• Nutrition Research and Practice
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• v.10 no.1
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• pp.11-18
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• 2016

BACKGROUND/OBJECTIVES: Type 2 diabetes (T2D) is more frequently diagnosed and is characterized by hyperglycemia and insulin resistance. $\small{D}$-xylose, a sucrase inhibitor, may be useful as a functional sugar complement to inhibit increases in blood glucose levels. The objective of this study was to investigate the anti-diabetic effects of $\small{D}$-xylose both in vitro and stretpozotocin (STZ)-nicotinamide (NA)-induced models in vivo. MATERIALS/METHODS: Wistar rats were divided into the following groups: (i) normal control; (ii) diabetic control; (iii) diabetic rats supplemented with a diet where 5% of the total sucrose content in the diet was replaced with $\small{D}$-xylose; and (iv) diabetic rats supplemented with a diet where 10% of the total sucrose content in the diet was replaced with $\small{D}$-xylose. These groups were maintained for two weeks. The effects of $\small{D}$-xylose on blood glucose levels were examined using oral glucose tolerance test, insulin secretion assays, histology of liver and pancreas tissues, and analysis of phosphoenolpyruvate carboxylase (PEPCK) expression in liver tissues of a STZ-NA-induced experimental rat model. Levels of glucose uptake and insulin secretion by differentiated C2C12 muscle cells and INS-1 pancreatic ${\beta}$-cells were analyzed. RESULTS: In vivo, $\small{D}$-xylose supplementation significantly reduced fasting serum glucose levels (P < 0.05), it slightly reduced the area under the glucose curve, and increased insulin levels compared to the diabetic controls. $\small{D}$-xylose supplementation enhanced the regeneration of pancreas tissue and improved the arrangement of hepatocytes compared to the diabetic controls. Lower levels of PEPCK were detected in the liver tissues of $\small{D}$-xylose-supplemented rats (P < 0.05). In vitro, both 2-NBDG uptake by C2C12 cells and insulin secretion by INS-1 cells were increased with $\small{D}$-xylose supplementation in a dose-dependent manner compared to treatment with glucose alone. CONCLUSIONS: In this study, $\small{D}$-xylose exerted anti-diabetic effects in vivo by regulating blood glucose levels via regeneration of damaged pancreas and liver tissues and regulation of PEPCK, a key rate-limiting enzyme in the process of gluconeogenesis. In vitro, $\small{D}$-xylose induced the uptake of glucose by muscle cells and the secretion of insulin cells by ${\beta}$-cells. These mechanistic insights will facilitate the development of highly effective strategy for T2D.

• Journal of the Korean Society of Marine Environment & Safety
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• v.30 no.3
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• pp.275-282
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• 2024

To ensure a systematic and integrated approach to defense safety management, individual safety management regulations have been consolidated into the National Defense Safety Directive. However, despite being enacted after the enforcement of the Severe Accident Punishment Act, the National Defense Safety Directive does not incorporate the contents of the Serious Accident Punishment Act. This omission is likely to cause confusion in safety management. In this paper, a PDCA analysis of the Severe Accident Punishment Act and the National Defense Safety Directive was conducted to identify area for improvement and supplementation in the Directive. Chapter 3 proposes amendments to clearly define the scope and responsibilities of safety management, implement serious accident prevention measures and inspections, and establish the penalties for those involved. These amendments aim to ensure faithful compliance with the Severe Accident Punishment Act. Chapter 4 emphasizes the implementation and inspection of risk assessments to enhance the effectiveness of safety accident prevention and preparation, thereby ensuring the completeness of the PDCA cycle.

• Journal of the Korean Applied Science and Technology
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• v.36 no.3
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• pp.942-953
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• 2019

The objective of this study was to evaluate the effects of L-arginine supplementation on muscle damage and fatigue indices and athletic performance improvement of canoe athletes after conducting a high-intensity training program. To achieve the objective, this study applied a high-intensity training program to seven high school canoe athletes. The high-intensity training program is composed of aerobic exercise sessions (twice per week; Tuesday and Thursday), anaerobic exercise sessions (three times per week; Monday, Wednesday, and Friday), and flexibility exercise sessions (five times per week). During the 6 week high-intensity training program, drug ingestion (L-arginine or placebo) was conducted in the first two weeks, wash out (two weeks) followed it, and drug ingestion (L-arginine or placebo) was carried out again in the last two weeks. The crossover design was used for the experiment so all study subjects were assigned to either the L-arginine intake group (the treatment group) or the placebo group (the control group). Each subject ingested 3g per day. This study confirmed the significant effects of L-arginine supplementation on muscle damage indices, fatigue indices, and antioxidants using blood samples. Additionally, FMD was analyzed to evaluate vascular endothelial cell functions and canoe performance was examined using the canoe ergometer. The results of this study showed that L-arginine intake did not have direct effects on the levels of ammonia, IP, and CK. The level of LDH decreased significantly more in the ARG group than in the PLA group due to L-arginine supplementation. Moreover, L-arginine supplementation did not change total NO, d-ROMs, BAP, and FMD significantly. Lastly, the results of the 500m canoe ergometer, which was conducted to evaluate the canoe performance, revealed that L-arginine did not have direct effects on total time, stroke distance, and mean velocity. However, L-arginine supplementation significantly improved muscle damage indices, fatigue indices, antioxidants, FMD, and canoe performance. Therefore, it is believed that additional studies are needed for examining the potential effects of L-arginine supplementation athletic performance enhancement.

• Asian-Australasian Journal of Animal Sciences
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• v.19 no.1
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• pp.35-41
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• 2006

The aim of this study was to investigate the effects of three herb supplementations on blood metabolites, hormones, antioxidant activity, immunoglobulin (Ig) G concentration, and ruminal fermentation in steers. Four Holstein steers in a $4{\times}4$ Latin square design received four herb treatments. The treatments consisted of the steers' regular diets with addition of: 1) nothing (control), 2) peppermint, 3) clove, and 4) lemongrass at 5% of the diet (DM basis). Clove supplementation increased the plasma concentration of cholesterol by about 10% (from 79 to 87 mg/dl). Peppermint and lemongrass feeding resulted in an increase in the concentrations of plasma urea nitrogen (from 5.9 to 6.9 and 6.4 mg/dl, respectively). The three herb treatments had no effect on other metabolites and hormones. Steers receiving clove supplementation showed a higher plasma antioxidant activity. The three herb treatments caused lower concentrations of IgG in the blood. Peppermint and lemongrass feedings increased, and clove feeding decreased ruminal concentrations of ammonia. There were no significant differences in VFA concentrations among herbal treatments, except for the decrease in propionate concentration in steers receiving clove treatment. This study suggested that clove feeding changed cholesterol metabolism and increased antioxidant activity in plasma, and feeding of three herbs affected immunity system and ruminal fermentation in steers.

• Asian-Australasian Journal of Animal Sciences
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• v.14 no.5
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• pp.631-639
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• 2001

Two experiments were carried out concerning the effects of urea-molasses cake (UMC) and its separate components as supplements on rumen environment, in sacco feed degradability and intake of swamp buffaloes fed rice straw, grasses or a mixture of grasses and rice straw. Experiment 1 was a change-over design with 4 animals and 6 treatments. The buffaloes were fed rice straw ad libitum, and the experimental treatments were: no supplementation (R); 700 g of the complete urea-molasses cake (RUMC); 53.2 g urea (RU); 276 g rice bran and 52.5 coconut meal (RRC); 26.6 g salt, 26.6 g bone meal and 2.1 g trace minerals (RMi); and 25 g molasses (RMo). Experiment 2 was a Latin square design with four diets and four animals. The treatments were: rice straw ad libitum and mixed grass (RG) at 2.5 g dry matter per kg live weight (LW); RG plus 700 g urea-molasses cake (RGUMC); mixed grass ad libitum (G); and G plus 700 g cake (GUMC). In both experiments the supplements were fed once daily. In Exp. 1 although the rumen pH was significantly different (p<0.05) among diets, it varied only from 6.90 to 7.06. The ruminal ammonia was also significantly (p<0.05) different among the diets with RUMC significantly higher than R. Total bacterial and protozoal counts were significantly (p<0.05) higher for the RUMC, RU, RMo and RRC diets. Total feed and rice straw intakes were highest for RUMC (p<0.05) and lowest for the RMi and RMo diets, but in sacco degradability of four different roughages were not significantly different among diets. In Exp. 2, rumen pHs of the diets differed significantly and (p<0.01) ranged from 7.04 - 7.19. Ruminal $NH_3-N$ concentrations (mg/100 ml) were also significantly different (p<0.05), and higher for the RGUMC, G and GUMC diets. The total counts of bacteria and protozoa were significantly (p<0.05) higher for the RGUMC, G and GUMC diets. The total feed intake and roughage intake were significantly (p<0.05) higher for the RGUMC, G and GUMC diets compared to the RG diet. Correspondingly, LW changes also differed among treatments (p=0.06). It was concluded that there were significant increases in rumen $NH_3-N$ concentration, microbial populations and feed intake in the buffaloes by UMC supplementation, whereas the significant difference in in sacco DM degradation was not found by any type of supplementation. There seemed to be a need of a combination of urea, molasses, minerals and other protein nitrogen sources to enhance rice straw intake. Adding grass to the rice straw diet at 0.25% LW (DM) should also be considered to maintain buffalo rumen function and production with UMC supplementation, when rice straw is the main roughage.

• Journal of Environmental Science International
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• v.23 no.8
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• pp.1429-1436
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• 2014

In this study, we investigated the effects of dietary supplementation (n = 40 pigs/treatment) with bamboo powder (0, 1, 2 and 3%) for 38 days. We evaluated growth performance, blood metabolites, and carcass characteristics of fattening pigs and gas emission and microbial populations in pig manure, to obtain data on pork producers for environmental management. We obtained the following results. First, supplementation with increasing amounts of bamboo powder had a significant (P < 0.05) effect on feed intake, feed efficiency, and glucose contents (except for initial and final body weight, weight gain, carcass characteristics, and blood urea nitrogen). In terms of blood metabolites, glucose and blood urea nitrogen tended to decrease with increasing amounts of bamboo powder. Second, the amounts of ammonia, methane, amine, hydrogen sulfide, and acetic acid were reduced by increasing amounts of bamboo powder when compared with the controls (P < 0.05). However, there were no significant differences in pH, propionic acid, iso-butyric acid, butyric acid, iso-valeric acid, and valeric acid among all treatments. The lowest gas emission was observed when 3% bamboo powder was used. Third, supplementation with increasing amounts of bamboo powder tended (P < 0.05) to increase the total number of bacteria, Lactobacillus spp., and yeast, but E. coli, Salmonella spp., and Shigella spp. were not detected in any treatment. In conclusion, the results of this study suggest that supplementation with bamboo powder was effective in reducing gas emission and inhibiting pathogen populations in pig manure by lowering the pH of the manure.

• Asian-Australasian Journal of Animal Sciences
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• v.18 no.9
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• pp.1299-1304
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• 2005

An experiment was conducted to study the production performance of broiler breeder females (25 to 40 weeks of age) fed either reference diet or low non-phytate phosphorus (NPP) diet with or without microbial phytase (500 FYT/kg) supplementation. A weighed (160 g/b/d) quantity of feed from each diet was offered daily to 40 replicates of one bird each housed in California type cage having individual feeders. Each cage was considered as a replicate. A continuous 16-h light per day was provided using incandescent bulbs. Body weight, egg production, egg weight, feed per egg mass, egg specific gravity, egg breaking strength, shell thickness, tibia ash and serum Ca and protein concentrations were not affected by reducing the NPP level from 0.30 to 0.18% in the broiler breeder diet. Supplementation of phytase (500 FYT/kg) enzyme to the diet containing 0.18% NPP had no added advantage on any of the above production parameters. The serum inorganic P was increased significantly (p<0.05) by either enhancing the NPP content from 0.18 to 0.30% or supplementing phytase @500 FYT/kg to the diet containing low P which were found comparable. Retention of Ca and P was positive on all the diets. P retention decreased significantly (p<0.05) with either increase in NPP content or phytase supplementation in the diet. Neither NPP nor phytase supplementation influenced bone mineralization in terms of tibia ash and strength. The hatchability was not influenced by either increasing the NPP content or supplementing the enzyme phytase. Similarly, the P concentration in the egg yolk and day old chick, day old and 14th day body weight and leg score was not altered by increasing the level of NPP or supplementing phytase enzyme. The mortality was within the normal limits in all the three dietary groups. Thus, it can be concluded that 0.18% NPP (288 mg NPP intake/b/d) in the broiler breeder' diet is adequate in sustaining the optimum performance from 25 to 40 wks of age. Enhancing the NPP content or supplementation of phytase (500 FYT/kg diet) to diet containing 0.18% NPP had no added advantage on performance.

• Asian-Australasian Journal of Animal Sciences
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• v.16 no.11
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• pp.1614-1618
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• 2003

Twenty crossbred (HF${\times}$Tharparkar) dry pregnant cows were divided into four equal groups. They were supplemented with 1,000 I.U. $\alpha$-tocopheryl acetate from 0 (group I), 15 (group II), 30 (group III) and 60 (group IV) days before parturition to 1month of lactation. All the cows were kept under similar feeding and management conditions. Blood plasma samples collected on specific days were analyzed for $\alpha$-tocopherol, retinol, total antioxidant activity (FRAP), immunoglobulin and calcium. Plasma $\alpha$-tocopherol concentration at 30 days prepartum averaged 3.5, 4.1, 4.4 and $3.9{\mu}g/ml$ and decreased by 50.0, 41.4, 34.1 and 33.3 percent on the day of parturition in the four respective groups. After calving, plasma vitamin E started to recover earlier in groups II, III and IV as compared to group I. Mean plasma $\alpha$-tocopherol concentration at 21 days postpartum was significantly higher in groups II, III and IV (2.9, 3.5 and $3.1{\mu}g/ml$) compared to group I ($1.9{\mu}g/ml$) cows. Plasma retinol concentration also showed a substantial decrease in all the groups on the day of calving but recovered to its normal value at 3 weeks postpartum. Plasma total antioxidant activity averaged 901, 895, 859 and $875{\mu}mol/l$ in the four respective groups on 30 days prepartum and decreased on the day of calving in all the groups, but the decrease was less in groups III and IV. Plasma immunoglobulin concentration was higher in group IV, followed by groups III, II and I, respectively, showing better immune status of vitamin E supplemented cows due to less oxidative stress. Supplementation of vitamin E resulted in higher plasma calcium concentration. The data showed that vitamin E supplementation should be started at least 30 days prepartum to reduce oxidative stress in periparturient cows.

• Asian-Australasian Journal of Animal Sciences
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• v.7 no.4
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• pp.563-570
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• 1994

Fifty-one Thai Native (TN) and Anglo-Nubian (AN) $\times$ TN does were studied. The purpose of the study was to investigate the reproductive performances of different goat genotypes grazing improved pasture with or without supplementary feeding. The feeding regimes were: 1. no concentrate supplement (T1), 2. supplemented for 15 days before mating and 45 days during mating period (T2), 3. supplemented from 15 days before mating to 42 days after kidding (T3) and 4. supplemented for 30 days before kidding, followed by 42 days after kidding. Cross-bred does tended to have higher conception rates, kidding opportunities and higher multiple birth rates than TN does. However, these differences were not statistically significant (p>0.05), and concentrate supplementation under the various regimes did not increase reproductive performance. TN kids had significantly (p<0.01) lower birth weights and lower weights at 3, 6 and 12 weeks of age than those of the cross-bred kids. However, there was no significant difference between the genotypes in growth rate (g/d or $g/kg^{75}/d$) of kids during these periods. Supplementary feeding did not significantly affect either kid birth weight or weight gain in the first 6 weeks after birth and during this period supplementary adequate in both quantity and quality, substantial reproductive performances were achieved from both TN and AN $\times$ TN does without concentrate supplementation.

• Nutrition Research and Practice
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• v.9 no.4
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• pp.400-403
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• 2015

BACKGROUND/OBJECTIVES: By the year 2050, thirty-eight percent of the Korean population will be over the age of 65. Health care costs for Koreans over age 65 reached 15.4 trillion Korean won in 2011, accounting for a third of the total health care costs for the population. Chronic degenerative diseases, including coronary heart disease (CHD), drive long-term health care costs at an alarming annual rate. In the elderly population, loss of independence is one of the main reasons for this increase in health care costs. Korean heath policies place a high priority on the prevention of CHD because it is a major cause of morbidity and mortality. SUBJECTS/METHODS: This evidence-based study aims to the estimate potential health care cost savings resulting from the daily intake of omega-3 fatty acid supplementation. Potential cost savings associated with a reduced risk of CHD and the medical costs potentially avoided through risk reduction, including hospitalizations and physician services, were estimated using a Congressional Budget Office cost accounting methodology. RESULTS: The estimate of the seven-year (2005-2011) net savings in medical costs resulting from a reduction in the incidence of CHD among the elderly population through the daily use of omega-3 fatty acids was approximately 210 billion Korean won. Approximately 92,997 hospitalizations due to CHD could be avoided over the seven years. CONCLUSIONS: Our findings suggest that omega-3 supplementation in older individuals may yield substantial cost-savings by reducing the risk of CHD. It should be noted that additional health and cost benefits need to be revisited and re-evaluated as more is known about possible data sources or as new data become available.