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Glycogen Metabolism in Vibrio vulnificus Affected by malP and malQ

  • Han, Ah-Reum;Lee, Yeon-Ju;Wang, Tianshi;Kim, Jung-Wan
    • Microbiology and Biotechnology Letters
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    • v.46 no.1
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    • pp.29-39
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    • 2018
  • Vibrio vulnificus needs various responsive mechanisms to survive and transmit successfully in alternative niches of human and marine environments, and to ensure the acquisition of steady energy supply to facilitate such unique life style. The bacterium had genetic constitution very different from that of Escherichia coli regarding metabolism of glycogen, a major energy reserve. V. vulnificus accumulated more glycogen than other bacteria and at various levels according to culture medium and carbon source supplied in excess. Glycogen was accumulated to the highest level in Luria-Bertani (3.08 mg/mg protein) and heart infusion (4.30 mg/mg protein) complex media supplemented with 1% (w/v) maltodextrin at 3 h into the stationary phase. Regarding effect of carbon source, more glycogen was accumulated when maltodextrin (2.34 mg/mg protein) was added than when glucose or maltose (0.78.1-14 mg/mg protein) was added as an excessive carbon source to M9 minimal medium, suggesting that maltodextrin metabolism might affect glycogen metabolism very closely. These results were supported by the analysis using the malP (encoding a maltodextrin phosphorylase) and malQ (encoding a 4-${\alpha}$-glucanotransferase) mutants, which accumulated much less glycogen than wild type when either glucose or maltodextrin was supplied as an excessive carbon source, but at different levels (3.1-80.3% of wild type glycogen). Therefore, multiple pathways for glycogen metabolism were likely to function in V. vulnificus and that responding to maltodextrin might be more efficient in synthesizing glycogen. All of the glycogen samples from 3 V. vulnificus strains under various conditions showed a narrow side chain length distribution with short chains (G4-G6) as major ones. Not only the comparatively large accumulation volume but also the structure of glycogen in V. vulnificus, compared to other bacteria, may explain durability of the bacterium in external environment.

Geochemistry of the Daebo Granitic Batholith in the Central Ogcheon Belt, Korea : A Preliminary Report (중부 옥천대에 분포하는 대보 화강암질 저반의 화학조성 : 예비보고서)

  • Cheong, Chang-Sik;Chang, Ho-Wan
    • Economic and Environmental Geology
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    • v.29 no.4
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    • pp.483-493
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    • 1996
  • The tectonic environment and source characteristics of the Daebo granitic batholith in the central Ogcheon Belt were investigated based upon major and trace element geochemistry. The batholith is comprised of three granite types; a biotite granite (DBBG), K-feldspar megacryst-bearing biotite granite (DBKG), and a more mafic granodiorite (DBGD). The variations of Na and K in the granites can not be explained by simple fractional crystallization from the same primary magma. The irregular behavior of these alkali elements indicates a variety of source materials or incomplete mixing of different source materials. The large ion lithophile (LIL) element enrichment and low Ta/Hf ratios of the granites are typical characteristics of normal, calc-alkaline continental arc granitoids. Based upon REE patterns of the granites, it seems to be unreasonable to regard the felsic DBBG as a late stage differentiate formed by residual melts after the fractionation of major constituent minerals of the more mafic DBGD. Inconsistent variations in ${\varepsilon}_{Nd}(t)$ and LIL element concentrations of the granites preclude a mixing model between primitive melt and LIL element-enriched upper crustal materials. The irregular geochemical variation of the granites is taken to be largely inherited from an already heterogeneous source region.

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Freeze-Thaw Durability and Carbonation of Concrete Surface Protecting materials (콘크리트 표면보호재 종류에 따른 동결융해 및 중성화 내구특성)

  • Lee, Beung-Duk;Kim, Hyun-Joong;Kwon, Young-Rak;Kim, Sye-Jun
    • Proceedings of the Korea Concrete Institute Conference
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    • 2008.04a
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    • pp.593-596
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    • 2008
  • Domestic area of most be happened chloride deicer damage. Because daily mean temperature is below 0$^{\circ}C$ from the area of domestic most. Use of deicing chemicals has been and will continue to be a major part of concrete structure in the highway. Chloride-containing chemicals such as calcium chloride or rock salt are main deicers for the road. Extensive use of chloride deicers is, however, not only the source of substantial cost penalties due to their corrosive action and ability to deterioration roadway surface materials but also the source of environmental damages. Chloride-containing chemicals such as calcium chloride or rock salt are main deicers for the road. Extensive use of chloride deicers is, however, not only the source of substantial cost penalties due to their corrosive action and ability to deterioration roadway surface materials but also the source of environmental damages. In this study, Use of deicing chemicals has been and will continue to be a major part of highway freeze-thaw durability and carbonation of concrete surface protecting materials

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Implementations of Remote Sensing, GIS, and GPS for Water Resources and Water Quality Monitoring

  • Wu, Mu-Lin;Chen, Chiou-Hsiung;Liu, Shiu-Feng;Wey, Jiun-Sheng
    • Proceedings of the KSRS Conference
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    • 2003.11a
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    • pp.1191-1193
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    • 2003
  • Water quantity and quality monitoring at Taipei Watershed Management Bureau (WRATB) is not only a daily business but also a long term job. WRATB is responsible for providing high quality drinking water to about four millions population in Taipei. The quality of drinking water provided by WRATB is among one of the best in Taiwan. The total area is 717 square kilometers. The water resource pollution is usually divided into two categories, point source pollution and nonpoint source pollution. Garbage disposal is the most important component of the point source pollution, especially those by tourist during holidays and weekends. Pesticide pollution, fertilizer pollution, and natural pollution are the major contributions for nonpoint source pollution. The objective of this paper is to implement remote sensing, geographic information systems, and global positioning systems to monitor water quantity and water quality at WRATB. There are 12 water quality monitoring stations and four water gauge stations at WRATB. The coordinates of the 16 stations were determined by GPS devices and created into the base maps. MapObjects and visual BASIC were implemented to create application modules for water quality and quantity monitoring. Water quality of the two major watersheds at WRATB was put on Internet for public review monthly. The GIS software, ArcIMS, can put location maps and attributes of all 16 stations on Internet for general public review and technical implementations at WRATB. Inquiry and statistic charts automatic manipulations for the past 18 years are also available. Garbage disposal by community and tourist were also managed by GIS and GPS. The storage, collection, and transportation of garbage were reviewed by ArcMap file format. All garbage cart and garbage can at WRATB can be displayed on the base maps. Garbage disposal by tourist during holidays and weekends can be managed by a PDA with a GPS device and a digital camera. Man power allocation for tourist garbage disposal management can be done in an integration of GIS and GPS. Monitoring of water quality and quantity at WRATB can be done on Internet and by a PDA.

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Measurement of VOCs Concentrations at Jeonju Industrial Area and Emission Characteristics (전주공단지역의 주요VOCs 배출농도 측정 및 배출원별 특성 분석)

  • Kim, Deug-Soo;Yang, Go-Soo;Park, Bi-O
    • Journal of Environmental Science International
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    • v.16 no.3
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    • pp.299-310
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    • 2007
  • It will be necessary to make proper management plans to preserve the air quality in good level for the public. In order to make these plans, source information and detail emission inventories of the city and near industrial areas should be given. However, lack of the source measurements data makes us more difficult to complete the source inventory. VOC source Inventory could be utilized for the feasibility study to estimate the contribution of VOC sources presenting to the receptor such as residential area. It may give policy maker an idea how to control the air quality, and improve their social environment in the area. This study shows data that measured VOCs concentrations from the local industrial areas in Jeonju during from May 2005 to January 2006. The samples were collected from the near sources in 7 major factories in the industrial park as well as 5 general sources in near city Jeonju area to elucidate the abundances of speciated VOCs and their spacial and temporal distributions depending on source bases. Industrial sources are as follows; chemical, food, paper, wood, metal, non-metal (glass), and painting (coating) industries. The 5 general sources are sampled from tunnel, gasoline gas station, dry cleaning shop, printing (copy) shop, and road pavement working place in urban area. To understand the near source effect at receptor, samples from the 2 receptor sites (one is at center of the industrial complex and the other site is at distance residential area downwind from the center) were collected and analyzed for the comparison to source concentration. The mass contributions of the speciated VOC to total mass of VOCs measured from the different sources and ambient (2 receptors) were presented and discussed.

Effect of TiO Addition on Morphologies and Luminescence Properties of ZnO Crystals Fabricated by Vapor Transport Method (기상이동법에 의해 제조된 ZnO 결정의 형상 및 발광 특성에 미치는 TiO 첨가의 영향)

  • Lee, Geun-Hyoung
    • Korean Journal of Materials Research
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    • v.28 no.10
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    • pp.590-594
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    • 2018
  • ZnO micro/nanocrystals are formed by a vapor transport method. Mixtures of ZnO and TiO powders are used as the source materials. The TiO powder acts as a reducing agent to reduce the ZnO to Zn and plays an important role in the formation of ZnO micro/nanocrystals. The vapor transport process is carried out in air at atmospheric pressure. When the weight ratios of TiO to ZnO in the source material are lower than 1:2, no ZnO micro/nanocrystals are formed. However, when the ratios of TiO to ZnO in the source material are greater than 1:1, the ZnO crystals with one-dimensional wire morphology are formed. In the room temperature cathodoluminescence spectra of all the products, a strong ultraviolet emission centered at 380 nm is observed. As the ratio of TiO to ZnO in the source material increases from 1:2 to 1:1, the intensity ratio of ultraviolet to visible emission increases, suggesting that the crystallinity of the ZnO crystals is improved. Only the ultraviolet emission is observed for the ZnO crystals prepared using the source material with a TiO/ZnO ratio of 2:1.

Analysis and estimation of the BTEX using Canister­GC/MS in Seongseo Industrial Complex (Canister와 GC/MS를 이용한 대구성서산업단지의 BTEX의 분석 및 평가)

  • Kim Tae-Sub;Choi Sung-Woo
    • Journal of Environmental Science International
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    • v.14 no.3
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    • pp.351-358
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    • 2005
  • The research in this paper was carried out to examine the BTEX(Benzene, Toluene, Ethylbenzene, Xylene) concentrations in Seongseo Industrial Complex. These compounds are the major constituents, more than $60\%$ in composition of total VOCs, mainly charging in ambient air. BTEX samples were collected from the 38 sites, 10 for the source points and 18 for the boundary sites, and were analyzed by canister-GC/MS. The mean concentrations of BTEX were 33 ppbv for benzene, 214 ppbv for toluene, 89 ppbv for ethyl benzene, 77 ppbv for xylene. Among the BTEX, toluene had the highest concentration in the source points and boundary sites. In the source points, BTEX concentration of incineration facility for hazardous wastewater appeared highly in the range of 220­350 ppbv. BTEX concentrations in source boundary sites appeared in the order of toluene>ethylbenzene>xylene>benzene. As a result of the correlation analysis, the concentration of the source points was related to those of the boundary sites. Correlation of ethylbenzene and xylene was presented to 0.7991(P<0.0 1),\;0.6329(P<0.05) as the correlation coefficient, respectively.

VOCs Emission Characteristics and Mass Contribution Analysis at Wanju Industrial Area (완주지역의 VOCs 배출특성에 따른 공단지역과 일반지역의 기여도 분석)

  • Kim, Deug-Soo;Yang, Go-Soo;Park, Bi-O
    • Journal of Korean Society for Atmospheric Environment
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    • v.24 no.5
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    • pp.562-573
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    • 2008
  • Concentrations of volatile organic compounds (VOCs) measured from the local industrial sources in Wanju industrial complex during June $2007{\sim}January$ 2008. The samples were collected from the primary sources (6 emission points) in 4 major factories in Wanju industrial complex as well as two general sources in Wanju County to elucidate the abundances of speciated VOCs and their spacial and temporal distributions depending on source bases. Industrial sources are as follows; fabricated metal manufacture, motor vehicle manufacture, rubber and plastic manufacture, and chemical manufacture factories. Two general source samples were collected from gasoline gas station and dry cleaning shop in urban area. In order to understand the near source influence at receptor, samples from the two receptor sites (one is at center of the industrial complex and the other site is at distance residential area downwind from the center) were collected with sample canister, and analyzed by using GC/MSD. The concentrations from different sources were compared and discussed. The mass contributions of the speciated VOCs to total VOCs measured from industrial sources and ambient ai r at two receptors were presented and discussed.

Development of the vac Source Profile using Collinearity Test in the Yeosu Petrochemical Complex (여수석유화학산단의 공선성 시험을 이용한 VOC 오염원 분류표 개발)

  • Jeon Jun-Min;Hur Dang;Hwang In Jo;Kim Dong-Sul
    • Journal of Korean Society for Atmospheric Environment
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    • v.21 no.3
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    • pp.315-327
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    • 2005
  • The total of 35 target VOCs (volatile organic compounds), which were included in the TO-14, was selected to develop a VOCs' source profile matrix of the Yeosu Petrochemical Complex and to test its collinearity by singular value decomposition(SVD) technique. The VOCs collected in canisters were sampled from 12 different sources such as 8 direct emission sources (refinery, painting, wastewater treatment plant, incinerator, petrochemical processing, oil storage, fertilizer plant, and iron mill) and 4 general area sources (gasoline vapor emission, graphic art activity, vehicle emission, and asphalt paving activity) in this study area, and then those samples were analyzed by GC/MS. Initially the resulting raw data for each profile were scaled and normalized through several data treatment steps, and then specific VOCs showing major weight fractions were intensively reviewed and compared by introducing many other related studies. Next, all of the source profiles were tested in terms of degree of collinearity by SVD technique. The study finally could provide a proper VOCs' source profile in the study area, which can give opportunities to apply various receptor models properly including chemical mass balance (CMB).

An overview of Geothermal heat pumps as energy efficient and environmental friendly systems

  • Ahmad, Bilal;Kim, Dong-Hwan;Bahk, Sae-Mahn;Park, Myung-Kyun
    • Proceedings of the SAREK Conference
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    • 2008.06a
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    • pp.294-298
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    • 2008
  • The major threats that human being is facing nowadays are the Climate change, depletion of the fossil fuels at a rapid rate and energy costs. A significant portion of world energy consumption is consumed by domestic heating and cooling. And heat pumps, due to their higher utilization efficiencies as compared to conventional heating and cooling systems, offer an attractive solution to this problem. Among the types of heat pumps, the Geothermal heat pump or Ground-source heat pump is a highly efficient, renewable energy technology for space heating and cooling. The Ground-source heat pump uses the Earth as a heat sink in the summer and a heat source in the winter. And the Earth, having a relatively constant temperature, warmer than the air in winter and cooler than the air in summer, offers an excellent heat source in winter and heat sink in summer.. This paper will discuss an overview of the types of heat pumps, its operation, benefits of using geothermal heat pumps, soil characteristics, and overview of some experimental works. Finally it will briefly discuss the opportunity of using these energy efficient systems (EES) in the HVAC market of South Korea.

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