Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
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Effects of l-arginine supplementation with high-intensity training on muscle damage and fatigue index and athletic performance in Canoe Athletes

L-arginine 섭취가 고강도 훈련 프로그램에 따른 카누선수의 근 손상 지표, 피로 물질 및 경기력 향상에 미치는 영향

  • 정종환 (대한카누연맹) ;
  • 강은범 (대전대학교 건강운동관리학과) ;
  • 김창환 (대전대학교 건강운동관리학과)
  • Received : 2019.07.29
  • Accepted : 2019.09.28
  • Published : 2019.09.30
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Abstract

The objective of this study was to evaluate the effects of L-arginine supplementation on muscle damage and fatigue indices and athletic performance improvement of canoe athletes after conducting a high-intensity training program. To achieve the objective, this study applied a high-intensity training program to seven high school canoe athletes. The high-intensity training program is composed of aerobic exercise sessions (twice per week; Tuesday and Thursday), anaerobic exercise sessions (three times per week; Monday, Wednesday, and Friday), and flexibility exercise sessions (five times per week). During the 6 week high-intensity training program, drug ingestion (L-arginine or placebo) was conducted in the first two weeks, wash out (two weeks) followed it, and drug ingestion (L-arginine or placebo) was carried out again in the last two weeks. The crossover design was used for the experiment so all study subjects were assigned to either the L-arginine intake group (the treatment group) or the placebo group (the control group). Each subject ingested 3g per day. This study confirmed the significant effects of L-arginine supplementation on muscle damage indices, fatigue indices, and antioxidants using blood samples. Additionally, FMD was analyzed to evaluate vascular endothelial cell functions and canoe performance was examined using the canoe ergometer. The results of this study showed that L-arginine intake did not have direct effects on the levels of ammonia, IP, and CK. The level of LDH decreased significantly more in the ARG group than in the PLA group due to L-arginine supplementation. Moreover, L-arginine supplementation did not change total NO, d-ROMs, BAP, and FMD significantly. Lastly, the results of the 500m canoe ergometer, which was conducted to evaluate the canoe performance, revealed that L-arginine did not have direct effects on total time, stroke distance, and mean velocity. However, L-arginine supplementation significantly improved muscle damage indices, fatigue indices, antioxidants, FMD, and canoe performance. Therefore, it is believed that additional studies are needed for examining the potential effects of L-arginine supplementation athletic performance enhancement.

본 연구는 L-arginine 섭취가 고강도 훈련 프로그램에 따른 카누선수의 근 손상 지표, 피로 물질 및 경기력 향상에 미치는 영향을 확인하는데 목적이 있다. 이를 위해 고등학교 카누 선수 7명을 대상으로 고강도 훈련 프로그램을 적용하였으며, 고강도 훈련 프로그램은 주 2회의 유산소 운동(화, 목), 주 3회의 무산소 운동(월, 수, 금) 그리고 주 5회의 유연성 운동을 실시하였다. 고강도 훈련 프로그램을 진행하는 6주 과정 중에 처음 2주는 약물섭취(L-arginine or 위약)를 실시하고 다음 2주는 wash out, 그리고 마지막 2주도 약물섭취(L-arginine or 위약)를 실시하였다. 모든 연구대상자들이 L-arginine 섭취(시험군)와 위약그룹(대조군)으로 배정되는 교차설계로 디자인하였다. L-arginine은 하루 총 3g으로 섭취하였다. 채혈을 통해 L-arginine 섭취에 따른 근 손상 지표, 피로 지표, 항산화력을 확인하였으며, 혈관내피세포기능 분석을 위해 FMD, 그리고 카누 에르고미터를 활용하여 카누 경기력을 분석하였다. 본 연구결과 L-arginine 섭취에 따른 Ammonia, IP, CK의 수준의 직접적인 효과는 나타나지 않았으며, LDH의 수준은 L-arginine 섭취로 인해 PLA 그룹보다 ARG 그룹에서 더 감소하는 것으로 나타났다. L-arginine 섭취에 따른 Total NO, d-ROMs, BAP, 그리고 FMD의 수준도 통계적으로 유의한 차이는 없는 것으로 나타났다. 마지막으로 카누 경기력 향상을 확인하기 위해 실시한 500M 카누 에르고미터 결과에서 총시간, 스트록 거리, 평균속도 분석에서 L-arginine의 운동수행능력 향상의 직접적인 효과는 나타나지 않았다. 하지만 L-arginine 섭취로 인해 근 손상 지표, 피로 지표, 항산화력, FMD, 그리고 카누 경기력 수준이 개선되는 경향은 나타났다. 따라서 L-arginine 섭취의 잠재적인 운동능력 향상 보조제 효과에 대한 추가적인 연구가 필요할 것으로 생각된다.

Keywords

References

  1. J. H. Jung, Y. E. Song, C. H. Kim, E. B. Kang, J. Y. Cho, "Effect of periodical complex training program on the body composition and erogometer record of national canoe athletes", The Korean Journal of Sport, Vol.11, No.3, pp. 75-84, (2013).
  2. K. Y. Hong, "The Effect of Training Patterns on the AT Improvement for Canoe Players", Korean journal of physical education, Vol.42, No.4, pp. 663-672. (2003).
  3. J. H. Yoon, Y. S. Ji, D. Y. Woo, "The change of blood lactic acid, LDH and CPK activity during arm ergometer exercise in canoeists", Journal of Korean Society for the Study of Physical Education, Vol.7, No.1, pp. 70-80. (2002).
  4. L. E. Armstrong, J. L. VanHeest, "The unknown mechanism of the overtraining syndrome: clues from depression and psychoneuroimmunology", Sports medicine, Vol.32, No.3, pp. 185-209. (2002). https://doi.org/10.2165/00007256-200232030-00003
  5. M. Tanskanen, M. Atalay, A. Uusitalo, "Altered oxidative stress in overtrained athletes", Journal of sports sciences, Vol.28, No.3, pp. 309-317. (2010). https://doi.org/10.1080/02640410903473844
  6. A. C. Snyder, H. Kuipers, B. Cheng, R. Servais, E. Fransen, "Overtraining following intensified training with normal muscle glycogen", Medicine and science in sports and exercise, Vol.27, No.7, pp. 1063-1070. (1995). https://doi.org/10.1249/00005768-199507000-00016
  7. J. B. Kreher, J. B. Schwartz, "Overtraining syndrome: a practical guide", Sports Health, Vol.4, No.2, pp. 128-138. (2012). https://doi.org/10.1177/1941738111434406
  8. M. Mohr, J. J. Nielsen, J. Bangsbo, "Caffeine intake improves intense intermittent exercise performance and reduces muscle interstitial potassium accumulation", Journal of applied physiology, Vol.111, No.5, pp. 1372-1379. (2011). https://doi.org/10.1152/japplphysiol.01028.2010
  9. Z. Shen, Z. Chen, Y. Lu, F. Chen, Z. Chen, "Relationship between gene expression of nitric oxide synthase and androgens in rat corpus cavernosum", Chinese medical journal, Vol.113, No.12, pp. 1092-1095, (2000).
  10. M. Burtscher, F. Brunner, M. Faulhaber, B. Hotter, R. Likar, "The prolonged intake of L-arginine-L-aspartate reduces blood lactate accumulation and oxygen consumption during submaximal exercise", Journal of sports science & medicine, Vol.4, No3, pp. 314-322, (2005).
  11. G. K. McConell, N. N. Huynh, R. S. Lee-Young, B. J. Canny, G. D. Wadley, "L-Arginine infusion increases glucose clearance during prolonged exercise in humans", American journal of physiology. Endocrinology and metabolism, Vol.290, No.1, pp. 60-66, (2006) https://doi.org/10.1152/ajpendo.00263.2005
  12. T. H. Liu, C. L. Wu, C. W. Chiang, Y. W. Lo, H. F. Tseng, C. K. Chang, "No effect of short-term arginine supplementation on nitric oxide production, metabolism and performance in intermittent exercise in athletes", The Journal of nutritional biochemistry, Vol.20, No.6, pp. 462-468. (2009). https://doi.org/10.1016/j.jnutbio.2008.05.005
  13. T. S. Alvares, C. A. Conte-Junior, J. T. Silva, V. M. Paschoalin, "L-arginine does not improve biochemical and hormonal response in trained runners after 4 weeks of supplementation", Nutrition research, Vol.34, No.1, pp. 31-39. (2014). https://doi.org/10.1016/j.nutres.2013.10.006
  14. S. H. Yang, H. K. Kwon, Y. S. Kwak, "The Effects of L-arginine and GPLC supplementation on plasma nitric oxide, energy substrates and fatigue factors in high school football players", Exercise Science, Vol.24, No.3, pp. 233-242, (2015). https://doi.org/10.15857/ksep.2015.24.3.233
  15. G. Borg, Borg's Perceived Exertion and Pain Scales, "Human Kinetics", (1998).
  16. R. Stanton, P. R. Reaburn, B. Humphries, "The effect of short-term Swiss ball training on core stability and running economy", Journal of strength and conditioning research, Vol.18, No.3, pp. 522-528, (2004). https://doi.org/10.1519/00124278-200408000-00023
  17. D. Martarelli, P. Pompei, "Oxidative stress and antioxidant changes during a 24-hours mountain bike endurance exercise in master athletes" The Journal of sports medicine and physical fitness, Vol.49, No.1, pp. 122-127, (2009)
  18. P. Brancaccio, N. Maffulli, R. Buonauro, F. M. Limongelli, "Serum enzyme monitoring in sports medicine", Clinics in sports medicine, Vol.27, No.1, pp. 1-18, (2018) https://doi.org/10.1016/j.csm.2007.09.005
  19. P. S. Bradley, M. Di Mascio, D. Peart, P. Olsen, B. Sheldon, "High-intensity activity profiles of elite soccer players at different performance levels", Journal of strength and conditioning research / National Strength & Conditioning Association, Vol.24, No.9, pp. 2343-2351. (2010) https://doi.org/10.1519/JSC.0b013e3181aeb1b3
  20. K. Nosaka, A. Aldayel, M. Jubeau, T. C. Chen, "Muscle damage induced by electrical stimulation", European journal of applied physiology, Vol.111, No.10, pp. 2427-2437, (2011). https://doi.org/10.1007/s00421-011-2086-x
  21. I. Y. Paik,, W. R. Chang, Y. S. Kwak, S. Y. Cho, H. E. Jin, "The Effect of Prunus Mume Supplementation on Energy Substrate Levels and Fatigue Induction Factors", Journal of Life Science, Vol.20, No.1, pp. 49-54. (2010). https://doi.org/10.5352/JLS.2010.20.1.049
  22. J. Bangsbo, P. D. Gollnick, T. E. Graham, C. Juel, B. Kiens, M. Mizuno, B. Saltin, "Anaerobic energy production and O2 deficit-debt relationship during exhaustive exercise in humans", The Journal of physiology, Vol. 422, pp. 539-559, (1990). https://doi.org/10.1113/jphysiol.1990.sp018000
  23. T. S. Alvares, C. M. Meirelles, Y. N. Bhambhani, V. M. Paschoalin, P. S. Gomes, "L-Arginine as a potential ergogenic aid in healthy subjects", Sports Medicine, Vol.41, No.3, pp. 233-248, (2011). https://doi.org/10.2165/11538590-000000000-00000
  24. S. Doutreleau, B. Mettauer, F. Piquard, A. Schaefer, E. Lonsdorfer, R. Richard, B. Geny, "Chronic but not acute oral L-arginine supplementation delays the ventilatory threshold during exercise in heart failure patients", Canadian journal of applied physiology, Vol.30, No.4, pp. 419-432, (2005). https://doi.org/10.1139/h05-131
  25. P. Tripathi, M. K. Misra, "Therapeutic role of L-arginine on free radical scavenging system in ischemic heart diseases", Indian journal of biochemistry & biophysics, Vol.46, No.6, pp. 498-502, (2009).
  26. S. M. Bode-Boger, J. Muke, A. Surdacki, C. Brabant, R. H. Boger, J. C. Frolich, "Oral L-arginine improves endothelial function in healthy individuals older than 70 years", Vascular medicine, Vol.8, No.2, pp. 77-81. (2003). https://doi.org/10.1191/1358863x03vm474oa
  27. W. Durante, F. K. Johnson, R. A. Johnson, "Arginase: a critical regulator of nitric oxide synthesis and vascular function", Clinical and experimental pharmacology & physiology, Vol.34, No.9, pp. 906-911, (2007). https://doi.org/10.1111/j.1440-1681.2007.04638.x
  28. M. Liang, Z. Wang, H. Li, L. Cai, J. Pan, H. He, Q. Wu, Y. Tang, J. Ma, L. Yang, "l-Arginine induces antioxidant response to prevent oxidative stress via stimulation of glutathione synthesis and activation of Nrf2 pathway", Food and chemical toxicology, Vol.115, pp. 315-328, (2018) https://doi.org/10.1016/j.fct.2018.03.029
  29. C. Goto, Y. Higashi, M. Kimura, K. Noma, K. Hara, K. Nakagawa, M. Kawamura, K. Chayama, M. Yoshizumi, I. Nara, "Effect of different intensities of exercise on endothelium-dependent vasodilation in humans role of endothelium-dependent nitric oxide and oxidative stress", Circulation, Vol.108, No.5, pp. 530-535, (2003) https://doi.org/10.1161/01.CIR.0000080893.55729.28
  30. A. Baudouin, D. Hawkins, "A biomechanical review of factors affecting rowing performance", British journal of sports medicine, Vol.36, No.6, pp. 396-402, (2002). https://doi.org/10.1136/bjsm.36.6.396
  31. G. K. Lee, H. Y. Kim, "The Effects of Complex Training on K-1 500m Record and Physical Fitness of Canoe Players", Korean Journal of Sports Science, Vol.27, No.1, pp. 619-628, (2018). https://doi.org/10.35159/kjss.2018.02.27.1.619
  32. J. G. Choi, Y. J. Kim, "The study on analysis of muscle fatigue and recruitment type in major muscles during Canoe ergometer exercise", Korea Sport Research, Vol.15, No.2, pp. 1601-1612, (2004).