• Journal of Microbiology
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• v.42 no.2
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• pp.99-102
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• 2004

Bacteriophage PM2 has a closed circular form of double stranded DNA as a genome. This DNA from the phage is a useful source for nick-circle endonuclease assay in the fmol range. Due to difficulties in the maintenance of viral infectivity, storage conditions of the phage should be considered for the puri-fication of PM2 DNA. The proper condition for a short-term storage of less than 2 months is to keep the PM2 phage at 4$^{\circ}C$; whereas the proper condition for a long-term storage of the PM2 phage for over 2 months is to keep it under liquid nitrogen in 7.5 ％ glycerol. The optimal conditions for a high yield of phage progeny were also considered with the goal to achieve a successful PM2 DNA preparation. A MOI(Multiplicity Of Infection) of 0.03, in which the OD$\sub$600/ of the host bacteria was between 0.3 and 0.5, turned out to be optimal for the mass production of PM2 phage with a burst size of about 214. Considerations of PM2 genome size, and the concentrations and radiospecific activities of purified PM2 DNA, are required to measure the endonuclease activity in the fmol range. This study reports the proper quantitation of radioactivity and the yield of purified DNA based on these conditions.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.28 no.1A
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• pp.17-24
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• 2003

Access network is one of the most critical components of telecommunication networks. Essentially it provides the final connection through to the subscriber and at the same it is usually the most expensive component in terms of capital investment and ongoing cost of maintenance and repair In recent, various access technologies with broadband have been developed and also are emerging newly Comparing fixed access networks, the concrete concept as well as the scope of mobile access networks or parts has not been defined yet although it is caused by peculiarities of mobile network with mobility-guarantee technology, use of RF resource, fixed-exchange and transport networks This article examines the characteristics and detailed functions of BTS, BSC, MSC and other components in CDMA mobile network under the IS-95 A/B and cdma 2000-1x standards comparing the ITU's access network functional model From the systematic and functional perspectives, futhermore, an alternative definition for access parts of mobile network is proposed.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.41 no.4
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• pp.299-305
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• 2017

Re-engineering is gaining attention due to severe environmental pollution and economic crisis. Although re-engineering in shipbuilding has been carried out steadily, technological advancements, domestic ship repair and maintenance industries are not as prevalent as shipbuilding industries. In order to solve this problems, laser cladding can significantly aid with technical development. Laser cladding produces an outstanding clad layer with minimal dilution and little porosity. In this study, prior to applying laser cladding to an exhaust valve face, the effects of various parameters that affect the characteristics of the 1 pass clad layer were investigated. When laser power was increased, the clad layer width became broader and the height was decreased. In addition, it was identified that the hardness of the clad layer was inversely proportional to the power, and the entire clad layer was diluted through an EDS chemical composition analysis.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.40 no.12
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• pp.1040-1047
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• 2012

This paper presents a fault diagnosis algorithm of control surfaces of small fixed-wing aircraft to reduce maintenance cost or to improve repair efficiency by estimation of fault occurrence or part replacement periods. The proposed fault diagnosis algorithm consists of ANPSD (Averaged Normalized Power Spectral Density), PCA (Principle Component Analysis), and GC (Geometric Classifier). ANPSD is used for frequency-domain vibration testing. PCA has advantage to extract compressed information from ANPSD. GC has good properties to minimize errors of the fault detection and isolation. The algorithm was verified by the accelerometer measurements of the scaled normal and faulty ailerons and the test results show that the algorithm is suitable for the detection and isolation of the control surface faults. This paper also proposes solutions for some kind of implementation problems.

• The Journal of Korea Institute of Information, Electronics, and Communication Technology
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• v.11 no.5
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• pp.586-592
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• 2018

In this paper, HIC with various functions is proposed for the design 140W LED power control circuit. The proposed HIC integrates constant voltage/constant current circuit, short circuit protection circuit, internal constant voltage circuit, and dimmer circuit, thereby reducing the horizontal length of the PCB by 16% comparing with the conventional system. Through various experiments, we verified the performance of each block implemented inside of HIC with numerical results. (Constant voltage variation ratio: 2.9%, dimmer circuit duty variation within 5%, stable short protection at 720 mA) Since the PCB area can be significantly reduced by applying the proposed HIC. It is possible to reduce the PCB manufacturing time which takes up most of the manufacturing time, however, It is expected that the faulted power module can be replaced without replacing the whole PCB, so that maintenance / repair can be made easier.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.22 no.3
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• pp.411-417
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• 2018

This paper proposes and implements an on-line instruction manual system which can generate the instruction manual page for the target device at the smartphone on the spot. The instruction manual app. on smartphone scans a QR code or reads NFC tag attached in the instruction manual management module embedded in the target device, and receives instruction manual data from the instruction manual management module through the Bluetooth communications and finally shows the refined instruction manual pages on the smartphone display using a Web-based templates. For the evaluation we embedded the instruction manual management module for an industrial generator with its instruction manual data. Assuming a circumstance of blackout we show that the proposed system can reduce the repair work within two steps compared to three steps in the existing one without the proposed system.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.16 no.6
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• pp.4322-4327
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• 2015

Improper backfill materials and compaction controls under pipelines have become one of the major causes of failure in many sewage pipeline systems. A study on backfill materials and compaction controls has been considered for a long time. However, structural supporters under the pipe were recently concerned because of pipeline repair and maintenance. This paper presents a prefabricated plastic foundation for supporting a sewage pipe system and increasing the performance function of the pipes. Several analytical models for the plastic foundations were investigated using finite-element program, ABAQUS, for checking safety. Comparing with the results of analyses, some of economic design sections based on the sizes of pipe diameters, 600mm, 700 and 600mm, were evaluated. These results could be applied to a pipeline system with a prefabricated plastic foundation with respect to pipe diameters and buried depths.

• Journal of Fashion Business
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• v.12 no.3
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• pp.116-135
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• 2008

To investigate the actual distribution condition and clothing construction factors of the working clothes supplied to the Changwon national industrial complex, 5 major companies in machinery, automotive, industrial engineering, shipbuilding and rolling stock in the industrial complex located in Gyeongsangnam-Do were selected. The questionnaire designed for the research consisted of working clothes distribution policies in manufacturing industry and the actual conditions of the design facts, repair and maintenance of the working clothes, etc. The analysis of the clothing construction factors of the working clothes provided by 5 respondent companies were conducted in parallel. The results derived from the study were as follows: The basic types of working clothes were the blouson jacket and straight pants; safety equipments for manufacture were safety helmets, gloves, snickers, goggles, masks, ear caps, wristlets, leggings, apron, etc. The size-charts adopted by the participant companies were the small-medium-large and cm/inch measurement size systems. To solve wearer's dissatisfaction with the garment fit, certain clothing construction factors were used, e.g. strap bands and the elastic band on a waist band. The types of fabrics used for the working clothes were mainly polyester/cotton and polyester/rayon blended ones. Moreover, to provide with more air permeability to wearers, the warp knit material was used to construct the lining and the armpit or back bodice slits. Lock, two-thread chain, safety, overedge stitches were applied with flat, lap felled, French, superimposed, lapped, bound and edge finishing seams to construct the working clothes selected.

• Fire Science and Engineering
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• v.23 no.3
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• pp.110-120
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• 2009

In tunnel, though the frequency of fire occurrence is relatively lower than other structures, the characteristics of sealed space tends to cause the temperature to rapidly rise to more than $1000^{\circ}C$ within 5minutes after fire, which might eventually lead to a large fire that usually results in a loss of lives and the damage to the properties, not to mention a huge cost necessary for repair and maintenance after fire. We have developed various conditions of the heating furnace and the method to install a thermo couple within the furnace based on EFNARC and KS F 2257-1. Referring to tunnel fire scenarios, it clarified the heat transfer characteristics of concrete PC panel lining depending on fire intensity (ISO, $1^{\circ}C$/SEC, MHC, RWS), and to identify the range of thermal damage, the evaluation was carried out using ITA standard. As a result, 30mm under ISO fire condition, 20mm under $1^{\circ}C$/SEC, 100mm under MHC and 50mm under RWS were measured. And when it comes to spalling, 30mm was measured under RWS and MHC.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.12 no.11
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• pp.5123-5127
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• 2011

Research and development for the technology, that is about maintenance and accidents prevention of underground power distribution line, are demanding. The precise detection of leakage point of underground power line is very important, because it is difficult to detect the exact location of a fault in underground power line and to repair faults. When earth electric potential is measured to detect underground electric leakage point after transmitting AC electric pulse wave to underground power line, it must be measured in a specific half period of AC pulse wave because the distribution of the electric earth potential varies with the polarity of the transmitted wave. In this paper we proposed the measurement of half period modulated earth potential as a method to detect a underground leakage point. And We compared the proposed method with other methods. Through experiments we verified that the proposed method can be implemented and operated properly.