipitation amount through melting and collision-coalescence.