• Proceedings of the Korean Powder Metallurgy Institute Conference
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• 2006.09b
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• pp.1282-1283
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• 2006

• Proceedings of the Korean Magnetic Resonance Society Conference
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• 1999.02a
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• pp.22-22
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• 1999

• Proceedings of the Korean Magnetic Resonance Society Conference
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• 2002.08a
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• pp.87-87
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• 2002

• Bulletin of the Korean Chemical Society
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• v.35 no.9
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• pp.2753-2757
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• 2014

INtegrase Interactor 1 protein (INI1/hSNF5) or BRG1-associated factor 47 (BAF47) is a SWI/SNF-related matrix associated actin dependent regulator of chromatin subfamily B member. DNA binding domain of INI1/hSNF5 is cloned into E.coli expression vectors, pET32a and purified as a monomer using size exclusion chromatography. NMR data show that $INI1^{DBD}$ has folded state with high population of ${\alpha}$-helices. By fluorescence-quenching experiments, binding affinities between $INI1^{DBD}$ and two double stranded DNA fragments were determined as $29.9{\pm}2.6{\mu}M$ (GAL4_1) and $258.7{\pm}5.8$ (GAL4_2) ${\mu}M$, respectively. Our data revealed that DNA binding domain of INI1/hSNF5 binds to transcriptional DNA sequences, and it could play an important role as a transcriptional regulator.

• Journal of the Korean Magnetic Resonance Society
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• v.19 no.2
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• pp.95-98
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• 2015

Reverse gyrase is a hyperthermophile specific protein which introduces positive supercoils into DNA molecules. Reverse gyrase consists of an N-terminal helicase-like domain and a C-terminal topoisomerase domain. The helicase-like domain shares the three-dimensional structure with two tandem RecA-folds (H1 and H2), in which the subdomain H2 is interrupted by the latch domain (H3). To understand the physical property of the hyperthermophile-specific protein, two subdomains af_H1 and af_H23 have been cloned into E. coli expression vector, pET28a. The $^{15}N$-labeled af_H1 and af_H23 proteins were expressed and purified for heteronuclear NMR study. The af_H1 protein exhibits the well-dispersion of amide signals in its $^1H/^{15}N$-HSQC spectra and thus further NMR study continues to be progressed.

• Journal of the Korean Magnetic Resonance Society
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• v.11 no.1
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• pp.24-29
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• 2007

[ ${\beta}ig-h3$ ] is an extracellular matrix protein that mediates cell adhesion through interaction with integrins. The 18 residue YH motifs within each fas-1 domain are known to be responsible for the interaction with the ${\alpha}_v{\beta}_5$ integrin, and the synthetic YH motif peptides are known to inhibit endothelial tube formation and reduces the number of blood vessels, and so expected to be an effective inhibitor of angiogenesis. In this study, we solved the 3D structure of the 18 residue YH motif peptide (EALRDLLNNHILKSAMCA; D2 peptide) within the second fas-1 domain of ${\beta}ig-h3$ using NMR. The Peptide has ${\alpha}-helix$ structure at the C terminal region but the N terminal region is flexible. The present structural information may be helpful for developing more effective peptide drug candidate for the treatment of diseases dependent on angiogenesis.

• Journal of the Korean Magnetic Resonance Society
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• v.22 no.4
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• pp.119-124
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• 2018

Although the transcription factor CP2c has been recently validated as a promising target for development of novel anticancer therapy, its structure has not been solved yet. In the present study, the purified recombinant protein corresponding to the tetramerization domain of CP2c appeared to be well folded, whereas the Elf-1 domain showed a largely unfolded conformation. Particularly, the Elf-1 domain, which contains the putative DNA-binding region, showed a conformational equilibrium between relatively less-ordered and well-ordered conformers. Interestingly, addition of zinc shifted the equilibrium to the relatively more structured conformer, whereas zinc binding decreased the overall stability of the protein, leading to a promoted precipitation. Likewise, a dodecapeptide that has been suggested to bind to the Elf-1 domain also appeared to shift the conformational equilibrium and to destabilize the protein. These results constitute the first structural characterization of the CP2c domains and newly suggest that zinc ion might be involved in the conformational regulation of the protein.

• Journal of the Korean Magnetic Resonance Society
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• v.23 no.1
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• pp.20-25
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• 2019

The transcription factor CP2c has been recently validated as an oncogenic protein that can serve as a promising target for anticancer therapy. We have recently documented that a recombinant protein corresponding to the putative DNA-binding region (residues 63-244) of CP2c adopted two different conformers, one of which is dominated by zinc binding. However, in the present study, a longer construct encompassing residues 63-302 appeared to form a single structural domain. This domain could be considered to adopt a functionally relevant fold, as the known specific binding of a dodecapeptide to this protein was evident. Hence, the residues 63-302 region rather than 63-244 can be regarded as a natively folded structural domain of CP2c. In addition, it was confirmed that zinc ions can bind to this putative DNA-binding domain of CP2c, which resulted in reduced stability of the protein. In this context, it is suggested that the mode of action of CP2c would resemble that of tumor suppressor p53.

• Journal of Power Electronics
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• v.13 no.2
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• pp.275-286
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• 2013

An explicit frequency-domain circuit model for the conventional coupled magnetic resonance system (CMRS) is newly proposed in this paper. Detail circuit parameters such as the leakage inductances, magnetizing inductances, turn-ratios, internal coil resistances, and source/load resistances are explicitly included in the model. Accurate overall system efficiency, DC gain, and key design parameters are deduced from the model in closed form equations, which were not available in previous works. It has been found that the CMRS can be simply described by an equivalent voltage source, resistances, and ideal transformers when it is resonated to a specified frequency in the steady state. It has been identified that the voltage gain of the CMRS was saturated to a specific value although the source side or the load side coils were strongly coupled. The phase differences between adjacent coils were ${\pi}/2$, which should be considered for the EMF cancellations. The analysis results were verified by simulations and experiments. A detailed circuit-parameter-based model was verified by experiments for 500 kHz by using a new experimental kit with a class-E inverter. The experiments showed a transfer of 1.38 W and a 40 % coil to coil efficiency.

• The Bulletin of The Korean Astronomical Society
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• v.40 no.1
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• pp.67.1-67.1
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• 2015

In the past two decades, diverse methods and computer codes for reconstruction of coronal magnetic fields have been developed. Some of them can reproduce a known analytic solution quite well when the magnetic field vector is fully specified by the known solution at the domain boundaries. In practical problems, however, we do not know the boundary conditions in the computational domain except the photospheric boundary, where vector magnetogram data are provided. We have developed a new, simple variational method employing vector potentials. We have tested the computational code based on this method for problems with known solutions and those with actual photospheric data. When solutions are fully given at all boundaries, the accuracy of our method is almost comparable to best performing methods in the market. When magnetic field vectors are only given at the photospheric boundary, our method excels other methods in "figures of merit" devised by Schrijver et al. (2006). Our method is expected to contribute to the real time monitoring of the sun required for future space weather prediction.