• Preventive Nutrition and Food Science
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• 제5권1호
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• pp.7-9
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• 2000

Variouss concentrationss of free malonaldehyde were prepared from 1,1,3,3-tetraethoxy propane (TEP). Spectrophoto-metric determination and HPLC analysis of free malonaldehyde instead of malonaldehyde-thiobaribituric acid (MA-TBA) complex were conducted. Malonaldehyde was well separated on a $\mu$Bondapak C18 column. The absorbances at 254 nm and the HPLC peak areas of free malonaldehyde increased with the increase in its concentration. The correlation coefficient between absorbances and peak areas was 0.998. The total time elapsed to conduct the whole procedure was less than 15 minutes. This method directly measured the amount of free malonaldehyde in a short period of time successfully. This procedure is expected to be used as a rapid, accurate and specific means to de-termine the development of lipid oxidation in food.

• Journal of Ginseng Research
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• 제14권3호
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• pp.373-378
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• 1990

Free malonaldehyde was determined in nine kinds of ginseng polyacetylene-treated micro- some by HPLC analysis. Antioxidant activities of some phenolic compounds and ginseng saponin were also drtermined both by a new HVLC method and by THA method. A new HPLC system separaterl malonaldehyde at a retention time 5,6 min and showed a linear relationship between the peak are a and malonaldehyde concentration. Panaxnol showed the strongest activity among nine polyacetylenes and the addition of either chlorine or aletyl group reduced polyacetylene's own activity. Since C14-polyacetylenes such as panaxyne and panaxyne-epoxide had little or no antioxidant activities, S17-structure should be preserved to exert a radical-scvenging or trapping activity. The antioxidant activities of chlorogenic acid, ferulic acid and catechol were much weaker than those of C17-polyacetylenes. Ginseng saponin showed no antioxidant activity. Since TBA reactive substances and malonaldehyde contents were almost the same in peroxiedized microsome. TBA value seems a good indicator for lipid peroxidation in this particular Fe+3 ADP/NADPH system.

• 한국식품영양학회지
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• 제19권4호
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• pp.374-380
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• 2006

저염 굴비의 가공 방법을 모색하고자 염장 기간을 달리하여 식염 농도가 상이한 굴비의 건조 및 저장 중 지질 과산화물인 malonaldehyde의 함량 및 지방산의 조성 변화를 분석 비교하였다. 천일염으로 염장 기간을 5시간과 5일로 달리하여 천일건조와 저장을 거치는 동안 수분의 함량은 각 가공 단계 및 저장 기간의 경과와 더불어 점차 유의적으로 감소하는 경향을 나타내었다. 염도는 5시간 염장 시료와 5일 염장 시료간에 약 10배의 차이가 있었으며, 수분과 상반되게 건조와 저장 과정을 거치면서 점차로 증가하는 경향을 보였고, pH는 저장 기간에 따른 차이가 미미하였다. Malonaldehyde는 5시간 염장 시료에 비하여 5일 염장한 시료에서, 근육부분보다는 표피 부분에서 더 높은 함량으로 정량되었으며 가공 과정을 거치면서 계속적으로 증가하는 경향이었다 지방산은 oleic acid $(C_{18:1})$, palmitic acid$(C_{16:0})$, docosahexaenoic acid$(C_{22:6})$의 함유 비율이 높았으며 가공을 거치면서 포화 지방산은 증가하고 불포화 지방산은 감소하는 경향이었다. 저장 21일에 5시간 염장 시료와 5일 염장 시료의 불포화 지방산 잔존율은 각각 1.39와 0.99로 굴비 가공 중 불포화 지방산은 첨가 식염의 농도가 높을수록, 가공보다는 저장 동안에 급격히 변화함을 알 수 있었다.

• Journal of Nutrition and Health
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• 제13권3호
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• pp.145-149
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• 1980

To study the rancidity of dried yellow carlbina oil by oxidation during the storage period general properties of the oil and composition of its fatty acid were analysed quantitatively with gas chromatography The results indicated that I) The dried yellow carbina oil was involved in drying oil of high degree of unsaturation with IV 138 and consisted of higher-fatty acid with SV 194. 2) The composition of the fatty acids were composed of 18 fatty acids involving 6 unknown fatty acids and comprised poly-unsaturated fatty acid with $C_{18}^{:3}\:and\:C_{22}^{:5}$ 3) After three morths storage of dried yellow carbina the content of malonaldehyde was about 12mg/kg in its exterior part, but 6mg/kg in interior part, which indicateing that the degree of rancidity of poly unsaturated glycerides in exterior part of carbina were two times as much as that of interior part.

• 대한가정학회지
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• 제18권3호
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• pp.13-19
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• 1980

To study the rancidity of dried file fish oil by Oxidation during the storage period, general properties of the oil and composition of its fatty acids were analysed qumtitatively with gas-chromatography. The results indicated that; 1) The dried file fish oil was involved in drying oil of heigh degree of unsaturation with IV 158., and consisted of higher-fatty acid with SV 190. 2) The composition of the fatty acids were composed of 18 fatty acids involving 6 unknown fatty acids, and polyunsaturated fatty acid with docosapentaenoic acid(20 weight%). 3) Changes of malonaldehyde content during the storage of dried file fish were about 5.0 mg/kg after 10day, 3.4 mg/kg after 40 days.

• Bulletin of the Korean Chemical Society
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• 제24권8호
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• pp.1097-1101
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• 2003

The cubic and quartic anharmonic force field of malonaldehyde is calculated using density functional theory at the B3LYP/6-31G(d,p) level, and used to simulate coherent infrared vibrational spectra. 12 normal modes are included in the simulation, and the Arnoldi method is employed for the diagonalization of the Hamiltonian. The calculated three pulse infrared signals in the k1 + k2 - k3 direction show signatures of the intramolecular hydrogen bond couplings between the C=O stretch, H-O-C bend and O-H stretch vibrations.

• Journal of Nutrition and Health
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• 제23권6호
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• pp.434-442
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• 1990

과산화지질 반응 계에서 말론알데히드 정량을 위한 HPLC방법을 개발하였으며, 알파-,베타- 카로텐, 루테인 및 리코펜의 간 마이크로좀 시스템에서의 항산화작용을 새로 개발한 HPLC 방법과 TBA 값으로 비교하였다. 해동횟수에 따른 간 마이크로좀의 말론알데히드 형성능력을 비교하였다. 결과로서, 말론알데히드는 5$\cdot6분만에 확인되었으며, pack area와 농도는 직선 관계를 나타내었다. 해동횟수 증가에 따라 마이크로좀의 지질과 산화 능력은 감소되었으며, 이는 세포막의 파괴에 기인한것으로 사료된다. Fe$^{+3}$-ADP/NADPH와 paraquar/NADPH 반응계에서 리코펜, 루테인 및 알파-카로텐은 베타-카로텐이나 토코페롤보다 강력한 항산화효과를 나타내었다. Fe$^{+3}$-ADP/NADPH 반응 계에서 카로테노이드와 토코페롤의 과산화지질 형성 억제효과는 TBA 값과 HPLC에 의한 말론알데히드 정량에 의해 비교해볼때 유사한 효과를 나타내었다.

• 한국식품과학회지
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• 제1권1호
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• pp.45-50
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• 1969

자외부흡광 스펙트럼의 변화와 티 비 에이(TBA) 반응으로 살필 때, 수용액의 송에서의 말론알데하이드(malonaldehyde)는 과장 $260m/{\mu}$ 부근의 자외선조명에 의하여 광화학적 변화를 받는다. 이 용액에 아프라톡신(aflatoxin) 을 가하면 파장 $360m/{\mu}$ 부근의 광선에 의해서 이와 같은 변화가 재빨리 일어나며, 또 보통 형광등을 이용한 실험실의 조명에 의해서도 비록 그 변화는 느려지지만 마찬가지로 일어난다. 이과 같은 변화는 비가역적이며, 이 반응에서 아프라톡신은 다만 감광제로서의 역할을 한다. 또 이 반응의 기작은 말론알데하이드 분자의 평행적 이중결합체 형성에 의한 것 같다.

• 한국식품영양과학회지
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• 제26권5호
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• pp.822-826
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• 1997

This study attempted to establish the basic data for ensuring the safety of dried Alaska pollack. The changes of such compounds in the curse of drying as cholesterol, malonaldehyde, 7-ketocho-lesterol, 7$\alpha$-, 7$\beta$-hydroxycholesterol and 25-hydroxycholesterol were analyzed. The contensts of cholesterol decreased rapidly in the samples durint sun, hot-air and frozen drying and the decreased levels of cholesterol in dried products were about 134.0~191.3mg/100gas compared with 307.1mg/100g in raw samples. 4.8~6.1$\mu\textrm{g}$/100g in dried samples. In the raw samples, oxidized cholesterols were not detected hydroxycholesterol(34.1~41.7$\mu\textrm{g}$), 7$\beta$-hydroxycholesterol(26.8~40.2$\mu\textrm{g}$/g) and 25-hydroxycholesterol(0.3~1.3$\mu\textrm{g}$/g) were detected. Frozen drying was formed to be the most effective to minimize the formation of oxidized cholesterol in Alaska pollacks.

• Preventive Nutrition and Food Science
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• 제4권3호
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• pp.167-170
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• 1999

Various concentrations of malonaldehyde (MA) produced upon hydrolysis of 1, 1, 3,3-tetraethoxypropane (TEP) were reacted with 2-thiobarbituric acid (TBA)and th e contents of MA-TBA complex were measured both with spectrophotometer and high performance liquid chromatography (HPLC). As the concentrations of MA-TBA increased, their absorbances and the corresponding HPLC peak areas increased. The correlation coefficient between absorbances and HPLC peak areas of MA-TBA peaks from the other compounds and butanol extraction of the complex increased its recovery its recovery by 29.4% . Measurement of the content of MA-TBA complex for monitoring the development of lipid oxidation was proven to be successful with the use of high performance liquid chromatography.